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Kyuchang Wang - One of the best experts on this subject based on the ideXlab platform.

  • Neurosphere formation potential resides not in the Caudal Cell Mass, but in the secondary neural tube.
    The International Journal of Developmental Biology, 2017
    Co-Authors: Ji Yeoun Lee, Eun Sun Lee, Saet Pyoul Kim, Myung Sook Lee, Ji Hoon Phi, Seung-ki Kim, Young Il Hwang, Kyuchang Wang
    Abstract:

    The Caudal Cell Mass (CCM) is known as the main player in secondary neurulation, forming the secondary neural tube (2NT). This suggests that the CCM may have the character of neural progenitor Cells. The neural potential of the CCM and the 2NT (CCM + 2NT) was assessed by in vitro culture of neurospheres during Hamburger and Hamilton stages (HH) of secondary neurulation (HH16 to HH32). We also analyzed the neural potential of the developing central nervous system (CNS) by comparing the neurosphere culture from the brain, upper / lower spinal cord, and CCM + 2NT from various HH stages. The CCM + 2NT was capable of forming neurospheres. Of the various HH stages, the greatest number of neurospheres from CCM + 2NT were cultured at HH28. Because the 2NT is most prominent at HH28, we hypothesized that the 2NT, rather than the CCM, had the main potential to produce neurospheres. When the neurospheres were cultured separately from the CCM and the 2NT, 2NT made significantly more neurospheres. When comparing different parts of the CNS, at HH16, the greatest number of neurospheres was formed from the brain. At HH32, it was the CCM + 2NT. The region with the greatest number of neurospheres progressed in a cephalo-Caudal direction during development. This study showed that neurospheres can be cultured from CCM + 2NT, and the main player in neurosphere formation was the 2NT. The present study has also revealed cephalo-Caudal trend in the neural potential of developing CNS.

  • Secondary neurulation of human embryos: morphological changes and the expression of neuronal antigens
    Child's Nervous System, 2014
    Co-Authors: Hee Jin Yang, Kyuchang Wang
    Abstract:

    Purpose The morphological changes and expression patterns of neuronal antigens of human embryos, obtained from the therapeutic termination of pregnancy or from surgical procedures, were analyzed in order to characterize the secondary neurulation. Methods A total of 21 human embryos from Carnegie stages 12 to 23 and two fetuses in early stages were studied. The markers used for immunohistochemical study were neural Cell adhesion molecule (N-CAM), neuronal nuclear antigen (NeuN), neurofilament-associated protein (3A10), synaptophysin, and glial fibrillary acidic protein (GFAP). Results The formation of the Caudal neural tube to the tip of the Caudal portion of the embryo was finished at stage 17. The postcloacal gut had completely disappeared at stage 18, and multiple cavities of the Caudal neural tube were clearly visible. The Caudal portion of the neural tube showed findings suggestive of involution at stage 19. The expression patterns of neuronal antigens were as follows: N-CAM and NeuN showed immunoreactivity at the germinal layer of the spinal cord at stages 17 and 18. Neurofilament-associated protein (3A10) showed persistent immunoreactivity at the Caudal Cell Mass and notochord during the observation period, along with the spinal cord, and the positive reactions were mainly located at the dorsal white matter at stage 17. Synaptophysin showed a weak positive reaction at the Caudal Cell Mass and notochord at stages 13 and 14, evident by staining observed at the spinal cord at stages 15 and 16. There was no definite positive reaction for GFAP. Conclusions These characteristic patterns might be helpful for the understanding of human congenital anomalies involving secondary neurulation processes.

  • Spinal epidural teratoma: review of spinal teratoma with consideration on the pathogenesis: case report.
    Neurosurgery, 2010
    Co-Authors: Seong-cheol Park, Kyuchang Wang, Ki-jeong Kim, Gheeyoung Choe, Hyun-jib Kim
    Abstract:

    Of the 170 cases of teratomas in the spinal canal reported in the English literature, only 13 were spinal epidural teratomas (SETs). We present a case of SET, review the characteristics of SETs in comparison with spinal intradural teratomas (SITs), and investigate the pathogenesis of spinal teratoma. A 17-month-old boy visited our clinic with paraparesis. A multicystic Mass was noted in the left epidural space from T8 to T10 and the left paraspinal area. Complete resection of the tumor, including the paraspinal portion, was accomplished by laminoplastic laminotomy. The tumor was well encapsulated and filled with heterogeneous contents. The tumor was suspected to originate from the left T9 root. On histopathological examination, the tumor was found to be a mature teratoma. The clinical characteristics of SETs and SITs show similarities in age, male preponderance, location, associated anomalies, and pathology. SETs and SITs are probably 2 different types of a single disease entity. Pluripotent somatic Cell from the Hensen's node and Caudal Cell Mass is the probable origin of spinal teratoma. However, originating from misplaced primordial germ Cell could not be excluded.

