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Franca Ronchese - One of the best experts on this subject based on the ideXlab platform.

  • long term deposition of inhaled antigen in lung resident cd11b CD11c cells
    American Journal of Respiratory Cell and Molecular Biology, 2007
    Co-Authors: Kate E Matthews, Adela Karabeg, Joanna M Roberts, Sem Saeland, Gerhard Dekan, Michelle M Epstein, Franca Ronchese
    Abstract:

    In this study we report the characterization of a population of lung resident CD11b−CD11c+ cells that are able to take up inhaled antigen and retain it for extended periods of time. Ovalbumin conjugated to fluorescein-isothiocyanate (FITC-OVA) administered intranasally to mice was taken up by two main populations of cells in the lung, a migratory CD11c+CD11b+ population consisting of dendritic cells (DC), which rapidly transported antigen to the draining lymph node (LN), and a resident CD11b−CD11c+ population that retained engulfed antigen without apparently degrading it for up to 8 wk after administration. The FITC+CD11b−CD11c+ cells did not migrate to draining LN at a detectable rate, and did not up-regulate expression of costimulatory molecules in response to LPS treatment. FITC+CD11b−CD11c+ cells were found in the lung and bronchoalveolar lavage fluid, and their distribution was compatible with macrophages. Although FITC+CD11b−CD11c+ cells expressed the DC marker DEC205 and other molecules associated ...

  • Long-Term Deposition of Inhaled Antigen in Lung Resident CD11b−CD11c+ Cells
    American journal of respiratory cell and molecular biology, 2006
    Co-Authors: Kate E Matthews, Adela Karabeg, Joanna M Roberts, Sem Saeland, Gerhard Dekan, Michelle M Epstein, Franca Ronchese
    Abstract:

    In this study we report the characterization of a population of lung resident CD11b−CD11c+ cells that are able to take up inhaled antigen and retain it for extended periods of time. Ovalbumin conjugated to fluorescein-isothiocyanate (FITC-OVA) administered intranasally to mice was taken up by two main populations of cells in the lung, a migratory CD11c+CD11b+ population consisting of dendritic cells (DC), which rapidly transported antigen to the draining lymph node (LN), and a resident CD11b−CD11c+ population that retained engulfed antigen without apparently degrading it for up to 8 wk after administration. The FITC+CD11b−CD11c+ cells did not migrate to draining LN at a detectable rate, and did not up-regulate expression of costimulatory molecules in response to LPS treatment. FITC+CD11b−CD11c+ cells were found in the lung and bronchoalveolar lavage fluid, and their distribution was compatible with macrophages. Although FITC+CD11b−CD11c+ cells expressed the DC marker DEC205 and other molecules associated ...

Kate E Matthews - One of the best experts on this subject based on the ideXlab platform.

  • long term deposition of inhaled antigen in lung resident cd11b CD11c cells
    American Journal of Respiratory Cell and Molecular Biology, 2007
    Co-Authors: Kate E Matthews, Adela Karabeg, Joanna M Roberts, Sem Saeland, Gerhard Dekan, Michelle M Epstein, Franca Ronchese
    Abstract:

    In this study we report the characterization of a population of lung resident CD11b−CD11c+ cells that are able to take up inhaled antigen and retain it for extended periods of time. Ovalbumin conjugated to fluorescein-isothiocyanate (FITC-OVA) administered intranasally to mice was taken up by two main populations of cells in the lung, a migratory CD11c+CD11b+ population consisting of dendritic cells (DC), which rapidly transported antigen to the draining lymph node (LN), and a resident CD11b−CD11c+ population that retained engulfed antigen without apparently degrading it for up to 8 wk after administration. The FITC+CD11b−CD11c+ cells did not migrate to draining LN at a detectable rate, and did not up-regulate expression of costimulatory molecules in response to LPS treatment. FITC+CD11b−CD11c+ cells were found in the lung and bronchoalveolar lavage fluid, and their distribution was compatible with macrophages. Although FITC+CD11b−CD11c+ cells expressed the DC marker DEC205 and other molecules associated ...

