The Experts below are selected from a list of 822657 Experts worldwide ranked by ideXlab platform
Weifang Yu - One of the best experts on this subject based on the ideXlab platform.
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application value of cytof 2 mass cytometer technology at single Cell Level in human gastric cancer Cells
Experimental Cell Research, 2019Co-Authors: Xia Jiang, Hong Zhang, Chao Li, Zengren Zhao, Weifang YuAbstract:Abstract Chemotherapy and radiotherapy are main adjuvant therapies for the treatment of gastric cancer, the treatment effects are individual difference, but the specific mechanism is unknown. CyTOF 2 mass cytometer (CyTOF) enables the detecting up to 135 parameters on single Cell, the emergence of which is an opportunity for proteomics research. We first tried to apply CyTOF technique to gastric cancer Cells. We verified applicability of CyTOF in gastric cancer Cells, and analyzed the responses of seventeen proteins to chemoradiotherapy in human gastric cancer AGS Cells. To analyze the high dimensional CyTOF data, we used two statistical and visualization tools including viSNE and Citrus. Two specific clusters were found which had differences in protein expression profiles. CyTOF technology is proved feasibility and value at single Cell Level of gastric cancer.
Yasuo Chikusa - One of the best experts on this subject based on the ideXlab platform.
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all solid state lithium sulfur battery with high energy and power densities at the Cell Level
Energy technology, 2016Co-Authors: Hiroshi Nagata, Yasuo ChikusaAbstract:The performance of an all-solid-state lithium–sulfur battery, comprised of a positive composite electrode with high P/S ratio (number of phosphorus atoms/number of sulfur atoms) and solid electrolyte, is investigated at 25 °C at several loading weights of the positive electrode. The activation energy is nearly independent of the loading weight of the positive composite electrode. This suggests that influence of the sulfur reactivity is significantly larger than those of the ionic and electric conductivities. This positive composite electrode provides a capacity of above 1550 mAh g−1 (sulfur) at 1.3 mA cm−2 and above 6600 W kg−1 (sulfur) at 50 % state-of-charge for a loading weight of 8.3 mg cm−2. Moreover, in using this positive electrode, the energy and power densities are estimated to be 520 Wh kg−1 (970 Wh L−1) and 1230 W kg−1 (2300 W L−1), respectively, at the Cell Level (18650). Furthermore, the cycling performance of the Cell with this composite positive electrode is exCellent over several hundred cycles.
Xia Jiang - One of the best experts on this subject based on the ideXlab platform.
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application value of cytof 2 mass cytometer technology at single Cell Level in human gastric cancer Cells
Experimental Cell Research, 2019Co-Authors: Xia Jiang, Hong Zhang, Chao Li, Zengren Zhao, Weifang YuAbstract:Abstract Chemotherapy and radiotherapy are main adjuvant therapies for the treatment of gastric cancer, the treatment effects are individual difference, but the specific mechanism is unknown. CyTOF 2 mass cytometer (CyTOF) enables the detecting up to 135 parameters on single Cell, the emergence of which is an opportunity for proteomics research. We first tried to apply CyTOF technique to gastric cancer Cells. We verified applicability of CyTOF in gastric cancer Cells, and analyzed the responses of seventeen proteins to chemoradiotherapy in human gastric cancer AGS Cells. To analyze the high dimensional CyTOF data, we used two statistical and visualization tools including viSNE and Citrus. Two specific clusters were found which had differences in protein expression profiles. CyTOF technology is proved feasibility and value at single Cell Level of gastric cancer.
M Brice - One of the best experts on this subject based on the ideXlab platform.
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resolving the fibrotic niche of human liver cirrhosis at single Cell Level
Nature, 2019Co-Authors: Prakash Ramachandran, Ross Dobie, John R Wilsonkanamori, Elena Dora, Beth E P Henderson, N T Luu, Jordan R Portman, Kylie P Matchett, M BriceAbstract:Liver cirrhosis is a major cause of death worldwide and is characterized by extensive fibrosis. There are currently no effective antifibrotic therapies available. To obtain a better understanding of the Cellular and molecular mechanisms involved in disease pathogenesis and enable the discovery of therapeutic targets, here we profile the transcriptomes of more than 100,000 single human Cells, yielding molecular definitions for non-parenchymal Cell types that are found in healthy and cirrhotic human liver. We identify a scar-associated TREM2+CD9+ subpopulation of macrophages, which expands in liver fibrosis, differentiates from circulating monocytes and is pro-fibrogenic. We also define ACKR1+ and PLVAP+ endothelial Cells that expand in cirrhosis, are topographically restricted to the fibrotic niche and enhance the transmigration of leucocytes. Multi-lineage modelling of ligand and receptor interactions between the scar-associated macrophages, endothelial Cells and PDGFRα+ collagen-producing mesenchymal Cells reveals intra-scar activity of several pro-fibrogenic pathways including TNFRSF12A, PDGFR and NOTCH signalling. Our work dissects unanticipated aspects of the Cellular and molecular basis of human organ fibrosis at a single-Cell Level, and provides a conceptual framework for the discovery of rational therapeutic targets in liver cirrhosis.
