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Hervy E. Averette - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of Paclitaxel (Taxol), Cisplatin, and the Combination Paclitaxel-Cisplatin in Ovarian Cancer in Vitro with the ATP Cell Viability Assay
Gynecologic Oncology, 1994Co-Authors: Michael Untch, James P. Perras, Andrea Untch, Randolph D. Hightower, Ossi R Koechli, Bernd-uwe Sevin, Roberto Angioli, Hervy E. AveretteAbstract:Abstract This study evaluates the in vitro sensitivities of 42 ovarian cancer specimens to the new anticancer agent Paclitaxel (taxol, Tx), cisplatin (DDP), and the combination Tx-DDP with the adenosine triphosphate Cell Viability Assay (ATP-CVA). In vitro response is defined by ⩾50% ATP decrease compared to untreated controls 6-7 days after drug treatment with 20% of the peak plasma concentration (PPC). Response rates were 12% to Tx, 19% to DDP, and 27% to Tx + DDP. The mean IC 50 's of Tx, DDP, and the combination Tx-DDP were (2.6×, 1.0×, and 0.38× PPC, respectively). The mean inhibition of Cell Viability was significantly greater with drug combinations compared to single drugs. In 7/11 tumors synergistic effects and in 2/11 additive effects were found between Tx and DDP. We conclude that based on ATP-CVA in vitro results, Tx-DDP shows significantly better activity compared to each of the single drugs in ovarian cancer.
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Comparative Chemosensitivity Profiles in Three Human Breast Cancer Cell Lines with the ATP-Cell Viability Assay
Oncology, 1994Co-Authors: Ossi R Koechli, James P. Perras, Albert Steren, Cheppail Ramachandran, Bernd-uwe Sevin, Roberto Angioli, Michael Untch, Hervy E. AveretteAbstract:In this study the dose-response curves for doxorubicin, pirarubicin, 5-fluoro-uracil, 4-hydroperoxy-cyclophosphamide and taxol were obtained in three breast cancer Cell lines (MCF-7, T47D and BT-20). The ATP Cell Viability Assay was chosen to evaluate the chemosensitivity profiles and was a reproducible, practicable method to assess drug response in breast cancer Cell lines. The IC50 values were calculated on the median effect principle and indicated that taxol was the most active drug tested in this study with a mean IC50 value of 0.02 μM. This in vitro effect correlated well with clinical observations in metastatic breast cancer where taxol proved to be a very active drug. Pirarubicin was the second most active drug tested with an IC50 value 10 times less compared to that of doxorubicin. The results obtained with the ATP Cell Viability Assay are promising, therefore further testing with drug combination chemotherapy and fresh human breast cancer tumor testing are warranted and ongoing.
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Growth Characteristics of Nonmalignant Cells in the ATP Cell Viability Assay
Oncology, 1994Co-Authors: Ossi R Koechli, James P. Perras, Albert Steren, Bernd-uwe Sevin, Roberto Angioli, Mike Rodriguez, Parvin Ganjei, Hervy E. AveretteAbstract:Over the last 5 years, the ATP Cell Viability Assay (ATP-CVA) has been used to study the in vitro response of Cell lines and fresh gynecologic human tumors to a variety of antineoplastic agents including chemotherapeutic agents, hormones and biological response modifiers. This Assay measures light production as intraCellular ATP interacts with the luciferin-luciferase complex. Quantitation of the light produced has been shown to directly correspond with the number of viable Cells. A past criticism is that in the ATP-CVA, when applied to fresh tumor tissue, normal Cells (fibroblasts, macrophages and lymphocytes) also produce ATP, and if present in sufficient numbers, could lead to errors in chemosensitivity testing results. This study was designed to evaluate the growth characteristics of various benign Cells found in fresh tumors. The Cells were studied under multiple plating conditions to show the relative increase or decrease of fractional ATP measured at different time points. We found that agar/McCoy underlayer and agarose-coated wells do not permit the growth of nonmalignant Cells. In the culture conditions of the ATP-CVA, non-malignant Cells do not contribute relevant ATP levels when treated samples are compared to controls on day 6. Therefore, results of the ATP-CVA in fresh tumors should not be affected.
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Comparison of paclitaxel and docetaxel (Taxotere) in gynecologic and breast cancer Cell lines with the ATP-Cell Viability Assay.
