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Grover M. Hutchins - One of the best experts on this subject based on the ideXlab platform.
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henrietta lacks hela Cells and Cell Culture contamination
Archives of Pathology & Laboratory Medicine, 2009Co-Authors: Brendan P. Lucey, Walter A Nelsonrees, Grover M. HutchinsAbstract:Abstract Henrietta Lacks died in 1951 of an aggressive adenocarcinoma of the cervix. A tissue biopsy obtained for diagnostic evaluation yielded additional tissue for Dr George O. Gey's tissue Culture laboratory at Johns Hopkins (Baltimore, Maryland). The cancer Cells, now called HeLa Cells, grew rapidly in Cell Culture and became the first human Cell line. HeLa Cells were used by researchers around the world. However, 20 years after Henrietta Lacks' death, mounting evidence suggested that HeLa Cells contaminated and overgrew other Cell lines. Cultures, supposedly of tissues such as breast cancer or mouse, proved to be HeLa Cells. We describe the history behind the development of HeLa Cells, including the first published description of Ms Lacks' autopsy, and the Cell Culture contamination that resulted. The debate over Cell Culture contamination began in the 1970s and was not harmonious. Ultimately, the problem was not resolved and it continues today. Finally, we discuss the philosophical implications of t...
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henrietta lacks hela Cells and Cell Culture contamination
Archives of Pathology & Laboratory Medicine, 2009Co-Authors: Brendan P. Lucey, Walter A Nelsonrees, Grover M. HutchinsAbstract:Henrietta Lacks died in 1951 of an aggressive adenocarcinoma of the cervix. A tissue biopsy obtained for diagnostic evaluation yielded additional tissue for Dr George O. Gey's tissue Culture laboratory at Johns Hopkins (Baltimore, Maryland). The cancer Cells, now called HeLa Cells, grew rapidly in Cell Culture and became the first human Cell line. HeLa Cells were used by researchers around the world. However, 20 years after Henrietta Lacks' death, mounting evidence suggested that HeLa Cells contaminated and overgrew other Cell lines. Cultures, supposedly of tissues such as breast cancer or mouse, proved to be HeLa Cells. We describe the history behind the development of HeLa Cells, including the first published description of Ms Lacks' autopsy, and the Cell Culture contamination that resulted. The debate over Cell Culture contamination began in the 1970s and was not harmonious. Ultimately, the problem was not resolved and it continues today. Finally, we discuss the philosophical implications of the immortal HeLa Cell line.
Barry Halliwell - One of the best experts on this subject based on the ideXlab platform.
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artefacts in Cell Culture α ketoglutarate can scavenge hydrogen peroxide generated by ascorbate and epigallocatechin gallate in Cell Culture media
Biochemical and Biophysical Research Communications, 2011Co-Authors: Lee Hua Long, Barry HalliwellAbstract:Ascorbate and several phenolic compounds readily oxidise in Cell Culture media to generate hydrogen peroxide. However, addition of α-ketoglutarate, which is known to be released by several Cell types, decreased the levels of H(2)O(2), and the α-ketoglutarate was depleted and converted to succinate. These observations could account for previous reports of the protective effects of α-ketoglutarate in promoting the growth of Cells in Culture, and may contribute to explaining some of the variability in the literature in reported rates of H(2)O(2) production from autoxidisable compounds in Cell Culture systems.
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artefacts in Cell Culture pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in Cell Culture media
Biochemical and Biophysical Research Communications, 2009Co-Authors: Lee Hua Long, Barry HalliwellAbstract:Ascorbate and several phenolic compounds readily oxidise in Cell Culture media to generate hydrogen peroxide. However, media containing pyruvate showed much less H 2 O 2 production, apparently because pyruvate can scavenge H 2 O 2 in the medium. Researchers must be aware that compounds under test can sometimes readily oxidise in Cell Culture media, that this might not be detected by measurement of H 2 O 2 if the media contain pyruvate, and that pyruvate can be substantially depleted in the media as a result.
