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Luiz Carlos Marques Vanderlei - One of the best experts on this subject based on the ideXlab platform.

  • effects of the administration of a catalase inhibitor into the fourth Cerebral Ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke
    Clinics, 2013
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Fernando Luiz Affonso Fonseca, Luciano Miller Reis Rodrigues, Fernando Adami, Luiz Carlos Marques Vanderlei, Lucas Lima Ferreira, Celso Ferreira
    Abstract:

    OBJECTIVE: Previous studies have demonstrated a relationship between brain oxidative stress and cardiovascular regulation. We evaluated the effects of central catalase inhibition on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke. METHODS: Male Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SH) (16 weeks old) were implanted with a stainless steel guide cannula leading into the fourth Cerebral Ventricle (4th V). The femoral artery and vein were cannulated for arterial pressure and heart rate measurement and drug infusion, respectively. The rats were exposed to sidestream cigarette smoke for 180 minutes/day, 5 days/week for 3 weeks (CO: 100-300 ppm). The baroreflex was tested using a pressor dose of phenylephrine (8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (50 μg/kg, bolus). Cardiovascular responses were evaluated before and 5, 15, 30 and 60 minutes after injection of a catalase inhibitor (3-amino-1,2,4-triazole, 0.001 g/100 μL) into the 4th V. RESULTS: Vehicle administration into the 4th V did not affect the cardiovascular response, whereas administration of the central catalase inhibitor increased the basal HR and attenuated the bradycardic peak (p<0.05) to a greater extent in WKY rats exposed to sidestream cigarette smoke than in WKY rats exposed to fresh air. However, in spontaneously hypertensive rats, the effect of the catalase inhibitor treatment was stronger in the fresh air condition (p<0.05). CONCLUSION: Administration of a catalase inhibitor into the 4th V combined with exposure to sidestream cigarette smoke has a stronger effect in WKY rats than in SH rats.

  • sidestream cigarette smoke effects on cardiovascular responses in conscious rats involvement of oxidative stress in the fourth Cerebral Ventricle
    BMC Cardiovascular Disorders, 2012
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Celso Ferreira, Fernando Luiz Affonso Fonseca, Carlos Bandeira De Mello Monteiro, Fernando Adami, Valdelias Xavier, Moacir Fernandes De Godoy, Luiz Carlos Marques Vanderlei
    Abstract:

    Background: Cigarette exposure increases brain oxidative stress. The literature showed that increased brain oxidative stress affects cardiovascular regulation. However, no previous study investigated the involvement of brain oxidative stress in animals exposed to cigarette and its relationship with cardiovascular regulation. We aimed to evaluate the effects of central catalase inhibition on baroreflex and cardiovascular responses in rats exposed to sidestream cigarette smoke (SSCS). Methods: We evaluated males Wistar rats (320-370 g), which were implanted with a stainless steel guide cannula into the fourth Cerebral Ventricle (4th V). Femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. Rats were exposed to SSCS during three weeks, 180 minutes, 5 days/week (CO: 100-300 ppm). Baroreflex was tested with a pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) to induce bradycardic reflex and a depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus) to induce tachycardic reflex. Cardiovascular responses were evaluated before, 5, 15, 30 and 60 minutes after 3-amino1,2,4-triazole (ATZ, catalase inhibitor, 0.001 g/100 μL) injection into the 4th V. Results: Central catalase inhibition increased basal HR in the control group during the first 5 minutes. SSCS exposure increased basal HR and attenuated bradycardic peak during the first 15 minutes. Conclusion: We suggest that SSCS exposure affects cardiovascular regulation through its influence on catalase activity.

Vitor Engrácia Valenti - One of the best experts on this subject based on the ideXlab platform.

