The Experts below are selected from a list of 198 Experts worldwide ranked by ideXlab platform

Sara Ferrando - One of the best experts on this subject based on the ideXlab platform.

  • Vacchi's palatal organ: a widespread trait in Holocephali.
    Journal of fish biology, 2018
    Co-Authors: Brittany Finucci, Lorenzo Gallus, Andrea Amaroli, Simona Candiani, Marino Rottigni, M. A. Masini, Sara Ferrando
    Abstract:

    A palatal organ, possibly used for food sorting and processing, has previously been identified among the vomerine toothplates of the chimaeroid Chimaera Monstrosa. In this study, the palatal organ was described in six additional species, confirming it is a widespread trait among holocephalans. It is proposed that this palatal structure, which appears to differ in shape according to each chimaeroid's degree of durophagy and is not homologous to the palatal structure described in teleosts, be hereby referred to as Vacchi's organ.

  • First detection of taste buds in a chimaeroid fish (Chondrichthyes: Holocephali) and their Gαi-like immunoreactivity
    Neuroscience letters, 2012
    Co-Authors: Sara Ferrando, Lorenzo Gallus, Chiara Gambardella, Maria Angela Masini, Alessia Cutolo, Marino Vacchi
    Abstract:

    The mucosa covering the tongue of the Chimaera Monstrosa has been investigated with histological and immunohistochemical methods allowing to describe, for the first time, gustatory structures (taste buds) in this subclass of cartilaginous fish. G-protein-alpha-subunit-inhibitory-like (Gαi-like) immunoreactivity has been detected in the taste buds of C. Monstrosa, as described in other vertebrates. In order to gain confidence on the antiserum used, able to recognize three Gαi proteins in mammals, alignments of the antigenic sequence in mammals and other vertebrates were performed. The data were used for a research of putative genes in the genome of the holocephalan Callorhinchus milii, to date the only cartilaginous fish with a sequenced genome; the highlighted sequences could suggest the presence of all three genes (gnai1, gnai2 and gnai3) in holocephalans. The sequences of the predicted proteins present a high identity with the mammalian proteins.

  • G protein alpha subunits in the olfactory epithelium of the holocephalan fish Chimaera Monstrosa
    Neuroscience letters, 2010
    Co-Authors: Sara Ferrando, Lorenzo Gallus, Chiara Gambardella, Marino Vacchi, Grazia Tagliafierro
    Abstract:

    Receptor neurons in the olfactory and vomeronasal epithelia of vertebrates have dendritic specialization that is correlated to the receptor gene family they express and the G protein coupled with that receptor (in particular the G protein alpha subunit). There are not very many data in the literature about the morphological and molecular features of the olfactory epithelium of Chondrichthyes. In this work, the presence and distribution of different types of G protein alpha subunits (Galpha(o), Galpha(q) and Galpha(olf)) were investigated in the olfactory epithelium of the holocephalan Chimaera Monstrosa using immunohistochemistry. Only Galpha(o)-like immunoreactivity was detected in the microvillous receptor neurons and in numerous axon bundles of the fila olfactoria. These preliminary data are in agreement with studies showing G protein alpha subunits in elasmobranchs and support the data present in the literature about putative odorant receptor families in the sequenced genome of the holocephalan Callorhinchus milii.

Marino Vacchi - One of the best experts on this subject based on the ideXlab platform.

  • First detection of taste buds in a chimaeroid fish (Chondrichthyes: Holocephali) and their Gαi-like immunoreactivity
    Neuroscience letters, 2012
    Co-Authors: Sara Ferrando, Lorenzo Gallus, Chiara Gambardella, Maria Angela Masini, Alessia Cutolo, Marino Vacchi
    Abstract:

    The mucosa covering the tongue of the Chimaera Monstrosa has been investigated with histological and immunohistochemical methods allowing to describe, for the first time, gustatory structures (taste buds) in this subclass of cartilaginous fish. G-protein-alpha-subunit-inhibitory-like (Gαi-like) immunoreactivity has been detected in the taste buds of C. Monstrosa, as described in other vertebrates. In order to gain confidence on the antiserum used, able to recognize three Gαi proteins in mammals, alignments of the antigenic sequence in mammals and other vertebrates were performed. The data were used for a research of putative genes in the genome of the holocephalan Callorhinchus milii, to date the only cartilaginous fish with a sequenced genome; the highlighted sequences could suggest the presence of all three genes (gnai1, gnai2 and gnai3) in holocephalans. The sequences of the predicted proteins present a high identity with the mammalian proteins.

