The Experts below are selected from a list of 294 Experts worldwide ranked by ideXlab platform

Saad M Bhamla - One of the best experts on this subject based on the ideXlab platform.

  • a 3d printed hand powered Centrifuge for molecular biology
    PLOS Biology, 2019
    Co-Authors: Gaurav Byagathvalli, Aaron Pomerantz, Soham Sinha, Janet Standeven, Saad M Bhamla
    Abstract:

    The Centrifuge is an essential tool for many aspects of research and medical diagnostics. However, conventional Centrifuges are often inaccessible outside of standard laboratory settings, such as remote field sites, because they require a constant external power source and can be prohibitively costly in resource-limited settings and Science, technology, engineering, and mathematics (STEM)-focused programs. Here we present the 3D-Fuge, a 3D-printed hand-powered Centrifuge, as a novel alternative to standard benchtop Centrifuges. Based on the design principles of a paper-based Centrifuge, this 3D-printed instrument increases the volume capacity to 2 mL and can reach hand-powered centrifugation speeds up to 6,000 rpm. The 3D-Fuge devices presented here are capable of centrifugation of a wide variety of different solutions such as spinning down samples for biomarker applications and performing nucleotide extractions as part of a portable molecular lab setup. We introduce the design and proof-of-principle trials that demonstrate the utility of low-cost 3D-printed Centrifuges for use in remote field biology and educational settings.

  • a 3d printed hand powered Centrifuge for molecular biology
    bioRxiv, 2019
    Co-Authors: Gaurav Byagathvalli, Aaron Pomerantz, Soham Sinha, Janet Standeven, Saad M Bhamla
    Abstract:

    The Centrifuge is an essential tool for many aspects of research and medical diagnostics. However, conventional Centrifuges are often inaccessible outside of conventional laboratory settings, such as remote field sites, require a constant external power source, and can be prohibitively costly in resource-limited settings and STEM-focused programs. Here we present the 3D-Fuge, a 3D-printed hand-powered Centrifuge, as a novel alternative to standard benchtop Centrifuges. Based on the design principles of a paper-based Centrifuge, this 3D-printed instrument increases the volume capacity to 2 mL and can reach hand-powered centrifugation speeds up to 6,000 rpm. The 3D-Fuge devices presented here are capable of centrifugation of a wide variety of different solutions such as spinning down samples for biomarker applications and performing nucleotide extractions as part of a portable molecular lab setup. We introduce the design and proof-of-principle trials that demonstrate the utility of low-cost 3D printed Centrifuges for use in remote and educational settings.

Gaurav Byagathvalli - One of the best experts on this subject based on the ideXlab platform.

  • a 3d printed hand powered Centrifuge for molecular biology
    PLOS Biology, 2019
    Co-Authors: Gaurav Byagathvalli, Aaron Pomerantz, Soham Sinha, Janet Standeven, Saad M Bhamla
    Abstract:

    The Centrifuge is an essential tool for many aspects of research and medical diagnostics. However, conventional Centrifuges are often inaccessible outside of standard laboratory settings, such as remote field sites, because they require a constant external power source and can be prohibitively costly in resource-limited settings and Science, technology, engineering, and mathematics (STEM)-focused programs. Here we present the 3D-Fuge, a 3D-printed hand-powered Centrifuge, as a novel alternative to standard benchtop Centrifuges. Based on the design principles of a paper-based Centrifuge, this 3D-printed instrument increases the volume capacity to 2 mL and can reach hand-powered centrifugation speeds up to 6,000 rpm. The 3D-Fuge devices presented here are capable of centrifugation of a wide variety of different solutions such as spinning down samples for biomarker applications and performing nucleotide extractions as part of a portable molecular lab setup. We introduce the design and proof-of-principle trials that demonstrate the utility of low-cost 3D-printed Centrifuges for use in remote field biology and educational settings.