  • Spinal epidural teratoma: review of spinal teratoma with consideration on the pathogenesis: case report.
    Neurosurgery, 2010
    Co-Authors: Seong-cheol Park, Kyuchang Wang, Ki-jeong Kim, Gheeyoung Choe, Hyun-jib Kim
    Abstract:

    Background and importance Of the 170 cases of teratomas in the spinal canal reported in the English literature, only 13 were spinal epidural teratomas (SETs). We present a case of SET, review the characteristics of SETs in comparison with spinal intradural teratomas (SITs), and investigate the pathogenesis of spinal teratoma. Clinical presentation A 17-month-old boy visited our clinic with paraparesis. A multicystic Mass was noted in the left epidural space from T8 to T10 and the left paraspinal area. Complete resection of the tumor, including the paraspinal portion, was accomplished by laminoplastic laminotomy. The tumor was well encapsulated and filled with heterogeneous contents. The tumor was suspected to originate from the left T9 root. Conclusion On histopathological examination, the tumor was found to be a mature teratoma. The clinical characteristics of SETs and SITs show similarities in age, male preponderance, location, associated anomalies, and pathology. SETs and SITs are probably 2 different types of a single disease entity. Pluripotent somatic Cell from the Hensen's node and Caudal Cell Mass is the probable origin of spinal teratoma. However, originating from misplaced primordial germ Cell could not be excluded.

  • Expression of neuronal markers in the secondary neurulation of chick embryos
    Child's Nervous System, 2008
    Co-Authors: Younam Chung, Hee Jin Yang, Myung Sook Lee, Seung-ki Kim, Do-hun Lee, Yun-jin Lee, Byung-kyu Cho, Dong-ho Kim, Kyuchang Wang
    Abstract:

    Objective The goal of our study was to evaluate the expression patterns of neuronal antigens during the process of secondary neurulation. Materials and methods Chick embryos of Hamburger and Hamilton stages 16, 18, 20, 22, 24, 26, 30, 35, 40, and 45 were harvested, and tail sections were processed for neuronal antigen studies. Results and conclusions The areas and periods showing positive reactions for each antigen are as follows: neuronal Cell adhesion molecule (N-CAM) : the secondary neural tube and notochord from stages 18 to 26 and the germinal and mantle layers from stages 30 to 45; synaptophysin : the Caudal Cell Mass, secondary neural tube, and notochord from stages 22 to 26, the germinal and mantle layers from stages 30 to 45, and the marginal layer at the later stages of development; neurofilament-associated protein (3A10) : the dorsal white matter, dorsal root ganglion, and scattered Cells around the germinal layer from stages 35 to 45; and neuronal nuclear-specific protein (NeuN) : the mantle layer at stage 35, which shows decreased reaction at stages 40 and 45; islet-1 : no remarkable staining on the Caudal Cell Mass or on the other neural structures at all stages. Our results indicate that neuronal markers of the secondary neurulation in chick embryos have their own chronological patterns of expression. At early stages of secondary neurulation, N-CAM and synaptophysin are thought to modulate the differentiation of structures derived from the Caudal Cell Mass. At later stages, N-CAM, synaptophysin, 3A10, and NeuN seem to be involved in the maturation of the Caudal spinal cord.

Hyun-jib Kim - One of the best experts on this subject based on the ideXlab platform.

  • Spinal epidural teratoma: review of spinal teratoma with consideration on the pathogenesis: case report.
    Neurosurgery, 2010
    Co-Authors: Seong-cheol Park, Kyuchang Wang, Ki-jeong Kim, Gheeyoung Choe, Hyun-jib Kim
    Abstract:

    Of the 170 cases of teratomas in the spinal canal reported in the English literature, only 13 were spinal epidural teratomas (SETs). We present a case of SET, review the characteristics of SETs in comparison with spinal intradural teratomas (SITs), and investigate the pathogenesis of spinal teratoma. A 17-month-old boy visited our clinic with paraparesis. A multicystic Mass was noted in the left epidural space from T8 to T10 and the left paraspinal area. Complete resection of the tumor, including the paraspinal portion, was accomplished by laminoplastic laminotomy. The tumor was well encapsulated and filled with heterogeneous contents. The tumor was suspected to originate from the left T9 root. On histopathological examination, the tumor was found to be a mature teratoma. The clinical characteristics of SETs and SITs show similarities in age, male preponderance, location, associated anomalies, and pathology. SETs and SITs are probably 2 different types of a single disease entity. Pluripotent somatic Cell from the Hensen's node and Caudal Cell Mass is the probable origin of spinal teratoma. However, originating from misplaced primordial germ Cell could not be excluded.