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oxidative stress in Cell Culture an under appreciated problem
FEBS Letters, 2003Co-Authors: Barry HalliwellAbstract:Cell Culture studies have given much valuable information about mechanisms of metabolism and signal transduction and of regulation of gene expression, proliferation, senescence, and death. However, Cells in Culture may behave differently from Cells in vivo in many ways. One of these is that Cell Culture imposes a state of oxidative stress on Cells. I argue that Cells that survive and grow in Culture might use ROS-dependent signal transduction pathways that rarely or never operate in vivo. A further problem is that Cell Culture media can catalyse the oxidation of compounds added to them, resulting in apparent Cellular effects that are in fact due to oxidation products such as ROS. Such artefacts may have affected many studies on the effects of ascorbate, thiols, flavonoids and other polyphenolic compounds on Cells in Culture.
Brendan P. Lucey - One of the best experts on this subject based on the ideXlab platform.
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henrietta lacks hela Cells and Cell Culture contamination
Archives of Pathology & Laboratory Medicine, 2009Co-Authors: Brendan P. Lucey, Walter A Nelsonrees, Grover M. HutchinsAbstract:Abstract Henrietta Lacks died in 1951 of an aggressive adenocarcinoma of the cervix. A tissue biopsy obtained for diagnostic evaluation yielded additional tissue for Dr George O. Gey's tissue Culture laboratory at Johns Hopkins (Baltimore, Maryland). The cancer Cells, now called HeLa Cells, grew rapidly in Cell Culture and became the first human Cell line. HeLa Cells were used by researchers around the world. However, 20 years after Henrietta Lacks' death, mounting evidence suggested that HeLa Cells contaminated and overgrew other Cell lines. Cultures, supposedly of tissues such as breast cancer or mouse, proved to be HeLa Cells. We describe the history behind the development of HeLa Cells, including the first published description of Ms Lacks' autopsy, and the Cell Culture contamination that resulted. The debate over Cell Culture contamination began in the 1970s and was not harmonious. Ultimately, the problem was not resolved and it continues today. Finally, we discuss the philosophical implications of t...
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henrietta lacks hela Cells and Cell Culture contamination
Archives of Pathology & Laboratory Medicine, 2009Co-Authors: Brendan P. Lucey, Walter A Nelsonrees, Grover M. HutchinsAbstract:Henrietta Lacks died in 1951 of an aggressive adenocarcinoma of the cervix. A tissue biopsy obtained for diagnostic evaluation yielded additional tissue for Dr George O. Gey's tissue Culture laboratory at Johns Hopkins (Baltimore, Maryland). The cancer Cells, now called HeLa Cells, grew rapidly in Cell Culture and became the first human Cell line. HeLa Cells were used by researchers around the world. However, 20 years after Henrietta Lacks' death, mounting evidence suggested that HeLa Cells contaminated and overgrew other Cell lines. Cultures, supposedly of tissues such as breast cancer or mouse, proved to be HeLa Cells. We describe the history behind the development of HeLa Cells, including the first published description of Ms Lacks' autopsy, and the Cell Culture contamination that resulted. The debate over Cell Culture contamination began in the 1970s and was not harmonious. Ultimately, the problem was not resolved and it continues today. Finally, we discuss the philosophical implications of the immortal HeLa Cell line.
Walter A Nelsonrees - One of the best experts on this subject based on the ideXlab platform.
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henrietta lacks hela Cells and Cell Culture contamination
Archives of Pathology & Laboratory Medicine, 2009Co-Authors: Brendan P. Lucey, Walter A Nelsonrees, Grover M. HutchinsAbstract:Abstract Henrietta Lacks died in 1951 of an aggressive adenocarcinoma of the cervix. A tissue biopsy obtained for diagnostic evaluation yielded additional tissue for Dr George O. Gey's tissue Culture laboratory at Johns Hopkins (Baltimore, Maryland). The cancer Cells, now called HeLa Cells, grew rapidly in Cell Culture and became the first human Cell line. HeLa Cells were used by researchers around the world. However, 20 years after Henrietta Lacks' death, mounting evidence suggested that HeLa Cells contaminated and overgrew other Cell lines. Cultures, supposedly of tissues such as breast cancer or mouse, proved to be HeLa Cells. We describe the history behind the development of HeLa Cells, including the first published description of Ms Lacks' autopsy, and the Cell Culture contamination that resulted. The debate over Cell Culture contamination began in the 1970s and was not harmonious. Ultimately, the problem was not resolved and it continues today. Finally, we discuss the philosophical implications of t...