  • a 3d printed hand powered Centrifuge for molecular biology
    bioRxiv, 2019
    Co-Authors: Gaurav Byagathvalli, Aaron Pomerantz, Soham Sinha, Janet Standeven, Saad M Bhamla
    Abstract:

    The Centrifuge is an essential tool for many aspects of research and medical diagnostics. However, conventional Centrifuges are often inaccessible outside of conventional laboratory settings, such as remote field sites, require a constant external power source, and can be prohibitively costly in resource-limited settings and STEM-focused programs. Here we present the 3D-Fuge, a 3D-printed hand-powered Centrifuge, as a novel alternative to standard benchtop Centrifuges. Based on the design principles of a paper-based Centrifuge, this 3D-printed instrument increases the volume capacity to 2 mL and can reach hand-powered centrifugation speeds up to 6,000 rpm. The 3D-Fuge devices presented here are capable of centrifugation of a wide variety of different solutions such as spinning down samples for biomarker applications and performing nucleotide extractions as part of a portable molecular lab setup. We introduce the design and proof-of-principle trials that demonstrate the utility of low-cost 3D printed Centrifuges for use in remote and educational settings.

Tjatoer Welasih - One of the best experts on this subject based on the ideXlab platform.

  • PEMBUATAN VIRGIN COCONUT OIL (VCO) DENGAN METODE SENTRIFUGASI
    2013
    Co-Authors: Nur Hapsari, Tjatoer Welasih
    Abstract:

    Virgin Coconut Oil (VCO) is the oil produced from fresh coconuts. Unlike the ordinary coconut  oil,  Virgin  Coconut  Oil  (VCO)  is  produced  not  by the  addition  of  chemicals  or processes  that  use  high  heat.  Virgin  Coconut  Oil  (VCO)  beneficial  to  health,  this  is  because Virgin Coconut Oil (VCO) contains many medium chain fatty acids (Medium Chain Fatty Acid /  MCFA).  MCFA  are  most  in  the  Virgin  Coconut Oil  (VCO)  is  Lauric  acid  (Lauric  Acid). Properties  that  is  easily  absorbed  MCFA  will  increase  metabolism.  The  addition  of  energy produced  by  the  metabolism  of  this  produces  stimulating  effects  in  the  human  body  thereby increasing the level of energy produced.  Core  processes  in  the  manufacture  of  Virgin  Coconut  Oil  (VCO)  is  located  on  the separation of oil from water and protein. Many obstacles must be faced for separating oil from water  and  proteins,  such  as  in  the  fermentation  process,  the  separation  process takes  a  long time to get the Virgin Coconut Oil (VCO). While the centrifugation, the separation is done by utilizing the weight of the lighter oil. Although I do not require centrifugation long time but the way it has in the purity of the product constraints.  This research was conducted by using coconut cream from coconut milk is silenced for 3  hours.  Then  the  cream  is  incorporated  into  the  instrument  with  speed  Centrifuges  spin 600,700,800,900 and 1000 rpm with vaiasion  times very; 30, 45, 60.75 and 90 minutes after that  coconut  cream  layer  divided  into  3  layers  of  oil  that  is,  the  middle  layer  of  protein (blondo)  and  next  underwater  layer  silenced  for  8,10,12,14,  and  16  hours  .  The  results  of virgin  coconut  oil  (VCO)  is  obtained  and  then  analyzed  with  the  parameters  water  content, protein content, Lauric acid, color and odor.  From the research, obtained the best conditions of rotational speed Centrifuges at 1000 rpm and Centrifuges turnaround time 90 minutes and 8 hours stagnand time with the quality of Virgin  Coconut  Oil  (VCO)  which  produced  yield:  Lauric  acid  yield  of  36.67%  and  52.23% results. Keywords: sentrifuge, coconut cream,,% yield results, VCO

Nurhapsari - One of the best experts on this subject based on the ideXlab platform.

