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Richard H. Furneaux - One of the best experts on this subject based on the ideXlab platform.
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A polysaccharide fraction from the red seaweed Champia novae-zealandiae Rhodymeniales, Rhodophyta
Hydrobiologia, 1996Co-Authors: Ian J. Miller, Ruth Falshaw, Richard H. FurneauxAbstract:The polysaccharide recovered after extraction of Champia novae-zealandiae is a galactan with alternating 3-linked d -galactopyranosyl units sulfated at the 2-position, and 4-linked galactopyranosyl units sulfated at both the 2- and 3-positions that are predominantly of the l - and partly of the d -configuration. Other minor substitution includes 6- O -methyl ether or 4,6-pyruvate acetal on the 3-linked residues. Techniques used in determining the structure include infrared and ^13C-NMR spectroscopy, and GC-MS analysis of alditol acetate derivatives produced by reductive hydrolysis/acetylation of native, methylated, and/or desulfated samples. These results are of particular interest because 4-linked 2,3-desulfated galactosyl residues have not been encountered as major constituents of red algal polysaccharides.
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A polysaccharide fraction from the red seaweed Champia novae-zealandiae Rhodymeniales, Rhodophyta
Fifteenth International Seaweed Symposium, 1996Co-Authors: Ian J. Miller, Ruth Falshaw, Richard H. FurneauxAbstract:The polysaccharide recovered after extraction of Champia novae-zealandiae is a galactan with alternating 3-linked D-galactopyranosyl units sulfated at the 2-position, and 4-linked galactopyranosyl units sulfated at both the 2- and 3-positions that are predominantly of the L- and partly of the D-configuration. Other minor substitution includes 6-O-methyl ether or 4,6-pyruvate acetal on the 3-linked residues. Techniques used in determining the structure include infrared and 13C-NMR spectroscopy, and GC-MS analysis of alditol acetate derivatives produced by reductive hydrolysis/acetylation of native, methylated, and/or desulfated samples. These results are of particular interest because 4-linked 2,3-disulfated galactosyl residues have not been encountered as major constituents of red algal polysaccharides.
Ian J. Miller - One of the best experts on this subject based on the ideXlab platform.
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A polysaccharide fraction from the red seaweed Champia novae-zealandiae Rhodymeniales, Rhodophyta
Hydrobiologia, 1996Co-Authors: Ian J. Miller, Ruth Falshaw, Richard H. FurneauxAbstract:The polysaccharide recovered after extraction of Champia novae-zealandiae is a galactan with alternating 3-linked d -galactopyranosyl units sulfated at the 2-position, and 4-linked galactopyranosyl units sulfated at both the 2- and 3-positions that are predominantly of the l - and partly of the d -configuration. Other minor substitution includes 6- O -methyl ether or 4,6-pyruvate acetal on the 3-linked residues. Techniques used in determining the structure include infrared and ^13C-NMR spectroscopy, and GC-MS analysis of alditol acetate derivatives produced by reductive hydrolysis/acetylation of native, methylated, and/or desulfated samples. These results are of particular interest because 4-linked 2,3-desulfated galactosyl residues have not been encountered as major constituents of red algal polysaccharides.
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A polysaccharide fraction from the red seaweed Champia novae-zealandiae Rhodymeniales, Rhodophyta
Fifteenth International Seaweed Symposium, 1996Co-Authors: Ian J. Miller, Ruth Falshaw, Richard H. FurneauxAbstract:The polysaccharide recovered after extraction of Champia novae-zealandiae is a galactan with alternating 3-linked D-galactopyranosyl units sulfated at the 2-position, and 4-linked galactopyranosyl units sulfated at both the 2- and 3-positions that are predominantly of the L- and partly of the D-configuration. Other minor substitution includes 6-O-methyl ether or 4,6-pyruvate acetal on the 3-linked residues. Techniques used in determining the structure include infrared and 13C-NMR spectroscopy, and GC-MS analysis of alditol acetate derivatives produced by reductive hydrolysis/acetylation of native, methylated, and/or desulfated samples. These results are of particular interest because 4-linked 2,3-disulfated galactosyl residues have not been encountered as major constituents of red algal polysaccharides.