Anti-cancer drugs, 1994Co-Authors: Michael Untch, James P. Perras, Andrea Untch, Ossi R Koechli, Bernd-uwe Sevin, Roberto Angioli, Hervy E. AveretteAbstract:The in vitro effects of paclitaxel (Tx) and docetaxel (Taxotere, Txt) are compared in this study using the adenosine triphosphate Cell Viability Assay (ATP-CVA) in 14 cancer Cell lines. Eleven Cell lines were sensitive and three were partially sensitive to paclitaxel. Nine Cell lines were sensitive, three were partially sensitive and two were resistant to docetaxel. Mean IC50s were 3.7-660 ng/ml paclitaxel and 5.4-540 ng/ml docetaxel. In five sensitive cancer Cell lines docetaxel was more active than paclitaxel, and in six sensitive Cell lines paclitaxel was more active than docetaxel on a concentration basis. Two Cell lines were sensitive to paclitaxel and resistant to docetaxel. In one Cell line the two compounds had similar activities. In the ATP-CVA, paclitaxel and docetaxel are very active and are partially non-cross-resistant.
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Application of the adenosine triphosphate‐Cell Viability Assay in human breast cancer chemosensitivity testing: A report on the first results
Journal of Surgical Oncology, 1993Co-Authors: Ossi R Koechli, James P. Perras, Barry P. Avner, Bernd-uwe Sevin, David S. Robinson, Hervy E. AveretteAbstract:Chemosensitivity testing in vitro of breast cancer has been difficult because of small tumor volume, an even smaller yield of viable Cells after disaggregation, and the low evaluability rate and sensitivity of current Assays. We have employed an alternative approach that quantitates intraCellular adenosine triphosphate (ATP) as a measure of Cell Viability. This ATP-Cell Viability Assay (ATP-CVA) determines in vitro tumor Cell Viability after exposure to chemotherapeutic agents in comparison to untreated controls following 6 days of incubation. Sixty-one fresh breast cancer specimens upon testing yielded an evaluability rate of 95%. Forty-seven of the tumors were untreated primary breast cancers, the remaining 14 were from patients with metastatic disease. Correlations of in vitro drug sensitivity with in vivo response were obtained for 17 treatment regimens in 14 patients with metastatic breast cancer. The level of sensitivity was 90% and the specificity 86%. These preliminary data demonstrated the ATP-CVA to be a practical in vitro approach to breast cancer testing. It will require a larger clinical study for confirmation. © 1993 Wiley-Liss, Inc.
Rovshan Khalilov - One of the best experts on this subject based on the ideXlab platform.
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biosynthesis of agnps onto the urea based periodic mesoporous organosilica agxnps ur pmo for antibacterial and Cell Viability Assay
Journal of Colloid and Interface Science, 2021Co-Authors: Amir Hasanzadeh, Behnam Gholipour, Sadegh Rostamnia, Aziz Eftekhari, Asghar Tanomand, Ali Valizadeh. K, Samad Khaksar, Rovshan KhalilovAbstract:Abstract Nano-size silver particles were stabilized on the inner surfaces of urea based periodic mesoporous organosilica (Ur-PMO). Aqueous extract of Euphorbia leaves as a sustainable and green reducing agent was applied for Ag-nanoparticles growth into the Ur-PMO channels. Physical and chemical properties of organosilica materials synthesized using various techniques such as FT-IR, small-angle XRD, PXRD, FESEM, TEM, SEM-EDX and atomic absorption spectrometry (AAS) were examined. Finally, the AgNPs/Ur-PMO were investigated on Cell Viability Assay. An in vitro cytotoxicity test using MMT Assay displayed that the designed material has good biocompatibility and could be a promising candidate for biomedical applications. The results also showed that the AgNPs/Ur-PMO compounds (especially, PMO; 1.27% AgNPs) had relatively good antibacterial and antibiofilm effects. It seems that the use of these compounds in hospital environments can reduce nosocomial infections as well as reduce antibiotic-resistant bacteria.
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Biosynthesis of AgNPs onto the urea-based periodic mesoporous organosilica (AgxNPs/Ur-PMO) for antibacterial and Cell Viability Assay.
