The Experts below are selected from a list of 10770 Experts worldwide ranked by ideXlab platform
Thomas N Denny - One of the best experts on this subject based on the ideXlab platform.
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quality assurance program for peripheral blood mononuclear Cell Cryopreservation
Clinical and Vaccine Immunology, 2007Co-Authors: Adriana Weinberg, Raul Louzao, Marisa Marcia Mussipinhata, Maria Leticia Santos Cruz, Jorge Pinto, Maria F Huff, Andrea C De Castro, Maria Cecilia Araripe Sucupira, Thomas N DennyAbstract:Seven Brazilian sites participating in the Pediatric AIDS Clinical Trials Group international Cryopreservation quality assurance pilot program cryopreserved and shipped peripheral blood mononuclear Cells (PBMC) to a central U.S. laboratory for analysis. Cell viability and recovery significantly increased over time. A wet-laboratory training session conducted at the central laboratory significantly improved the quality of the cryopreserved PBMC.
Adriana Weinberg - One of the best experts on this subject based on the ideXlab platform.
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quality assurance program for peripheral blood mononuclear Cell Cryopreservation
Clinical and Vaccine Immunology, 2007Co-Authors: Adriana Weinberg, Raul Louzao, Marisa Marcia Mussipinhata, Maria Leticia Santos Cruz, Jorge Pinto, Maria F Huff, Andrea C De Castro, Maria Cecilia Araripe Sucupira, Thomas N DennyAbstract:Seven Brazilian sites participating in the Pediatric AIDS Clinical Trials Group international Cryopreservation quality assurance pilot program cryopreserved and shipped peripheral blood mononuclear Cells (PBMC) to a central U.S. laboratory for analysis. Cell viability and recovery significantly increased over time. A wet-laboratory training session conducted at the central laboratory significantly improved the quality of the cryopreserved PBMC.
Gang Zhao - One of the best experts on this subject based on the ideXlab platform.
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predehydration and ice seeding in the presence of trehalose enable Cell Cryopreservation
ACS Biomaterials Science & Engineering, 2017Co-Authors: Haishui Huang, Gang Zhao, Yuntian Zhang, Jiangsheng Xu, Thomas L Toth, Xiaoming HeAbstract:Conventional approaches for Cell Cryopreservation require the use of toxic membrane-penetrating cryoprotective agents (pCPA), which limits the clinical application of cryopreserved Cells. Here, we show intentionally induced ice formation at a high subzero temperature (> −10 °C) during Cryopreservation, which is often referred to as ice seeding, could result in significant Cell injury in the absence of any pCPA. This issue can be mitigated by predehydrating Cells using extraCellular trehalose to their minimal volume with minimized osmotically active water before ice seeding. We further observe that ice seeding can minimize the interfacial free energy that drives the devastating ice recrystallization-induced Cell injury during warming cryopreserved samples. Indeed, by combining predehydration using extraCellular trehalose with ice seeding at high subzero temperatures, high Cell viability or recovery is achieved for fibroblasts, adult stem Cells, and red blood Cells after Cryopreservation without using any p...
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alginate hydrogel microencapsulation inhibits devitrification and enables large volume low cpa Cell vitrification
Advanced Functional Materials, 2015Co-Authors: Haishui Huang, Jung Kyu Choi, Wei Rao, Shuting Zhao, Pranay Agarwal, Gang ZhaoAbstract:Cryopreservation of stem Cells is important to meet their ever-increasing demand by the burgeoning Cell-based medicine. The conventional slow freezing for stem Cell Cryopreservation suffers from inevitable Cell injury associated with ice formation and the vitrification (i.e., no visible ice formation) approach is emerging as a new strategy for Cell Cryopreservation. A major challenge to Cell vitrification is intraCellular ice formation (IIF, a lethal event to Cells) induced by devitrification (i.e., formation of visible ice in previously vitrified solution) during warming the vitrified Cells at cryogenic temperature back to super-zero temperatures. Consequently, high and toxic concentrations of penetrating cryoprotectants (i.e., high CPAs, up to ~8 M) and/or limited sample volumes (up to ~2.5 μl) have been used to minimize IIF during vitrification. We reveal that alginate hydrogel microencapsulation can effectively inhibit devitrification during warming. Our data show that if ice formation were minimized during cooling, IIF is negligible in alginate hydrogel-microencapsulated Cells during the entire cooling and warming procedure of vitrification. This enables vitrification of pluripotent and multipotent stem Cells with up to ~4 times lower concentration of penetrating CPAs (up to 2 M, low CPA) in up to ~100 times larger sample volume (up to ~250 μl, large volume).