  • cardiovascular responses induced by catalase inhibitior into the fourth Cerebral Ventricle is changed in wistar rats exposed to sidestream cigarette smoke
    International journal of health sciences, 2013
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Fernando Adami, Fernando La Fonseca, Joseluiz Figueiredo, Celso Ferreira
    Abstract:

    Objectives: This experimental study aimed to evaluate the effects of central catalase inhibition on cardiovascular responses in rats exposed to sidestream cigarette smoke (SSCS) for 3 weeks. Methodology: A total of 20 males Wistar rats (320-370g) were implanted with a stainless steel guide cannula into the fourth Cerebral Ventricle (4 th V). Femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. Rats were exposed to SSCS for three weeks, 180 minutes per day, 5 days/week [carbon monoxide (CO): 100-300 ppm)]. Baroreflex was tested with one pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) and one depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus). Cardiovascular responses were evaluated before and 15 minutes after 3-amino-1, 2, 4-triazole (ATZ, catalase inhibitor, 0.001g/100μL) injection into the 4 th V. Results: Vehicle treatment into the 4 th V did not change cardiovascular responses. Central catalase inhibition increased tachycardic peak, attenuated bradycardic peak and reduced HR range at 15 minutes, increased MAP at 5, 15 and 30 min and increased HR at 5 and 15 min. In rats exposed to SSCS, central ATZ increased basal MAP after 5 min and increased HR at 5, 15 and 30 minutes, respectively, and attenuated bradycardic peak at 15 minutes. Conclusion: This study suggests that brain oxidative stress caused by SSCS influences autonomic regulation of the cardiovascular system.

  • effects of the administration of a catalase inhibitor into the fourth Cerebral Ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke
    Clinics, 2013
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Fernando Luiz Affonso Fonseca, Luciano Miller Reis Rodrigues, Fernando Adami, Luiz Carlos Marques Vanderlei, Lucas Lima Ferreira, Celso Ferreira
    Abstract:

    OBJECTIVE: Previous studies have demonstrated a relationship between brain oxidative stress and cardiovascular regulation. We evaluated the effects of central catalase inhibition on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke. METHODS: Male Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SH) (16 weeks old) were implanted with a stainless steel guide cannula leading into the fourth Cerebral Ventricle (4th V). The femoral artery and vein were cannulated for arterial pressure and heart rate measurement and drug infusion, respectively. The rats were exposed to sidestream cigarette smoke for 180 minutes/day, 5 days/week for 3 weeks (CO: 100-300 ppm). The baroreflex was tested using a pressor dose of phenylephrine (8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (50 μg/kg, bolus). Cardiovascular responses were evaluated before and 5, 15, 30 and 60 minutes after injection of a catalase inhibitor (3-amino-1,2,4-triazole, 0.001 g/100 μL) into the 4th V. RESULTS: Vehicle administration into the 4th V did not affect the cardiovascular response, whereas administration of the central catalase inhibitor increased the basal HR and attenuated the bradycardic peak (p<0.05) to a greater extent in WKY rats exposed to sidestream cigarette smoke than in WKY rats exposed to fresh air. However, in spontaneously hypertensive rats, the effect of the catalase inhibitor treatment was stronger in the fresh air condition (p<0.05). CONCLUSION: Administration of a catalase inhibitor into the 4th V combined with exposure to sidestream cigarette smoke has a stronger effect in WKY rats than in SH rats.

  • sidestream cigarette smoke effects on cardiovascular responses in conscious rats involvement of oxidative stress in the fourth Cerebral Ventricle
    BMC Cardiovascular Disorders, 2012
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Celso Ferreira, Fernando Luiz Affonso Fonseca, Carlos Bandeira De Mello Monteiro, Fernando Adami, Valdelias Xavier, Moacir Fernandes De Godoy, Luiz Carlos Marques Vanderlei
    Abstract:

    Background: Cigarette exposure increases brain oxidative stress. The literature showed that increased brain oxidative stress affects cardiovascular regulation. However, no previous study investigated the involvement of brain oxidative stress in animals exposed to cigarette and its relationship with cardiovascular regulation. We aimed to evaluate the effects of central catalase inhibition on baroreflex and cardiovascular responses in rats exposed to sidestream cigarette smoke (SSCS). Methods: We evaluated males Wistar rats (320-370 g), which were implanted with a stainless steel guide cannula into the fourth Cerebral Ventricle (4th V). Femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. Rats were exposed to SSCS during three weeks, 180 minutes, 5 days/week (CO: 100-300 ppm). Baroreflex was tested with a pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) to induce bradycardic reflex and a depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus) to induce tachycardic reflex. Cardiovascular responses were evaluated before, 5, 15, 30 and 60 minutes after 3-amino1,2,4-triazole (ATZ, catalase inhibitor, 0.001 g/100 μL) injection into the 4th V. Results: Central catalase inhibition increased basal HR in the control group during the first 5 minutes. SSCS exposure increased basal HR and attenuated bradycardic peak during the first 15 minutes. Conclusion: We suggest that SSCS exposure affects cardiovascular regulation through its influence on catalase activity.