  • G protein alpha subunits in the olfactory epithelium of the holocephalan fish Chimaera Monstrosa
    Neuroscience letters, 2010
    Co-Authors: Sara Ferrando, Lorenzo Gallus, Chiara Gambardella, Marino Vacchi, Grazia Tagliafierro
    Abstract:

    Receptor neurons in the olfactory and vomeronasal epithelia of vertebrates have dendritic specialization that is correlated to the receptor gene family they express and the G protein coupled with that receptor (in particular the G protein alpha subunit). There are not very many data in the literature about the morphological and molecular features of the olfactory epithelium of Chondrichthyes. In this work, the presence and distribution of different types of G protein alpha subunits (Galpha(o), Galpha(q) and Galpha(olf)) were investigated in the olfactory epithelium of the holocephalan Chimaera Monstrosa using immunohistochemistry. Only Galpha(o)-like immunoreactivity was detected in the microvillous receptor neurons and in numerous axon bundles of the fila olfactoria. These preliminary data are in agreement with studies showing G protein alpha subunits in elasmobranchs and support the data present in the literature about putative odorant receptor families in the sequenced genome of the holocephalan Callorhinchus milii.

  • GnRH immunodetection in the brain of the holocephalan fish Chimaera Monstrosa L.: correlation to oocyte maturation.
    General and comparative endocrinology, 2008
    Co-Authors: Maria Angela Masini, Marino Vacchi, Paola Prato, Bianca Maria Uva
    Abstract:

    Abstract Chimera Monstrosa (rabbit fish) like other holocephalans is a rare, delicate deep sea fish. Owing to the difficulty of sampling individuals in good shape, there is a paucity of information available on the morphology and physiology of this species especially concerning reproduction. In holocephalans, a hypothalamus–pituitary–gonadal axis has been postulated and a GnRH molecule identical to cGnRH II has been identified. The aim of the present study was to correlate the presence of steroidogenic enzymes in the ovarian follicles with the presence of GnRH in the hypothalamus. Estrogens, the steroids that trigger the accumulation of yolk (vitellogenesis) in the oocytes are synthesized by the somatic cells of the follicle in the vitellogenic stages via a cascade of steroid dehydrogenases involving 3β-hydroxysteroid-dehydrogenase (3β-HSD; in the inner thecal layer) and aromatase cytochrome (P450; granulosa layer). Our results showed that 3β-HSD is present concomitant with the presence of cGnRH II in the preoptic area and in the ventral hypothalamus. Another form of immunoreactive GnRH, mGnRH is also present in the brain of C. Monstrosa. It is localized in the ventral telencephalon and in the midbrain caudal diencephalon (boundary between ventral thalamus and tegmentum of the mesencephalon). This form of GnRH is probably correlated with sexual behaviour.

Lorenzo Gallus - One of the best experts on this subject based on the ideXlab platform.

  • Vacchi's palatal organ: a widespread trait in Holocephali.
    Journal of fish biology, 2018
    Co-Authors: Brittany Finucci, Lorenzo Gallus, Andrea Amaroli, Simona Candiani, Marino Rottigni, M. A. Masini, Sara Ferrando
    Abstract:

    A palatal organ, possibly used for food sorting and processing, has previously been identified among the vomerine toothplates of the chimaeroid Chimaera Monstrosa. In this study, the palatal organ was described in six additional species, confirming it is a widespread trait among holocephalans. It is proposed that this palatal structure, which appears to differ in shape according to each chimaeroid's degree of durophagy and is not homologous to the palatal structure described in teleosts, be hereby referred to as Vacchi's organ.

