The Experts below are selected from a list of 1419 Experts worldwide ranked by ideXlab platform
K A Sheikh - One of the best experts on this subject based on the ideXlab platform.
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the wound healing properties of channa striatus Cetrimide cream wound contraction and glycosaminoglycan measurement
Journal of Ethnopharmacology, 2000Co-Authors: Saringat Hj Baie, K A SheikhAbstract:Abstract Haruan has been proved to influence the different phases of wound healing process. The current research focuses on the effects of haruan on the different constituents of extracellular matrix of healing wounds in normal and diabetic rats. Anaesthetized normal and streptozotocin induced diabetic rats were provided with excision wounds at the back and then animals were divided into four groups as: group 1, wounds treated with Cetrimide+haruan cream; group 2, wounds treated with haruan cream; group 3, wounds treated with Cetrimide (commercial) cream; and group 4, wounds untreated and served as control. Animals were sacrificed after 3, 6, 9 and 12 days. These wounds were used to determine the hexosamine, protein, uronic acid and glycosaminoglycan contents and the wound contraction. The results suggested a marked increase (P
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the wound healing properties of channa striatus Cetrimide cream wound contraction and glycosaminoglycan measurement
Journal of Ethnopharmacology, 2000Co-Authors: Saringat Hj Baie, K A SheikhAbstract:Haruan has been proved to influence the different phases of wound healing process. The current research focuses on the effects of haruan on the different constituents of extracellular matrix of healing wounds in normal and diabetic rats. Anaesthetized normal and streptozotocin induced diabetic rats were provided with excision wounds at the back and then animals were divided into four groups as: group 1, wounds treated with Cetrimide+haruan cream; group 2, wounds treated with haruan cream; group 3, wounds treated with Cetrimide (commercial) cream; and group 4, wounds untreated and served as control. Animals were sacrificed after 3, 6, 9 and 12 days. These wounds were used to determine the hexosamine, protein, uronic acid and glycosaminoglycan contents and the wound contraction. The results suggested a marked increase (P<0.05) in the uronic acid, hexosamine and dermatan sulfate contents on day 3 of group 1 when compared with groups 2-4. Wound contraction of group 1 was also markedly enhanced of group 1 (P<0.01) when compared with groups 2- 4. On the basis of these results, we conclude that haruan enhances the synthesis of different glycosaminoglycans in healing wounds, which are the first component of extracellular matrix to be synthesized during the wound healing process. The enhanced levels of glycosaminoglycans may help in the formation of a resistant scar and enhanced wound contraction represents the positive influence of haruan on the fibroplastic phase of wound healing.
Saringat Hj Baie - One of the best experts on this subject based on the ideXlab platform.
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the wound healing properties of channa striatus Cetrimide cream wound contraction and glycosaminoglycan measurement
Journal of Ethnopharmacology, 2000Co-Authors: Saringat Hj Baie, K A SheikhAbstract:Abstract Haruan has been proved to influence the different phases of wound healing process. The current research focuses on the effects of haruan on the different constituents of extracellular matrix of healing wounds in normal and diabetic rats. Anaesthetized normal and streptozotocin induced diabetic rats were provided with excision wounds at the back and then animals were divided into four groups as: group 1, wounds treated with Cetrimide+haruan cream; group 2, wounds treated with haruan cream; group 3, wounds treated with Cetrimide (commercial) cream; and group 4, wounds untreated and served as control. Animals were sacrificed after 3, 6, 9 and 12 days. These wounds were used to determine the hexosamine, protein, uronic acid and glycosaminoglycan contents and the wound contraction. The results suggested a marked increase (P
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the wound healing properties of channa striatus Cetrimide cream wound contraction and glycosaminoglycan measurement
