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Wansang Lim - One of the best experts on this subject based on the ideXlab platform.

  • co expression of onion Chalcone Isomerase in del ros1 expressing tomato enhances anthocyanin and flavonol production
    Plant Cell Tissue and Organ Culture, 2017
    Co-Authors: Wansang Lim
    Abstract:

    Anthocyanins are colorful pigments known for contributing antioxidant effects to the human diet which provide health benefits that protect against several forms of cancer and vascular disease. Unfortunately, tomatoes found in nature have very low anthocyanin content. Anthocyanin-rich purple tomatoes by the ectopic co-expression of two transcription factors Delila (Del) and Rosea1 (Ros1) from the snapdragon Antirrhinum majus have been generated. However, the Del/Ros1 (DR)-expressing tomatoes cannot sufficiently upregulate all necessary key endogenous genes, particularly Chalcone Isomerase (CHI), for full utilization of the anthocyanin production pathway. In this study, CHI from onion Allium cepa was introduced into DR-expressing tomatoes for a further increase of anthocyanin levels in both the peel and the flesh of tomatoes. We achieved up to 400- and 260-fold increases in the levels of anthocyanins in tomato peel and flesh, respectively, in CHI/DR transgenics compared with 100- and 80-fold increases in tomato peel and flesh in the DR-only-expressing lines. Furthermore, CHI/DR-expressing tomatoes increased up to 200-fold more total flavonol content in flesh compared with the wild-type tomatoes. In summary, stacking CHI with DR could significantly increase the levels of anthocyanins and flavonols in tomato fruit.

  • Co-expression of onion Chalcone Isomerase in Del / Ros1 -expressing tomato enhances anthocyanin and flavonol production
    Plant Cell Tissue and Organ Culture, 2016
    Co-Authors: Wansang Lim
    Abstract:

    Anthocyanins are colorful pigments known for contributing antioxidant effects to the human diet which provide health benefits that protect against several forms of cancer and vascular disease. Unfortunately, tomatoes found in nature have very low anthocyanin content. Anthocyanin-rich purple tomatoes by the ectopic co-expression of two transcription factors Delila (Del) and Rosea1 (Ros1) from the snapdragon Antirrhinum majus have been generated. However, the Del/Ros1 (DR)-expressing tomatoes cannot sufficiently upregulate all necessary key endogenous genes, particularly Chalcone Isomerase (CHI), for full utilization of the anthocyanin production pathway. In this study, CHI from onion Allium cepa was introduced into DR-expressing tomatoes for a further increase of anthocyanin levels in both the peel and the flesh of tomatoes. We achieved up to 400- and 260-fold increases in the levels of anthocyanins in tomato peel and flesh, respectively, in CHI/DR transgenics compared with 100- and 80-fold increases in tomato peel and flesh in the DR-only-expressing lines. Furthermore, CHI/DR-expressing tomatoes increased up to 200-fold more total flavonol content in flesh compared with the wild-type tomatoes. In summary, stacking CHI with DR could significantly increase the levels of anthocyanins and flavonols in tomato fruit.

Donald Grierson - One of the best experts on this subject based on the ideXlab platform.

  • Three AP2/ERF family members modulate flavonoid synthesis by regulating type IV Chalcone Isomerase in citrus.
    Plant biotechnology journal, 2020
    Co-Authors: Chenning Zhao, Xiaojuan Liu, Qin Gong, Jinping Cao, Wanxia Shen, Xueren Yin, Donald Grierson, Bo Zhang
    Abstract:

    Flavanones and flavones are excellent source of bioactive compounds but the molecular basis of their highly efficient production remains elusive. Chalcone Isomerase (CHI) family proteins play essential roles in flavonoids biosynthesis but little are known about the transcription factors controlling their gene expression. Here, we identified a type IV CHI (designated as CitCHIL1) from citrus which enhances the accumulation of citrus flavanones and flavones (CFLs). CitCHIL1 participates in a CFLs biosynthetic metabolon and assists the cyclization of naringenin Chalcone to (2S)-naringenin, which leads to the efficient influx of substrates to Chalcone synthase (CHS) and improves the catalytic efficiency of CHS. Overexpressing CitCHIL1 in Citrus and Arabidopsis significantly increased flavonoids content and RNA interference-induced silencing of CitCHIL1 in citrus led to a 32% reduction in CFLs content. Three AP2/ERF transcription factors were identified as positive regulators of the CitCHIL1 expression. Of these, two dehydration-responsive element binding (DREB) proteins, CitERF32 and CitERF33, activated the transcription by directly binding to the CGCCGC motif in the promoter, while CitRAV1 (RAV: Related to ABI3/VP1) formed a transcription complex with CitERF33 that strongly enhanced the activation efficiency and flavonoid accumulation. These results not only illustrate the specific function that CitCHIL1 executes in CFLs biosynthesis but also reveal a new DREB-RAV transcriptional complex regulating flavonoid production.