  • Long-Term Deposition of Inhaled Antigen in Lung Resident CD11b−CD11c+ Cells
    American journal of respiratory cell and molecular biology, 2006
    Co-Authors: Kate E Matthews, Adela Karabeg, Joanna M Roberts, Sem Saeland, Gerhard Dekan, Michelle M Epstein, Franca Ronchese
    Abstract:

    In this study we report the characterization of a population of lung resident CD11b−CD11c+ cells that are able to take up inhaled antigen and retain it for extended periods of time. Ovalbumin conjugated to fluorescein-isothiocyanate (FITC-OVA) administered intranasally to mice was taken up by two main populations of cells in the lung, a migratory CD11c+CD11b+ population consisting of dendritic cells (DC), which rapidly transported antigen to the draining lymph node (LN), and a resident CD11b−CD11c+ population that retained engulfed antigen without apparently degrading it for up to 8 wk after administration. The FITC+CD11b−CD11c+ cells did not migrate to draining LN at a detectable rate, and did not up-regulate expression of costimulatory molecules in response to LPS treatment. FITC+CD11b−CD11c+ cells were found in the lung and bronchoalveolar lavage fluid, and their distribution was compatible with macrophages. Although FITC+CD11b−CD11c+ cells expressed the DC marker DEC205 and other molecules associated ...

Michelle M Epstein - One of the best experts on this subject based on the ideXlab platform.

  • long term deposition of inhaled antigen in lung resident cd11b CD11c cells
    American Journal of Respiratory Cell and Molecular Biology, 2007
    Co-Authors: Kate E Matthews, Adela Karabeg, Joanna M Roberts, Sem Saeland, Gerhard Dekan, Michelle M Epstein, Franca Ronchese
    Abstract:

    In this study we report the characterization of a population of lung resident CD11b−CD11c+ cells that are able to take up inhaled antigen and retain it for extended periods of time. Ovalbumin conjugated to fluorescein-isothiocyanate (FITC-OVA) administered intranasally to mice was taken up by two main populations of cells in the lung, a migratory CD11c+CD11b+ population consisting of dendritic cells (DC), which rapidly transported antigen to the draining lymph node (LN), and a resident CD11b−CD11c+ population that retained engulfed antigen without apparently degrading it for up to 8 wk after administration. The FITC+CD11b−CD11c+ cells did not migrate to draining LN at a detectable rate, and did not up-regulate expression of costimulatory molecules in response to LPS treatment. FITC+CD11b−CD11c+ cells were found in the lung and bronchoalveolar lavage fluid, and their distribution was compatible with macrophages. Although FITC+CD11b−CD11c+ cells expressed the DC marker DEC205 and other molecules associated ...

  • Long-Term Deposition of Inhaled Antigen in Lung Resident CD11b−CD11c+ Cells
    American journal of respiratory cell and molecular biology, 2006
    Co-Authors: Kate E Matthews, Adela Karabeg, Joanna M Roberts, Sem Saeland, Gerhard Dekan, Michelle M Epstein, Franca Ronchese
    Abstract:

    In this study we report the characterization of a population of lung resident CD11b−CD11c+ cells that are able to take up inhaled antigen and retain it for extended periods of time. Ovalbumin conjugated to fluorescein-isothiocyanate (FITC-OVA) administered intranasally to mice was taken up by two main populations of cells in the lung, a migratory CD11c+CD11b+ population consisting of dendritic cells (DC), which rapidly transported antigen to the draining lymph node (LN), and a resident CD11b−CD11c+ population that retained engulfed antigen without apparently degrading it for up to 8 wk after administration. The FITC+CD11b−CD11c+ cells did not migrate to draining LN at a detectable rate, and did not up-regulate expression of costimulatory molecules in response to LPS treatment. FITC+CD11b−CD11c+ cells were found in the lung and bronchoalveolar lavage fluid, and their distribution was compatible with macrophages. Although FITC+CD11b−CD11c+ cells expressed the DC marker DEC205 and other molecules associated ...

Gerhard Dekan - One of the best experts on this subject based on the ideXlab platform.

  • long term deposition of inhaled antigen in lung resident cd11b CD11c cells
    American Journal of Respiratory Cell and Molecular Biology, 2007
    Co-Authors: Kate E Matthews, Adela Karabeg, Joanna M Roberts, Sem Saeland, Gerhard Dekan, Michelle M Epstein, Franca Ronchese
    Abstract:

    In this study we report the characterization of a population of lung resident CD11b−CD11c+ cells that are able to take up inhaled antigen and retain it for extended periods of time. Ovalbumin conjugated to fluorescein-isothiocyanate (FITC-OVA) administered intranasally to mice was taken up by two main populations of cells in the lung, a migratory CD11c+CD11b+ population consisting of dendritic cells (DC), which rapidly transported antigen to the draining lymph node (LN), and a resident CD11b−CD11c+ population that retained engulfed antigen without apparently degrading it for up to 8 wk after administration. The FITC+CD11b−CD11c+ cells did not migrate to draining LN at a detectable rate, and did not up-regulate expression of costimulatory molecules in response to LPS treatment. FITC+CD11b−CD11c+ cells were found in the lung and bronchoalveolar lavage fluid, and their distribution was compatible with macrophages. Although FITC+CD11b−CD11c+ cells expressed the DC marker DEC205 and other molecules associated ...