Journal of colloid and interface science, 2020Co-Authors: Amir Hasanzadeh, Behnam Gholipour, Sadegh Rostamnia, Aziz Eftekhari, Asghar Tanomand, Ali Valizadeh. K, Samad Khaksar, Rovshan KhalilovAbstract:Abstract Nano-size silver particles were stabilized on the inner surfaces of urea based periodic mesoporous organosilica (Ur-PMO). Aqueous extract of Euphorbia leaves as a sustainable and green reducing agent was applied for Ag-nanoparticles growth into the Ur-PMO channels. Physical and chemical properties of organosilica materials synthesized using various techniques such as FT-IR, small-angle XRD, PXRD, FESEM, TEM, SEM-EDX and atomic absorption spectrometry (AAS) were examined. Finally, the AgNPs/Ur-PMO were investigated on Cell Viability Assay. An in vitro cytotoxicity test using MMT Assay displayed that the designed material has good biocompatibility and could be a promising candidate for biomedical applications. The results also showed that the AgNPs/Ur-PMO compounds (especially, PMO; 1.27% AgNPs) had relatively good antibacterial and antibiofilm effects. It seems that the use of these compounds in hospital environments can reduce nosocomial infections as well as reduce antibiotic-resistant bacteria.
Ossi R Koechli - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of Paclitaxel (Taxol), Cisplatin, and the Combination Paclitaxel-Cisplatin in Ovarian Cancer in Vitro with the ATP Cell Viability Assay
Gynecologic Oncology, 1994Co-Authors: Michael Untch, James P. Perras, Andrea Untch, Randolph D. Hightower, Ossi R Koechli, Bernd-uwe Sevin, Roberto Angioli, Hervy E. AveretteAbstract:Abstract This study evaluates the in vitro sensitivities of 42 ovarian cancer specimens to the new anticancer agent Paclitaxel (taxol, Tx), cisplatin (DDP), and the combination Tx-DDP with the adenosine triphosphate Cell Viability Assay (ATP-CVA). In vitro response is defined by ⩾50% ATP decrease compared to untreated controls 6-7 days after drug treatment with 20% of the peak plasma concentration (PPC). Response rates were 12% to Tx, 19% to DDP, and 27% to Tx + DDP. The mean IC 50 's of Tx, DDP, and the combination Tx-DDP were (2.6×, 1.0×, and 0.38× PPC, respectively). The mean inhibition of Cell Viability was significantly greater with drug combinations compared to single drugs. In 7/11 tumors synergistic effects and in 2/11 additive effects were found between Tx and DDP. We conclude that based on ATP-CVA in vitro results, Tx-DDP shows significantly better activity compared to each of the single drugs in ovarian cancer.
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Comparative Chemosensitivity Profiles in Three Human Breast Cancer Cell Lines with the ATP-Cell Viability Assay
Oncology, 1994Co-Authors: Ossi R Koechli, James P. Perras, Albert Steren, Cheppail Ramachandran, Bernd-uwe Sevin, Roberto Angioli, Michael Untch, Hervy E. AveretteAbstract:In this study the dose-response curves for doxorubicin, pirarubicin, 5-fluoro-uracil, 4-hydroperoxy-cyclophosphamide and taxol were obtained in three breast cancer Cell lines (MCF-7, T47D and BT-20). The ATP Cell Viability Assay was chosen to evaluate the chemosensitivity profiles and was a reproducible, practicable method to assess drug response in breast cancer Cell lines. The IC50 values were calculated on the median effect principle and indicated that taxol was the most active drug tested in this study with a mean IC50 value of 0.02 μM. This in vitro effect correlated well with clinical observations in metastatic breast cancer where taxol proved to be a very active drug. Pirarubicin was the second most active drug tested with an IC50 value 10 times less compared to that of doxorubicin. The results obtained with the ATP Cell Viability Assay are promising, therefore further testing with drug combination chemotherapy and fresh human breast cancer tumor testing are warranted and ongoing.
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Growth Characteristics of Nonmalignant Cells in the ATP Cell Viability Assay
Oncology, 1994Co-Authors: Ossi R Koechli, James P. Perras, Albert Steren, Bernd-uwe Sevin, Roberto Angioli, Mike Rodriguez, Parvin Ganjei, Hervy E. AveretteAbstract:Over the last 5 years, the ATP Cell Viability Assay (ATP-CVA) has been used to study the in vitro response of Cell lines and fresh gynecologic human tumors to a variety of antineoplastic agents including chemotherapeutic agents, hormones and biological response modifiers. This Assay measures light production as intraCellular ATP interacts with the luciferin-luciferase complex. Quantitation of the light produced has been shown to directly correspond with the number of viable Cells. A past criticism is that in the ATP-CVA, when applied to fresh tumor tissue, normal Cells (fibroblasts, macrophages and lymphocytes) also produce ATP, and if present in sufficient numbers, could lead to errors in chemosensitivity testing results. This study was designed to evaluate the growth characteristics of various benign Cells found in fresh tumors. The Cells were studied under multiple plating conditions to show the relative increase or decrease of fractional ATP measured at different time points. We found that agar/McCoy underlayer and agarose-coated wells do not permit the growth of nonmalignant Cells. In the culture conditions of the ATP-CVA, non-malignant Cells do not contribute relevant ATP levels when treated samples are compared to controls on day 6. Therefore, results of the ATP-CVA in fresh tumors should not be affected.