Journal of Ethnopharmacology, 2000Co-Authors: Saringat Hj Baie, K A SheikhAbstract:Haruan has been proved to influence the different phases of wound healing process. The current research focuses on the effects of haruan on the different constituents of extracellular matrix of healing wounds in normal and diabetic rats. Anaesthetized normal and streptozotocin induced diabetic rats were provided with excision wounds at the back and then animals were divided into four groups as: group 1, wounds treated with Cetrimide+haruan cream; group 2, wounds treated with haruan cream; group 3, wounds treated with Cetrimide (commercial) cream; and group 4, wounds untreated and served as control. Animals were sacrificed after 3, 6, 9 and 12 days. These wounds were used to determine the hexosamine, protein, uronic acid and glycosaminoglycan contents and the wound contraction. The results suggested a marked increase (P<0.05) in the uronic acid, hexosamine and dermatan sulfate contents on day 3 of group 1 when compared with groups 2-4. Wound contraction of group 1 was also markedly enhanced of group 1 (P<0.01) when compared with groups 2- 4. On the basis of these results, we conclude that haruan enhances the synthesis of different glycosaminoglycans in healing wounds, which are the first component of extracellular matrix to be synthesized during the wound healing process. The enhanced levels of glycosaminoglycans may help in the formation of a resistant scar and enhanced wound contraction represents the positive influence of haruan on the fibroplastic phase of wound healing.
Pilar Baca - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial activity of alexidine, chlorhexidine and Cetrimide against Streptococcus mutans biofilm
Annals of Clinical Microbiology and Antimicrobials, 2014Co-Authors: Matilde Ruiz-linares, Carmen Maria Ferrer-luque, Teresa Arias-moliz, Paula De Castro, Beatriz Aguado, Pilar BacaAbstract:Background The use of antimicrobial solutions has been recommended to disinfect demineralized dentin prior to placing the filling material. The aim of this study was to evaluate the ability of several antimicrobials in controlling Streptococcus mutans (SM) biofilm formed in dentin. Methods Antimicrobial activity of 0.2% and 2% chlorhexidine (CHX), 0.2% Cetrimide (CTR) and 0.2%, 0.5%, 1% and 2% alexidine (ALX) was assayed on 1-week SM biofilm formed on standardized coronal dentin blocks. Results of SM biofilm antimicrobial activity by different protocols were expressed as the kill percentage of biofilm and the term “eradication” was used to denote the kill of 100% of the bacterial population. To compare the efficacies of the different protocols the Student t test was used, previously subjecting data to the Anscombe transformation. Results All ALX concentrations tested and 0.2% CTR achieved a kill percentage higher than 99%, followed by 2% CHX with percentages above 96% (no statistically significant difference among them). Whereas 2% ALX and 0.2% CTR respectively showed eradication in 10 and 9 of the twelve specimens, 0.2% CHX did not produce eradication in any case. Conclusions The present study shows that, when used for one minute, 2% and 1% alexidine, and 0.2% Cetrimide, achieve eradication of Streptococcus mutans biofilm in most specimens when applied to a dentin-volumetric model.
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antimicrobial substantivity over time of chlorhexidine and Cetrimide
Journal of Endodontics, 2012Co-Authors: Pilar Baca, Maria Teresa Ariasmoliz, Pilar Junco, Francisca Castillo, Alberto Rodriguezarchilla, Carmen Maria FerrerluqueAbstract:Abstract Introduction To reach an adequate control of dentin infection and to promote success in endodontic therapy, antimicrobial irrigating solutions with confirmed substantivity are recommended. The aim of the present study was to evaluate the antimicrobial substantivity against Enterococcus faecalis of a dentin-volumetric unit exposed for 1 minute to chlorhexidine (CHX) and Cetrimide (CTR). Methods Standardized coronal dentin blocks of human molars, with and without collagen, were treated for 1 minute with 0.2% and 2% CHX and 0.2% CTR. Afterwards, they were exposed to E. faecalis suspension to determine the antimicrobial substantivity over a period of 60 days. Results were analyzed by means of Kaplan-Meier survival analysis ( P Results A direct relationship was seen between CHX concentration and survival time, and the most statistically significant results were obtained in specimens with collagen. CTR showed intermediate survival values close to those of 2% CHX. Conclusions The present study shows that 2% CHX used for 1 minute provides the longest substantivity followed by 0.2% CTR when applied to a dentin-volumetric model.
