The Experts below are selected from a list of 94644 Experts worldwide ranked by ideXlab platform
Peter J Polverini - One of the best experts on this subject based on the ideXlab platform.
-
angiogenesis mediated by soluble forms of e selectin and vascular Cell Adhesion Molecule 1
Nature, 1995Co-Authors: Alisa E Koch, Catherine J Haskell, Manisha R Shah, Margaret M Halloran, Peter J PolveriniAbstract:ENDOTHELIAL Adhesion Molecules facilitate the entry of leukocytes into inflamed tissues. This in turn promotes neovascularization, a process central to the progression of rheumatoid arthritis, tumour growth and wound repair1. Here we test the hypothesis that soluble endothelial Adhesion Molecules promote angiogenesis2a¤-4. Human recombinant soluble E-selectin and soluble vascular Cell Adhesion Molecule-1 induced chemotaxis of human endothelial Cells in vitro and were angiogenic in rat cornea. Soluble E-selectin acted on endothelial Cells in part through a sialyl Lewis-X-dependent mechanism, while soluble vascular Cell Adhesion Molecule-1 acted on endothelial Cells in part through a very late antigen (VLA)-4 dependent mechanism. The chemotactic activity of rheumatoid synovial fluid for endothelial Cells, and also its angiogenic activity, were blocked by antibodies to either soluble E-selectin or soluble vascular Cell Adhesion Molecule-1. These results suggest a novel function for soluble endothelial Adhesion Molecules as mediators of angiogenesis.
Alisa E Koch - One of the best experts on this subject based on the ideXlab platform.
-
angiogenesis mediated by soluble forms of e selectin and vascular Cell Adhesion Molecule 1
Nature, 1995Co-Authors: Alisa E Koch, Catherine J Haskell, Manisha R Shah, Margaret M Halloran, Peter J PolveriniAbstract:ENDOTHELIAL Adhesion Molecules facilitate the entry of leukocytes into inflamed tissues. This in turn promotes neovascularization, a process central to the progression of rheumatoid arthritis, tumour growth and wound repair1. Here we test the hypothesis that soluble endothelial Adhesion Molecules promote angiogenesis2a¤-4. Human recombinant soluble E-selectin and soluble vascular Cell Adhesion Molecule-1 induced chemotaxis of human endothelial Cells in vitro and were angiogenic in rat cornea. Soluble E-selectin acted on endothelial Cells in part through a sialyl Lewis-X-dependent mechanism, while soluble vascular Cell Adhesion Molecule-1 acted on endothelial Cells in part through a very late antigen (VLA)-4 dependent mechanism. The chemotactic activity of rheumatoid synovial fluid for endothelial Cells, and also its angiogenic activity, were blocked by antibodies to either soluble E-selectin or soluble vascular Cell Adhesion Molecule-1. These results suggest a novel function for soluble endothelial Adhesion Molecules as mediators of angiogenesis.
Vincenzo Lo Cascio - One of the best experts on this subject based on the ideXlab platform.
-
antioxidants inhibit the expression of interCellular Cell Adhesion Molecule 1 and vascular Cell Adhesion Molecule 1 induced by oxidized ldl on human umbilical vein endothelial Cells
Free Radical Biology and Medicine, 1997Co-Authors: Luciano Cominacini, Ulisse Garbin, Anna Fratta Pasini, A Davoli, M Campagnola, Giovanni B Contessi, A M Pastorino, Vincenzo Lo CascioAbstract:Abstract The oxidative modification of low density lipoprotein (LDL) and the endothelial expression of Adhesion Molecules are key events in the pathogenesis of atherosclerosis. In this study we evaluated the effect of oxidized LDL on the expression of interCellular Cell Adhesion Molecule-1 (ICAM-1), vascular Cell Adhesion Molecule-1 (VCAM-1), and E-selectin on human umbilical vein endothelial Cells (HUVECs). The hypothesis that oxidized LDL functions as a prooxidant signal was also evaluated, by studying the effect of different radical-scavenging antioxidants on expression of Adhesion Molecules. LDL was oxidized by using Cu2+, HUVECs or phospholipase A2 (PLA2)/soybean lipoxygenase (SLO), the degree of oxidation being measured as thiobarbituric acid-reactive substances (TBARS) and conjugated dienes (CD). Exposure of 200 μg/ml of native LDL to 1 μm Cu2+, HUVECs and to PLA2/SLO resulted in four- to fivefold higher levels of TBARS and CD than in native LDL. Cu2+- (1 μM), HUVEC-, and PLA2/SLO-oxidized LDL caused a dose-dependent, significant increase of ICAM-1 and VCAM-1 (p
S J Mentzer - One of the best experts on this subject based on the ideXlab platform.
