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Tandi E Matsha - One of the best experts on this subject based on the ideXlab platform.
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Peripheral Blood Mononuclear Cell Membrane Fluidity and Disease Outcome in Patients with Multiple Sclerosis
Indian Journal of Hematology and Blood Transfusion, 2012Co-Authors: Gloudina M Hon, Mogamat Shafick Hassan, Stefan Abel, Rajiv T Erasmus, Susan J. Rensburg, Tandi E MatshaAbstract:Immune Cell Membrane lipids are important determinants of Membrane Fluidity, eicosanoid production and phagocytosis and fatty acid metabolic abnormalities have been reported in immune Cells from patients with multiple sclerosis. The aim of this study was to investigate the relationship between peripheral blood mononuclear Cell Membrane Fluidity, permeability status, and disease outcome as measured by the Kurtzke expanded disability status scale. Phospholipids, fatty acids and cholesterol composition in peripheral blood mononuclear Cells from 26 patients diagnosed with multiple sclerosis and 25 healthy control subjects were determined by colorimetric assay, gas chromatography and enzymatic assays, respectively. Membrane Fluidity was calculated according to previously established formulae and correlated with C-reactive protein and the Kurtzke expanded disability status scale. There were no significant differences in Membrane lipids in peripheral blood mononuclear Cells from patients and controls. However, correlation studies showed lipid metabolic abnormalities, which were reflected in significant correlations between Membrane Fluidity as measured by both its fatty acid and phospholipid compositions, and the functional system scores. C-reactive protein showed positive correlations with phosphatidylcholine, phosphatidylserine, phosphatidylinositol and total phospholipids in Membranes from control subjects. Metabolic abnormalities, as well as correlations between Membrane Fluidity and the functional system scores, suggested the involvement of these immune Cell Membranes in the disease progression. Furthermore, the changed relationship between Membrane phospholipids and C-reactive protein, which has been shown to correlate with infectious episodes and clinical relapse, could be an indication of immune Cell dysfunction in patients with multiple sclerosis.
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red blood Cell Membrane Fluidity in the etiology of multiple sclerosis
The Journal of Membrane Biology, 2009Co-Authors: Gloudina M Hon, Mogamat Shafick Hassan, Susan J Van Rensburg, Stefan Abel, Paul J Van Jaarsveld, Rajiv T Erasmus, Tandi E MatshaAbstract:Organisms adjust the order, or Fluidity, of their Cellular Membranes in response to changes in their physiochemical environment by adjusting the lipid composition of their Membranes. We investigated Membrane Fluidity using the phospholipid, fatty acid and cholesterol content of red blood Cells (RBCs) from multiple sclerosis (MS) patients and correlated this with C-reactive protein (CRP) as well as with the severity of neurological outcome as measured by the Kurtzke Expanded Disability Status Scale (EDSS) and its Functional System Scores. The study group consisted of 31 patients with MS and 30 healthy control subjects. Phospholipids were determined using a colorimetric assay, fatty acids by gas chromatography, cholesterol by an enzymatic assay and CRP by a Beckman nephelometer. Cell Membrane Fluidity was calculated according to previously established formulae. RBC Membrane Fluidity as measured by the saturated to polyunsaturated fatty acid ratio was higher in patients than in controls (P = 0.04). The phosphatidylethanolamine saturated to polyunsaturated fatty acid ratio showed highly significant positive correlations with the EDSS and CRP < 5 μg/ml. CRP showed significant inverse correlations with the saturated nature but positive correlations with the ordered-crystalline-phase to liquid-crystalline-phase lipid ratio. In this study we show that Membrane Fluidity as measured by the relationship between Membrane fatty acids, phospholipids and cholesterol is closely interrelated with inflammation and disease outcome in patients with MS. In conclusion, our findings suggest that the Membrane lipid composition of patients with MS and, consequently, Membrane Fluidity are altered, which seems to be influenced by the inflammatory status.
P.j. Butler - One of the best experts on this subject based on the ideXlab platform.
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shear stress induces a time and position dependent increase in endothelial Cell Membrane Fluidity
American Journal of Physiology-cell Physiology, 2001Co-Authors: G. Norwich, Sheldon Weinbaum, P.j. Butler, Shu ChienAbstract:Blood flow-associated shear stress may modulate Cellular processes through its action on the plasma Membrane. We quantified the spatial and temporal aspects of the effects of shear stress (τ) on th...