  • PEMBUATAN VIRGIN COCONUT OIL (VCO) DENGAN MENGGUNAKANMETODE SENTRIFUGASI
    2009
    Co-Authors: Wilasih Catur, Nurhapsari
    Abstract:

    Virgin Coconut Oil (VCO) is the oil produced from fresh coconuts. Unlike the ordinarycoconut oil, Virgin Coconut Oil (VCO) is produced not by the addition of chemicals orprocesses that use high heat. Virgin Coconut Oil (VCO) beneficial to health, this is because Virgin Coconut Oil (VCO) contains many medium chain fatty acids (Medium Chain Fatty Acid / MCFA). MCFA are most in the Virgin Coconut Oil (VCO) is Lauric acid (Lauric Acid). Properties that is easily absorbed MCFA will increase metabolism. The addition of energy produced by the metabolism of this produces stimulating effects in the human body thereby increasing the level of energy produced. Core processes in the manufacture of Virgin Coconut Oil (VCO) is located on the separation of oil from water and protein. Many obstacles must be faced for separating oil from water and proteins, such as in the fermentation process, the separation process takes a long time to get the Virgin Coconut Oil (VCO). While the centrifugation, the separation is done by utilizing the weight of the lighter oil. Although I do not require centrifugation long time but the way it has in the purity of the product constraints. This research was conducted by using coconut cream from coconut milk is silenced for 3 hours. Then the cream is incorporated into the instrument with speed Centrifuges spin 600,700,800,900 and 1000 rpm with Centrifuges turnaround times vary; 30, 45, 60.75 and 90 minutes after that coconut cream layer divided into 3 layers of oil that is, themiddle layer of protein (blondo) and next underwater layer silenced for 8,10,12,14, and 16 hours . The results of virgin coconut oil (VCO) is obtained and then analyzed with the parameters water content, protein content, Lauric acid, color and odor. From the research, obtained the best conditions of rotational speed Centrifuges at 1000 rpm and Centrifuges turnaround time 90 minutes and 8 hours stagnand time with the quality of Virgin Coconut Oil (VCO) which produced yield: Lauric acid yield of 36.67% and 52.23% results. Keywords: sentrifuge, coconut cream,,% yield results, VCO

Aaron Pomerantz - One of the best experts on this subject based on the ideXlab platform.

  • a 3d printed hand powered Centrifuge for molecular biology
    PLOS Biology, 2019
    Co-Authors: Gaurav Byagathvalli, Aaron Pomerantz, Soham Sinha, Janet Standeven, Saad M Bhamla
    Abstract:

    The Centrifuge is an essential tool for many aspects of research and medical diagnostics. However, conventional Centrifuges are often inaccessible outside of standard laboratory settings, such as remote field sites, because they require a constant external power source and can be prohibitively costly in resource-limited settings and Science, technology, engineering, and mathematics (STEM)-focused programs. Here we present the 3D-Fuge, a 3D-printed hand-powered Centrifuge, as a novel alternative to standard benchtop Centrifuges. Based on the design principles of a paper-based Centrifuge, this 3D-printed instrument increases the volume capacity to 2 mL and can reach hand-powered centrifugation speeds up to 6,000 rpm. The 3D-Fuge devices presented here are capable of centrifugation of a wide variety of different solutions such as spinning down samples for biomarker applications and performing nucleotide extractions as part of a portable molecular lab setup. We introduce the design and proof-of-principle trials that demonstrate the utility of low-cost 3D-printed Centrifuges for use in remote field biology and educational settings.

  • a 3d printed hand powered Centrifuge for molecular biology
    bioRxiv, 2019
    Co-Authors: Gaurav Byagathvalli, Aaron Pomerantz, Soham Sinha, Janet Standeven, Saad M Bhamla
    Abstract:

    The Centrifuge is an essential tool for many aspects of research and medical diagnostics. However, conventional Centrifuges are often inaccessible outside of conventional laboratory settings, such as remote field sites, require a constant external power source, and can be prohibitively costly in resource-limited settings and STEM-focused programs. Here we present the 3D-Fuge, a 3D-printed hand-powered Centrifuge, as a novel alternative to standard benchtop Centrifuges. Based on the design principles of a paper-based Centrifuge, this 3D-printed instrument increases the volume capacity to 2 mL and can reach hand-powered centrifugation speeds up to 6,000 rpm. The 3D-Fuge devices presented here are capable of centrifugation of a wide variety of different solutions such as spinning down samples for biomarker applications and performing nucleotide extractions as part of a portable molecular lab setup. We introduce the design and proof-of-principle trials that demonstrate the utility of low-cost 3D printed Centrifuges for use in remote and educational settings.