Kézia O.a.l. Lins - One of the best experts on this subject based on the ideXlab platform.
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Antitumor properties of a sulfated polysaccharide from the red seaweed Champia feldmannii (Diaz‐Pifferer)
Journal of applied toxicology : JAT, 2009Co-Authors: Kézia O.a.l. Lins, Daniel P. Bezerra, Ana Paula Negreiros Nunes Alves, Nylane M.n. Alencar, Michael W. Lima, Valeska Martins Torres, Wladimir Ronald Lobo Farias, Cláudia Pessoa, Manoel Odorico De Moraes, Letícia V. Costa-lotufoAbstract:In recent years, much attention has been focused on polysaccharides isolated from natural sources. The aim of this study was to investigate the in vitro and in vivo antitumor properties of a sulfated polysaccharide isolated from the seaweed C. feldmannii (Cf-PLS). Hematological, biochemical and histopathological analyses were performed in order to evaluate the toxicological aspects related to Cf-PLS treatment. Its effects on the immunological system were also investigated. The Cf-PLS did not show any significant in vitro cytotoxicity at the experimental exposure levels that were used, but showed in vivo antitumor effect. The inhibition rates of sarcoma 180 tumor development were 48.62 and 48.16% at the doses of 10 and 25 mg kg−1, respectively. In addition, Cf-PLS was also able to increase the response elicited by 5-fluorouracil (5-FU) from 48.66 to 68.32%. The histopathological analysis of liver and kidney showed that both organs were moderately affected by Cf-PLS-treatment. Neither enzymatic activity of alanine aminotransferase nor urea or creatinine levels were significantly altered. In hematological analysis, leucopeny was observed after 5-FU treatment, but this effect was prevented when the treatment was associated with the Cf-PLS. It was also demonstrated that Cf-PLS acts as an immunomodulatory agent, raising the production of specific antibodies, and increasing the production of OVA-specific antibodies. It also induced a discreet hyperplasia of lymphoid folicules of the white pulp in the spleen of treated mice. In conclusion, Cf-PLS has some interesting anticancer activity that could be associated with its immunostimulating properties. Copyright © 2008 John Wiley & Sons, Ltd.
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antitumor properties of a sulfated polysaccharide from the red seaweed Champia feldmannii diaz pifferer
Journal of Applied Toxicology, 2009Co-Authors: Kézia O.a.l. Lins, Daniel P. Bezerra, Ana Paula Negreiros Nunes Alves, Nylane M.n. Alencar, Michael W. Lima, Valeska Martins Torres, Wladimir Ronald Lobo Farias, Cláudia Pessoa, Manoel Odorico De Moraes, Leticia V CostalotufoAbstract:In recent years, much attention has been focused on polysaccharides isolated from natural sources. The aim of this study was to investigate the in vitro and in vivo antitumor properties of a sulfated polysaccharide isolated from the seaweed C. feldmannii (Cf-PLS). Hematological, biochemical and histopathological analyses were performed in order to evaluate the toxicological aspects related to Cf-PLS treatment. Its effects on the immunological system were also investigated. The Cf-PLS did not show any significant in vitro cytotoxicity at the experimental exposure levels that were used, but showed in vivo antitumor effect. The inhibition rates of sarcoma 180 tumor development were 48.62 and 48.16% at the doses of 10 and 25 mg kg−1, respectively. In addition, Cf-PLS was also able to increase the response elicited by 5-fluorouracil (5-FU) from 48.66 to 68.32%. The histopathological analysis of liver and kidney showed that both organs were moderately affected by Cf-PLS-treatment. Neither enzymatic activity of alanine aminotransferase nor urea or creatinine levels were significantly altered. In hematological analysis, leucopeny was observed after 5-FU treatment, but this effect was prevented when the treatment was associated with the Cf-PLS. It was also demonstrated that Cf-PLS acts as an immunomodulatory agent, raising the production of specific antibodies, and increasing the production of OVA-specific antibodies. It also induced a discreet hyperplasia of lymphoid folicules of the white pulp in the spleen of treated mice. In conclusion, Cf-PLS has some interesting anticancer activity that could be associated with its immunostimulating properties. Copyright © 2008 John Wiley & Sons, Ltd.
