The Experts below are selected from a list of 25989 Experts worldwide ranked by ideXlab platform
Fiona S. L. Brinkman - One of the best experts on this subject based on the ideXlab platform.
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methods for predicting bacterial protein subCellular localization
Nature Reviews Microbiology, 2006Co-Authors: Jennifer L. Gardy, Fiona S. L. BrinkmanAbstract:The computational prediction of the particular Cellular Compartment that a bacterial protein is destined for is an important aspect of microbiological research. This article discusses the methods currently available to predict bacterial protein localization. The computational prediction of the subCellular localization of bacterial proteins is an important step in genome annotation and in the search for novel vaccine or drug targets. Since the 1991 release of PSORT I ? the first comprehensive algorithm to predict bacterial protein localization ? many other localization prediction tools have been developed. These methods offer significant improvements in predictive performance over PSORT I and the accuracy of some methods now rivals that of certain high-throughput laboratory methods for protein localization identification.
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Methods for predicting bacterial protein subCellular localization
Nature reviews. Microbiology, 2006Co-Authors: Jennifer L. Gardy, Fiona S. L. BrinkmanAbstract:The computational prediction of the particular Cellular Compartment that a bacterial protein is destined for is an important aspect of microbiological research. This article discusses the methods currently available to predict bacterial protein localization.
Abigail Savietto - One of the best experts on this subject based on the ideXlab platform.
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flotillin mediated membrane fluidity controls peptidoglycan synthesis and mreb movement
eLife, 2020Co-Authors: Aleksandra Zielinska, Abigail Savietto, Anabela De Sousa Borges, Denis Martinez, Melanie Berbon, Joel R Roelofsen, Alwin M Hartman, Rinse De BoerAbstract:The bacterial plasma membrane is an important Cellular Compartment. In recent years it has become obvious that protein complexes and lipids are not uniformly distributed within membranes. Current hypotheses suggest that flotillin proteins are required for the formation of complexes of membrane proteins including cell-wall synthetic proteins. We show here that bacterial flotillins are important factors for membrane fluidity homeostasis. Loss of flotillins leads to a decrease in membrane fluidity that in turn leads to alterations in MreB dynamics and, as a consequence, in peptidoglycan synthesis. These alterations are reverted when membrane fluidity is restored by a chemical fluidizer. In vitro, the addition of a flotillin increases membrane fluidity of liposomes. Our data support a model in which flotillins are required for direct control of membrane fluidity rather than for the formation of protein complexes via direct protein-protein interactions.
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membrane fluidity controls peptidoglycan synthesis and mreb movement
bioRxiv, 2019Co-Authors: Aleksandra Zielinska, Abigail Savietto, Anabela De Sousa Borges, Joel R Roelofsen, Alwin M Hartman, Rinse De Boer, Ida J Van Der Klei, Anna K H HirschAbstract:Abstract The bacterial plasma membrane is an important Cellular Compartment. In recent years it has become obvious that protein complexes and lipids are not uniformly distributed within membranes. Current hypotheses suggest that flotillin proteins are required for the formation of complexes of membrane proteins including cell-wall synthetic proteins. We show here that bacterial flotillins are important factors for membrane fluidity homeostasis. Loss of flotillins leads to changes in membrane fluidity that in turn lead to alterations in MreB dynamics and, as a consequence, in peptidoglycan synthesis. Our data support a model in which flotillins are required for growth-rate dependent control of membrane fluidity rather than for the formation of protein complexes via direct protein-protein interactions.
Alwin M Hartman - One of the best experts on this subject based on the ideXlab platform.
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flotillin mediated membrane fluidity controls peptidoglycan synthesis and mreb movement
eLife, 2020Co-Authors: Aleksandra Zielinska, Abigail Savietto, Anabela De Sousa Borges, Denis Martinez, Melanie Berbon, Joel R Roelofsen, Alwin M Hartman, Rinse De BoerAbstract:The bacterial plasma membrane is an important Cellular Compartment. In recent years it has become obvious that protein complexes and lipids are not uniformly distributed within membranes. Current hypotheses suggest that flotillin proteins are required for the formation of complexes of membrane proteins including cell-wall synthetic proteins. We show here that bacterial flotillins are important factors for membrane fluidity homeostasis. Loss of flotillins leads to a decrease in membrane fluidity that in turn leads to alterations in MreB dynamics and, as a consequence, in peptidoglycan synthesis. These alterations are reverted when membrane fluidity is restored by a chemical fluidizer. In vitro, the addition of a flotillin increases membrane fluidity of liposomes. Our data support a model in which flotillins are required for direct control of membrane fluidity rather than for the formation of protein complexes via direct protein-protein interactions.