  • Spinal epidural teratoma: review of spinal teratoma with consideration on the pathogenesis: case report.
    Neurosurgery, 2010
    Co-Authors: Seong-cheol Park, Kyuchang Wang, Ki-jeong Kim, Gheeyoung Choe, Hyun-jib Kim
    Abstract:

    Background and importance Of the 170 cases of teratomas in the spinal canal reported in the English literature, only 13 were spinal epidural teratomas (SETs). We present a case of SET, review the characteristics of SETs in comparison with spinal intradural teratomas (SITs), and investigate the pathogenesis of spinal teratoma. Clinical presentation A 17-month-old boy visited our clinic with paraparesis. A multicystic Mass was noted in the left epidural space from T8 to T10 and the left paraspinal area. Complete resection of the tumor, including the paraspinal portion, was accomplished by laminoplastic laminotomy. The tumor was well encapsulated and filled with heterogeneous contents. The tumor was suspected to originate from the left T9 root. Conclusion On histopathological examination, the tumor was found to be a mature teratoma. The clinical characteristics of SETs and SITs show similarities in age, male preponderance, location, associated anomalies, and pathology. SETs and SITs are probably 2 different types of a single disease entity. Pluripotent somatic Cell from the Hensen's node and Caudal Cell Mass is the probable origin of spinal teratoma. However, originating from misplaced primordial germ Cell could not be excluded.

Seong-cheol Park - One of the best experts on this subject based on the ideXlab platform.

  • Spinal epidural teratoma: review of spinal teratoma with consideration on the pathogenesis: case report.
    Neurosurgery, 2010
    Co-Authors: Seong-cheol Park, Kyuchang Wang, Ki-jeong Kim, Gheeyoung Choe, Hyun-jib Kim
    Abstract:

    Of the 170 cases of teratomas in the spinal canal reported in the English literature, only 13 were spinal epidural teratomas (SETs). We present a case of SET, review the characteristics of SETs in comparison with spinal intradural teratomas (SITs), and investigate the pathogenesis of spinal teratoma. A 17-month-old boy visited our clinic with paraparesis. A multicystic Mass was noted in the left epidural space from T8 to T10 and the left paraspinal area. Complete resection of the tumor, including the paraspinal portion, was accomplished by laminoplastic laminotomy. The tumor was well encapsulated and filled with heterogeneous contents. The tumor was suspected to originate from the left T9 root. On histopathological examination, the tumor was found to be a mature teratoma. The clinical characteristics of SETs and SITs show similarities in age, male preponderance, location, associated anomalies, and pathology. SETs and SITs are probably 2 different types of a single disease entity. Pluripotent somatic Cell from the Hensen's node and Caudal Cell Mass is the probable origin of spinal teratoma. However, originating from misplaced primordial germ Cell could not be excluded.

  • Spinal epidural teratoma: review of spinal teratoma with consideration on the pathogenesis: case report.
    Neurosurgery, 2010
    Co-Authors: Seong-cheol Park, Kyuchang Wang, Ki-jeong Kim, Gheeyoung Choe, Hyun-jib Kim
    Abstract:

    Background and importance Of the 170 cases of teratomas in the spinal canal reported in the English literature, only 13 were spinal epidural teratomas (SETs). We present a case of SET, review the characteristics of SETs in comparison with spinal intradural teratomas (SITs), and investigate the pathogenesis of spinal teratoma. Clinical presentation A 17-month-old boy visited our clinic with paraparesis. A multicystic Mass was noted in the left epidural space from T8 to T10 and the left paraspinal area. Complete resection of the tumor, including the paraspinal portion, was accomplished by laminoplastic laminotomy. The tumor was well encapsulated and filled with heterogeneous contents. The tumor was suspected to originate from the left T9 root. Conclusion On histopathological examination, the tumor was found to be a mature teratoma. The clinical characteristics of SETs and SITs show similarities in age, male preponderance, location, associated anomalies, and pathology. SETs and SITs are probably 2 different types of a single disease entity. Pluripotent somatic Cell from the Hensen's node and Caudal Cell Mass is the probable origin of spinal teratoma. However, originating from misplaced primordial germ Cell could not be excluded.

Gheeyoung Choe - One of the best experts on this subject based on the ideXlab platform.

  • Spinal epidural teratoma: review of spinal teratoma with consideration on the pathogenesis: case report.
    Neurosurgery, 2010
    Co-Authors: Seong-cheol Park, Kyuchang Wang, Ki-jeong Kim, Gheeyoung Choe, Hyun-jib Kim
    Abstract:

    Of the 170 cases of teratomas in the spinal canal reported in the English literature, only 13 were spinal epidural teratomas (SETs). We present a case of SET, review the characteristics of SETs in comparison with spinal intradural teratomas (SITs), and investigate the pathogenesis of spinal teratoma. A 17-month-old boy visited our clinic with paraparesis. A multicystic Mass was noted in the left epidural space from T8 to T10 and the left paraspinal area. Complete resection of the tumor, including the paraspinal portion, was accomplished by laminoplastic laminotomy. The tumor was well encapsulated and filled with heterogeneous contents. The tumor was suspected to originate from the left T9 root. On histopathological examination, the tumor was found to be a mature teratoma. The clinical characteristics of SETs and SITs show similarities in age, male preponderance, location, associated anomalies, and pathology. SETs and SITs are probably 2 different types of a single disease entity. Pluripotent somatic Cell from the Hensen's node and Caudal Cell Mass is the probable origin of spinal teratoma. However, originating from misplaced primordial germ Cell could not be excluded.