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Shear stress increases endothelial Cell-Membrane Fluidity
Proceedings of the First Joint BMES EMBS Conference. 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Annual Fall Mee, 1999Co-Authors: P.j. Butler, G. Norwich, Sheldon Weinbaum, Shu ChienAbstract:Cell-Membrane Fluidity modulates protein function and mobility. Blood flow-associated shear stress induces changes in endothelial Cell functions, many of which may be initiated by alterations in the plasma Membrane. In this study, we quantify the effects of shear stress on endothelial Cell-Membrane lipid-Fluidity measured by fluorescence recovery after photobleaching (FRAP). A confocal microscope was used for FRAP-measurements on DiI-stained bovine aortic endothelial Cells in a parallel-plate flow chamber under static conditions, after a step-shear stress of 10 dynes/cm/sup 2/ which was maintained for 15 minutes, and after the removal of shear stress. The DiI-diffusion coefficient (D) increased immediately and significantly from a pre-step value of 4.3/spl plusmn/0.48/spl times/10/sup -9/ to 8.7/spl plusmn/1.8 /spl times/10/sup -9/ cm/sup 2//sec within 2 min after the initiation of shear stress and remained elevated thereafter. D returned to control values within 1 minute after cessation of shear stress. The novel measurements of shear-induced increases in Membrane-Fluidity provide important insight into the mechanisms of shear-induced protein modulation and mobilization.
Shu Chien - One of the best experts on this subject based on the ideXlab platform.
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shear stress induces a time and position dependent increase in endothelial Cell Membrane Fluidity
American Journal of Physiology-cell Physiology, 2001Co-Authors: G. Norwich, Sheldon Weinbaum, P.j. Butler, Shu ChienAbstract:Blood flow-associated shear stress may modulate Cellular processes through its action on the plasma Membrane. We quantified the spatial and temporal aspects of the effects of shear stress (τ) on th...
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Shear stress increases endothelial Cell-Membrane Fluidity
Proceedings of the First Joint BMES EMBS Conference. 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Annual Fall Mee, 1999Co-Authors: P.j. Butler, G. Norwich, Sheldon Weinbaum, Shu ChienAbstract:Cell-Membrane Fluidity modulates protein function and mobility. Blood flow-associated shear stress induces changes in endothelial Cell functions, many of which may be initiated by alterations in the plasma Membrane. In this study, we quantify the effects of shear stress on endothelial Cell-Membrane lipid-Fluidity measured by fluorescence recovery after photobleaching (FRAP). A confocal microscope was used for FRAP-measurements on DiI-stained bovine aortic endothelial Cells in a parallel-plate flow chamber under static conditions, after a step-shear stress of 10 dynes/cm/sup 2/ which was maintained for 15 minutes, and after the removal of shear stress. The DiI-diffusion coefficient (D) increased immediately and significantly from a pre-step value of 4.3/spl plusmn/0.48/spl times/10/sup -9/ to 8.7/spl plusmn/1.8 /spl times/10/sup -9/ cm/sup 2//sec within 2 min after the initiation of shear stress and remained elevated thereafter. D returned to control values within 1 minute after cessation of shear stress. The novel measurements of shear-induced increases in Membrane-Fluidity provide important insight into the mechanisms of shear-induced protein modulation and mobilization.
Jie Bai - One of the best experts on this subject based on the ideXlab platform.
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enhancing effect of borneol and muscone on geniposide transport across the human nasal epithelial Cell monolayer
PLOS ONE, 2014Co-Authors: Zhenzhen Chen, Xin Gong, Zhihui Yang, Jie BaiAbstract:Geniposide is widely used in the treatment of cerebral ischemic stroke and cerebrovascular diseases for its anti-thrombotic and anti-inflammatory effects. Recent studies demonstrated that geniposide could be absorbed promptly and thoroughly by intranasal administration in mice and basically transported into the brain. Here, we explored its transport mechanism and the effect of borneol and muscone on its transport by human nasal epithelial Cell (HNEC) monolayer. The cytotoxicity of geniposide, borneol, muscone and their combinations on HNECs was evaluated by the MTT assay. TransCellular transport of geniposide and the influence of borneol and muscone were studied using the HNEC monolayer. Immunostaining and transepithelial electrical resistance were measured to assess the integrity of the monolayer. The Membrane Fluidity of HNEC was evaluated by fluorescence recovery after photobleaching. Geniposide showed relatively poor absorption in the HNEC monolayer and it was not a P-gp substrate. Geniposide transport in both directions significantly increased when co-administrated with increasing concentrations of borneol and muscone. The enhancing effect of borneol and muscone on geniposide transport across the HNEC may be attributed to the significant enhancement on Cell Membrane Fluidity, disassembly effect on tight junction integrity and the process was reversible. These results indicated that intranasal administration has good potential to treat cerebrovascular diseases.
