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Frank Ordon - One of the best experts on this subject based on the ideXlab platform.
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molecular analyses on the genetic diversity and inheritance of α bisabolol and Chamazulene content in tetraploid chamomile chamomilla recutita l rausch
Plant Science, 2005Co-Authors: Carola Wagner, Wolfgang Friedt, Richard A Marquard, Frank OrdonAbstract:Abstract The study aims to analyse (i) the genetic diversity of cultivated chamomile germplasm, (ii) to possibly discriminate chemotypes and (iii) to develop molecular markers for the (−)-α-bisabolol and Chamazulene content by using RAPD and AFLP marker analysis. By analysing di- and tetraploid chamomile ( Chamomilla recutita (L.) Rausch.) accessions using 18 RAPD primers and 16 Eco RI + 3/ Mse I + 3 AFLP primer combinations genetic similarity (GS D ) was estimated in a range of 0.68–0.95 (RAPDs) and 0.74–0.88 (AFLPs), respectively. UPGMA-cluster analysis revealed two main groups and additional sub-clusters according to the origin of germplasms but not to the phytochemical composition. In lines selected for the development of segregating populations for marker development a high level of genetic homogeneity within (GS D ≈ 0.9; H ′ ≈ 0.1) but diversity between lines (GS D ≈ 0.7; H ′ ≈ 0.5) was detected which facilitated the selection of contrasting parental genotypes with respect to (−)-α-bisabolol and Chamazulene content as well as genome composition. Segregation analysis of 841 F 2 -individuals revealed a monogenic recessive mode of inheritance of the (−)-α-bisabolol and Chamazulene content. By bulked segregant analysis three AFLP-markers linked to the (−)-α-bisabolol locus and one RAPD and 17 AFLP markers for the Chamazulene content were detected. At least the markers for the Chamazulene content are suited for pre-flowering marker based selection procedures.
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Molecular analyses on the genetic diversity and inheritance of (-)-α-bisabolol and Chamazulene content in tetraploid chamomile (Chamomilla recutita (L.) Rausch.)
Plant Science, 2005Co-Authors: Carola Wagner, Wolfgang Friedt, Richard A Marquard, Frank OrdonAbstract:Abstract The study aims to analyse (i) the genetic diversity of cultivated chamomile germplasm, (ii) to possibly discriminate chemotypes and (iii) to develop molecular markers for the (−)-α-bisabolol and Chamazulene content by using RAPD and AFLP marker analysis. By analysing di- and tetraploid chamomile ( Chamomilla recutita (L.) Rausch.) accessions using 18 RAPD primers and 16 Eco RI + 3/ Mse I + 3 AFLP primer combinations genetic similarity (GS D ) was estimated in a range of 0.68–0.95 (RAPDs) and 0.74–0.88 (AFLPs), respectively. UPGMA-cluster analysis revealed two main groups and additional sub-clusters according to the origin of germplasms but not to the phytochemical composition. In lines selected for the development of segregating populations for marker development a high level of genetic homogeneity within (GS D ≈ 0.9; H ′ ≈ 0.1) but diversity between lines (GS D ≈ 0.7; H ′ ≈ 0.5) was detected which facilitated the selection of contrasting parental genotypes with respect to (−)-α-bisabolol and Chamazulene content as well as genome composition. Segregation analysis of 841 F 2 -individuals revealed a monogenic recessive mode of inheritance of the (−)-α-bisabolol and Chamazulene content. By bulked segregant analysis three AFLP-markers linked to the (−)-α-bisabolol locus and one RAPD and 17 AFLP markers for the Chamazulene content were detected. At least the markers for the Chamazulene content are suited for pre-flowering marker based selection procedures.
Carola Wagner - One of the best experts on this subject based on the ideXlab platform.
