The Experts below are selected from a list of 5622 Experts worldwide ranked by ideXlab platform

G R Welch - One of the best experts on this subject based on the ideXlab platform.

Ali Khademhosseini - One of the best experts on this subject based on the ideXlab platform.

  • Nanoscale and microscale approaches for engineering the in vitro Cellular Microenvironment
    2020
    Co-Authors: Ali Khademhosseini
    Abstract:

    Thesis (Ph. D.)--Massachusetts Institute of Technology, Biological Engineering Division, 2005.

  • hydrogels and microtechnologies for engineering the Cellular Microenvironment
    Wiley Interdisciplinary Reviews-nanomedicine and Nanobiotechnology, 2012
    Co-Authors: Robert Gauvin, Remi Parenteaubareil, Mehmet R Dokmeci, David W Merryman, Ali Khademhosseini
    Abstract:

    Hydrogels represent a class of materials suitable for numerous biomedical applications such as tissue engineering and drug delivery. Hydrogels are by definition capable of absorbing large amount of fluid, making them adequate for cell seeding and encapsulation as well as for implantation because of their biocompatibility and excellent diffusion properties. They also possess other desirable properties for fundamental research as they have the ability to mimic the basic three-dimensional (3D) biological, chemical, and mechanical properties of native tissues. Furthermore, their biological interactions with cells can be modified through the numerous side groups of the polymeric chains. Thus, the biological, chemical, and mechanical properties, as well as the degradation kinetics of hydrogels can be tailored depending on the application. In addition, their fabrication process can be combined with microtechnologies to enable precise control of cell-scale features such as surface topography and the presence of adhesion motifs on the hydrogel material. This ability to control the microscale structure of hydrogels has been used to engineer tissue models and to study cell behavior mechanisms in vitro. New approaches such as bottom-up and directed assembly of microscale hydrogels (microgels) are currently emerging as powerful methods to enable the fabrication of 3D constructs replicating the Microenvironment found in vivo. WIREs Nanomed Nanobiotechnol 2012, 4:235–246. doi: 10.1002/wnan.171 For further resources related to this article, please visit the WIREs website.

  • microscale technologies for tissue engineering and biology
    Proceedings of the National Academy of Sciences of the United States of America, 2006
    Co-Authors: Ali Khademhosseini, Robert Langer, Jeffrey T Borenstein, Joseph P Vacanti
    Abstract:

    Microscale technologies are emerging as powerful tools for tissue engineering and biological studies. In this review, we present an overview of these technologies in various tissue engineering applications, such as for fabricating 3D microfabricated scaffolds, as templates for cell aggregate formation, or for fabricating materials in a spatially regulated manner. In addition, we give examples of the use of microscale technologies for controlling the Cellular Microenvironment in vitro and for performing high-throughput assays. The use of microfluidics, surface patterning, and patterned cocultures in regulating various aspects of Cellular Microenvironment is discussed, as well as the application of these technologies in directing cell fate and elucidating the underlying biology. Throughout this review, we will use specific examples where available and will provide trends and future directions in the field.

Dmitry Volodkin - One of the best experts on this subject based on the ideXlab platform.

  • Hybrids of Polymer Multilayers, Lipids, and Nanoparticles: Mimicking the Cellular Microenvironment
    Langmuir, 2019
    Co-Authors: Anna S. Vikulina, Andre G. Skirtach, Dmitry Volodkin
    Abstract:

    Here we address research directions and trends developed following novel concepts in 2D/3D self-assembled polymer structures established in the department led by Helmuth Mohwald. These functional structures made of hybrids of polymer multilayers, lipids, and nanoparticles stimulated research in the design of the Cellular Microenvironment. The composition of the extraCellular matrix (ECM) and dynamics of biofactor presentation in the ECM can be recapitulated by the hybrids. Proteins serve as models for protein-based biofactors such as growth factors, cytokines, hormones, and so forth. A fundamental understanding of complex intermolecular interactions and approaches developed for the externally IR-light-triggered release offers a powerful tool for controlling the biofactor presentation. Pure protein beads made via a mild templating on vaterite CaCO3 crystals can mimic Cellular organelles in terms of the compartmentalization of active proteins. We believe that an integration of the approaches developed and d...