Valeska Martins Torres - One of the best experts on this subject based on the ideXlab platform.
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Antitumor properties of a sulfated polysaccharide from the red seaweed Champia feldmannii (Diaz‐Pifferer)
Journal of applied toxicology : JAT, 2009Co-Authors: Kézia O.a.l. Lins, Daniel P. Bezerra, Ana Paula Negreiros Nunes Alves, Nylane M.n. Alencar, Michael W. Lima, Valeska Martins Torres, Wladimir Ronald Lobo Farias, Cláudia Pessoa, Manoel Odorico De Moraes, Letícia V. Costa-lotufoAbstract:In recent years, much attention has been focused on polysaccharides isolated from natural sources. The aim of this study was to investigate the in vitro and in vivo antitumor properties of a sulfated polysaccharide isolated from the seaweed C. feldmannii (Cf-PLS). Hematological, biochemical and histopathological analyses were performed in order to evaluate the toxicological aspects related to Cf-PLS treatment. Its effects on the immunological system were also investigated. The Cf-PLS did not show any significant in vitro cytotoxicity at the experimental exposure levels that were used, but showed in vivo antitumor effect. The inhibition rates of sarcoma 180 tumor development were 48.62 and 48.16% at the doses of 10 and 25 mg kg−1, respectively. In addition, Cf-PLS was also able to increase the response elicited by 5-fluorouracil (5-FU) from 48.66 to 68.32%. The histopathological analysis of liver and kidney showed that both organs were moderately affected by Cf-PLS-treatment. Neither enzymatic activity of alanine aminotransferase nor urea or creatinine levels were significantly altered. In hematological analysis, leucopeny was observed after 5-FU treatment, but this effect was prevented when the treatment was associated with the Cf-PLS. It was also demonstrated that Cf-PLS acts as an immunomodulatory agent, raising the production of specific antibodies, and increasing the production of OVA-specific antibodies. It also induced a discreet hyperplasia of lymphoid folicules of the white pulp in the spleen of treated mice. In conclusion, Cf-PLS has some interesting anticancer activity that could be associated with its immunostimulating properties. Copyright © 2008 John Wiley & Sons, Ltd.
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antitumor properties of a sulfated polysaccharide from the red seaweed Champia feldmannii diaz pifferer
Journal of Applied Toxicology, 2009Co-Authors: Kézia O.a.l. Lins, Daniel P. Bezerra, Ana Paula Negreiros Nunes Alves, Nylane M.n. Alencar, Michael W. Lima, Valeska Martins Torres, Wladimir Ronald Lobo Farias, Cláudia Pessoa, Manoel Odorico De Moraes, Leticia V CostalotufoAbstract:In recent years, much attention has been focused on polysaccharides isolated from natural sources. The aim of this study was to investigate the in vitro and in vivo antitumor properties of a sulfated polysaccharide isolated from the seaweed C. feldmannii (Cf-PLS). Hematological, biochemical and histopathological analyses were performed in order to evaluate the toxicological aspects related to Cf-PLS treatment. Its effects on the immunological system were also investigated. The Cf-PLS did not show any significant in vitro cytotoxicity at the experimental exposure levels that were used, but showed in vivo antitumor effect. The inhibition rates of sarcoma 180 tumor development were 48.62 and 48.16% at the doses of 10 and 25 mg kg−1, respectively. In addition, Cf-PLS was also able to increase the response elicited by 5-fluorouracil (5-FU) from 48.66 to 68.32%. The histopathological analysis of liver and kidney showed that both organs were moderately affected by Cf-PLS-treatment. Neither enzymatic activity of alanine aminotransferase nor urea or creatinine levels were significantly altered. In hematological analysis, leucopeny was observed after 5-FU treatment, but this effect was prevented when the treatment was associated with the Cf-PLS. It was also demonstrated that Cf-PLS acts as an immunomodulatory agent, raising the production of specific antibodies, and increasing the production of OVA-specific antibodies. It also induced a discreet hyperplasia of lymphoid folicules of the white pulp in the spleen of treated mice. In conclusion, Cf-PLS has some interesting anticancer activity that could be associated with its immunostimulating properties. Copyright © 2008 John Wiley & Sons, Ltd.