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molecular analyses on the genetic diversity and inheritance of α bisabolol and Chamazulene content in tetraploid chamomile chamomilla recutita l rausch
Plant Science, 2005Co-Authors: Carola Wagner, Wolfgang Friedt, Richard A Marquard, Frank OrdonAbstract:Abstract The study aims to analyse (i) the genetic diversity of cultivated chamomile germplasm, (ii) to possibly discriminate chemotypes and (iii) to develop molecular markers for the (−)-α-bisabolol and Chamazulene content by using RAPD and AFLP marker analysis. By analysing di- and tetraploid chamomile ( Chamomilla recutita (L.) Rausch.) accessions using 18 RAPD primers and 16 Eco RI + 3/ Mse I + 3 AFLP primer combinations genetic similarity (GS D ) was estimated in a range of 0.68–0.95 (RAPDs) and 0.74–0.88 (AFLPs), respectively. UPGMA-cluster analysis revealed two main groups and additional sub-clusters according to the origin of germplasms but not to the phytochemical composition. In lines selected for the development of segregating populations for marker development a high level of genetic homogeneity within (GS D ≈ 0.9; H ′ ≈ 0.1) but diversity between lines (GS D ≈ 0.7; H ′ ≈ 0.5) was detected which facilitated the selection of contrasting parental genotypes with respect to (−)-α-bisabolol and Chamazulene content as well as genome composition. Segregation analysis of 841 F 2 -individuals revealed a monogenic recessive mode of inheritance of the (−)-α-bisabolol and Chamazulene content. By bulked segregant analysis three AFLP-markers linked to the (−)-α-bisabolol locus and one RAPD and 17 AFLP markers for the Chamazulene content were detected. At least the markers for the Chamazulene content are suited for pre-flowering marker based selection procedures.
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Molecular analyses on the genetic diversity and inheritance of (-)-α-bisabolol and Chamazulene content in tetraploid chamomile (Chamomilla recutita (L.) Rausch.)
Plant Science, 2005Co-Authors: Carola Wagner, Wolfgang Friedt, Richard A Marquard, Frank OrdonAbstract:Abstract The study aims to analyse (i) the genetic diversity of cultivated chamomile germplasm, (ii) to possibly discriminate chemotypes and (iii) to develop molecular markers for the (−)-α-bisabolol and Chamazulene content by using RAPD and AFLP marker analysis. By analysing di- and tetraploid chamomile ( Chamomilla recutita (L.) Rausch.) accessions using 18 RAPD primers and 16 Eco RI + 3/ Mse I + 3 AFLP primer combinations genetic similarity (GS D ) was estimated in a range of 0.68–0.95 (RAPDs) and 0.74–0.88 (AFLPs), respectively. UPGMA-cluster analysis revealed two main groups and additional sub-clusters according to the origin of germplasms but not to the phytochemical composition. In lines selected for the development of segregating populations for marker development a high level of genetic homogeneity within (GS D ≈ 0.9; H ′ ≈ 0.1) but diversity between lines (GS D ≈ 0.7; H ′ ≈ 0.5) was detected which facilitated the selection of contrasting parental genotypes with respect to (−)-α-bisabolol and Chamazulene content as well as genome composition. Segregation analysis of 841 F 2 -individuals revealed a monogenic recessive mode of inheritance of the (−)-α-bisabolol and Chamazulene content. By bulked segregant analysis three AFLP-markers linked to the (−)-α-bisabolol locus and one RAPD and 17 AFLP markers for the Chamazulene content were detected. At least the markers for the Chamazulene content are suited for pre-flowering marker based selection procedures.
Leila Riahi - One of the best experts on this subject based on the ideXlab platform.