Gloudina M Hon - One of the best experts on this subject based on the ideXlab platform.
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Peripheral Blood Mononuclear Cell Membrane Fluidity and Disease Outcome in Patients with Multiple Sclerosis
Indian Journal of Hematology and Blood Transfusion, 2012Co-Authors: Gloudina M Hon, Mogamat Shafick Hassan, Stefan Abel, Rajiv T Erasmus, Susan J. Rensburg, Tandi E MatshaAbstract:Immune Cell Membrane lipids are important determinants of Membrane Fluidity, eicosanoid production and phagocytosis and fatty acid metabolic abnormalities have been reported in immune Cells from patients with multiple sclerosis. The aim of this study was to investigate the relationship between peripheral blood mononuclear Cell Membrane Fluidity, permeability status, and disease outcome as measured by the Kurtzke expanded disability status scale. Phospholipids, fatty acids and cholesterol composition in peripheral blood mononuclear Cells from 26 patients diagnosed with multiple sclerosis and 25 healthy control subjects were determined by colorimetric assay, gas chromatography and enzymatic assays, respectively. Membrane Fluidity was calculated according to previously established formulae and correlated with C-reactive protein and the Kurtzke expanded disability status scale. There were no significant differences in Membrane lipids in peripheral blood mononuclear Cells from patients and controls. However, correlation studies showed lipid metabolic abnormalities, which were reflected in significant correlations between Membrane Fluidity as measured by both its fatty acid and phospholipid compositions, and the functional system scores. C-reactive protein showed positive correlations with phosphatidylcholine, phosphatidylserine, phosphatidylinositol and total phospholipids in Membranes from control subjects. Metabolic abnormalities, as well as correlations between Membrane Fluidity and the functional system scores, suggested the involvement of these immune Cell Membranes in the disease progression. Furthermore, the changed relationship between Membrane phospholipids and C-reactive protein, which has been shown to correlate with infectious episodes and clinical relapse, could be an indication of immune Cell dysfunction in patients with multiple sclerosis.
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red blood Cell Membrane Fluidity in the etiology of multiple sclerosis
The Journal of Membrane Biology, 2009Co-Authors: Gloudina M Hon, Mogamat Shafick Hassan, Susan J Van Rensburg, Stefan Abel, Paul J Van Jaarsveld, Rajiv T Erasmus, Tandi E MatshaAbstract:Organisms adjust the order, or Fluidity, of their Cellular Membranes in response to changes in their physiochemical environment by adjusting the lipid composition of their Membranes. We investigated Membrane Fluidity using the phospholipid, fatty acid and cholesterol content of red blood Cells (RBCs) from multiple sclerosis (MS) patients and correlated this with C-reactive protein (CRP) as well as with the severity of neurological outcome as measured by the Kurtzke Expanded Disability Status Scale (EDSS) and its Functional System Scores. The study group consisted of 31 patients with MS and 30 healthy control subjects. Phospholipids were determined using a colorimetric assay, fatty acids by gas chromatography, cholesterol by an enzymatic assay and CRP by a Beckman nephelometer. Cell Membrane Fluidity was calculated according to previously established formulae. RBC Membrane Fluidity as measured by the saturated to polyunsaturated fatty acid ratio was higher in patients than in controls (P = 0.04). The phosphatidylethanolamine saturated to polyunsaturated fatty acid ratio showed highly significant positive correlations with the EDSS and CRP < 5 μg/ml. CRP showed significant inverse correlations with the saturated nature but positive correlations with the ordered-crystalline-phase to liquid-crystalline-phase lipid ratio. In this study we show that Membrane Fluidity as measured by the relationship between Membrane fatty acids, phospholipids and cholesterol is closely interrelated with inflammation and disease outcome in patients with MS. In conclusion, our findings suggest that the Membrane lipid composition of patients with MS and, consequently, Membrane Fluidity are altered, which seems to be influenced by the inflammatory status.