  • First detection of taste buds in a chimaeroid fish (Chondrichthyes: Holocephali) and their Gαi-like immunoreactivity
    Neuroscience letters, 2012
    Co-Authors: Sara Ferrando, Lorenzo Gallus, Chiara Gambardella, Maria Angela Masini, Alessia Cutolo, Marino Vacchi
    Abstract:

    The mucosa covering the tongue of the Chimaera Monstrosa has been investigated with histological and immunohistochemical methods allowing to describe, for the first time, gustatory structures (taste buds) in this subclass of cartilaginous fish. G-protein-alpha-subunit-inhibitory-like (Gαi-like) immunoreactivity has been detected in the taste buds of C. Monstrosa, as described in other vertebrates. In order to gain confidence on the antiserum used, able to recognize three Gαi proteins in mammals, alignments of the antigenic sequence in mammals and other vertebrates were performed. The data were used for a research of putative genes in the genome of the holocephalan Callorhinchus milii, to date the only cartilaginous fish with a sequenced genome; the highlighted sequences could suggest the presence of all three genes (gnai1, gnai2 and gnai3) in holocephalans. The sequences of the predicted proteins present a high identity with the mammalian proteins.

  • G protein alpha subunits in the olfactory epithelium of the holocephalan fish Chimaera Monstrosa
    Neuroscience letters, 2010
    Co-Authors: Sara Ferrando, Lorenzo Gallus, Chiara Gambardella, Marino Vacchi, Grazia Tagliafierro
    Abstract:

    Receptor neurons in the olfactory and vomeronasal epithelia of vertebrates have dendritic specialization that is correlated to the receptor gene family they express and the G protein coupled with that receptor (in particular the G protein alpha subunit). There are not very many data in the literature about the morphological and molecular features of the olfactory epithelium of Chondrichthyes. In this work, the presence and distribution of different types of G protein alpha subunits (Galpha(o), Galpha(q) and Galpha(olf)) were investigated in the olfactory epithelium of the holocephalan Chimaera Monstrosa using immunohistochemistry. Only Galpha(o)-like immunoreactivity was detected in the microvillous receptor neurons and in numerous axon bundles of the fila olfactoria. These preliminary data are in agreement with studies showing G protein alpha subunits in elasmobranchs and support the data present in the literature about putative odorant receptor families in the sequenced genome of the holocephalan Callorhinchus milii.

Maria Angela Masini - One of the best experts on this subject based on the ideXlab platform.

  • First detection of taste buds in a chimaeroid fish (Chondrichthyes: Holocephali) and their Gαi-like immunoreactivity
    Neuroscience letters, 2012
    Co-Authors: Sara Ferrando, Lorenzo Gallus, Chiara Gambardella, Maria Angela Masini, Alessia Cutolo, Marino Vacchi
    Abstract:

    The mucosa covering the tongue of the Chimaera Monstrosa has been investigated with histological and immunohistochemical methods allowing to describe, for the first time, gustatory structures (taste buds) in this subclass of cartilaginous fish. G-protein-alpha-subunit-inhibitory-like (Gαi-like) immunoreactivity has been detected in the taste buds of C. Monstrosa, as described in other vertebrates. In order to gain confidence on the antiserum used, able to recognize three Gαi proteins in mammals, alignments of the antigenic sequence in mammals and other vertebrates were performed. The data were used for a research of putative genes in the genome of the holocephalan Callorhinchus milii, to date the only cartilaginous fish with a sequenced genome; the highlighted sequences could suggest the presence of all three genes (gnai1, gnai2 and gnai3) in holocephalans. The sequences of the predicted proteins present a high identity with the mammalian proteins.