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Genetic resources of Tunisian Artemisia arborescens L. (Asteraceae), pattern of volatile metabolites concentration and bioactivity and implication for conservation
Biochemical Systematics and Ecology, 2019Co-Authors: Leila Riahi, Hnia Chograni, Ahmed Masmoudi, Ameur CherifAbstract:Abstract Artemisia arborescens L. (Asteraceae) is an endemic and a valuable medicinal and ornamental species in the Mediterranean regions. Despite the interest given to A. arborescens L. as promising medicinal crop, this species is becoming threatened of extinction in Tunisia as a result of the fragmentation of its habitat and the absence of any specific measures for its conservation and valorisation. Based on the last considerations, extent information about their volatile metabolites profiling, contents and antimicrobial potential were undertaken based on fourteen populations originated from various geographical and bioclimatic habitats of Tunisia. The obtained results reveal an essential oil content mean value of 1.47%. The leaves of Tunisian tree wormwood populations were dominated by camphor (15.05–58.05%), Chamazulene (11.72–45.26%) and β-thujone (0–36.53%). Based on the three first major compounds detected for each population, four different oil types were defined (Oil type I: camphor-Chamazulene-β-thujone; Oil type II: camphor-Chamazulene-terpinen-4-ol; Oil type III: camphor-Chamazulene-bornyl acetate; Oil type IV: camphor-Chamazulene-linalool). PCA, HCA and Heatmap chemometric analyses revealed three classification clusters defined mainly by minor compounds. These minor volatile metabolites succeed to discriminate among the fourteen studied populations which promote their chemotaxonomic interest. Our findings confirm previous reports concerning this species in the world and show Chamazulene, camphor and β-thujone as the major compounds of this species which define their well known chemotypes independently of the geographical, bioclimatic and genotypic factors. This makes of these three components promising chemosystematic tool for the discrimination of A. arborescens L. in the genus Artemisia. The results showed that the four tested oils were effective against the nine tested microbial strains. The highest antimicrobial potential was revealed for the population AAr_MB characterized by camphor, Chamazulene and β-thujone as the dominant components. The pattern of essential oils concentration offers wide range of possibilities for the initiation of breeding programs according to the desired bioactive compounds. Both in situ and ex situ conservation strategies were suggested for the maintenance of the highest chemical polymorphism of this valuable medicinal species.
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effect of environmental conditions on chemical polymorphism and biological activities among artemisia absinthium l essential oil provenances grown in tunisia
Industrial Crops and Products, 2015Co-Authors: Leila Riahi, Ameur Cherif, Hanene Ghazghazi, Besma Ayari, Chedia Aouadhi, Imen Klay, Hnya Chograni, Nejia ZoghlamiAbstract:Abstract Variation on yields, chemical composition and biological activities of essential oils of Artemisia absinthium L. collected from four different bioclimatic areas ranging from Humid to Arid stage of Tunisia was investigated. The observed yields of essential oils increase significantly from arid to humid climate. A significant qualitative and quantitative variation of the chemical composition according to the studied localities was revealed. Plants collected from the Inferior Arid bioclimatic region (Gafsa) presented Chamazulene, α-thujone and camphor as the main components of their essential oils. However, for Superior Arid (Kasserine) and Semi Arid (El Kef) bioclimatic regions, camphor and Chamazulene are the dominant constituents followed by linalool for Kasserine and bornyl acetate for El Kef originated oils. The Humid bioclimatic zone (Ghar Dimaou) showed different chemotype and presented camphor, (Z)-sabinene hydrate and 1-terpinen-4-ol as the major compounds. Based on the two DPPH and ABTS tests, the investigated oils highlighted important in vitro antioxidant capacities which increase significantly from the humid (Ghar Dimaou) to the Inferior Arid bioclimatic zone (Gasfa). Even the investigated essential oils displayed an antimicrobial activity against all the tested bacterial and fungal strains with variable degrees, our findings did not reveal a clear correlation between the antimicrobial properties and the studied bioclimatic zones.
