The Experts below are selected from a list of 100227 Experts worldwide ranked by ideXlab platform
Eva Syková - One of the best experts on this subject based on the ideXlab platform.
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poly l lysine modified iron oxide nanoparticles for stem Cell Labeling
Bioconjugate Chemistry, 2008Co-Authors: Michal Babic, Pavla Jendelova, Kateřina Glogarova, Milan Hájek, Daniel Horák, Petr Lesný, Vít Herynek, Miroslava Trchova, Eva SykováAbstract:New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide and oxidation of the resulting magnetite with sodium hypochlorite, followed by the addition of poly(l-lysine) (PLL) solution. PLL of several molecular weights ranging from 146 (l-lysine) to 579 000 was tested as a coating to boost the intraCellular uptake of the nanoparticles. The nanoparticles were characterized by TEM, dynamic light scattering, FTIR, and ultrasonic spectrometry. TEM revealed that the particles were ca. 6 nm in diameter, while FTIR showed that their surfaces were well-coated with PLL. The interaction of PLL-modified iron oxide nanoparticles with DMEM culture medium was verified by UV–vis spectroscopy. Rat bone marrow stromal Cells (rMSCs) and human mesenchymal stem Cells (hMSC) were labeled with PLL-modified iron oxide nanoparticles or with Endorem (control). Optical microscopy and TEM confirmed the presence of PLL-modified iron oxide nanoparticles inside the ...
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d mannose modified iron oxide nanoparticles for stem Cell Labeling
Bioconjugate Chemistry, 2007Co-Authors: Daniel Horák, Pavla Jendelova, Milan Hájek, Michal Babic, Vít Herynek, Miroslava Trchova, Zbynek Pientka, Emil Pollert, Eva SykováAbstract:New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide according to two methods. In the first method, precipitation was done in the presence of D-mannose solution (in situ coating); the second method involved oxidation of precipitated magnetite with sodium hypochlorite followed by addition of D-mannose solution (postsynthesis coating). Selected nanoparticles were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), elemental analysis, dynamic light scattering, infrared (IR), X-ray powder analysis, and ultrasonic spectrometry. While the first preparation method produced very fine nanoparticles ca. 2 nm in diameter, the second one yielded ca. 6 nm particles. Addition of D-mannose after synthesis did not affect the iron oxide particle size. UV-vis spectroscopy suggested that D-mannose suppresses the nonspecific sorption of serum proteins from DMEM culture medium on magnetic nanoparticles. Rat bone marrow stromal Cells (rMSCs) were labeled with uncoated and d-mannose-modified iron oxide nanoparticles and with Endorem (Guerbet, France; control). Optical and transmission electron microscopy confirmed the presence of D-mannose-modified iron oxide nanoparticles inside the Cells. D-mannose-modified nanoparticles crossed the Cell membranes and were internalized well by the Cells. Relaxivity measurements of labeled Cells in gelatin revealed very high relaxivities only for postsynthesis D-mannose-coated iron oxide nanoparticles.
Michal Babic - One of the best experts on this subject based on the ideXlab platform.
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poly l lysine modified iron oxide nanoparticles for stem Cell Labeling
Bioconjugate Chemistry, 2008Co-Authors: Michal Babic, Pavla Jendelova, Kateřina Glogarova, Milan Hájek, Daniel Horák, Petr Lesný, Vít Herynek, Miroslava Trchova, Eva SykováAbstract:New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide and oxidation of the resulting magnetite with sodium hypochlorite, followed by the addition of poly(l-lysine) (PLL) solution. PLL of several molecular weights ranging from 146 (l-lysine) to 579 000 was tested as a coating to boost the intraCellular uptake of the nanoparticles. The nanoparticles were characterized by TEM, dynamic light scattering, FTIR, and ultrasonic spectrometry. TEM revealed that the particles were ca. 6 nm in diameter, while FTIR showed that their surfaces were well-coated with PLL. The interaction of PLL-modified iron oxide nanoparticles with DMEM culture medium was verified by UV–vis spectroscopy. Rat bone marrow stromal Cells (rMSCs) and human mesenchymal stem Cells (hMSC) were labeled with PLL-modified iron oxide nanoparticles or with Endorem (control). Optical microscopy and TEM confirmed the presence of PLL-modified iron oxide nanoparticles inside the ...
