The Experts below are selected from a list of 9369 Experts worldwide ranked by ideXlab platform
Miranda G S Yap - One of the best experts on this subject based on the ideXlab platform.
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Vector fragmentation Characterizing Vector integrity in transfected clones by southern blotting
Biotechnology Progress, 2010Co-Authors: Wenyu Lin, Rohit Sachdeva, Daniel I C Wang, Miranda G S YapAbstract:The Chinese Hamster Ovary production cell line development process using methotrexate (MTX) amplification is well studied and commonly used for biopharmaceutical processes. However, successful MTX amplification varies from clone to clone and suggested reasons include Vector fragmentation during the transfection process and genomic rearrangement of the Chinese Hamster Ovary chromosomes. Here, we elucidated the Vector integration patterns of 40 transfected single-cell clones by Southern blotting and showed that Vector fragmentation occurs at a significant level in our experiment. This concurs with MTX amplification studies implying that single-cell cloning is necessary to ensure a successful amplification process. Truncations at the ends of the integrated Vectors were also observed, whereas gross DNA insertions were not detected in our data. This suggests that end deletions are common, whereas insertion events are rare in animal cells.
Etienne Brasselet - One of the best experts on this subject based on the ideXlab platform.
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polarization singularities and mobius strips in sound and water surface waves
Physics of Fluids, 2021Co-Authors: Konstantin Y Bliokh, Miguel A Alonso, Danica Sugic, Mathias Perrin, Franco Nori, Etienne BrasseletAbstract:We show that polarization singularities, generic for any complex Vector field but so far mostly studied for electromagnetic fields, appear naturally in inhomogeneous yet monochromatic sound and water-surface (e.g., gravity or capillary) wave fields in fluids or gases. The Vector properties of these waves are described by the velocity or displacement fields Characterizing the local oscillatory motion of the medium particles. We consider a number of examples revealing C-points of purely circular polarization and polarization Mobius strips (formed by major axes of polarization ellipses) around the C-points in sound and gravity wave fields. Our results (i) offer a new readily accessible platform for studies of polarization singularities and topological features of complex Vector wave fields and (ii) can play an important role in Characterizing Vector (e.g., dipole) wave–matter interactions in acoustics and fluid mechanics.
Miguel A Alonso - One of the best experts on this subject based on the ideXlab platform.
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polarization singularities and mobius strips in sound and water surface waves
Physics of Fluids, 2021Co-Authors: Konstantin Y Bliokh, Miguel A Alonso, Danica Sugic, Mathias Perrin, Franco Nori, Etienne BrasseletAbstract:We show that polarization singularities, generic for any complex Vector field but so far mostly studied for electromagnetic fields, appear naturally in inhomogeneous yet monochromatic sound and water-surface (e.g., gravity or capillary) wave fields in fluids or gases. The Vector properties of these waves are described by the velocity or displacement fields Characterizing the local oscillatory motion of the medium particles. We consider a number of examples revealing C-points of purely circular polarization and polarization Mobius strips (formed by major axes of polarization ellipses) around the C-points in sound and gravity wave fields. Our results (i) offer a new readily accessible platform for studies of polarization singularities and topological features of complex Vector wave fields and (ii) can play an important role in Characterizing Vector (e.g., dipole) wave–matter interactions in acoustics and fluid mechanics.
Daniel I C Wang - One of the best experts on this subject based on the ideXlab platform.
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Vector fragmentation Characterizing Vector integrity in transfected clones by southern blotting
Biotechnology Progress, 2010Co-Authors: Wenyu Lin, Rohit Sachdeva, Daniel I C Wang, Miranda G S YapAbstract:The Chinese Hamster Ovary production cell line development process using methotrexate (MTX) amplification is well studied and commonly used for biopharmaceutical processes. However, successful MTX amplification varies from clone to clone and suggested reasons include Vector fragmentation during the transfection process and genomic rearrangement of the Chinese Hamster Ovary chromosomes. Here, we elucidated the Vector integration patterns of 40 transfected single-cell clones by Southern blotting and showed that Vector fragmentation occurs at a significant level in our experiment. This concurs with MTX amplification studies implying that single-cell cloning is necessary to ensure a successful amplification process. Truncations at the ends of the integrated Vectors were also observed, whereas gross DNA insertions were not detected in our data. This suggests that end deletions are common, whereas insertion events are rare in animal cells.
Konstantin Y Bliokh - One of the best experts on this subject based on the ideXlab platform.
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polarization singularities and mobius strips in sound and water surface waves
Physics of Fluids, 2021Co-Authors: Konstantin Y Bliokh, Miguel A Alonso, Danica Sugic, Mathias Perrin, Franco Nori, Etienne BrasseletAbstract:We show that polarization singularities, generic for any complex Vector field but so far mostly studied for electromagnetic fields, appear naturally in inhomogeneous yet monochromatic sound and water-surface (e.g., gravity or capillary) wave fields in fluids or gases. The Vector properties of these waves are described by the velocity or displacement fields Characterizing the local oscillatory motion of the medium particles. We consider a number of examples revealing C-points of purely circular polarization and polarization Mobius strips (formed by major axes of polarization ellipses) around the C-points in sound and gravity wave fields. Our results (i) offer a new readily accessible platform for studies of polarization singularities and topological features of complex Vector wave fields and (ii) can play an important role in Characterizing Vector (e.g., dipole) wave–matter interactions in acoustics and fluid mechanics.