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antimicrobial activity of maleic acid and combinations of Cetrimide with chelating agents against enterococcus faecalis biofilm
Journal of Endodontics, 2010Co-Authors: Carmen Maria Ferrerluque, Maria Teresa Ariasmoliz, Maria Paloma Gonzalezrodriguez, Pilar BacaAbstract:Introduction: Chelating agents, such as EDTA or citric acid, can beincorporatedintoirrigants inorder to provide different properties in a single compound. Maleic acid has recently been proposed as an alternative irrigant to EDTA given its better smear layer removal and biocompatibility; however, its antimicrobial activity is not known. The purpose of the present study was therefore to evaluate the in vitro capacity of maleic acid in eradicating Enterococcus faecalis biofilms and to evaluate the combinations of Cetrimide with maleic acid, citric acid, and EDTA. Methods: E. faecalis biofilms were grown in the MBEC high-throughput device (Innovotech; Edmonton, Alberta, Canada) for 24 hours and exposed to the irrigating solutions for 30 seconds and 1 and2minutes.‘‘Eradication’’wasdefinedas100%bacterial kill. The Student t test was used to compare the efficacy of the irrigant combinations. Results: Maleic acid eradicated E. faecalis biofilms at a concentration of 0.88% after 30 seconds and at 0.11% after 2 minutes of contact time. When combined with 0.2% Cetrimide, it eradicated the biofilms at all three times of exposure. The combination of 0.2% Cetrimide with either 15% EDTA or 15% citric acid gave 100% bacterial kill after one minute of contact with the biofilms. Conclusions: Maleic acidshowedantimicrobial activity againstE. faecalis biofilm both alone or in association with Cetrimide from 30 seconds onward and the combination of EDTA and citric acid with Cetrimide eradicated biofilm after 1 minute of contact. (J Endod 2010;36:1673‐1675)
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eradication of enterococcus faecalis biofilms by Cetrimide and chlorhexidine
Journal of Endodontics, 2010Co-Authors: Maria Teresa Ariasmoliz, Carmen Maria Ferrerluque, Maria Paloma Gonzalezrodriguez, Mariano J Valderrama, Pilar BacaAbstract:Abstract Introduction Enterococcus faecalis is the most commonly isolated bacteria from root canals of teeth with persistent periapical periodontitis. Its ability to grow as a biofilm impedes the elimination of E. faecalis by using irrigating solutions. The purpose of this study was to assess the efficacy of Cetrimide and chlorhexidine (CHX), alone and in association, in combined and alternating form, in eradicating biofilms of E. faecalis. Methods Biofilms grown in the MBEC–high-throughput device for 24 hours were exposed to irrigating solutions for 30 seconds and 1 and 2 minutes. Eradication was defined as 100% kill of biofilm bacteria. The Student t test was used to compare the efficacy of the associations of the 2 irrigants. Results Cetrimide eradicated E. faecalis biofilms at concentrations of 0.5%, 0.0312%, and 0.0078% at 30 seconds and 1 and 2 minutes of contact time, respectively. CHX did not eradicate the biofilms at any of the concentrations (4% initial concentration) or times assayed. The association of 0.1% and 0.05% Cetrimide with any concentration of CHX, whether in combined or alternating application, effectively eradicated E. faecalis biofilms at all the contact times tested. Eradication was also achieved with 0.02% and 0.01% Cetrimide at 2 minutes. Statistical analysis revealed significantly better results with alternating rather than combined use of Cetrimide and CHX (P Conclusions The associated use of Cetrimide and CHX provided better results than their applications as single agents against E. faecalis biofilms, and the alternating application was significantly more effective than the combined mode of application.