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d mannose modified iron oxide nanoparticles for stem Cell Labeling
Bioconjugate Chemistry, 2007Co-Authors: Daniel Horák, Pavla Jendelova, Milan Hájek, Michal Babic, Vít Herynek, Miroslava Trchova, Zbynek Pientka, Emil Pollert, Eva SykováAbstract:New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide according to two methods. In the first method, precipitation was done in the presence of D-mannose solution (in situ coating); the second method involved oxidation of precipitated magnetite with sodium hypochlorite followed by addition of D-mannose solution (postsynthesis coating). Selected nanoparticles were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), elemental analysis, dynamic light scattering, infrared (IR), X-ray powder analysis, and ultrasonic spectrometry. While the first preparation method produced very fine nanoparticles ca. 2 nm in diameter, the second one yielded ca. 6 nm particles. Addition of D-mannose after synthesis did not affect the iron oxide particle size. UV-vis spectroscopy suggested that D-mannose suppresses the nonspecific sorption of serum proteins from DMEM culture medium on magnetic nanoparticles. Rat bone marrow stromal Cells (rMSCs) were labeled with uncoated and d-mannose-modified iron oxide nanoparticles and with Endorem (Guerbet, France; control). Optical and transmission electron microscopy confirmed the presence of D-mannose-modified iron oxide nanoparticles inside the Cells. D-mannose-modified nanoparticles crossed the Cell membranes and were internalized well by the Cells. Relaxivity measurements of labeled Cells in gelatin revealed very high relaxivities only for postsynthesis D-mannose-coated iron oxide nanoparticles.
Daniel Horák - One of the best experts on this subject based on the ideXlab platform.
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poly l lysine modified iron oxide nanoparticles for stem Cell Labeling
Bioconjugate Chemistry, 2008Co-Authors: Michal Babic, Pavla Jendelova, Kateřina Glogarova, Milan Hájek, Daniel Horák, Petr Lesný, Vít Herynek, Miroslava Trchova, Eva SykováAbstract:New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide and oxidation of the resulting magnetite with sodium hypochlorite, followed by the addition of poly(l-lysine) (PLL) solution. PLL of several molecular weights ranging from 146 (l-lysine) to 579 000 was tested as a coating to boost the intraCellular uptake of the nanoparticles. The nanoparticles were characterized by TEM, dynamic light scattering, FTIR, and ultrasonic spectrometry. TEM revealed that the particles were ca. 6 nm in diameter, while FTIR showed that their surfaces were well-coated with PLL. The interaction of PLL-modified iron oxide nanoparticles with DMEM culture medium was verified by UV–vis spectroscopy. Rat bone marrow stromal Cells (rMSCs) and human mesenchymal stem Cells (hMSC) were labeled with PLL-modified iron oxide nanoparticles or with Endorem (control). Optical microscopy and TEM confirmed the presence of PLL-modified iron oxide nanoparticles inside the ...
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d mannose modified iron oxide nanoparticles for stem Cell Labeling
Bioconjugate Chemistry, 2007Co-Authors: Daniel Horák, Pavla Jendelova, Milan Hájek, Michal Babic, Vít Herynek, Miroslava Trchova, Zbynek Pientka, Emil Pollert, Eva SykováAbstract:New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide according to two methods. In the first method, precipitation was done in the presence of D-mannose solution (in situ coating); the second method involved oxidation of precipitated magnetite with sodium hypochlorite followed by addition of D-mannose solution (postsynthesis coating). Selected nanoparticles were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), elemental analysis, dynamic light scattering, infrared (IR), X-ray powder analysis, and ultrasonic spectrometry. While the first preparation method produced very fine nanoparticles ca. 2 nm in diameter, the second one yielded ca. 6 nm particles. Addition of D-mannose after synthesis did not affect the iron oxide particle size. UV-vis spectroscopy suggested that D-mannose suppresses the nonspecific sorption of serum proteins from DMEM culture medium on magnetic nanoparticles. Rat bone marrow stromal Cells (rMSCs) were labeled with uncoated and d-mannose-modified iron oxide nanoparticles and with Endorem (Guerbet, France; control). Optical and transmission electron microscopy confirmed the presence of D-mannose-modified iron oxide nanoparticles inside the Cells. D-mannose-modified nanoparticles crossed the Cell membranes and were internalized well by the Cells. Relaxivity measurements of labeled Cells in gelatin revealed very high relaxivities only for postsynthesis D-mannose-coated iron oxide nanoparticles.
Pavla Jendelova - One of the best experts on this subject based on the ideXlab platform.
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poly l lysine modified iron oxide nanoparticles for stem Cell Labeling
Bioconjugate Chemistry, 2008Co-Authors: Michal Babic, Pavla Jendelova, Kateřina Glogarova, Milan Hájek, Daniel Horák, Petr Lesný, Vít Herynek, Miroslava Trchova, Eva SykováAbstract:New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide and oxidation of the resulting magnetite with sodium hypochlorite, followed by the addition of poly(l-lysine) (PLL) solution. PLL of several molecular weights ranging from 146 (l-lysine) to 579 000 was tested as a coating to boost the intraCellular uptake of the nanoparticles. The nanoparticles were characterized by TEM, dynamic light scattering, FTIR, and ultrasonic spectrometry. TEM revealed that the particles were ca. 6 nm in diameter, while FTIR showed that their surfaces were well-coated with PLL. The interaction of PLL-modified iron oxide nanoparticles with DMEM culture medium was verified by UV–vis spectroscopy. Rat bone marrow stromal Cells (rMSCs) and human mesenchymal stem Cells (hMSC) were labeled with PLL-modified iron oxide nanoparticles or with Endorem (control). Optical microscopy and TEM confirmed the presence of PLL-modified iron oxide nanoparticles inside the ...