  • the effects of catalase inhibition into the fourth Cerebral Ventricle on the bezold jarisch reflex in spontaneously hypertensive rats
    Journal of Integrative Neuroscience, 2011
    Co-Authors: Jose R Cisternas, Vitor Engrácia Valenti, Monica Akemi Sato, Fernando Luiz Affonso Fonseca, Paulo Hilario Nascimento Saldiva, Carlos Bandeira De Mello Monteiro, Modesto Leite Rolim Neto, Luciano Miller Reis Rodrigues, Luiz Carlos De Abreu
    Abstract:

    Many studies have investigated the role of oxidative stress on cardiovascular system in the brainstem of spontaneously hypertensive rats (SHR). However, we do not know yet if catalase inhibition influences cardiopulmonary reflex (Bezol-Jarisch reflex). Thus, we aimed to evaluate the effects of central catalase inhibition on cardiopulmonary reflex in SHR. Males Wistar Kyoto (WKY) rats and SHR were implanted with a stainless steel guide cannula into the fourth Cerebral Ventricle (4th V). The femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. The cardiopulmonary reflex was tested with phenylbiguanide (PBG, 8 μg/kg, bolus, i.v.). Cardiopulmonary reflex was evaluated before and 15 minutes after 3-amino-1,2,4-triazole (ATZ, 0.01 g/100 μL) injection into the 4th V. Vehicle treatment did not change basal MAP and HR and cardiopulmonary reflex responses in SHR and WKY rats. Central ATZ increased hypotensive (p = 0.038) responses without influencing the bradycardic reflex (p = 0.287) in WKY rats. In SHR, ATZ increased hypotension (p = 0.0004) and bradycardic (p = 0.04) responses to i.v. PBG. No changes were observed regarding basal MAP and HR after ATZ injection in SHR and WKY rats. We suggest central catalase inhibition affects cardiopulmonary reflex with more intensity in SHR compared to WKY rats.

  • catalase inhibition into the fourth Cerebral Ventricle affects bradycardic parasympathetic response to increase in arterial pressure without changing the baroreflex
    Journal of Integrative Neuroscience, 2011
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Fernando Luiz Affonso Fonseca, Andres Ricardo Perez Riera, Celso Ferreira
    Abstract:

    Exogenous catalase influences neural control of cardiovascular system; however, we do not know yet if its inhibition into the fourth Cerebral Ventricle (4th V) influences baroreflex regulation. We evaluated the effects of central catalase inhibition on baroreflex in conscious Wistar rats. We used males Wistar rats (320–370 g), which were implanted with a stainless steel guide cannula into 4th V. The femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. After basal MAP and HR recordings, the baroreflex was tested with a pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus). Baroreflex was evaluated before 5, 15, 30 and 60 minutes after 3-amino-1, 2, 4-triazole (ATZ, 0.001 g/100 μL) injection into the 4th V. Vehicle treatment did not change baroreflex responses. ATZ attenuated bradycardic peak and reduced HR range at 30 minutes. ATZ into the 4th V reduced bradycardic and tachycardic reflex responses to increase and decrease MAP, respectively (p < 0.05) 30 minutes after its microinjection without significantly changing the basal MAP and HR. In conclusion, central catalase inhibition influenced the highest parasympathetic response to MAP increase in conscious Wistar rats without change baroreflex gain.

Celso Ferreira - One of the best experts on this subject based on the ideXlab platform.