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Variations in Tunisian wormwood essential oil profiles and phenolic contents between leaves and flowers and their effects on antioxidant activities
Industrial Crops and Products, 2013Co-Authors: Leila Riahi, Hnia Chograni, Miryam Elferchichi, Yosr Zaouali, Nejia Zoghlami, Ahmed MlikiAbstract:Abstract This study is the first to investigate the chemical characterization of leaves and flowers essential oils and methanolic extract of Artemisia absinthium L. in Tunisia and their effect on the antioxidant activities. The yield of essential oil was higher in flowers (2.98%) than in leaves (1.87%). Qualitative and quantitative differences among the analyzed organ parts were revealed. 38 compounds in flower oils and 19 components in leaves oils were identified representing, respectively, 93.95% and 98.5% of the total oil composition. The two types of oils were dominated by Chamazulene (flowers: 29.9%, leaves: 30.41%) and β-thujone (flowers: 19.66%, leaves: 25.75%). Flowers oils are characterized by camphor (16.16%) whereas leaves essential oils are distinguished by bornan-2-one (17.33%). According to our results Chamazulene chemotype is probably specific to North African region for essential oil of Artemisia absinthium L. Essential oils show important antioxidant activities; however the highest antioxidant activities were recorded for methanol extract. In both cases leaves extract highlighted higher antioxidant capacity. Significant correlations were observed between the total phenols or flavonoid contents in methanol extracts and antioxidant activity estimated for both used tests even for leaves or for flowers extracts.
Wolfgang Friedt - One of the best experts on this subject based on the ideXlab platform.
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molecular analyses on the genetic diversity and inheritance of α bisabolol and Chamazulene content in tetraploid chamomile chamomilla recutita l rausch
Plant Science, 2005Co-Authors: Carola Wagner, Wolfgang Friedt, Richard A Marquard, Frank OrdonAbstract:Abstract The study aims to analyse (i) the genetic diversity of cultivated chamomile germplasm, (ii) to possibly discriminate chemotypes and (iii) to develop molecular markers for the (−)-α-bisabolol and Chamazulene content by using RAPD and AFLP marker analysis. By analysing di- and tetraploid chamomile ( Chamomilla recutita (L.) Rausch.) accessions using 18 RAPD primers and 16 Eco RI + 3/ Mse I + 3 AFLP primer combinations genetic similarity (GS D ) was estimated in a range of 0.68–0.95 (RAPDs) and 0.74–0.88 (AFLPs), respectively. UPGMA-cluster analysis revealed two main groups and additional sub-clusters according to the origin of germplasms but not to the phytochemical composition. In lines selected for the development of segregating populations for marker development a high level of genetic homogeneity within (GS D ≈ 0.9; H ′ ≈ 0.1) but diversity between lines (GS D ≈ 0.7; H ′ ≈ 0.5) was detected which facilitated the selection of contrasting parental genotypes with respect to (−)-α-bisabolol and Chamazulene content as well as genome composition. Segregation analysis of 841 F 2 -individuals revealed a monogenic recessive mode of inheritance of the (−)-α-bisabolol and Chamazulene content. By bulked segregant analysis three AFLP-markers linked to the (−)-α-bisabolol locus and one RAPD and 17 AFLP markers for the Chamazulene content were detected. At least the markers for the Chamazulene content are suited for pre-flowering marker based selection procedures.
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Molecular analyses on the genetic diversity and inheritance of (-)-α-bisabolol and Chamazulene content in tetraploid chamomile (Chamomilla recutita (L.) Rausch.)
Plant Science, 2005Co-Authors: Carola Wagner, Wolfgang Friedt, Richard A Marquard, Frank OrdonAbstract:Abstract The study aims to analyse (i) the genetic diversity of cultivated chamomile germplasm, (ii) to possibly discriminate chemotypes and (iii) to develop molecular markers for the (−)-α-bisabolol and Chamazulene content by using RAPD and AFLP marker analysis. By analysing di- and tetraploid chamomile ( Chamomilla recutita (L.) Rausch.) accessions using 18 RAPD primers and 16 Eco RI + 3/ Mse I + 3 AFLP primer combinations genetic similarity (GS D ) was estimated in a range of 0.68–0.95 (RAPDs) and 0.74–0.88 (AFLPs), respectively. UPGMA-cluster analysis revealed two main groups and additional sub-clusters according to the origin of germplasms but not to the phytochemical composition. In lines selected for the development of segregating populations for marker development a high level of genetic homogeneity within (GS D ≈ 0.9; H ′ ≈ 0.1) but diversity between lines (GS D ≈ 0.7; H ′ ≈ 0.5) was detected which facilitated the selection of contrasting parental genotypes with respect to (−)-α-bisabolol and Chamazulene content as well as genome composition. Segregation analysis of 841 F 2 -individuals revealed a monogenic recessive mode of inheritance of the (−)-α-bisabolol and Chamazulene content. By bulked segregant analysis three AFLP-markers linked to the (−)-α-bisabolol locus and one RAPD and 17 AFLP markers for the Chamazulene content were detected. At least the markers for the Chamazulene content are suited for pre-flowering marker based selection procedures.
