The Experts below are selected from a list of 115134 Experts worldwide ranked by ideXlab platform
Alicja Ziemienowicz - One of the best experts on this subject based on the ideXlab platform.
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proliferating Cell nuclear antigen pcna a key factor in dna replication and Cell Cycle Regulation
Annals of Botany, 2011Co-Authors: Wojciech Strzalka, Alicja ZiemienowiczAbstract:Background PCNA (proliferating Cell nuclear antigen) has been found in the nuclei of yeast, plant and animal Cells that undergo Cell division, suggesting a function in Cell Cycle Regulation and/or DNA replication. It subsequently became clear that PCNA also played a role in other processes involving the Cell genome.
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review part of a special issue on the plant Cell Cycle proliferating Cell nuclear antigen pcna a key factor in dna replication and Cell Cycle Regulation
2011Co-Authors: Wojciech Strzalka, Alicja ZiemienowiczAbstract:†Background PCNA (proliferating Cell nuclear antigen) has been found in the nuclei of yeast, plant and animal Cells that undergo Cell division, suggesting a function in Cell Cycle Regulation and/or DNA replication. It subsequently became clear that PCNA also played a role in other processes involving the Cell genome. †Scope Thisreviewdiscusses eukaryoticPCNA,withanemphasisonplantPCNA,intermsoftheproteinstructure and its biochemical properties as well as gene structure, organization, expression and function. PCNA exerts a tripartite function by operating as (1) a sliding clamp during DNA synthesis, (2) a polymerase switch factor and (3) a recruitment factor. Most of its functions are mediated by its interactions with various proteins involved in DNA synthesis, repair and recombination as well as in Regulation of the Cell Cycle and chromatid cohesion. Moreover, post-translational modifications of PCNA playa key role in Regulation of its functions. Finally, aphylogenetic comparison of PCNA genes suggests that the multi-functionality observed in most species is a product of evolution. †Conclusions Most plant PCNAs exhibit features similar to those found for PCNAs of other eukaryotes. Similarities include: (1) a trimeric ring structure of the PCNA sliding clamp, (2) the involvement of PCNA in DNA replication and repair, (3) the ability to stimulate the activity of DNA polymerase d and (4) the ability to interact with p21, a regulator of the Cell Cycle. However, many plant genomes seem to contain the second, probably functional, copy of the PCNA gene, in contrast to PCNA pseudogenes that are found in mammalian genomes.
Rodney P Dekoter - One of the best experts on this subject based on the ideXlab platform.
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a role for atp citrate lyase in Cell Cycle Regulation during myeloid differentiation
Blood Cells Molecules and Diseases, 2019Co-Authors: Jess Rhee, Lauren A Solomon, Rodney P DekoterAbstract:Abstract Differentiation of myeloid progenitor Cells into macrophages is accompanied by increased PU.1 concentration and increasing Cell Cycle length, culminating in Cell Cycle arrest. Induction of PU.1 expression in a cultured myeloid Cell line expressing low PU.1 concentration results in decreased levels of mRNA encoding ATP-Citrate Lyase (ACL) and Cell Cycle arrest. ACL is an essential enzyme for generating acetyl-CoA, a key metabolite for the first step in fatty acid synthesis and for histone acetylation. We hypothesized that ACL may play a role in Cell Cycle Regulation in the myeloid lineage. In this study, we found that acetyl-CoA or acetate supplementation was sufficient to rescue Cell Cycle progression in cultured BN Cells treated with an ACL inhibitor or induced for PU.1 expression. Acetyl-CoA supplementation was also sufficient to rescue Cell Cycle progression in BN Cells treated with a fatty acid synthase (FASN) inhibitor. We demonstrated that acetyl-CoA was utilized in both fatty acid synthesis and histone acetylation pathways to promote proliferation. Finally, we found that Acly mRNA transcript levels decrease during normal macrophage differentiation from bone marrow precursors. Our results suggest that Regulation of ACL activity is a potentially important point of control for Cell Cycle Regulation in the myeloid lineage.