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Comparison of paclitaxel and docetaxel (Taxotere) in gynecologic and breast cancer Cell lines with the ATP-Cell Viability Assay.
Anti-cancer drugs, 1994Co-Authors: Michael Untch, James P. Perras, Andrea Untch, Ossi R Koechli, Bernd-uwe Sevin, Roberto Angioli, Hervy E. AveretteAbstract:The in vitro effects of paclitaxel (Tx) and docetaxel (Taxotere, Txt) are compared in this study using the adenosine triphosphate Cell Viability Assay (ATP-CVA) in 14 cancer Cell lines. Eleven Cell lines were sensitive and three were partially sensitive to paclitaxel. Nine Cell lines were sensitive, three were partially sensitive and two were resistant to docetaxel. Mean IC50s were 3.7-660 ng/ml paclitaxel and 5.4-540 ng/ml docetaxel. In five sensitive cancer Cell lines docetaxel was more active than paclitaxel, and in six sensitive Cell lines paclitaxel was more active than docetaxel on a concentration basis. Two Cell lines were sensitive to paclitaxel and resistant to docetaxel. In one Cell line the two compounds had similar activities. In the ATP-CVA, paclitaxel and docetaxel are very active and are partially non-cross-resistant.
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Application of the adenosine triphosphate‐Cell Viability Assay in human breast cancer chemosensitivity testing: A report on the first results
Journal of Surgical Oncology, 1993Co-Authors: Ossi R Koechli, James P. Perras, Barry P. Avner, Bernd-uwe Sevin, David S. Robinson, Hervy E. AveretteAbstract:Chemosensitivity testing in vitro of breast cancer has been difficult because of small tumor volume, an even smaller yield of viable Cells after disaggregation, and the low evaluability rate and sensitivity of current Assays. We have employed an alternative approach that quantitates intraCellular adenosine triphosphate (ATP) as a measure of Cell Viability. This ATP-Cell Viability Assay (ATP-CVA) determines in vitro tumor Cell Viability after exposure to chemotherapeutic agents in comparison to untreated controls following 6 days of incubation. Sixty-one fresh breast cancer specimens upon testing yielded an evaluability rate of 95%. Forty-seven of the tumors were untreated primary breast cancers, the remaining 14 were from patients with metastatic disease. Correlations of in vitro drug sensitivity with in vivo response were obtained for 17 treatment regimens in 14 patients with metastatic breast cancer. The level of sensitivity was 90% and the specificity 86%. These preliminary data demonstrated the ATP-CVA to be a practical in vitro approach to breast cancer testing. It will require a larger clinical study for confirmation. © 1993 Wiley-Liss, Inc.
Sadegh Rostamnia - One of the best experts on this subject based on the ideXlab platform.
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biosynthesis of agnps onto the urea based periodic mesoporous organosilica agxnps ur pmo for antibacterial and Cell Viability Assay
Journal of Colloid and Interface Science, 2021Co-Authors: Amir Hasanzadeh, Behnam Gholipour, Sadegh Rostamnia, Aziz Eftekhari, Asghar Tanomand, Ali Valizadeh. K, Samad Khaksar, Rovshan KhalilovAbstract:Abstract Nano-size silver particles were stabilized on the inner surfaces of urea based periodic mesoporous organosilica (Ur-PMO). Aqueous extract of Euphorbia leaves as a sustainable and green reducing agent was applied for Ag-nanoparticles growth into the Ur-PMO channels. Physical and chemical properties of organosilica materials synthesized using various techniques such as FT-IR, small-angle XRD, PXRD, FESEM, TEM, SEM-EDX and atomic absorption spectrometry (AAS) were examined. Finally, the AgNPs/Ur-PMO were investigated on Cell Viability Assay. An in vitro cytotoxicity test using MMT Assay displayed that the designed material has good biocompatibility and could be a promising candidate for biomedical applications. The results also showed that the AgNPs/Ur-PMO compounds (especially, PMO; 1.27% AgNPs) had relatively good antibacterial and antibiofilm effects. It seems that the use of these compounds in hospital environments can reduce nosocomial infections as well as reduce antibiotic-resistant bacteria.