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d mannose modified iron oxide nanoparticles for stem Cell Labeling
Bioconjugate Chemistry, 2007Co-Authors: Daniel Horák, Pavla Jendelova, Milan Hájek, Michal Babic, Vít Herynek, Miroslava Trchova, Zbynek Pientka, Emil Pollert, Eva SykováAbstract:New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide according to two methods. In the first method, precipitation was done in the presence of D-mannose solution (in situ coating); the second method involved oxidation of precipitated magnetite with sodium hypochlorite followed by addition of D-mannose solution (postsynthesis coating). Selected nanoparticles were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), elemental analysis, dynamic light scattering, infrared (IR), X-ray powder analysis, and ultrasonic spectrometry. While the first preparation method produced very fine nanoparticles ca. 2 nm in diameter, the second one yielded ca. 6 nm particles. Addition of D-mannose after synthesis did not affect the iron oxide particle size. UV-vis spectroscopy suggested that D-mannose suppresses the nonspecific sorption of serum proteins from DMEM culture medium on magnetic nanoparticles. Rat bone marrow stromal Cells (rMSCs) were labeled with uncoated and d-mannose-modified iron oxide nanoparticles and with Endorem (Guerbet, France; control). Optical and transmission electron microscopy confirmed the presence of D-mannose-modified iron oxide nanoparticles inside the Cells. D-mannose-modified nanoparticles crossed the Cell membranes and were internalized well by the Cells. Relaxivity measurements of labeled Cells in gelatin revealed very high relaxivities only for postsynthesis D-mannose-coated iron oxide nanoparticles.
Miroslava Trchova - One of the best experts on this subject based on the ideXlab platform.
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poly l lysine modified iron oxide nanoparticles for stem Cell Labeling
Bioconjugate Chemistry, 2008Co-Authors: Michal Babic, Pavla Jendelova, Kateřina Glogarova, Milan Hájek, Daniel Horák, Petr Lesný, Vít Herynek, Miroslava Trchova, Eva SykováAbstract:New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide and oxidation of the resulting magnetite with sodium hypochlorite, followed by the addition of poly(l-lysine) (PLL) solution. PLL of several molecular weights ranging from 146 (l-lysine) to 579 000 was tested as a coating to boost the intraCellular uptake of the nanoparticles. The nanoparticles were characterized by TEM, dynamic light scattering, FTIR, and ultrasonic spectrometry. TEM revealed that the particles were ca. 6 nm in diameter, while FTIR showed that their surfaces were well-coated with PLL. The interaction of PLL-modified iron oxide nanoparticles with DMEM culture medium was verified by UV–vis spectroscopy. Rat bone marrow stromal Cells (rMSCs) and human mesenchymal stem Cells (hMSC) were labeled with PLL-modified iron oxide nanoparticles or with Endorem (control). Optical microscopy and TEM confirmed the presence of PLL-modified iron oxide nanoparticles inside the ...
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d mannose modified iron oxide nanoparticles for stem Cell Labeling
Bioconjugate Chemistry, 2007Co-Authors: Daniel Horák, Pavla Jendelova, Milan Hájek, Michal Babic, Vít Herynek, Miroslava Trchova, Zbynek Pientka, Emil Pollert, Eva SykováAbstract:New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide according to two methods. In the first method, precipitation was done in the presence of D-mannose solution (in situ coating); the second method involved oxidation of precipitated magnetite with sodium hypochlorite followed by addition of D-mannose solution (postsynthesis coating). Selected nanoparticles were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), elemental analysis, dynamic light scattering, infrared (IR), X-ray powder analysis, and ultrasonic spectrometry. While the first preparation method produced very fine nanoparticles ca. 2 nm in diameter, the second one yielded ca. 6 nm particles. Addition of D-mannose after synthesis did not affect the iron oxide particle size. UV-vis spectroscopy suggested that D-mannose suppresses the nonspecific sorption of serum proteins from DMEM culture medium on magnetic nanoparticles. Rat bone marrow stromal Cells (rMSCs) were labeled with uncoated and d-mannose-modified iron oxide nanoparticles and with Endorem (Guerbet, France; control). Optical and transmission electron microscopy confirmed the presence of D-mannose-modified iron oxide nanoparticles inside the Cells. D-mannose-modified nanoparticles crossed the Cell membranes and were internalized well by the Cells. Relaxivity measurements of labeled Cells in gelatin revealed very high relaxivities only for postsynthesis D-mannose-coated iron oxide nanoparticles.