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Biological effects of a sulfated-polysaccharide isolated from the marine red algae Champia feldmannii.
Biological & pharmaceutical bulletin, 2008Co-Authors: Ana Maria Sampaio Assreuy, Nylane M.n. Alencar, Valeska Martins Torres, Daniel Magalhães Gomes, Michelle Soares Josino Da Silva, Rômmulo Celly Lima Siqueira, Alana De Freitas Pires, David N. Criddle, Benildo Sousa Cavada, Alexandre Holanda SampaioAbstract:Sulfated-polysaccharides are exploited as antithrombotic and anticoagulant agents and suggested to be immunostimulants. The sulfated-polysaccharide isolated from the red-marine-algae Champia feldmannii (Cf-PLS) was purified by ion exchange chromatography and tested in experimental protocols of coagulation, inflammation (in Wistar rats) and nociception (in Swiss mice). Cf-PLS was tested i.v. for its anti-inflammatory activity in the paw-edema induced by classical inflammatory stimuli and s.c. for its pro-inflammatory activity in the paw-edema and peritonitis models. The anticoagulant activity was evaluated by the test of partial thromboplastin activation time (aPTT) and the antinociceptive effect in the writhing-test. Cf-PLS was not anti-inflammatory, but rather induced maximal edematogenic activity at 0.9 mg/kg (1.01±0.030×0.06±0.03 ml) compared to controls (0.06±0.03 ml), increased vascular-permeability (38.44±12.63×11.29±3.91 μg/g) and stimulated neutrophil migration (3.348±295×307±99 cells/μl) 1 h after injection. Cf-PLS was also antinociceptive (6.6±1.28×33±1.44 writhes) and extended human plasma coagulation time by 3 times. Our data suggest that this molecule may be an important immunostimulant.
Nylane M.n. Alencar - One of the best experts on this subject based on the ideXlab platform.
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Antitumor properties of a sulfated polysaccharide from the red seaweed Champia feldmannii (Diaz‐Pifferer)
Journal of applied toxicology : JAT, 2009Co-Authors: Kézia O.a.l. Lins, Daniel P. Bezerra, Ana Paula Negreiros Nunes Alves, Nylane M.n. Alencar, Michael W. Lima, Valeska Martins Torres, Wladimir Ronald Lobo Farias, Cláudia Pessoa, Manoel Odorico De Moraes, Letícia V. Costa-lotufoAbstract:In recent years, much attention has been focused on polysaccharides isolated from natural sources. The aim of this study was to investigate the in vitro and in vivo antitumor properties of a sulfated polysaccharide isolated from the seaweed C. feldmannii (Cf-PLS). Hematological, biochemical and histopathological analyses were performed in order to evaluate the toxicological aspects related to Cf-PLS treatment. Its effects on the immunological system were also investigated. The Cf-PLS did not show any significant in vitro cytotoxicity at the experimental exposure levels that were used, but showed in vivo antitumor effect. The inhibition rates of sarcoma 180 tumor development were 48.62 and 48.16% at the doses of 10 and 25 mg kg−1, respectively. In addition, Cf-PLS was also able to increase the response elicited by 5-fluorouracil (5-FU) from 48.66 to 68.32%. The histopathological analysis of liver and kidney showed that both organs were moderately affected by Cf-PLS-treatment. Neither enzymatic activity of alanine aminotransferase nor urea or creatinine levels were significantly altered. In hematological analysis, leucopeny was observed after 5-FU treatment, but this effect was prevented when the treatment was associated with the Cf-PLS. It was also demonstrated that Cf-PLS acts as an immunomodulatory agent, raising the production of specific antibodies, and increasing the production of OVA-specific antibodies. It also induced a discreet hyperplasia of lymphoid folicules of the white pulp in the spleen of treated mice. In conclusion, Cf-PLS has some interesting anticancer activity that could be associated with its immunostimulating properties. Copyright © 2008 John Wiley & Sons, Ltd.