  • three ap2 erf family members modulate flavonoid synthesis by regulating type iv Chalcone Isomerase in citrus
    Plant Biotechnology Journal, 2020
    Co-Authors: Chenning Zhao, Xiaojuan Liu, Qin Gong, Jinping Cao, Wanxia Shen, Xueren Yin, Donald Grierson, Bo Zhang, Kunsong Chen
    Abstract:

    Flavanones and flavones are excellent source of bioactive compounds but the molecular basis of their highly efficient production remains elusive. Chalcone Isomerase (CHI) family proteins play essential roles in flavonoids biosynthesis but little are known about the transcription factors controlling their gene expression. Here, we identified a type IV CHI (designated as CitCHIL1) from citrus which enhances the accumulation of citrus flavanones and flavones (CFLs). CitCHIL1 participates in a CFLs biosynthetic metabolon and assists the cyclization of naringenin Chalcone to (2S)-naringenin, which leads to the efficient influx of substrates to Chalcone synthase (CHS) and improves the catalytic efficiency of CHS. Overexpressing CitCHIL1 in Citrus and Arabidopsis significantly increased flavonoids content and RNA interference-induced silencing of CitCHIL1 in citrus led to a 32% reduction in CFLs content. Three AP2/ERF transcription factors were identified as positive regulators of the CitCHIL1 expression. Of these, two dehydration-responsive element binding (DREB) proteins, CitERF32 and CitERF33, activated the transcription by directly binding to the CGCCGC motif in the promoter, while CitRAV1 (RAV: Related to ABI3/VP1) formed a transcription complex with CitERF33 that strongly enhanced the activation efficiency and flavonoid accumulation. These results not only illustrate the specific function that CitCHIL1 executes in CFLs biosynthesis but also reveal a new DREB-RAV transcriptional complex regulating flavonoid production.

Kunsong Chen - One of the best experts on this subject based on the ideXlab platform.

  • three ap2 erf family members modulate flavonoid synthesis by regulating type iv Chalcone Isomerase in citrus
    Plant Biotechnology Journal, 2020
    Co-Authors: Chenning Zhao, Xiaojuan Liu, Qin Gong, Jinping Cao, Wanxia Shen, Xueren Yin, Donald Grierson, Bo Zhang, Kunsong Chen
    Abstract:

    Flavanones and flavones are excellent source of bioactive compounds but the molecular basis of their highly efficient production remains elusive. Chalcone Isomerase (CHI) family proteins play essential roles in flavonoids biosynthesis but little are known about the transcription factors controlling their gene expression. Here, we identified a type IV CHI (designated as CitCHIL1) from citrus which enhances the accumulation of citrus flavanones and flavones (CFLs). CitCHIL1 participates in a CFLs biosynthetic metabolon and assists the cyclization of naringenin Chalcone to (2S)-naringenin, which leads to the efficient influx of substrates to Chalcone synthase (CHS) and improves the catalytic efficiency of CHS. Overexpressing CitCHIL1 in Citrus and Arabidopsis significantly increased flavonoids content and RNA interference-induced silencing of CitCHIL1 in citrus led to a 32% reduction in CFLs content. Three AP2/ERF transcription factors were identified as positive regulators of the CitCHIL1 expression. Of these, two dehydration-responsive element binding (DREB) proteins, CitERF32 and CitERF33, activated the transcription by directly binding to the CGCCGC motif in the promoter, while CitRAV1 (RAV: Related to ABI3/VP1) formed a transcription complex with CitERF33 that strongly enhanced the activation efficiency and flavonoid accumulation. These results not only illustrate the specific function that CitCHIL1 executes in CFLs biosynthesis but also reveal a new DREB-RAV transcriptional complex regulating flavonoid production.

Yongzhen Pang - One of the best experts on this subject based on the ideXlab platform.