  • cardiovascular responses induced by catalase inhibitior into the fourth Cerebral Ventricle is changed in wistar rats exposed to sidestream cigarette smoke
    International journal of health sciences, 2013
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Fernando Adami, Fernando La Fonseca, Joseluiz Figueiredo, Celso Ferreira
    Abstract:

    Objectives: This experimental study aimed to evaluate the effects of central catalase inhibition on cardiovascular responses in rats exposed to sidestream cigarette smoke (SSCS) for 3 weeks. Methodology: A total of 20 males Wistar rats (320-370g) were implanted with a stainless steel guide cannula into the fourth Cerebral Ventricle (4 th V). Femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. Rats were exposed to SSCS for three weeks, 180 minutes per day, 5 days/week [carbon monoxide (CO): 100-300 ppm)]. Baroreflex was tested with one pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) and one depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus). Cardiovascular responses were evaluated before and 15 minutes after 3-amino-1, 2, 4-triazole (ATZ, catalase inhibitor, 0.001g/100μL) injection into the 4 th V. Results: Vehicle treatment into the 4 th V did not change cardiovascular responses. Central catalase inhibition increased tachycardic peak, attenuated bradycardic peak and reduced HR range at 15 minutes, increased MAP at 5, 15 and 30 min and increased HR at 5 and 15 min. In rats exposed to SSCS, central ATZ increased basal MAP after 5 min and increased HR at 5, 15 and 30 minutes, respectively, and attenuated bradycardic peak at 15 minutes. Conclusion: This study suggests that brain oxidative stress caused by SSCS influences autonomic regulation of the cardiovascular system.

  • effects of the administration of a catalase inhibitor into the fourth Cerebral Ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke
    Clinics, 2013
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Fernando Luiz Affonso Fonseca, Luciano Miller Reis Rodrigues, Fernando Adami, Luiz Carlos Marques Vanderlei, Lucas Lima Ferreira, Celso Ferreira
    Abstract:

    OBJECTIVE: Previous studies have demonstrated a relationship between brain oxidative stress and cardiovascular regulation. We evaluated the effects of central catalase inhibition on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke. METHODS: Male Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SH) (16 weeks old) were implanted with a stainless steel guide cannula leading into the fourth Cerebral Ventricle (4th V). The femoral artery and vein were cannulated for arterial pressure and heart rate measurement and drug infusion, respectively. The rats were exposed to sidestream cigarette smoke for 180 minutes/day, 5 days/week for 3 weeks (CO: 100-300 ppm). The baroreflex was tested using a pressor dose of phenylephrine (8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (50 μg/kg, bolus). Cardiovascular responses were evaluated before and 5, 15, 30 and 60 minutes after injection of a catalase inhibitor (3-amino-1,2,4-triazole, 0.001 g/100 μL) into the 4th V. RESULTS: Vehicle administration into the 4th V did not affect the cardiovascular response, whereas administration of the central catalase inhibitor increased the basal HR and attenuated the bradycardic peak (p<0.05) to a greater extent in WKY rats exposed to sidestream cigarette smoke than in WKY rats exposed to fresh air. However, in spontaneously hypertensive rats, the effect of the catalase inhibitor treatment was stronger in the fresh air condition (p<0.05). CONCLUSION: Administration of a catalase inhibitor into the 4th V combined with exposure to sidestream cigarette smoke has a stronger effect in WKY rats than in SH rats.

  • sidestream cigarette smoke effects on cardiovascular responses in conscious rats involvement of oxidative stress in the fourth Cerebral Ventricle
    BMC Cardiovascular Disorders, 2012
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Celso Ferreira, Fernando Luiz Affonso Fonseca, Carlos Bandeira De Mello Monteiro, Fernando Adami, Valdelias Xavier, Moacir Fernandes De Godoy, Luiz Carlos Marques Vanderlei
    Abstract:

    Background: Cigarette exposure increases brain oxidative stress. The literature showed that increased brain oxidative stress affects cardiovascular regulation. However, no previous study investigated the involvement of brain oxidative stress in animals exposed to cigarette and its relationship with cardiovascular regulation. We aimed to evaluate the effects of central catalase inhibition on baroreflex and cardiovascular responses in rats exposed to sidestream cigarette smoke (SSCS). Methods: We evaluated males Wistar rats (320-370 g), which were implanted with a stainless steel guide cannula into the fourth Cerebral Ventricle (4th V). Femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. Rats were exposed to SSCS during three weeks, 180 minutes, 5 days/week (CO: 100-300 ppm). Baroreflex was tested with a pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) to induce bradycardic reflex and a depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus) to induce tachycardic reflex. Cardiovascular responses were evaluated before, 5, 15, 30 and 60 minutes after 3-amino1,2,4-triazole (ATZ, catalase inhibitor, 0.001 g/100 μL) injection into the 4th V. Results: Central catalase inhibition increased basal HR in the control group during the first 5 minutes. SSCS exposure increased basal HR and attenuated bradycardic peak during the first 15 minutes. Conclusion: We suggest that SSCS exposure affects cardiovascular regulation through its influence on catalase activity.