Edson Zangiacomi Martinez - One of the best experts on this subject based on the ideXlab platform.
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eficacia de meios agar m pa c e agar Cetrimide no isolamento de pseudomonas aeruginosa em amostras de agua mineral Cetrimide and m pa c agar media efficiency for isolating pseudomonas aeruginosa from mineral water samples
2007Co-Authors: Beatriz Pisani, Marise Simoes, Maria Angela, Garnica Prandi, Maria Helena Martini, Teixeira Chiarini, Edson Zangiacomi MartinezAbstract:This study evaluated the efficacy of two selective media, M-PA-C agar and Cetrimide agar, for isolating P. aeruginosa cells at different incubation temperatures. P. aeruginosa recovery rates, as well as the selectivity of both media were compared. A random effects Bayesian model for binary data was used for performing statistical analysis, and a Poisson Bayesian model was applied to compare the potential of both media selectivity and P. aeruginosa recovery. A discernible difference on the media selectivity was evidenced, and M-PA-C medium presented a higher mean counting rate. P. aeruginosa recovery was similar in both media, and no significant difference was noted in three counts on M-PA-C at 42°C (208 CFU) , and on Cetrimide Agar at 35°C (231 CFU), at 10 -6 dilution. With regards to analyzed mineral water samples, no evidence of distinct occurrence rates of P. aeruginosa on either media, at
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eficacia de meios agar m pa c e agar Cetrimide no isolamento de pseudomonas aeruginosa em amostras de agua mineral
Revista do Instituto Adolfo Lutz (Impresso), 2007Co-Authors: Beatriz Pisani, Marise Simoes, Maria Helena Martini, Maria Angela Garnica Prandi, Paulo Flavio Teixeira Chiarini, Edson Zangiacomi MartinezAbstract:This study evaluated the efficacy of two selective media, M-PA-C agar and Cetrimide agar, for isolating P. aeruginosa cells at different incubation temperatures. P. aeruginosa recovery rates, as well as the selectivity of both media were compared. A random effects Bayesian model for binary data was used for performing statistical analysis, and a Poisson Bayesian model was applied to compare the potential of both media selectivity and P. aeruginosa recovery. A discernible difference on the media selectivity was evidenced, and M-PA-C medium presented a higher mean counting rate. P. aeruginosa recovery was similar in both media, and no significant difference was noted in three counts on M-PA-C at 42°C (208 CFU) , and on Cetrimide Agar at 35°C (231 CFU), at 10-6 dilution. With regards to analyzed mineral water samples, no evidence of distinct occurrence rates of P. aeruginosa on either media, at different temperatures, was noted.
Carmen Maria Ferrerluque - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial substantivity over time of chlorhexidine and Cetrimide
Journal of Endodontics, 2012Co-Authors: Pilar Baca, Maria Teresa Ariasmoliz, Pilar Junco, Francisca Castillo, Alberto Rodriguezarchilla, Carmen Maria FerrerluqueAbstract:Abstract Introduction To reach an adequate control of dentin infection and to promote success in endodontic therapy, antimicrobial irrigating solutions with confirmed substantivity are recommended. The aim of the present study was to evaluate the antimicrobial substantivity against Enterococcus faecalis of a dentin-volumetric unit exposed for 1 minute to chlorhexidine (CHX) and Cetrimide (CTR). Methods Standardized coronal dentin blocks of human molars, with and without collagen, were treated for 1 minute with 0.2% and 2% CHX and 0.2% CTR. Afterwards, they were exposed to E. faecalis suspension to determine the antimicrobial substantivity over a period of 60 days. Results were analyzed by means of Kaplan-Meier survival analysis ( P Results A direct relationship was seen between CHX concentration and survival time, and the most statistically significant results were obtained in specimens with collagen. CTR showed intermediate survival values close to those of 2% CHX. Conclusions The present study shows that 2% CHX used for 1 minute provides the longest substantivity followed by 0.2% CTR when applied to a dentin-volumetric model.