  • Role of a Chalcone Isomerase-like protein in flavonoid biosynthesis in Arabidopsis thaliana
    Journal of Experimental Botany, 2015
    Co-Authors: Wenbo Jiang, Qinggang Yin, Ranran Wu, Guangshun Zheng, Jinyue Liu, Richard A. Dixon, Yongzhen Pang
    Abstract:

    Flavonoids are important natural products for plant defence and human health. Although almost all the flavonoid pathway genes have been well-documented by biochemical and/or genetic approaches, the role of the Arabidopsis Chalcone Isomerase-like (CHIL) gene remains unclear. Two chil mutants with a seed colour similar to that of wild-type Arabidopsis have been identified here, but in sharp contrast to the characteristic transparent testa seed phenotype associated with other known flavonoid pathway genes. CHIL loss-of-function mutations led to a strong reduction in the proanthocyanidin and flavonol levels in seeds, but not in the anthocyanin levels in leaves. CHIL over-expression could partially recover the mutant phenotype of the chil mutant and increased both proanthocyanidin and flavonol accumulation in wild-type Arabidopsis. However, the CHIL gene could not rescue the mutant phenotype of TT5 that encodes the intrinsic Chalcone Isomerase in Arabidopsis. Parallel phenotypical and metabolic analyses of the chil, tt5, chs, and f3h mutants revealed that, genetically, CHIL functions at the same step as TT5. Moreover, it is demonstrated that CHIL co-expresses, co-localizes, and interacts with TT5 in Arabidopsis for flavonoid production. Based on these genetic and metabolic studies, it is concluded that CHIL functions with TT5 to promote flavonoid production, which is a unique enhancer in the flavonoid pathway.

Chenning Zhao - One of the best experts on this subject based on the ideXlab platform.

  • Three AP2/ERF family members modulate flavonoid synthesis by regulating type IV Chalcone Isomerase in citrus.
    Plant biotechnology journal, 2020
    Co-Authors: Chenning Zhao, Xiaojuan Liu, Qin Gong, Jinping Cao, Wanxia Shen, Xueren Yin, Donald Grierson, Bo Zhang
    Abstract:

    Flavanones and flavones are excellent source of bioactive compounds but the molecular basis of their highly efficient production remains elusive. Chalcone Isomerase (CHI) family proteins play essential roles in flavonoids biosynthesis but little are known about the transcription factors controlling their gene expression. Here, we identified a type IV CHI (designated as CitCHIL1) from citrus which enhances the accumulation of citrus flavanones and flavones (CFLs). CitCHIL1 participates in a CFLs biosynthetic metabolon and assists the cyclization of naringenin Chalcone to (2S)-naringenin, which leads to the efficient influx of substrates to Chalcone synthase (CHS) and improves the catalytic efficiency of CHS. Overexpressing CitCHIL1 in Citrus and Arabidopsis significantly increased flavonoids content and RNA interference-induced silencing of CitCHIL1 in citrus led to a 32% reduction in CFLs content. Three AP2/ERF transcription factors were identified as positive regulators of the CitCHIL1 expression. Of these, two dehydration-responsive element binding (DREB) proteins, CitERF32 and CitERF33, activated the transcription by directly binding to the CGCCGC motif in the promoter, while CitRAV1 (RAV: Related to ABI3/VP1) formed a transcription complex with CitERF33 that strongly enhanced the activation efficiency and flavonoid accumulation. These results not only illustrate the specific function that CitCHIL1 executes in CFLs biosynthesis but also reveal a new DREB-RAV transcriptional complex regulating flavonoid production.

  • three ap2 erf family members modulate flavonoid synthesis by regulating type iv Chalcone Isomerase in citrus
    Plant Biotechnology Journal, 2020
    Co-Authors: Chenning Zhao, Xiaojuan Liu, Qin Gong, Jinping Cao, Wanxia Shen, Xueren Yin, Donald Grierson, Bo Zhang, Kunsong Chen
    Abstract:

    Flavanones and flavones are excellent source of bioactive compounds but the molecular basis of their highly efficient production remains elusive. Chalcone Isomerase (CHI) family proteins play essential roles in flavonoids biosynthesis but little are known about the transcription factors controlling their gene expression. Here, we identified a type IV CHI (designated as CitCHIL1) from citrus which enhances the accumulation of citrus flavanones and flavones (CFLs). CitCHIL1 participates in a CFLs biosynthetic metabolon and assists the cyclization of naringenin Chalcone to (2S)-naringenin, which leads to the efficient influx of substrates to Chalcone synthase (CHS) and improves the catalytic efficiency of CHS. Overexpressing CitCHIL1 in Citrus and Arabidopsis significantly increased flavonoids content and RNA interference-induced silencing of CitCHIL1 in citrus led to a 32% reduction in CFLs content. Three AP2/ERF transcription factors were identified as positive regulators of the CitCHIL1 expression. Of these, two dehydration-responsive element binding (DREB) proteins, CitERF32 and CitERF33, activated the transcription by directly binding to the CGCCGC motif in the promoter, while CitRAV1 (RAV: Related to ABI3/VP1) formed a transcription complex with CitERF33 that strongly enhanced the activation efficiency and flavonoid accumulation. These results not only illustrate the specific function that CitCHIL1 executes in CFLs biosynthesis but also reveal a new DREB-RAV transcriptional complex regulating flavonoid production.