  • catalase inhibition into the fourth Cerebral Ventricle affects bradycardic parasympathetic response to increase in arterial pressure without changing the baroreflex
    Journal of Integrative Neuroscience, 2011
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Fernando Luiz Affonso Fonseca, Andres Ricardo Perez Riera, Celso Ferreira
    Abstract:

    Exogenous catalase influences neural control of cardiovascular system; however, we do not know yet if its inhibition into the fourth Cerebral Ventricle (4th V) influences baroreflex regulation. We evaluated the effects of central catalase inhibition on baroreflex in conscious Wistar rats. We used males Wistar rats (320–370 g), which were implanted with a stainless steel guide cannula into 4th V. The femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. After basal MAP and HR recordings, the baroreflex was tested with a pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus). Baroreflex was evaluated before 5, 15, 30 and 60 minutes after 3-amino-1, 2, 4-triazole (ATZ, 0.001 g/100 μL) injection into the 4th V. Vehicle treatment did not change baroreflex responses. ATZ attenuated bradycardic peak and reduced HR range at 30 minutes. ATZ into the 4th V reduced bradycardic and tachycardic reflex responses to increase and decrease MAP, respectively (p < 0.05) 30 minutes after its microinjection without significantly changing the basal MAP and HR. In conclusion, central catalase inhibition influenced the highest parasympathetic response to MAP increase in conscious Wistar rats without change baroreflex gain.

  • ATZ (3-amino-1,2,4-triazole) injected into the fourth Cerebral Ventricle influences the Bezold-Jarisch reflex in conscious rats
    Clinics (Sao Paulo Brazil), 2010
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Celso Ferreira
    Abstract:

    OBJECTIVES: Many studies have investigated the importance of oxidative stress on the cardiovascular system. In this study we evaluated the effects of central catalase inhibition on cardiopulmonary reflex in conscious Wistar rats. METHODS: Male Wistar rats were implanted with a stainless steel guide cannula in the fourth Cerebral Ventricle. The femoral artery and vein were cannulated for mean arterial pressure and heart rate measurement and for drug infusion, respectively. After basal mean arterial pressure and heart rate recordings, the cardiopulmonary reflex was tested with a dose of phenylbiguanide (PBG, 8 μg/kg, bolus). Cardiopulmonary reflex was evaluated before and μl15 minutes after 1.0 μl 3-amino-1,2,4-triazole (ATZ, 0.01g/100μl)0.01 g/100 μl) injection into the fourth Cerebral Ventricle. Vehicle treatment did not change cardiopulmonary reflex responses. RESULTS: Central ATZ significantly increased hypotensive responses without influencing the bradycardic reflex. CONCLUSION: ATZ injected into the fourth Cerebral Ventricle increases sympathetic inhibition but does not change the parasympathetic component of the cardiopulmonary reflex in conscious Wistar rats.

Luiz Carlos De Abreu - One of the best experts on this subject based on the ideXlab platform.

  • cardiovascular responses induced by catalase inhibitior into the fourth Cerebral Ventricle is changed in wistar rats exposed to sidestream cigarette smoke
    International journal of health sciences, 2013
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Fernando Adami, Fernando La Fonseca, Joseluiz Figueiredo, Celso Ferreira
    Abstract:

    Objectives: This experimental study aimed to evaluate the effects of central catalase inhibition on cardiovascular responses in rats exposed to sidestream cigarette smoke (SSCS) for 3 weeks. Methodology: A total of 20 males Wistar rats (320-370g) were implanted with a stainless steel guide cannula into the fourth Cerebral Ventricle (4 th V). Femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. Rats were exposed to SSCS for three weeks, 180 minutes per day, 5 days/week [carbon monoxide (CO): 100-300 ppm)]. Baroreflex was tested with one pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) and one depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus). Cardiovascular responses were evaluated before and 15 minutes after 3-amino-1, 2, 4-triazole (ATZ, catalase inhibitor, 0.001g/100μL) injection into the 4 th V. Results: Vehicle treatment into the 4 th V did not change cardiovascular responses. Central catalase inhibition increased tachycardic peak, attenuated bradycardic peak and reduced HR range at 15 minutes, increased MAP at 5, 15 and 30 min and increased HR at 5 and 15 min. In rats exposed to SSCS, central ATZ increased basal MAP after 5 min and increased HR at 5, 15 and 30 minutes, respectively, and attenuated bradycardic peak at 15 minutes. Conclusion: This study suggests that brain oxidative stress caused by SSCS influences autonomic regulation of the cardiovascular system.