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resolving the fibrotic niche of human liver cirrhosis at single Cell Level
Nature, 2019Co-Authors: Prakash Ramachandran, Ross Dobie, John R Wilsonkanamori, Elena Dora, Jordan R Portman, Kylie P Matchett, M Brice, Beth Henderson, John A Marwick, Richard S TaylorAbstract:Liver cirrhosis is a major cause of death worldwide and is characterized by extensive fibrosis. There are currently no effective antifibrotic therapies available. To obtain a better understanding of the Cellular and molecular mechanisms involved in disease pathogenesis and enable the discovery of therapeutic targets, here we profile the transcriptomes of more than 100,000 single human Cells, yielding molecular definitions for non-parenchymal Cell types that are found in healthy and cirrhotic human liver. We identify a scar-associated TREM2+CD9+ subpopulation of macrophages, which expands in liver fibrosis, differentiates from circulating monocytes and is pro-fibrogenic. We also define ACKR1+ and PLVAP+ endothelial Cells that expand in cirrhosis, are topographically restricted to the fibrotic niche and enhance the transmigration of leucocytes. Multi-lineage modelling of ligand and receptor interactions between the scar-associated macrophages, endothelial Cells and PDGFRα+ collagen-producing mesenchymal Cells reveals intra-scar activity of several pro-fibrogenic pathways including TNFRSF12A, PDGFR and NOTCH signalling. Our work dissects unanticipated aspects of the Cellular and molecular basis of human organ fibrosis at a single-Cell Level, and provides a conceptual framework for the discovery of rational therapeutic targets in liver cirrhosis. Single-Cell RNA sequencing is used to characterize and compare the functional diversity of Cells from liver biopsies of human scarred and normal liver, and identifies markers for scar-associated macrophages and endothelial Cells.
Prakash Ramachandran - One of the best experts on this subject based on the ideXlab platform.
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resolving the fibrotic niche of human liver cirrhosis at single Cell Level
Nature, 2019Co-Authors: Prakash Ramachandran, Ross Dobie, John R Wilsonkanamori, Elena Dora, Beth E P Henderson, N T Luu, Jordan R Portman, Kylie P Matchett, M BriceAbstract:Liver cirrhosis is a major cause of death worldwide and is characterized by extensive fibrosis. There are currently no effective antifibrotic therapies available. To obtain a better understanding of the Cellular and molecular mechanisms involved in disease pathogenesis and enable the discovery of therapeutic targets, here we profile the transcriptomes of more than 100,000 single human Cells, yielding molecular definitions for non-parenchymal Cell types that are found in healthy and cirrhotic human liver. We identify a scar-associated TREM2+CD9+ subpopulation of macrophages, which expands in liver fibrosis, differentiates from circulating monocytes and is pro-fibrogenic. We also define ACKR1+ and PLVAP+ endothelial Cells that expand in cirrhosis, are topographically restricted to the fibrotic niche and enhance the transmigration of leucocytes. Multi-lineage modelling of ligand and receptor interactions between the scar-associated macrophages, endothelial Cells and PDGFRα+ collagen-producing mesenchymal Cells reveals intra-scar activity of several pro-fibrogenic pathways including TNFRSF12A, PDGFR and NOTCH signalling. Our work dissects unanticipated aspects of the Cellular and molecular basis of human organ fibrosis at a single-Cell Level, and provides a conceptual framework for the discovery of rational therapeutic targets in liver cirrhosis.
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resolving the fibrotic niche of human liver cirrhosis at single Cell Level
Nature, 2019Co-Authors: Prakash Ramachandran, Ross Dobie, John R Wilsonkanamori, Elena Dora, Jordan R Portman, Kylie P Matchett, M Brice, Beth Henderson, John A Marwick, Richard S TaylorAbstract:Liver cirrhosis is a major cause of death worldwide and is characterized by extensive fibrosis. There are currently no effective antifibrotic therapies available. To obtain a better understanding of the Cellular and molecular mechanisms involved in disease pathogenesis and enable the discovery of therapeutic targets, here we profile the transcriptomes of more than 100,000 single human Cells, yielding molecular definitions for non-parenchymal Cell types that are found in healthy and cirrhotic human liver. We identify a scar-associated TREM2+CD9+ subpopulation of macrophages, which expands in liver fibrosis, differentiates from circulating monocytes and is pro-fibrogenic. We also define ACKR1+ and PLVAP+ endothelial Cells that expand in cirrhosis, are topographically restricted to the fibrotic niche and enhance the transmigration of leucocytes. Multi-lineage modelling of ligand and receptor interactions between the scar-associated macrophages, endothelial Cells and PDGFRα+ collagen-producing mesenchymal Cells reveals intra-scar activity of several pro-fibrogenic pathways including TNFRSF12A, PDGFR and NOTCH signalling. Our work dissects unanticipated aspects of the Cellular and molecular basis of human organ fibrosis at a single-Cell Level, and provides a conceptual framework for the discovery of rational therapeutic targets in liver cirrhosis. Single-Cell RNA sequencing is used to characterize and compare the functional diversity of Cells from liver biopsies of human scarred and normal liver, and identifies markers for scar-associated macrophages and endothelial Cells.