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Dual dependence of cryobiogical properties of Sf21 Cell membrane on the temperature and the concentration of the cryoprotectant.
PLOS ONE, 2013Co-Authors: Jianye Wang, Gang ZhaoAbstract:The Sf21 Cell line is extensively used for virus research and producing heterologous recombinant proteins. To develop optimal strategies for minimizing Cell injury due to intraCellular ice formation and excessive volume shrinkage during Cryopreservation, the fundamental transport properties including the osmotic inactive volume (Vb), the hydraulic conductivity (Lp), and the glycerol permeability (Ps) of Sf21 Cell membrane at 25, 15, 5 and −2°C were characterized using a micro-perfusion chamber. The effects of temperature on the hydraulic conductivity and the glycerol permeability of Sf21 Cell membrane, reflected by the activation energies, were quantitatively investigated. It was found that the hydraulic conductivity decreases along with the increase of the final CPA concentration at a given temperature, and quantitative analysis indicates that the hydraulic conductivity has a significant linear attenuation along with the increase of the concentration of glycerol. Therefore, we incorporate the concentration dependence of the hydraulic conductivity into the classic Arrhenius relationship by replacing the constant reference value of the hydraulic conductivity at the reference temperature with a function that is linearly dependent on the CPA concentration. Consequently, the prediction of the Arrhenius relationship is improved, and the novel Arrhenius relationship could be very important to the development of optimal strategies for Cell Cryopreservation.
S C Goldstein - One of the best experts on this subject based on the ideXlab platform.
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Has allogeneic stem Cell Cryopreservation been given the ‘cold shoulder’? An analysis of the pros and cons of using frozen versus fresh stem Cell products in allogeneic stem Cell transplantation
Bone Marrow Transplantation, 2006Co-Authors: N V Frey, H M Lazarus, S C GoldsteinAbstract:Donor stem Cells for allogeneic transplant traditionally are collected and transfused ‘fresh’ into the recipient on the day of transplant; alternatively such Cells can be collected in advance and cryopreserved until needed. Most centers favor the former approach based on theoretical concerns that Cryopreservation and thawing may worsen clinical outcomes. Limited published data from single institution retrospective studies show no significant impairment of engraftment or reduced day 100 survival for cryopreserved bone marrow recipients. There are no reported outcomes for recipients of cryopreserved peripheral blood allografts. Use of cryopreserved stem Cells is associated with a higher incidence of adverse events (transfusion reactions, bacterial graft contamination and collection of grafts which are not utilized). Conversely, use of cryopreserved grafts introduces a greater flexibility into a stressed healthcare system and results in a more streamlined experience for the donor. Some data suggest that transplantation with a cryopreserved product may lower the incidence of acute graft-versus-host disease. We compare the pros and cons of using ‘fresh’ versus cryopreserved stem Cell products for allogeneic transplantation and suggest that the current standard of using ‘fresh’ products may not be warranted. We also suggest future areas of exploration to better elucidate this issue.
F Van Der Veen - One of the best experts on this subject based on the ideXlab platform.
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parental desire and acceptability of spermatogonial stem Cell Cryopreservation in boys with cancer
Human Reproduction, 2007Co-Authors: H Van Den Berg, Sjoerd Repping, F Van Der VeenAbstract:BACKGROUND: In the near future, a substantial proportion of adults will be childhood cancer survivors. The Cryopreservation and transplantation of spermatogonial stem Cells (SSCs) is currently successful in animals; application in humans seems likely in the near future. Cryopreserving SSCs might become an important issue in childhood cancer. Because this might require testicular biopsies or hemicastration, parental desire/acceptability for SSC collection was enquired for. METHODS: Three hundred eighteen parents of boys surviving at least 2 years after the diagnosis of cancer were asked about collecting SSCs by biopsy or hemicastration and collecting sperm by masturbation or electrostimulation. Opinions were assessed as if at the time of diagnosis and at the present time. RESULTS: Sixty-three per cent of parents responded. At diagnosis, SSC collection by means of biopsy was approved by 61%, hemicastration by 33% and collecting sperm by 70% (P < 0.013). The acceptability of performing hemicastration was significantly lower than all other forms of SSC/sperm collection. No differences were observed between parents’ present opinion and opinion at diagnosis. No differences related to treatment intensity, presumed negative fertility effects and pubertal state were found. CONCLUSIONS: Infertility is a major topic for parents. For prepubertal boys, the collection of SSCs might be a great relief in respect of the fertility issue. Collecting SSCs by biopsy is desired and accepted by the majority of parents; hemicastration is accepted by one-third of parents. The translation of SSC Cryopreservation and transplantation from animal models to humans is eagerly awaited.