  • effects of the administration of a catalase inhibitor into the fourth Cerebral Ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke
    Clinics, 2013
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Fernando Luiz Affonso Fonseca, Luciano Miller Reis Rodrigues, Fernando Adami, Luiz Carlos Marques Vanderlei, Lucas Lima Ferreira, Celso Ferreira
    Abstract:

    OBJECTIVE: Previous studies have demonstrated a relationship between brain oxidative stress and cardiovascular regulation. We evaluated the effects of central catalase inhibition on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke. METHODS: Male Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SH) (16 weeks old) were implanted with a stainless steel guide cannula leading into the fourth Cerebral Ventricle (4th V). The femoral artery and vein were cannulated for arterial pressure and heart rate measurement and drug infusion, respectively. The rats were exposed to sidestream cigarette smoke for 180 minutes/day, 5 days/week for 3 weeks (CO: 100-300 ppm). The baroreflex was tested using a pressor dose of phenylephrine (8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (50 μg/kg, bolus). Cardiovascular responses were evaluated before and 5, 15, 30 and 60 minutes after injection of a catalase inhibitor (3-amino-1,2,4-triazole, 0.001 g/100 μL) into the 4th V. RESULTS: Vehicle administration into the 4th V did not affect the cardiovascular response, whereas administration of the central catalase inhibitor increased the basal HR and attenuated the bradycardic peak (p<0.05) to a greater extent in WKY rats exposed to sidestream cigarette smoke than in WKY rats exposed to fresh air. However, in spontaneously hypertensive rats, the effect of the catalase inhibitor treatment was stronger in the fresh air condition (p<0.05). CONCLUSION: Administration of a catalase inhibitor into the 4th V combined with exposure to sidestream cigarette smoke has a stronger effect in WKY rats than in SH rats.

  • sidestream cigarette smoke effects on cardiovascular responses in conscious rats involvement of oxidative stress in the fourth Cerebral Ventricle
    BMC Cardiovascular Disorders, 2012
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Celso Ferreira, Fernando Luiz Affonso Fonseca, Carlos Bandeira De Mello Monteiro, Fernando Adami, Valdelias Xavier, Moacir Fernandes De Godoy, Luiz Carlos Marques Vanderlei
    Abstract:

    Background: Cigarette exposure increases brain oxidative stress. The literature showed that increased brain oxidative stress affects cardiovascular regulation. However, no previous study investigated the involvement of brain oxidative stress in animals exposed to cigarette and its relationship with cardiovascular regulation. We aimed to evaluate the effects of central catalase inhibition on baroreflex and cardiovascular responses in rats exposed to sidestream cigarette smoke (SSCS). Methods: We evaluated males Wistar rats (320-370 g), which were implanted with a stainless steel guide cannula into the fourth Cerebral Ventricle (4th V). Femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. Rats were exposed to SSCS during three weeks, 180 minutes, 5 days/week (CO: 100-300 ppm). Baroreflex was tested with a pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) to induce bradycardic reflex and a depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus) to induce tachycardic reflex. Cardiovascular responses were evaluated before, 5, 15, 30 and 60 minutes after 3-amino1,2,4-triazole (ATZ, catalase inhibitor, 0.001 g/100 μL) injection into the 4th V. Results: Central catalase inhibition increased basal HR in the control group during the first 5 minutes. SSCS exposure increased basal HR and attenuated bradycardic peak during the first 15 minutes. Conclusion: We suggest that SSCS exposure affects cardiovascular regulation through its influence on catalase activity.

