The Experts below are selected from a list of 540564 Experts worldwide ranked by ideXlab platform
Akira Mizuno - One of the best experts on this subject based on the ideXlab platform.
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single molecule reverse transcription polymerase Chain Reaction using water in oil emulsion
Journal of Bioscience and Bioengineering, 2005Co-Authors: Michihiko Nakano, Naohito Nakai, Hirofumi Kurita, Jun Komatsu, Kazunori Takashima, Shinji Katsura, Akira MizunoAbstract:A new method based on a combination of reverse transcription polymerase Chain Reaction (RT-PCR) and a water-in-oil (W/O) emulsion was developed. Reverse transcription and initial thermal cycles were carried out in droplets of the W/O emulsion. Then, the droplets were united, followed by remaining secondary PCR cycles. This two-step method succeeded in detecting a single RNA molecule.
Kenneth H Rand - One of the best experts on this subject based on the ideXlab platform.
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a comparison of ligase Chain Reaction to polymerase Chain Reaction in the detection of chlamydia trachomatis endocervical infections
Infectious Diseases in Obstetrics & Gynecology, 1998Co-Authors: John D Davis, P K Riley, C W Peters, Kenneth H RandAbstract:OBJECTIVE: To compare the reliability of ligase Chain Reaction (LCR) to polymerase Chain Reaction (PCR) in detecting Chlamydia trachomatis endocervical infections. METHODS: We conducted a prospective study of 486 patients at risk for chlamydial infection of the endocervix. We obtained two endocervical specimens from each patient and used LCR and PCR to detect C. trachomatis. Discrepant results between the two techniques were resolved by repeat testing and by testing for the major outer membrane protein (MOMP) gene, if necessary. We determined the sensitivity, specificity, positive predictive value, and negative predictive value for each test, using concordant results or MOMP gene results as the "gold standard". RESULTS: Of the 486 patients, 42 (8.6%) had evidence of C. trachomatis infection after resolution of discrepant results. Of the 42 true positive specimens, 41 were positive by initial LCR and 38 were positive by initial PCR. Of the 444 true negative specimens, none had a positive initial LCR result, while 2 had a positive initial PCR test. Therefore, compared to the gold standard, LCR had a sensitivity of 97.6% and specificity of 100%, while PCR had a sensitivity of 90% and a specificity of 99.5%. The positive and negative predictive values of LCR were 100% and 99.8%, respectively. PCR had a positive predictive value of 95% and a negative predictive value of 99.1%. The difference in sensitivity of LCR versus PCR was not statistically significant (P = .125). CONCLUSION: LCR and PCR perform equally well in detecting C. trachomatis endocervical infections.
J M Lopezpila - One of the best experts on this subject based on the ideXlab platform.
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detection of naturally occurring enteroviruses in waters by reverse transcription polymerase Chain Reaction and hybridization
Applied and Environmental Microbiology, 1993Co-Authors: H Kopecka, S. Dubrou, Jeandidier Marechal, Jean Prevot, J M LopezpilaAbstract:Comparison in virus-seeded mineral water of three detection methods for enteroviruses, direct hybridization, cell culture, and reverse transcription into cDNA followed by polymerase Chain Reaction and hybridization, showed that the last procedure was 10 to 1,000 times more sensitive than detection by cell culture and 10(5) to 10(7) times more sensitive than direct hybridization. The presence of naturally occurring enteroviruses was also demonstrated in activated sludge and in concentrated and non-concentrated surface water samples by reverse transcription-polymerase Chain Reaction-hybridization. However, in activated sludge and in concentrated surface waters, enzymatic amplification was sometimes inhibited by contaminants.
D Y Graham - One of the best experts on this subject based on the ideXlab platform.
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diagnosis of intestinal tuberculosis by polymerase Chain Reaction on endoscopic biopsy specimens
The American Journal of Gastroenterology, 1994Co-Authors: B S Anand, F E Schneider, Fouad A K Elzaatari, Ribhi M Shawar, Jill E Clarridge, D Y GrahamAbstract:Abstract It is often difficult to establish the diagnosis of intestinal tuberculosis because of close similarities with other conditions, in particular, Crohn's disease. In the present study, we used the polymerase Chain Reaction (PCR) assay on endoscopic biopsy specimens obtained from a patient with chronic diarrhea. Positive hybridization was obtained with the Mycobacterium tuberculosis probe and the patient was treated with anti-tuberculous drugs with complete resolution of the endoscopic abnormalities. This study demonstrates that polymerase Chain Reaction assay can be used on endoscopic biopsy specimens to diagnose intestinal tuberculosis.
Michihiko Nakano - One of the best experts on this subject based on the ideXlab platform.
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single molecule reverse transcription polymerase Chain Reaction using water in oil emulsion
Journal of Bioscience and Bioengineering, 2005Co-Authors: Michihiko Nakano, Naohito Nakai, Hirofumi Kurita, Jun Komatsu, Kazunori Takashima, Shinji Katsura, Akira MizunoAbstract:A new method based on a combination of reverse transcription polymerase Chain Reaction (RT-PCR) and a water-in-oil (W/O) emulsion was developed. Reverse transcription and initial thermal cycles were carried out in droplets of the W/O emulsion. Then, the droplets were united, followed by remaining secondary PCR cycles. This two-step method succeeded in detecting a single RNA molecule.