-
Cell Adhesion Molecule expression in the sheep thymus.
Developmental and comparative immunology, 2020Co-Authors: T Zhao, C He, M Su, C A West, S J Swanson, A J Young, S J MentzerAbstract:Cell Adhesion Molecules are potential regulating factors in both prethymic and intrathymic T Cell development. An experimental challenge has been the development of a large animal model that facilitates in vivo studies of both intrathymic development and lymphocyte migration. To extend earlier studies of thymic development, we have developed a panel of monoclonal antibodies (mAb) to a variety of sheep Cell Adhesion Molecules. Immunohistochemistry was used to define mAb reactivity and flow cytometry was used to quantify expression of Cell Adhesion Molecules within the thymus. To facilitate flow cytometry definition of cortical thymocytes, mAbs were developed to the sheep CD1 antigen. Dual parameter flow cytometry provided a phenotypic characterization of Cell Adhesion Molecule expression on both CD1(+) and CD1(-) sheep thymocyte populations. These studies demonstrated significantly enhanced cortical thymocyte expression of three Cell Adhesion Molecules: beta1 integrin (CD29), ICAM-2 and LFA-3. The beta1 integrin Cell Adhesion Molecule was also expressed at higher levels on CD1(+) thymocytes in post-natal lambs as compared to adult sheep. These studies of thymocyte membrane Molecule expression should facilitate future investigations of sheep intrathymic development and T lymphocyte immigration.
-
Cell Adhesion Molecule expression in the sheep thymus.
Developmental and Comparative Immunology, 2001Co-Authors: T Zhao, C He, M Su, C A West, S J Swanson, A J Young, S J MentzerAbstract:Abstract Cell Adhesion Molecules are potential regulating factors in both prethymic and intrathymic T Cell development. An experimental challenge has been the development of a large animal model that facilitates in vivo studies of both intrathymic development and lymphocyte migration. To extend earlier studies of thymic development, we have developed a panel of monoclonal antibodies (mAb) to a variety of sheep Cell Adhesion Molecules. Immunohistochemistry was used to define mAb reactivity and flow cytometry was used to quantify expression of Cell Adhesion Molecules within the thymus. To facilitate flow cytometry definition of cortical thymocytes, mAbs were developed to the sheep CD1 antigen. Dual parameter flow cytometry provided a phenotypic characterization of Cell Adhesion Molecule expression on both CD1 + and CD1 − sheep thymocyte populations. These studies demonstrated significantly enhanced cortical thymocyte expression of three Cell Adhesion Molecules: β1 integrin (CD29), ICAM-2 and LFA-3. The β1 integrin Cell Adhesion Molecule was also expressed at higher levels on CD1 + thymocytes in post-natal lambs as compared to adult sheep. These studies of thymocyte membrane Molecule expression should facilitate future investigations of sheep intrathymic development and T lymphocyte immigration.
Margaret M Halloran - One of the best experts on this subject based on the ideXlab platform.
-
angiogenesis mediated by soluble forms of e selectin and vascular Cell Adhesion Molecule 1
Nature, 1995Co-Authors: Alisa E Koch, Catherine J Haskell, Manisha R Shah, Margaret M Halloran, Peter J PolveriniAbstract:ENDOTHELIAL Adhesion Molecules facilitate the entry of leukocytes into inflamed tissues. This in turn promotes neovascularization, a process central to the progression of rheumatoid arthritis, tumour growth and wound repair1. Here we test the hypothesis that soluble endothelial Adhesion Molecules promote angiogenesis2a¤-4. Human recombinant soluble E-selectin and soluble vascular Cell Adhesion Molecule-1 induced chemotaxis of human endothelial Cells in vitro and were angiogenic in rat cornea. Soluble E-selectin acted on endothelial Cells in part through a sialyl Lewis-X-dependent mechanism, while soluble vascular Cell Adhesion Molecule-1 acted on endothelial Cells in part through a very late antigen (VLA)-4 dependent mechanism. The chemotactic activity of rheumatoid synovial fluid for endothelial Cells, and also its angiogenic activity, were blocked by antibodies to either soluble E-selectin or soluble vascular Cell Adhesion Molecule-1. These results suggest a novel function for soluble endothelial Adhesion Molecules as mediators of angiogenesis.