  • the effects of catalase inhibition into the fourth Cerebral Ventricle on the bezold jarisch reflex in spontaneously hypertensive rats
    Journal of Integrative Neuroscience, 2011
    Co-Authors: Jose R Cisternas, Vitor Engrácia Valenti, Monica Akemi Sato, Fernando Luiz Affonso Fonseca, Paulo Hilario Nascimento Saldiva, Carlos Bandeira De Mello Monteiro, Modesto Leite Rolim Neto, Luciano Miller Reis Rodrigues, Luiz Carlos De Abreu
    Abstract:

    Many studies have investigated the role of oxidative stress on cardiovascular system in the brainstem of spontaneously hypertensive rats (SHR). However, we do not know yet if catalase inhibition influences cardiopulmonary reflex (Bezol-Jarisch reflex). Thus, we aimed to evaluate the effects of central catalase inhibition on cardiopulmonary reflex in SHR. Males Wistar Kyoto (WKY) rats and SHR were implanted with a stainless steel guide cannula into the fourth Cerebral Ventricle (4th V). The femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. The cardiopulmonary reflex was tested with phenylbiguanide (PBG, 8 μg/kg, bolus, i.v.). Cardiopulmonary reflex was evaluated before and 15 minutes after 3-amino-1,2,4-triazole (ATZ, 0.01 g/100 μL) injection into the 4th V. Vehicle treatment did not change basal MAP and HR and cardiopulmonary reflex responses in SHR and WKY rats. Central ATZ increased hypotensive (p = 0.038) responses without influencing the bradycardic reflex (p = 0.287) in WKY rats. In SHR, ATZ increased hypotension (p = 0.0004) and bradycardic (p = 0.04) responses to i.v. PBG. No changes were observed regarding basal MAP and HR after ATZ injection in SHR and WKY rats. We suggest central catalase inhibition affects cardiopulmonary reflex with more intensity in SHR compared to WKY rats.

  • catalase inhibition into the fourth Cerebral Ventricle affects bradycardic parasympathetic response to increase in arterial pressure without changing the baroreflex
    Journal of Integrative Neuroscience, 2011
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Fernando Luiz Affonso Fonseca, Andres Ricardo Perez Riera, Celso Ferreira
    Abstract:

    Exogenous catalase influences neural control of cardiovascular system; however, we do not know yet if its inhibition into the fourth Cerebral Ventricle (4th V) influences baroreflex regulation. We evaluated the effects of central catalase inhibition on baroreflex in conscious Wistar rats. We used males Wistar rats (320–370 g), which were implanted with a stainless steel guide cannula into 4th V. The femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. After basal MAP and HR recordings, the baroreflex was tested with a pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus). Baroreflex was evaluated before 5, 15, 30 and 60 minutes after 3-amino-1, 2, 4-triazole (ATZ, 0.001 g/100 μL) injection into the 4th V. Vehicle treatment did not change baroreflex responses. ATZ attenuated bradycardic peak and reduced HR range at 30 minutes. ATZ into the 4th V reduced bradycardic and tachycardic reflex responses to increase and decrease MAP, respectively (p < 0.05) 30 minutes after its microinjection without significantly changing the basal MAP and HR. In conclusion, central catalase inhibition influenced the highest parasympathetic response to MAP increase in conscious Wistar rats without change baroreflex gain.

Fernando Adami - One of the best experts on this subject based on the ideXlab platform.

  • cardiovascular responses induced by catalase inhibitior into the fourth Cerebral Ventricle is changed in wistar rats exposed to sidestream cigarette smoke
    International journal of health sciences, 2013
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Fernando Adami, Fernando La Fonseca, Joseluiz Figueiredo, Celso Ferreira
    Abstract:

    Objectives: This experimental study aimed to evaluate the effects of central catalase inhibition on cardiovascular responses in rats exposed to sidestream cigarette smoke (SSCS) for 3 weeks. Methodology: A total of 20 males Wistar rats (320-370g) were implanted with a stainless steel guide cannula into the fourth Cerebral Ventricle (4 th V). Femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. Rats were exposed to SSCS for three weeks, 180 minutes per day, 5 days/week [carbon monoxide (CO): 100-300 ppm)]. Baroreflex was tested with one pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) and one depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus). Cardiovascular responses were evaluated before and 15 minutes after 3-amino-1, 2, 4-triazole (ATZ, catalase inhibitor, 0.001g/100μL) injection into the 4 th V. Results: Vehicle treatment into the 4 th V did not change cardiovascular responses. Central catalase inhibition increased tachycardic peak, attenuated bradycardic peak and reduced HR range at 15 minutes, increased MAP at 5, 15 and 30 min and increased HR at 5 and 15 min. In rats exposed to SSCS, central ATZ increased basal MAP after 5 min and increased HR at 5, 15 and 30 minutes, respectively, and attenuated bradycardic peak at 15 minutes. Conclusion: This study suggests that brain oxidative stress caused by SSCS influences autonomic regulation of the cardiovascular system.