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antitumor properties of a sulfated polysaccharide from the red seaweed Champia feldmannii diaz pifferer
Journal of Applied Toxicology, 2009Co-Authors: Kézia O.a.l. Lins, Daniel P. Bezerra, Ana Paula Negreiros Nunes Alves, Nylane M.n. Alencar, Michael W. Lima, Valeska Martins Torres, Wladimir Ronald Lobo Farias, Cláudia Pessoa, Manoel Odorico De Moraes, Leticia V CostalotufoAbstract:In recent years, much attention has been focused on polysaccharides isolated from natural sources. The aim of this study was to investigate the in vitro and in vivo antitumor properties of a sulfated polysaccharide isolated from the seaweed C. feldmannii (Cf-PLS). Hematological, biochemical and histopathological analyses were performed in order to evaluate the toxicological aspects related to Cf-PLS treatment. Its effects on the immunological system were also investigated. The Cf-PLS did not show any significant in vitro cytotoxicity at the experimental exposure levels that were used, but showed in vivo antitumor effect. The inhibition rates of sarcoma 180 tumor development were 48.62 and 48.16% at the doses of 10 and 25 mg kg−1, respectively. In addition, Cf-PLS was also able to increase the response elicited by 5-fluorouracil (5-FU) from 48.66 to 68.32%. The histopathological analysis of liver and kidney showed that both organs were moderately affected by Cf-PLS-treatment. Neither enzymatic activity of alanine aminotransferase nor urea or creatinine levels were significantly altered. In hematological analysis, leucopeny was observed after 5-FU treatment, but this effect was prevented when the treatment was associated with the Cf-PLS. It was also demonstrated that Cf-PLS acts as an immunomodulatory agent, raising the production of specific antibodies, and increasing the production of OVA-specific antibodies. It also induced a discreet hyperplasia of lymphoid folicules of the white pulp in the spleen of treated mice. In conclusion, Cf-PLS has some interesting anticancer activity that could be associated with its immunostimulating properties. Copyright © 2008 John Wiley & Sons, Ltd.
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Biological effects of a sulfated-polysaccharide isolated from the marine red algae Champia feldmannii.
Biological & pharmaceutical bulletin, 2008Co-Authors: Ana Maria Sampaio Assreuy, Nylane M.n. Alencar, Valeska Martins Torres, Daniel Magalhães Gomes, Michelle Soares Josino Da Silva, Rômmulo Celly Lima Siqueira, Alana De Freitas Pires, David N. Criddle, Benildo Sousa Cavada, Alexandre Holanda SampaioAbstract:Sulfated-polysaccharides are exploited as antithrombotic and anticoagulant agents and suggested to be immunostimulants. The sulfated-polysaccharide isolated from the red-marine-algae Champia feldmannii (Cf-PLS) was purified by ion exchange chromatography and tested in experimental protocols of coagulation, inflammation (in Wistar rats) and nociception (in Swiss mice). Cf-PLS was tested i.v. for its anti-inflammatory activity in the paw-edema induced by classical inflammatory stimuli and s.c. for its pro-inflammatory activity in the paw-edema and peritonitis models. The anticoagulant activity was evaluated by the test of partial thromboplastin activation time (aPTT) and the antinociceptive effect in the writhing-test. Cf-PLS was not anti-inflammatory, but rather induced maximal edematogenic activity at 0.9 mg/kg (1.01±0.030×0.06±0.03 ml) compared to controls (0.06±0.03 ml), increased vascular-permeability (38.44±12.63×11.29±3.91 μg/g) and stimulated neutrophil migration (3.348±295×307±99 cells/μl) 1 h after injection. Cf-PLS was also antinociceptive (6.6±1.28×33±1.44 writhes) and extended human plasma coagulation time by 3 times. Our data suggest that this molecule may be an important immunostimulant.