Richard A Marquard - One of the best experts on this subject based on the ideXlab platform.
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molecular analyses on the genetic diversity and inheritance of α bisabolol and Chamazulene content in tetraploid chamomile chamomilla recutita l rausch
Plant Science, 2005Co-Authors: Carola Wagner, Wolfgang Friedt, Richard A Marquard, Frank OrdonAbstract:Abstract The study aims to analyse (i) the genetic diversity of cultivated chamomile germplasm, (ii) to possibly discriminate chemotypes and (iii) to develop molecular markers for the (−)-α-bisabolol and Chamazulene content by using RAPD and AFLP marker analysis. By analysing di- and tetraploid chamomile ( Chamomilla recutita (L.) Rausch.) accessions using 18 RAPD primers and 16 Eco RI + 3/ Mse I + 3 AFLP primer combinations genetic similarity (GS D ) was estimated in a range of 0.68–0.95 (RAPDs) and 0.74–0.88 (AFLPs), respectively. UPGMA-cluster analysis revealed two main groups and additional sub-clusters according to the origin of germplasms but not to the phytochemical composition. In lines selected for the development of segregating populations for marker development a high level of genetic homogeneity within (GS D ≈ 0.9; H ′ ≈ 0.1) but diversity between lines (GS D ≈ 0.7; H ′ ≈ 0.5) was detected which facilitated the selection of contrasting parental genotypes with respect to (−)-α-bisabolol and Chamazulene content as well as genome composition. Segregation analysis of 841 F 2 -individuals revealed a monogenic recessive mode of inheritance of the (−)-α-bisabolol and Chamazulene content. By bulked segregant analysis three AFLP-markers linked to the (−)-α-bisabolol locus and one RAPD and 17 AFLP markers for the Chamazulene content were detected. At least the markers for the Chamazulene content are suited for pre-flowering marker based selection procedures.
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Molecular analyses on the genetic diversity and inheritance of (-)-α-bisabolol and Chamazulene content in tetraploid chamomile (Chamomilla recutita (L.) Rausch.)
Plant Science, 2005Co-Authors: Carola Wagner, Wolfgang Friedt, Richard A Marquard, Frank OrdonAbstract:Abstract The study aims to analyse (i) the genetic diversity of cultivated chamomile germplasm, (ii) to possibly discriminate chemotypes and (iii) to develop molecular markers for the (−)-α-bisabolol and Chamazulene content by using RAPD and AFLP marker analysis. By analysing di- and tetraploid chamomile ( Chamomilla recutita (L.) Rausch.) accessions using 18 RAPD primers and 16 Eco RI + 3/ Mse I + 3 AFLP primer combinations genetic similarity (GS D ) was estimated in a range of 0.68–0.95 (RAPDs) and 0.74–0.88 (AFLPs), respectively. UPGMA-cluster analysis revealed two main groups and additional sub-clusters according to the origin of germplasms but not to the phytochemical composition. In lines selected for the development of segregating populations for marker development a high level of genetic homogeneity within (GS D ≈ 0.9; H ′ ≈ 0.1) but diversity between lines (GS D ≈ 0.7; H ′ ≈ 0.5) was detected which facilitated the selection of contrasting parental genotypes with respect to (−)-α-bisabolol and Chamazulene content as well as genome composition. Segregation analysis of 841 F 2 -individuals revealed a monogenic recessive mode of inheritance of the (−)-α-bisabolol and Chamazulene content. By bulked segregant analysis three AFLP-markers linked to the (−)-α-bisabolol locus and one RAPD and 17 AFLP markers for the Chamazulene content were detected. At least the markers for the Chamazulene content are suited for pre-flowering marker based selection procedures.