The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform

Peter Myers - One of the best experts on this subject based on the ideXlab platform.

  • Quantitative ultraviolet measurements on wetted thin-layer chromatography plates using a Charge-Coupled Device Camera.
    Journal of Chromatography A, 2008
    Co-Authors: Michael Lancaster, David M. Goodall, Edmund T. Bergström, Sean Mccrossen, Peter Myers
    Abstract:

    Abstract This paper presents the first study of the UV imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging of spots of benzophenone during and after development was carried out using a Charge-Coupled Device Camera. Limits of detection were found to be 5 ng on a wetted plate and 3 ng for a dry plate and the relationship between peak area and sample loading was found to be linear in the low nanogram range over an order of magnitude for both wet and dry modes with r2 values > 0.99. It was found that UV measurements on wet glass-backed plates suffer from low sensitivity; however, the use of aluminium-backed plates gave increased sensitivity. The apparent absorption coefficient (ɛapp) of 10 AU m2 g−1 at 254 nm is consistent with reflection of the light from the aluminium surface with a double pass through the sorbent layer, and suggests that use of aluminium-backed plates should enable monitoring of separations by UV absorbance during TLC development.

  • Quantitative measurements on wetted thin layer chromatography plates using a charge coupled Device Camera.
    Journal of chromatography. A, 2005
    Co-Authors: Michael Lancaster, David M. Goodall, Edmund T. Bergström, Sean Mccrossen, Peter Myers
    Abstract:

    This paper presents the first study of imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging and quantification of Sudan II after development with dichloromethane was carried out in both reflectance and transmission modes, using a charge coupled Device (CCD) Camera. The relationship between peak area and sample loading was established at low sample loading, and found to be linear over an order of magnitude for both wet and dry modes with r2-values > 0.99. All data processing was carried out using the Beer-Lambert equation. Curvature at high loadings in the plots of integrated absorbance as a function of sample loading was accounted for using an empirical expression designed for use with the Kubelka-Munk treatment and apparent absorbance of the stationary phase due to scattering. Results are consistent with an effective pathlength significantly longer than the thickness of the sorbent layer. The limit of detection on a dry plate (0.5 ng) was found to be lower than on a wetted plate (2 ng). Precision was found to be 1-4% RSD intra-plate and 8-14% RSD inter-plate. Results are compared with quantification of the same analyte on dried plates.

Michael Lancaster - One of the best experts on this subject based on the ideXlab platform.

  • Quantitative ultraviolet measurements on wetted thin-layer chromatography plates using a Charge-Coupled Device Camera.
    Journal of Chromatography A, 2008
    Co-Authors: Michael Lancaster, David M. Goodall, Edmund T. Bergström, Sean Mccrossen, Peter Myers
    Abstract:

    Abstract This paper presents the first study of the UV imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging of spots of benzophenone during and after development was carried out using a Charge-Coupled Device Camera. Limits of detection were found to be 5 ng on a wetted plate and 3 ng for a dry plate and the relationship between peak area and sample loading was found to be linear in the low nanogram range over an order of magnitude for both wet and dry modes with r2 values > 0.99. It was found that UV measurements on wet glass-backed plates suffer from low sensitivity; however, the use of aluminium-backed plates gave increased sensitivity. The apparent absorption coefficient (ɛapp) of 10 AU m2 g−1 at 254 nm is consistent with reflection of the light from the aluminium surface with a double pass through the sorbent layer, and suggests that use of aluminium-backed plates should enable monitoring of separations by UV absorbance during TLC development.