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henrietta lacks hela Cells and Cell Culture contamination
Archives of Pathology & Laboratory Medicine, 2009Co-Authors: Brendan P. Lucey, Walter A Nelsonrees, Grover M. HutchinsAbstract:Henrietta Lacks died in 1951 of an aggressive adenocarcinoma of the cervix. A tissue biopsy obtained for diagnostic evaluation yielded additional tissue for Dr George O. Gey's tissue Culture laboratory at Johns Hopkins (Baltimore, Maryland). The cancer Cells, now called HeLa Cells, grew rapidly in Cell Culture and became the first human Cell line. HeLa Cells were used by researchers around the world. However, 20 years after Henrietta Lacks' death, mounting evidence suggested that HeLa Cells contaminated and overgrew other Cell lines. Cultures, supposedly of tissues such as breast cancer or mouse, proved to be HeLa Cells. We describe the history behind the development of HeLa Cells, including the first published description of Ms Lacks' autopsy, and the Cell Culture contamination that resulted. The debate over Cell Culture contamination began in the 1970s and was not harmonious. Ultimately, the problem was not resolved and it continues today. Finally, we discuss the philosophical implications of the immortal HeLa Cell line.
Spiros N. Agathos - One of the best experts on this subject based on the ideXlab platform.
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insect Cell Culture
Manual of industrial microbiology and biotechnology, 2010Co-Authors: Spiros N. AgathosAbstract:The insect Cell-baculovirus expression vector system (IC-BEVS) is a highly versatile system because it can express gene products of practically any origin (from bacteria to human tissue), and in contrast to most industrial mammalian Cell Culture systems, it is based on engineering only the vector and not the host Cell line. As a result, the development time needed to progress from gene cloning to protein overproduction is much shorter. Compared to other biomanufacturing platforms, the IC-BEVS offers other significant advantages, including typically high product titers, a range of posttranslational modifications, and the possibility to express multimeric proteins or even several distinct proteins using the same vector. The Sf lines are adapted to suspension cultivation and are easily detached from T-flask (or other recipient) surfaces by gentle agitation without trypsinization. Small bench-scale Culture involves volumes ranging from a total of a few milliliters in wells of multiple-well plates or T-flasks for adherent Culture to shake flasks or spinners up to 250 ml for suspension Culture. A number of bioreactor systems have been investigated and proven merits of bioreactors in terms of robustness and scalability. Batch Culture remains the most common method for large scale IC-BEVS processing because of its inherent simplicity and flexibility in bioreactor equipment. The practice of insect Cell Culture is becoming more and more widespread as an essential component of the IC-BEVS, perhaps one of the most successful and far-reaching expression systems available today for the production of recombinant proteins and viral particles.
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Production scale insect Cell Culture.
Biotechnology Advances, 1991Co-Authors: Spiros N. AgathosAbstract:Abstract Insect Cells in Culture are currently commanding great interest as superior hosts for the efficient production of biologicals with applications in health care and in agriCulture. Insect Cell Culture is ripe for scale-up technologies, in order to meet future projected production requirements of (a) insect viruses used as bioinsecticides and (b) recombinant proteins of therapeutic potential for humans and animals. The single most prominent system used in research-based and in commercial insect Cell Culture today involves lepidopteran Cells transfected with baculovirus expression vectors for abundant formation of recombinant biologicals. However, dipteran insect Cell lines also are beginning to emerge as useful tools in biotechnology. Current practices in bioprocess development using insect Cell Culture, advances in media formulation and in insect Cell bioreactor design, and emerging trends are presented and critically evaluated.