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Biosynthesis of AgNPs onto the urea-based periodic mesoporous organosilica (AgxNPs/Ur-PMO) for antibacterial and Cell Viability Assay.
Journal of colloid and interface science, 2020Co-Authors: Amir Hasanzadeh, Behnam Gholipour, Sadegh Rostamnia, Aziz Eftekhari, Asghar Tanomand, Ali Valizadeh. K, Samad Khaksar, Rovshan KhalilovAbstract:Abstract Nano-size silver particles were stabilized on the inner surfaces of urea based periodic mesoporous organosilica (Ur-PMO). Aqueous extract of Euphorbia leaves as a sustainable and green reducing agent was applied for Ag-nanoparticles growth into the Ur-PMO channels. Physical and chemical properties of organosilica materials synthesized using various techniques such as FT-IR, small-angle XRD, PXRD, FESEM, TEM, SEM-EDX and atomic absorption spectrometry (AAS) were examined. Finally, the AgNPs/Ur-PMO were investigated on Cell Viability Assay. An in vitro cytotoxicity test using MMT Assay displayed that the designed material has good biocompatibility and could be a promising candidate for biomedical applications. The results also showed that the AgNPs/Ur-PMO compounds (especially, PMO; 1.27% AgNPs) had relatively good antibacterial and antibiofilm effects. It seems that the use of these compounds in hospital environments can reduce nosocomial infections as well as reduce antibiotic-resistant bacteria.
Amir Hasanzadeh - One of the best experts on this subject based on the ideXlab platform.
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biosynthesis of agnps onto the urea based periodic mesoporous organosilica agxnps ur pmo for antibacterial and Cell Viability Assay
Journal of Colloid and Interface Science, 2021Co-Authors: Amir Hasanzadeh, Behnam Gholipour, Sadegh Rostamnia, Aziz Eftekhari, Asghar Tanomand, Ali Valizadeh. K, Samad Khaksar, Rovshan KhalilovAbstract:Abstract Nano-size silver particles were stabilized on the inner surfaces of urea based periodic mesoporous organosilica (Ur-PMO). Aqueous extract of Euphorbia leaves as a sustainable and green reducing agent was applied for Ag-nanoparticles growth into the Ur-PMO channels. Physical and chemical properties of organosilica materials synthesized using various techniques such as FT-IR, small-angle XRD, PXRD, FESEM, TEM, SEM-EDX and atomic absorption spectrometry (AAS) were examined. Finally, the AgNPs/Ur-PMO were investigated on Cell Viability Assay. An in vitro cytotoxicity test using MMT Assay displayed that the designed material has good biocompatibility and could be a promising candidate for biomedical applications. The results also showed that the AgNPs/Ur-PMO compounds (especially, PMO; 1.27% AgNPs) had relatively good antibacterial and antibiofilm effects. It seems that the use of these compounds in hospital environments can reduce nosocomial infections as well as reduce antibiotic-resistant bacteria.
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Biosynthesis of AgNPs onto the urea-based periodic mesoporous organosilica (AgxNPs/Ur-PMO) for antibacterial and Cell Viability Assay.
Journal of colloid and interface science, 2020Co-Authors: Amir Hasanzadeh, Behnam Gholipour, Sadegh Rostamnia, Aziz Eftekhari, Asghar Tanomand, Ali Valizadeh. K, Samad Khaksar, Rovshan KhalilovAbstract:Abstract Nano-size silver particles were stabilized on the inner surfaces of urea based periodic mesoporous organosilica (Ur-PMO). Aqueous extract of Euphorbia leaves as a sustainable and green reducing agent was applied for Ag-nanoparticles growth into the Ur-PMO channels. Physical and chemical properties of organosilica materials synthesized using various techniques such as FT-IR, small-angle XRD, PXRD, FESEM, TEM, SEM-EDX and atomic absorption spectrometry (AAS) were examined. Finally, the AgNPs/Ur-PMO were investigated on Cell Viability Assay. An in vitro cytotoxicity test using MMT Assay displayed that the designed material has good biocompatibility and could be a promising candidate for biomedical applications. The results also showed that the AgNPs/Ur-PMO compounds (especially, PMO; 1.27% AgNPs) had relatively good antibacterial and antibiofilm effects. It seems that the use of these compounds in hospital environments can reduce nosocomial infections as well as reduce antibiotic-resistant bacteria.