  • Quantitative measurements on wetted thin layer chromatography plates using a charge coupled Device Camera.
    Journal of chromatography. A, 2005
    Co-Authors: Michael Lancaster, David M. Goodall, Edmund T. Bergström, Sean Mccrossen, Peter Myers
    Abstract:

    This paper presents the first study of imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging and quantification of Sudan II after development with dichloromethane was carried out in both reflectance and transmission modes, using a charge coupled Device (CCD) Camera. The relationship between peak area and sample loading was established at low sample loading, and found to be linear over an order of magnitude for both wet and dry modes with r2-values > 0.99. All data processing was carried out using the Beer-Lambert equation. Curvature at high loadings in the plots of integrated absorbance as a function of sample loading was accounted for using an empirical expression designed for use with the Kubelka-Munk treatment and apparent absorbance of the stationary phase due to scattering. Results are consistent with an effective pathlength significantly longer than the thickness of the sorbent layer. The limit of detection on a dry plate (0.5 ng) was found to be lower than on a wetted plate (2 ng). Precision was found to be 1-4% RSD intra-plate and 8-14% RSD inter-plate. Results are compared with quantification of the same analyte on dried plates.

Edmund T. Bergström - One of the best experts on this subject based on the ideXlab platform.

  • Quantitative ultraviolet measurements on wetted thin-layer chromatography plates using a Charge-Coupled Device Camera.
    Journal of Chromatography A, 2008
    Co-Authors: Michael Lancaster, David M. Goodall, Edmund T. Bergström, Sean Mccrossen, Peter Myers
    Abstract:

    Abstract This paper presents the first study of the UV imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging of spots of benzophenone during and after development was carried out using a Charge-Coupled Device Camera. Limits of detection were found to be 5 ng on a wetted plate and 3 ng for a dry plate and the relationship between peak area and sample loading was found to be linear in the low nanogram range over an order of magnitude for both wet and dry modes with r2 values > 0.99. It was found that UV measurements on wet glass-backed plates suffer from low sensitivity; however, the use of aluminium-backed plates gave increased sensitivity. The apparent absorption coefficient (ɛapp) of 10 AU m2 g−1 at 254 nm is consistent with reflection of the light from the aluminium surface with a double pass through the sorbent layer, and suggests that use of aluminium-backed plates should enable monitoring of separations by UV absorbance during TLC development.

  • Quantitative measurements on wetted thin layer chromatography plates using a charge coupled Device Camera.
    Journal of chromatography. A, 2005
    Co-Authors: Michael Lancaster, David M. Goodall, Edmund T. Bergström, Sean Mccrossen, Peter Myers
    Abstract:

    This paper presents the first study of imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging and quantification of Sudan II after development with dichloromethane was carried out in both reflectance and transmission modes, using a charge coupled Device (CCD) Camera. The relationship between peak area and sample loading was established at low sample loading, and found to be linear over an order of magnitude for both wet and dry modes with r2-values > 0.99. All data processing was carried out using the Beer-Lambert equation. Curvature at high loadings in the plots of integrated absorbance as a function of sample loading was accounted for using an empirical expression designed for use with the Kubelka-Munk treatment and apparent absorbance of the stationary phase due to scattering. Results are consistent with an effective pathlength significantly longer than the thickness of the sorbent layer. The limit of detection on a dry plate (0.5 ng) was found to be lower than on a wetted plate (2 ng). Precision was found to be 1-4% RSD intra-plate and 8-14% RSD inter-plate. Results are compared with quantification of the same analyte on dried plates.

Sean Mccrossen - One of the best experts on this subject based on the ideXlab platform.

  • Quantitative ultraviolet measurements on wetted thin-layer chromatography plates using a Charge-Coupled Device Camera.
    Journal of Chromatography A, 2008
    Co-Authors: Michael Lancaster, David M. Goodall, Edmund T. Bergström, Sean Mccrossen, Peter Myers
    Abstract:

    Abstract This paper presents the first study of the UV imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging of spots of benzophenone during and after development was carried out using a Charge-Coupled Device Camera. Limits of detection were found to be 5 ng on a wetted plate and 3 ng for a dry plate and the relationship between peak area and sample loading was found to be linear in the low nanogram range over an order of magnitude for both wet and dry modes with r2 values > 0.99. It was found that UV measurements on wet glass-backed plates suffer from low sensitivity; however, the use of aluminium-backed plates gave increased sensitivity. The apparent absorption coefficient (ɛapp) of 10 AU m2 g−1 at 254 nm is consistent with reflection of the light from the aluminium surface with a double pass through the sorbent layer, and suggests that use of aluminium-backed plates should enable monitoring of separations by UV absorbance during TLC development.