  • Long-Term Deposition of Inhaled Antigen in Lung Resident CD11b−CD11c+ Cells
    American journal of respiratory cell and molecular biology, 2006
    Co-Authors: Kate E Matthews, Adela Karabeg, Joanna M Roberts, Sem Saeland, Gerhard Dekan, Michelle M Epstein, Franca Ronchese
    Abstract:

    In this study we report the characterization of a population of lung resident CD11b−CD11c+ cells that are able to take up inhaled antigen and retain it for extended periods of time. Ovalbumin conjugated to fluorescein-isothiocyanate (FITC-OVA) administered intranasally to mice was taken up by two main populations of cells in the lung, a migratory CD11c+CD11b+ population consisting of dendritic cells (DC), which rapidly transported antigen to the draining lymph node (LN), and a resident CD11b−CD11c+ population that retained engulfed antigen without apparently degrading it for up to 8 wk after administration. The FITC+CD11b−CD11c+ cells did not migrate to draining LN at a detectable rate, and did not up-regulate expression of costimulatory molecules in response to LPS treatment. FITC+CD11b−CD11c+ cells were found in the lung and bronchoalveolar lavage fluid, and their distribution was compatible with macrophages. Although FITC+CD11b−CD11c+ cells expressed the DC marker DEC205 and other molecules associated ...

Sem Saeland - One of the best experts on this subject based on the ideXlab platform.

  • long term deposition of inhaled antigen in lung resident cd11b CD11c cells
    American Journal of Respiratory Cell and Molecular Biology, 2007
    Co-Authors: Kate E Matthews, Adela Karabeg, Joanna M Roberts, Sem Saeland, Gerhard Dekan, Michelle M Epstein, Franca Ronchese
    Abstract:

    In this study we report the characterization of a population of lung resident CD11b−CD11c+ cells that are able to take up inhaled antigen and retain it for extended periods of time. Ovalbumin conjugated to fluorescein-isothiocyanate (FITC-OVA) administered intranasally to mice was taken up by two main populations of cells in the lung, a migratory CD11c+CD11b+ population consisting of dendritic cells (DC), which rapidly transported antigen to the draining lymph node (LN), and a resident CD11b−CD11c+ population that retained engulfed antigen without apparently degrading it for up to 8 wk after administration. The FITC+CD11b−CD11c+ cells did not migrate to draining LN at a detectable rate, and did not up-regulate expression of costimulatory molecules in response to LPS treatment. FITC+CD11b−CD11c+ cells were found in the lung and bronchoalveolar lavage fluid, and their distribution was compatible with macrophages. Although FITC+CD11b−CD11c+ cells expressed the DC marker DEC205 and other molecules associated ...

  • Long-Term Deposition of Inhaled Antigen in Lung Resident CD11b−CD11c+ Cells
    American journal of respiratory cell and molecular biology, 2006
    Co-Authors: Kate E Matthews, Adela Karabeg, Joanna M Roberts, Sem Saeland, Gerhard Dekan, Michelle M Epstein, Franca Ronchese
    Abstract:

    In this study we report the characterization of a population of lung resident CD11b−CD11c+ cells that are able to take up inhaled antigen and retain it for extended periods of time. Ovalbumin conjugated to fluorescein-isothiocyanate (FITC-OVA) administered intranasally to mice was taken up by two main populations of cells in the lung, a migratory CD11c+CD11b+ population consisting of dendritic cells (DC), which rapidly transported antigen to the draining lymph node (LN), and a resident CD11b−CD11c+ population that retained engulfed antigen without apparently degrading it for up to 8 wk after administration. The FITC+CD11b−CD11c+ cells did not migrate to draining LN at a detectable rate, and did not up-regulate expression of costimulatory molecules in response to LPS treatment. FITC+CD11b−CD11c+ cells were found in the lung and bronchoalveolar lavage fluid, and their distribution was compatible with macrophages. Although FITC+CD11b−CD11c+ cells expressed the DC marker DEC205 and other molecules associated ...