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membrane fluidity controls peptidoglycan synthesis and mreb movement
bioRxiv, 2019Co-Authors: Aleksandra Zielinska, Abigail Savietto, Anabela De Sousa Borges, Joel R Roelofsen, Alwin M Hartman, Rinse De Boer, Ida J Van Der Klei, Anna K H HirschAbstract:Abstract The bacterial plasma membrane is an important Cellular Compartment. In recent years it has become obvious that protein complexes and lipids are not uniformly distributed within membranes. Current hypotheses suggest that flotillin proteins are required for the formation of complexes of membrane proteins including cell-wall synthetic proteins. We show here that bacterial flotillins are important factors for membrane fluidity homeostasis. Loss of flotillins leads to changes in membrane fluidity that in turn lead to alterations in MreB dynamics and, as a consequence, in peptidoglycan synthesis. Our data support a model in which flotillins are required for growth-rate dependent control of membrane fluidity rather than for the formation of protein complexes via direct protein-protein interactions.
Rinse De Boer - One of the best experts on this subject based on the ideXlab platform.
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flotillin mediated membrane fluidity controls peptidoglycan synthesis and mreb movement
eLife, 2020Co-Authors: Aleksandra Zielinska, Abigail Savietto, Anabela De Sousa Borges, Denis Martinez, Melanie Berbon, Joel R Roelofsen, Alwin M Hartman, Rinse De BoerAbstract:The bacterial plasma membrane is an important Cellular Compartment. In recent years it has become obvious that protein complexes and lipids are not uniformly distributed within membranes. Current hypotheses suggest that flotillin proteins are required for the formation of complexes of membrane proteins including cell-wall synthetic proteins. We show here that bacterial flotillins are important factors for membrane fluidity homeostasis. Loss of flotillins leads to a decrease in membrane fluidity that in turn leads to alterations in MreB dynamics and, as a consequence, in peptidoglycan synthesis. These alterations are reverted when membrane fluidity is restored by a chemical fluidizer. In vitro, the addition of a flotillin increases membrane fluidity of liposomes. Our data support a model in which flotillins are required for direct control of membrane fluidity rather than for the formation of protein complexes via direct protein-protein interactions.
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membrane fluidity controls peptidoglycan synthesis and mreb movement
bioRxiv, 2019Co-Authors: Aleksandra Zielinska, Abigail Savietto, Anabela De Sousa Borges, Joel R Roelofsen, Alwin M Hartman, Rinse De Boer, Ida J Van Der Klei, Anna K H HirschAbstract:Abstract The bacterial plasma membrane is an important Cellular Compartment. In recent years it has become obvious that protein complexes and lipids are not uniformly distributed within membranes. Current hypotheses suggest that flotillin proteins are required for the formation of complexes of membrane proteins including cell-wall synthetic proteins. We show here that bacterial flotillins are important factors for membrane fluidity homeostasis. Loss of flotillins leads to changes in membrane fluidity that in turn lead to alterations in MreB dynamics and, as a consequence, in peptidoglycan synthesis. Our data support a model in which flotillins are required for growth-rate dependent control of membrane fluidity rather than for the formation of protein complexes via direct protein-protein interactions.
Jennifer L. Gardy - One of the best experts on this subject based on the ideXlab platform.
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methods for predicting bacterial protein subCellular localization
Nature Reviews Microbiology, 2006Co-Authors: Jennifer L. Gardy, Fiona S. L. BrinkmanAbstract:The computational prediction of the particular Cellular Compartment that a bacterial protein is destined for is an important aspect of microbiological research. This article discusses the methods currently available to predict bacterial protein localization. The computational prediction of the subCellular localization of bacterial proteins is an important step in genome annotation and in the search for novel vaccine or drug targets. Since the 1991 release of PSORT I ? the first comprehensive algorithm to predict bacterial protein localization ? many other localization prediction tools have been developed. These methods offer significant improvements in predictive performance over PSORT I and the accuracy of some methods now rivals that of certain high-throughput laboratory methods for protein localization identification.
-
Methods for predicting bacterial protein subCellular localization
Nature reviews. Microbiology, 2006Co-Authors: Jennifer L. Gardy, Fiona S. L. BrinkmanAbstract:The computational prediction of the particular Cellular Compartment that a bacterial protein is destined for is an important aspect of microbiological research. This article discusses the methods currently available to predict bacterial protein localization.