  • effects of the administration of a catalase inhibitor into the fourth Cerebral Ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke
    Clinics, 2013
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Fernando Luiz Affonso Fonseca, Luciano Miller Reis Rodrigues, Fernando Adami, Luiz Carlos Marques Vanderlei, Lucas Lima Ferreira, Celso Ferreira
    Abstract:

    OBJECTIVE: Previous studies have demonstrated a relationship between brain oxidative stress and cardiovascular regulation. We evaluated the effects of central catalase inhibition on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke. METHODS: Male Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SH) (16 weeks old) were implanted with a stainless steel guide cannula leading into the fourth Cerebral Ventricle (4th V). The femoral artery and vein were cannulated for arterial pressure and heart rate measurement and drug infusion, respectively. The rats were exposed to sidestream cigarette smoke for 180 minutes/day, 5 days/week for 3 weeks (CO: 100-300 ppm). The baroreflex was tested using a pressor dose of phenylephrine (8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (50 μg/kg, bolus). Cardiovascular responses were evaluated before and 5, 15, 30 and 60 minutes after injection of a catalase inhibitor (3-amino-1,2,4-triazole, 0.001 g/100 μL) into the 4th V. RESULTS: Vehicle administration into the 4th V did not affect the cardiovascular response, whereas administration of the central catalase inhibitor increased the basal HR and attenuated the bradycardic peak (p<0.05) to a greater extent in WKY rats exposed to sidestream cigarette smoke than in WKY rats exposed to fresh air. However, in spontaneously hypertensive rats, the effect of the catalase inhibitor treatment was stronger in the fresh air condition (p<0.05). CONCLUSION: Administration of a catalase inhibitor into the 4th V combined with exposure to sidestream cigarette smoke has a stronger effect in WKY rats than in SH rats.

  • sidestream cigarette smoke effects on cardiovascular responses in conscious rats involvement of oxidative stress in the fourth Cerebral Ventricle
    BMC Cardiovascular Disorders, 2012
    Co-Authors: Vitor Engrácia Valenti, Luiz Carlos De Abreu, Monica Akemi Sato, Celso Ferreira, Fernando Luiz Affonso Fonseca, Carlos Bandeira De Mello Monteiro, Fernando Adami, Valdelias Xavier, Moacir Fernandes De Godoy, Luiz Carlos Marques Vanderlei
    Abstract:

    Background: Cigarette exposure increases brain oxidative stress. The literature showed that increased brain oxidative stress affects cardiovascular regulation. However, no previous study investigated the involvement of brain oxidative stress in animals exposed to cigarette and its relationship with cardiovascular regulation. We aimed to evaluate the effects of central catalase inhibition on baroreflex and cardiovascular responses in rats exposed to sidestream cigarette smoke (SSCS). Methods: We evaluated males Wistar rats (320-370 g), which were implanted with a stainless steel guide cannula into the fourth Cerebral Ventricle (4th V). Femoral artery and vein were cannulated for mean arterial pressure (MAP) and heart rate (HR) measurement and drug infusion, respectively. Rats were exposed to SSCS during three weeks, 180 minutes, 5 days/week (CO: 100-300 ppm). Baroreflex was tested with a pressor dose of phenylephrine (PHE, 8 μg/kg, bolus) to induce bradycardic reflex and a depressor dose of sodium nitroprusside (SNP, 50 μg/kg, bolus) to induce tachycardic reflex. Cardiovascular responses were evaluated before, 5, 15, 30 and 60 minutes after 3-amino1,2,4-triazole (ATZ, catalase inhibitor, 0.001 g/100 μL) injection into the 4th V. Results: Central catalase inhibition increased basal HR in the control group during the first 5 minutes. SSCS exposure increased basal HR and attenuated bradycardic peak during the first 15 minutes. Conclusion: We suggest that SSCS exposure affects cardiovascular regulation through its influence on catalase activity.