  • Spinal epidural teratoma: review of spinal teratoma with consideration on the pathogenesis: case report.
    Neurosurgery, 2010
    Co-Authors: Seong-cheol Park, Kyuchang Wang, Ki-jeong Kim, Gheeyoung Choe, Hyun-jib Kim
    Abstract:

    Background and importance Of the 170 cases of teratomas in the spinal canal reported in the English literature, only 13 were spinal epidural teratomas (SETs). We present a case of SET, review the characteristics of SETs in comparison with spinal intradural teratomas (SITs), and investigate the pathogenesis of spinal teratoma. Clinical presentation A 17-month-old boy visited our clinic with paraparesis. A multicystic Mass was noted in the left epidural space from T8 to T10 and the left paraspinal area. Complete resection of the tumor, including the paraspinal portion, was accomplished by laminoplastic laminotomy. The tumor was well encapsulated and filled with heterogeneous contents. The tumor was suspected to originate from the left T9 root. Conclusion On histopathological examination, the tumor was found to be a mature teratoma. The clinical characteristics of SETs and SITs show similarities in age, male preponderance, location, associated anomalies, and pathology. SETs and SITs are probably 2 different types of a single disease entity. Pluripotent somatic Cell from the Hensen's node and Caudal Cell Mass is the probable origin of spinal teratoma. However, originating from misplaced primordial germ Cell could not be excluded.

Ki-jeong Kim - One of the best experts on this subject based on the ideXlab platform.

  • Spinal epidural teratoma: review of spinal teratoma with consideration on the pathogenesis: case report.
    Neurosurgery, 2010
    Co-Authors: Seong-cheol Park, Kyuchang Wang, Ki-jeong Kim, Gheeyoung Choe, Hyun-jib Kim
    Abstract:

    Of the 170 cases of teratomas in the spinal canal reported in the English literature, only 13 were spinal epidural teratomas (SETs). We present a case of SET, review the characteristics of SETs in comparison with spinal intradural teratomas (SITs), and investigate the pathogenesis of spinal teratoma. A 17-month-old boy visited our clinic with paraparesis. A multicystic Mass was noted in the left epidural space from T8 to T10 and the left paraspinal area. Complete resection of the tumor, including the paraspinal portion, was accomplished by laminoplastic laminotomy. The tumor was well encapsulated and filled with heterogeneous contents. The tumor was suspected to originate from the left T9 root. On histopathological examination, the tumor was found to be a mature teratoma. The clinical characteristics of SETs and SITs show similarities in age, male preponderance, location, associated anomalies, and pathology. SETs and SITs are probably 2 different types of a single disease entity. Pluripotent somatic Cell from the Hensen's node and Caudal Cell Mass is the probable origin of spinal teratoma. However, originating from misplaced primordial germ Cell could not be excluded.

  • Spinal epidural teratoma: review of spinal teratoma with consideration on the pathogenesis: case report.
    Neurosurgery, 2010
    Co-Authors: Seong-cheol Park, Kyuchang Wang, Ki-jeong Kim, Gheeyoung Choe, Hyun-jib Kim
    Abstract:

    Background and importance Of the 170 cases of teratomas in the spinal canal reported in the English literature, only 13 were spinal epidural teratomas (SETs). We present a case of SET, review the characteristics of SETs in comparison with spinal intradural teratomas (SITs), and investigate the pathogenesis of spinal teratoma. Clinical presentation A 17-month-old boy visited our clinic with paraparesis. A multicystic Mass was noted in the left epidural space from T8 to T10 and the left paraspinal area. Complete resection of the tumor, including the paraspinal portion, was accomplished by laminoplastic laminotomy. The tumor was well encapsulated and filled with heterogeneous contents. The tumor was suspected to originate from the left T9 root. Conclusion On histopathological examination, the tumor was found to be a mature teratoma. The clinical characteristics of SETs and SITs show similarities in age, male preponderance, location, associated anomalies, and pathology. SETs and SITs are probably 2 different types of a single disease entity. Pluripotent somatic Cell from the Hensen's node and Caudal Cell Mass is the probable origin of spinal teratoma. However, originating from misplaced primordial germ Cell could not be excluded.