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a role for atp citrate lyase in Cell Cycle Regulation during myeloid differentiation
bioRxiv, 2019Co-Authors: Jess Rhee, Lauren A Solomon, Rodney P DekoterAbstract:Differentiation of myeloid progenitor Cells into macrophages is accompanied by increased PU.1 concentration and increasing Cell Cycle length, culminating in Cell Cycle arrest. Induction of PU.1 expression in a cultured myeloid Cell line expressing low PU.1 concentration results in decreased levels of mRNA encoding ATP-Citrate Lyase (ACL) and Cell Cycle arrest. ACL is an essential enzyme for generating acetyl-CoA, a key metabolite for the first step in fatty acid synthesis as well as for histone acetylation. We hypothesized that ACL may play a role in Cell Cycle Regulation in the myeloid lineage. In this study, we found that acetyl-CoA or acetate supplementation was sufficient to rescue Cell Cycle progression in cultured BN Cells treated with an ACL inhibitor or induced for PU.1 expression. Acetyl-CoA supplementation was also sufficient to rescue Cell Cycle progression in BN Cells treated with a fatty acid synthase (FASN) inhibitor. We demonstrated that acetyl-CoA was utilized in both fatty acid synthesis and histone acetylation pathways to promote proliferation. Finally, we found that Acly mRNA transcript levels decrease during normal macrophage differentiation from bone marrow precursors. Our results suggest that Regulation of ACL activity is a potentially important point of control for Cell Cycle Regulation in the myeloid lineage.
Wojciech Strzalka - One of the best experts on this subject based on the ideXlab platform.
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proliferating Cell nuclear antigen pcna a key factor in dna replication and Cell Cycle Regulation
Annals of Botany, 2011Co-Authors: Wojciech Strzalka, Alicja ZiemienowiczAbstract:Background PCNA (proliferating Cell nuclear antigen) has been found in the nuclei of yeast, plant and animal Cells that undergo Cell division, suggesting a function in Cell Cycle Regulation and/or DNA replication. It subsequently became clear that PCNA also played a role in other processes involving the Cell genome.
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review part of a special issue on the plant Cell Cycle proliferating Cell nuclear antigen pcna a key factor in dna replication and Cell Cycle Regulation
2011Co-Authors: Wojciech Strzalka, Alicja ZiemienowiczAbstract:†Background PCNA (proliferating Cell nuclear antigen) has been found in the nuclei of yeast, plant and animal Cells that undergo Cell division, suggesting a function in Cell Cycle Regulation and/or DNA replication. It subsequently became clear that PCNA also played a role in other processes involving the Cell genome. †Scope Thisreviewdiscusses eukaryoticPCNA,withanemphasisonplantPCNA,intermsoftheproteinstructure and its biochemical properties as well as gene structure, organization, expression and function. PCNA exerts a tripartite function by operating as (1) a sliding clamp during DNA synthesis, (2) a polymerase switch factor and (3) a recruitment factor. Most of its functions are mediated by its interactions with various proteins involved in DNA synthesis, repair and recombination as well as in Regulation of the Cell Cycle and chromatid cohesion. Moreover, post-translational modifications of PCNA playa key role in Regulation of its functions. Finally, aphylogenetic comparison of PCNA genes suggests that the multi-functionality observed in most species is a product of evolution. †Conclusions Most plant PCNAs exhibit features similar to those found for PCNAs of other eukaryotes. Similarities include: (1) a trimeric ring structure of the PCNA sliding clamp, (2) the involvement of PCNA in DNA replication and repair, (3) the ability to stimulate the activity of DNA polymerase d and (4) the ability to interact with p21, a regulator of the Cell Cycle. However, many plant genomes seem to contain the second, probably functional, copy of the PCNA gene, in contrast to PCNA pseudogenes that are found in mammalian genomes.
Jess Rhee - One of the best experts on this subject based on the ideXlab platform.
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a role for atp citrate lyase in Cell Cycle Regulation during myeloid differentiation
Blood Cells Molecules and Diseases, 2019Co-Authors: Jess Rhee, Lauren A Solomon, Rodney P DekoterAbstract:Abstract Differentiation of myeloid progenitor Cells into macrophages is accompanied by increased PU.1 concentration and increasing Cell Cycle length, culminating in Cell Cycle arrest. Induction of PU.1 expression in a cultured myeloid Cell line expressing low PU.1 concentration results in decreased levels of mRNA encoding ATP-Citrate Lyase (ACL) and Cell Cycle arrest. ACL is an essential enzyme for generating acetyl-CoA, a key metabolite for the first step in fatty acid synthesis and for histone acetylation. We hypothesized that ACL may play a role in Cell Cycle Regulation in the myeloid lineage. In this study, we found that acetyl-CoA or acetate supplementation was sufficient to rescue Cell Cycle progression in cultured BN Cells treated with an ACL inhibitor or induced for PU.1 expression. Acetyl-CoA supplementation was also sufficient to rescue Cell Cycle progression in BN Cells treated with a fatty acid synthase (FASN) inhibitor. We demonstrated that acetyl-CoA was utilized in both fatty acid synthesis and histone acetylation pathways to promote proliferation. Finally, we found that Acly mRNA transcript levels decrease during normal macrophage differentiation from bone marrow precursors. Our results suggest that Regulation of ACL activity is a potentially important point of control for Cell Cycle Regulation in the myeloid lineage.