  • Quantitative measurements on wetted thin layer chromatography plates using a charge coupled Device Camera.
    Journal of chromatography. A, 2005
    Co-Authors: Michael Lancaster, David M. Goodall, Edmund T. Bergström, Sean Mccrossen, Peter Myers
    Abstract:

    This paper presents the first study of imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging and quantification of Sudan II after development with dichloromethane was carried out in both reflectance and transmission modes, using a charge coupled Device (CCD) Camera. The relationship between peak area and sample loading was established at low sample loading, and found to be linear over an order of magnitude for both wet and dry modes with r2-values > 0.99. All data processing was carried out using the Beer-Lambert equation. Curvature at high loadings in the plots of integrated absorbance as a function of sample loading was accounted for using an empirical expression designed for use with the Kubelka-Munk treatment and apparent absorbance of the stationary phase due to scattering. Results are consistent with an effective pathlength significantly longer than the thickness of the sorbent layer. The limit of detection on a dry plate (0.5 ng) was found to be lower than on a wetted plate (2 ng). Precision was found to be 1-4% RSD intra-plate and 8-14% RSD inter-plate. Results are compared with quantification of the same analyte on dried plates.

David M. Goodall - One of the best experts on this subject based on the ideXlab platform.

  • Quantitative ultraviolet measurements on wetted thin-layer chromatography plates using a Charge-Coupled Device Camera.
    Journal of Chromatography A, 2008
    Co-Authors: Michael Lancaster, David M. Goodall, Edmund T. Bergström, Sean Mccrossen, Peter Myers
    Abstract:

    Abstract This paper presents the first study of the UV imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging of spots of benzophenone during and after development was carried out using a Charge-Coupled Device Camera. Limits of detection were found to be 5 ng on a wetted plate and 3 ng for a dry plate and the relationship between peak area and sample loading was found to be linear in the low nanogram range over an order of magnitude for both wet and dry modes with r2 values > 0.99. It was found that UV measurements on wet glass-backed plates suffer from low sensitivity; however, the use of aluminium-backed plates gave increased sensitivity. The apparent absorption coefficient (ɛapp) of 10 AU m2 g−1 at 254 nm is consistent with reflection of the light from the aluminium surface with a double pass through the sorbent layer, and suggests that use of aluminium-backed plates should enable monitoring of separations by UV absorbance during TLC development.

  • Quantitative measurements on wetted thin layer chromatography plates using a charge coupled Device Camera.
    Journal of chromatography. A, 2005
    Co-Authors: Michael Lancaster, David M. Goodall, Edmund T. Bergström, Sean Mccrossen, Peter Myers
    Abstract:

    This paper presents the first study of imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging and quantification of Sudan II after development with dichloromethane was carried out in both reflectance and transmission modes, using a charge coupled Device (CCD) Camera. The relationship between peak area and sample loading was established at low sample loading, and found to be linear over an order of magnitude for both wet and dry modes with r2-values > 0.99. All data processing was carried out using the Beer-Lambert equation. Curvature at high loadings in the plots of integrated absorbance as a function of sample loading was accounted for using an empirical expression designed for use with the Kubelka-Munk treatment and apparent absorbance of the stationary phase due to scattering. Results are consistent with an effective pathlength significantly longer than the thickness of the sorbent layer. The limit of detection on a dry plate (0.5 ng) was found to be lower than on a wetted plate (2 ng). Precision was found to be 1-4% RSD intra-plate and 8-14% RSD inter-plate. Results are compared with quantification of the same analyte on dried plates.