  • GnRH immunodetection in the brain of the holocephalan fish Chimaera Monstrosa L.: correlation to oocyte maturation.
    General and comparative endocrinology, 2008
    Co-Authors: Maria Angela Masini, Marino Vacchi, Paola Prato, Bianca Maria Uva
    Abstract:

    Abstract Chimera Monstrosa (rabbit fish) like other holocephalans is a rare, delicate deep sea fish. Owing to the difficulty of sampling individuals in good shape, there is a paucity of information available on the morphology and physiology of this species especially concerning reproduction. In holocephalans, a hypothalamus–pituitary–gonadal axis has been postulated and a GnRH molecule identical to cGnRH II has been identified. The aim of the present study was to correlate the presence of steroidogenic enzymes in the ovarian follicles with the presence of GnRH in the hypothalamus. Estrogens, the steroids that trigger the accumulation of yolk (vitellogenesis) in the oocytes are synthesized by the somatic cells of the follicle in the vitellogenic stages via a cascade of steroid dehydrogenases involving 3β-hydroxysteroid-dehydrogenase (3β-HSD; in the inner thecal layer) and aromatase cytochrome (P450; granulosa layer). Our results showed that 3β-HSD is present concomitant with the presence of cGnRH II in the preoptic area and in the ventral hypothalamus. Another form of immunoreactive GnRH, mGnRH is also present in the brain of C. Monstrosa. It is localized in the ventral telencephalon and in the midbrain caudal diencephalon (boundary between ventral thalamus and tegmentum of the mesencephalon). This form of GnRH is probably correlated with sexual behaviour.

Chiara Gambardella - One of the best experts on this subject based on the ideXlab platform.

  • First detection of taste buds in a chimaeroid fish (Chondrichthyes: Holocephali) and their Gαi-like immunoreactivity
    Neuroscience letters, 2012
    Co-Authors: Sara Ferrando, Lorenzo Gallus, Chiara Gambardella, Maria Angela Masini, Alessia Cutolo, Marino Vacchi
    Abstract:

    The mucosa covering the tongue of the Chimaera Monstrosa has been investigated with histological and immunohistochemical methods allowing to describe, for the first time, gustatory structures (taste buds) in this subclass of cartilaginous fish. G-protein-alpha-subunit-inhibitory-like (Gαi-like) immunoreactivity has been detected in the taste buds of C. Monstrosa, as described in other vertebrates. In order to gain confidence on the antiserum used, able to recognize three Gαi proteins in mammals, alignments of the antigenic sequence in mammals and other vertebrates were performed. The data were used for a research of putative genes in the genome of the holocephalan Callorhinchus milii, to date the only cartilaginous fish with a sequenced genome; the highlighted sequences could suggest the presence of all three genes (gnai1, gnai2 and gnai3) in holocephalans. The sequences of the predicted proteins present a high identity with the mammalian proteins.

  • G protein alpha subunits in the olfactory epithelium of the holocephalan fish Chimaera Monstrosa
    Neuroscience letters, 2010
    Co-Authors: Sara Ferrando, Lorenzo Gallus, Chiara Gambardella, Marino Vacchi, Grazia Tagliafierro
    Abstract:

    Receptor neurons in the olfactory and vomeronasal epithelia of vertebrates have dendritic specialization that is correlated to the receptor gene family they express and the G protein coupled with that receptor (in particular the G protein alpha subunit). There are not very many data in the literature about the morphological and molecular features of the olfactory epithelium of Chondrichthyes. In this work, the presence and distribution of different types of G protein alpha subunits (Galpha(o), Galpha(q) and Galpha(olf)) were investigated in the olfactory epithelium of the holocephalan Chimaera Monstrosa using immunohistochemistry. Only Galpha(o)-like immunoreactivity was detected in the microvillous receptor neurons and in numerous axon bundles of the fila olfactoria. These preliminary data are in agreement with studies showing G protein alpha subunits in elasmobranchs and support the data present in the literature about putative odorant receptor families in the sequenced genome of the holocephalan Callorhinchus milii.