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a role for atp citrate lyase in Cell Cycle Regulation during myeloid differentiation
bioRxiv, 2019Co-Authors: Jess Rhee, Lauren A Solomon, Rodney P DekoterAbstract:Differentiation of myeloid progenitor Cells into macrophages is accompanied by increased PU.1 concentration and increasing Cell Cycle length, culminating in Cell Cycle arrest. Induction of PU.1 expression in a cultured myeloid Cell line expressing low PU.1 concentration results in decreased levels of mRNA encoding ATP-Citrate Lyase (ACL) and Cell Cycle arrest. ACL is an essential enzyme for generating acetyl-CoA, a key metabolite for the first step in fatty acid synthesis as well as for histone acetylation. We hypothesized that ACL may play a role in Cell Cycle Regulation in the myeloid lineage. In this study, we found that acetyl-CoA or acetate supplementation was sufficient to rescue Cell Cycle progression in cultured BN Cells treated with an ACL inhibitor or induced for PU.1 expression. Acetyl-CoA supplementation was also sufficient to rescue Cell Cycle progression in BN Cells treated with a fatty acid synthase (FASN) inhibitor. We demonstrated that acetyl-CoA was utilized in both fatty acid synthesis and histone acetylation pathways to promote proliferation. Finally, we found that Acly mRNA transcript levels decrease during normal macrophage differentiation from bone marrow precursors. Our results suggest that Regulation of ACL activity is a potentially important point of control for Cell Cycle Regulation in the myeloid lineage.
Dan S Ray - One of the best experts on this subject based on the ideXlab platform.
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identification of cis and trans elements involved in the Cell Cycle Regulation of multiple genes in crithidia fasciculata
Molecular and Cellular Biology, 1999Co-Authors: Riaz Mahmood, Jane C Hines, Dan S RayAbstract:Transcripts of several DNA replication genes, including the RPA1 and TOP2 genes, encoding the large subunit of nuclear replication protein A and the kinetoplast topoisomerase II, accumulate periodically during the Cell Cycle in the trypanosomatid Crithidia fasciculata. An octamer consensus sequence, CAUAGAAG, present in the 5′ untranslated regions (UTR) of these mRNAs is required for periodic accumulation of the TOP2 and RPA1 transcripts and also for binding of a nuclear factor(s) to the 5′ UTR RNAs of these genes. We show here that insertion of multiple (six) copies of this octamer sequence (6× octamer) into the 5′ UTR of a reporter gene confers periodic accumulation on its transcript. Competition experiments and UV cross-linking studies show that the 6× octamer RNA and TOP2 5′ UTR RNA bind to the same nuclear factor(s). Single-nucleotide substitutions in the 6× octamer that abolish the RNA gel shift also prevent cyclic accumulation of the reporter gene transcript. A protein termed cycling element binding protein, purified by affinity chromatography using 6× octamer RNA as a ligand, binds to RNAs containing wild-type octamers and not to those with mutant octamers. These results define a small sequence element in C. fasciculata mRNAs required for their Cell Cycle Regulation and report the identification and purification of a putative regulatory protein that binds specifically to these elements.
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nulear extracts of crithidia fasciculata contain a factor s that binds to the 5 untranslated regions of top2 and rpa1 mrnas containing sequences required for their Cell Cycle Regulation
Journal of Biological Chemistry, 1998Co-Authors: Riaz Mahmood, Dan S RayAbstract:The Crithidia fasciculata replication protein A gene, RPA1, and topoisomerase II gene,TOP2, encode proteins involved in the replication of nuclear and mitochondrial DNA, respectively. Transcripts of both genes accumulate periodically during the Cell Cycle and attain their maximum levels just before S phase. Octamer consensus sequences within the 5′-untranslated region (UTR) of both genes have been shown to be necessary for cycling of these transcripts. Using a gel retardation assay, we show here that nuclear extracts of C. fasciculatacontain a protein factor(s) that binds specifically to RNA from 5′-UTRs of TOP2 and RPA1 genes. In addition, mutations in the consensus octamer sequence abolish binding to the RNA in both cases. Ultraviolet cross-linking using a radiolabeled TOP25′-UTR probe identified proteins with apparent molecular masses of 74 and 37 kDa in the RNA-protein complex. Nuclear extracts prepared from synchronized Cells show that the binding activity varies during the Cell Cycle in parallel with TOP2 and RPA1mRNA levels. These results suggest that the Cell Cycle Regulation of the mRNA levels of trypanosomatid DNA replication genes may be mediated by binding of specific proteins to conserved sequences in the 5′-UTR of their transcripts.