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antimicrobial activity of maleic acid and combinations of Cetrimide with chelating agents against enterococcus faecalis biofilm
Journal of Endodontics, 2010Co-Authors: Carmen Maria Ferrerluque, Maria Teresa Ariasmoliz, Maria Paloma Gonzalezrodriguez, Pilar BacaAbstract:Introduction: Chelating agents, such as EDTA or citric acid, can beincorporatedintoirrigants inorder to provide different properties in a single compound. Maleic acid has recently been proposed as an alternative irrigant to EDTA given its better smear layer removal and biocompatibility; however, its antimicrobial activity is not known. The purpose of the present study was therefore to evaluate the in vitro capacity of maleic acid in eradicating Enterococcus faecalis biofilms and to evaluate the combinations of Cetrimide with maleic acid, citric acid, and EDTA. Methods: E. faecalis biofilms were grown in the MBEC high-throughput device (Innovotech; Edmonton, Alberta, Canada) for 24 hours and exposed to the irrigating solutions for 30 seconds and 1 and2minutes.‘‘Eradication’’wasdefinedas100%bacterial kill. The Student t test was used to compare the efficacy of the irrigant combinations. Results: Maleic acid eradicated E. faecalis biofilms at a concentration of 0.88% after 30 seconds and at 0.11% after 2 minutes of contact time. When combined with 0.2% Cetrimide, it eradicated the biofilms at all three times of exposure. The combination of 0.2% Cetrimide with either 15% EDTA or 15% citric acid gave 100% bacterial kill after one minute of contact with the biofilms. Conclusions: Maleic acidshowedantimicrobial activity againstE. faecalis biofilm both alone or in association with Cetrimide from 30 seconds onward and the combination of EDTA and citric acid with Cetrimide eradicated biofilm after 1 minute of contact. (J Endod 2010;36:1673‐1675)
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eradication of enterococcus faecalis biofilms by Cetrimide and chlorhexidine
Journal of Endodontics, 2010Co-Authors: Maria Teresa Ariasmoliz, Carmen Maria Ferrerluque, Maria Paloma Gonzalezrodriguez, Mariano J Valderrama, Pilar BacaAbstract:Abstract Introduction Enterococcus faecalis is the most commonly isolated bacteria from root canals of teeth with persistent periapical periodontitis. Its ability to grow as a biofilm impedes the elimination of E. faecalis by using irrigating solutions. The purpose of this study was to assess the efficacy of Cetrimide and chlorhexidine (CHX), alone and in association, in combined and alternating form, in eradicating biofilms of E. faecalis. Methods Biofilms grown in the MBEC–high-throughput device for 24 hours were exposed to irrigating solutions for 30 seconds and 1 and 2 minutes. Eradication was defined as 100% kill of biofilm bacteria. The Student t test was used to compare the efficacy of the associations of the 2 irrigants. Results Cetrimide eradicated E. faecalis biofilms at concentrations of 0.5%, 0.0312%, and 0.0078% at 30 seconds and 1 and 2 minutes of contact time, respectively. CHX did not eradicate the biofilms at any of the concentrations (4% initial concentration) or times assayed. The association of 0.1% and 0.05% Cetrimide with any concentration of CHX, whether in combined or alternating application, effectively eradicated E. faecalis biofilms at all the contact times tested. Eradication was also achieved with 0.02% and 0.01% Cetrimide at 2 minutes. Statistical analysis revealed significantly better results with alternating rather than combined use of Cetrimide and CHX (P Conclusions The associated use of Cetrimide and CHX provided better results than their applications as single agents against E. faecalis biofilms, and the alternating application was significantly more effective than the combined mode of application.