  • hybrids of polymer multilayers lipids and nanoparticles mimicking the Cellular Microenvironment
    Langmuir, 2019
    Co-Authors: Anna S. Vikulina, Andre G. Skirtach, Dmitry Volodkin
    Abstract:

    Here we address research directions and trends developed following novel concepts in 2D/3D self-assembled polymer structures established in the department led by Helmuth Mohwald. These functional structures made of hybrids of polymer multilayers, lipids, and nanoparticles stimulated research in the design of the Cellular Microenvironment. The composition of the extraCellular matrix (ECM) and dynamics of biofactor presentation in the ECM can be recapitulated by the hybrids. Proteins serve as models for protein-based biofactors such as growth factors, cytokines, hormones, and so forth. A fundamental understanding of complex intermolecular interactions and approaches developed for the externally IR-light-triggered release offers a powerful tool for controlling the biofactor presentation. Pure protein beads made via a mild templating on vaterite CaCO3 crystals can mimic Cellular organelles in terms of the compartmentalization of active proteins. We believe that an integration of the approaches developed and described here offers a strong tool for engineering and mimicking both extra- and intraCellular Microenvironments.

Jordan J Green - One of the best experts on this subject based on the ideXlab platform.

  • biomolecule delivery to engineer the Cellular Microenvironment for regenerative medicine
    Annals of Biomedical Engineering, 2014
    Co-Authors: Corey J Bishop, Jordan J Green
    Abstract:

    To realize the potential of regenerative medicine, controlling the delivery of biomolecules in the Cellular Microenvironment is important as these factors control cell fate. Controlled delivery for tissue engineering and regenerative medicine often requires bioengineered materials and cells capable of spatiotemporal modulation of biomolecule release and presentation. This review discusses biomolecule delivery from the outside of the cell inwards through the delivery of soluble and insoluble biomolecules as well as from the inside of the cell outwards through gene transfer. Ex vivo and in vivo therapeutic strategies are discussed, as well as combination delivery of biomolecules, scaffolds, and cells. Various applications in regenerative medicine are highlighted including bone tissue engineering and wound healing.

E V Dyatlovitskaya - One of the best experts on this subject based on the ideXlab platform.

  • the sphingenine sphinganine ratio in sphingolipids of transplantable rat tumors depends on a transplantation organ
    Bioorganicheskaia khimiia, 2003
    Co-Authors: A G Kandyba, V A Koblyakov, E V Dyatlovitskaya
    Abstract:

    The content of sphingenine (sphingosine) and sphinganine was determined in the total pool of sphingomyelin and ceramide in the rat tumors transplanted subcutaneously and intrahepatically. The sphingenine/sphinganine ratio in the subcutaneously transplanted sarcoma M1 and cholangioCellular carcinoma RS1 was lower than that in the sphingolipids of the intrahepatically transplanted tumors. However, the sphingenine/sphinganine ratio in the subcutaneously transplanted rat hepatoma 27 was higher than in the intrahepatically transplanted hepatoma. These observations indicate that the sphingenine/sphinganine ratio in sphingolipids of tumors depends on the tumor type and its Cellular Microenvironment.

  • The Sphingenine/Sphinganine Ratio in Sphingolipids of Transplantable Rat Tumors Depends on a Transplantation Organ
    Bioorganicheskaia khimiia, 2003
    Co-Authors: A G Kandyba, V A Koblyakov, E V Dyatlovitskaya
    Abstract:

    The content of sphingenine (sphingosine) and sphinganine was determined in the total pool of sphingomyelin and ceramide in the rat tumors transplanted subcutaneously and intrahepatically. The sphingenine/sphinganine ratio in the subcutaneously transplanted sarcoma M1 and cholangioCellular carcinoma RS1 was lower than that in the sphingolipids of the intrahepatically transplanted tumors. However, the sphingenine/sphinganine ratio in the subcutaneously transplanted rat hepatoma 27 was higher than in the intrahepatically transplanted hepatoma. These observations indicate that the sphingenine/sphinganine ratio in sphingolipids of tumors depends on the tumor type and its Cellular Microenvironment.