The Experts below are selected from a list of 324 Experts worldwide ranked by ideXlab platform
I Yang - One of the best experts on this subject based on the ideXlab platform.
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analysis of treponema pallidum strains from china using improved methods for whole genome sequencing from primary syphilis Chancres
The Journal of Infectious Diseases, 2020Co-Authors: Wentao Che, David Smajs, Petra Pospíšilová, Joseph D Tucke, Arlene C Sena, Kelly L Hawley, Melissa J Caimano, Justin D Radolf, I YangAbstract:Whole-genome sequencing (WGS) of Treponema pallidum subsp. pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis Chancre swabs collected in Guangzhou, China. By combining parallel, pooled whole-genome amplification (ppWGA) with hybrid selection, we generated high quality genomes from four of eight Chancre-swab samples and two of two rabbit-passaged isolates, all subjected to challenging storage conditions. This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
Joseph D Tucke - One of the best experts on this subject based on the ideXlab platform.
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analysis of treponema pallidum strains from china using improved methods for whole genome sequencing from primary syphilis Chancres
The Journal of Infectious Diseases, 2020Co-Authors: Wentao Che, David Smajs, Petra Pospíšilová, Joseph D Tucke, Arlene C Sena, Kelly L Hawley, Melissa J Caimano, Justin D Radolf, I YangAbstract:Whole-genome sequencing (WGS) of Treponema pallidum subsp. pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis Chancre swabs collected in Guangzhou, China. By combining parallel, pooled whole-genome amplification (ppWGA) with hybrid selection, we generated high quality genomes from four of eight Chancre-swab samples and two of two rabbit-passaged isolates, all subjected to challenging storage conditions. This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
M. Le Bras - One of the best experts on this subject based on the ideXlab platform.
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Human West African trypanosomiasis with Chancre presentation.
European Journal of Dermatology, 2000Co-Authors: Denis Malvy, P. Chapuis, Felix Djossou, M. Longy-boursier, François-xavier Weill, M. Le BrasAbstract:In this case, evaluation of a peripherical blood smear confirmed the diagnosis and showed trypanosomes. Indeed, the patient had two trypanosomal Chancres and examination of blood specimens revealed West African trypanosomiasis due to Trypanosoma brucei. Diagnosis of West African trypanosomiasis at the very early hemolymphatic stage was retained, and the patient received a regimen of five doses of iv pentamidine isethionate (4 mg/kg) over 10 [...]
Bin Yang - One of the best experts on this subject based on the ideXlab platform.
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analysis of treponema pallidum strains from china using improved methods for whole genome sequencing from primary syphilis Chancres
The Journal of Infectious Diseases, 2021Co-Authors: Wentao Chen, David Smajs, Petra Pospíšilová, Arlene C Sena, Kelly L Hawley, Melissa J Caimano, Justin D Radolf, Joseph D Tucker, Bin YangAbstract:BACKGROUND Whole-genome sequencing (WGS) of Treponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. METHODS We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis Chancre swabs collected in Guangzhou, China. RESULTS By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 Chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. CONCLUSIONS This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
Joseph D Tucker - One of the best experts on this subject based on the ideXlab platform.
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analysis of treponema pallidum strains from china using improved methods for whole genome sequencing from primary syphilis Chancres
The Journal of Infectious Diseases, 2021Co-Authors: Wentao Chen, David Smajs, Petra Pospíšilová, Arlene C Sena, Kelly L Hawley, Melissa J Caimano, Justin D Radolf, Joseph D Tucker, Bin YangAbstract:BACKGROUND Whole-genome sequencing (WGS) of Treponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. METHODS We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis Chancre swabs collected in Guangzhou, China. RESULTS By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 Chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. CONCLUSIONS This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.