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Michal Sienkiewicz - One of the best experts on this subject based on the ideXlab platform.

Vadim A Soloshonok - One of the best experts on this subject based on the ideXlab platform.

Aneta Nodzewska - One of the best experts on this subject based on the ideXlab platform.

Li Hai-lan - One of the best experts on this subject based on the ideXlab platform.

  • Resolution of the isoprenalini enantiomers derivatized with fluorescent Chiral Reagents by reversed-phase high-performance liquid chromatography
    The Chinese Journal of Modern Applied Pharmacy, 2005
    Co-Authors: Li Hai-lan
    Abstract:

    OBJECTIVE This paper describes a new method developed to separate isoprenalini enantiomers derivatized with fluorescent Chiral Reagent, DBD-PyNCS:R(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-iso-thiocyanatopyrrolidino)-2,1,3-benzoxadiazole, by reversed-phase high-performance liquid chromatography, on a Diamonsil~(TM)C_(18) column(150 mm×4.6 mm, i.d.,5μm),with a mobile phase of water-acetonitrile(65:35, v/v) and with a fluorescent detector : the excitation wavelength was 460nm and the emission wavelength was 550nm. The column was eluted at 40℃. Flow rate was 1mL/min. METHODS Fluorescent Chiral Reagent(36mmol/L,10μL) reacted with isoprenalini enantiomers(1mmol/L, 10μL),and then 10μL 20% pyridine acetonitrile solution was added.The resulting mixture was stirred for 1 min.And reacted at 65℃ for 35 min. and then a 10μL aliquot of the diastereomeric derivatives was injected directly into the chromatography. RESULTST The diastereomeric compounds were efficiently separated. The Rs value was 6.25; a = 1.20; L-isoprenalini k~(/ )=10.23;D-isoprenalini k~(/) =8.56. The retention time of D-isoprenalini and L-isoprenalini was 26.5min and 31.1min respectively. The linear range was 3.23 10~(-4)mol/L~2.63 10~(-3)mol /L (r=0.9997). The RSD. (L-isoprenalini concentration 1mmol/L) was 1.83%(n=7), the detection limits of isoprenalini enantiomers were 2.1 10~(-11 )mol /L(S/N=3).CONCLUSION The diastereomeric compounds were efficiently and rapidlly separated.

Toshimasa Toyooka - One of the best experts on this subject based on the ideXlab platform.

  • towards the Chiral metabolomics liquid chromatography mass spectrometry based dl amino acid analysis after labeling with a new Chiral Reagent s 2 5 dioxopyrrolidin 1 yl 1 4 6 dimethoxy 1 3 5 triazin 2 yl pyrrolidine 2 carboxylate and the application
    Analytica Chimica Acta, 2015
    Co-Authors: Toshiki Mochizuki, Takahiro Takayama, Kenichiro Todoroki, Koichi Inoue, Jun Zhe Min, Toshimasa Toyooka
    Abstract:

    Abstract A novel triazine-type Chiral derivatization Reagent, i.e., (S)-2,5-dioxopyrrolidin-1-yl-1-(4,6-dimethoxy-1,3,5-triazin-2-yl) pyrrolidine-2-carboxylate (DMT-(S)-Pro-OSu), was developed for the highly sensitive and selective detection of Chiral amines and amino acids by UPLC–MS/MS analysis. The enantiomers of amino acids were easily labeled with the Reagents at room temperature within 40 min in an alkaline medium containing triethylamine. The diastereomers derived from proteolytic amino acids, except serine, were well separated under isocratic elution conditions by reversed-phase chromatography using an ODS column (Rs = 1.2–9.0). dl -Serine was separated by use of an ADME column which has relatively higher polar surface than the conventional ODS column. The characteristic product ions, i.e., m/z 195.3 and m/z 209.3, were detected from all the diastereomers by the collision-induced dissociation of the protonated molecule. A highly sensitive detection on the amol–fmol level was obtained from the selected reaction monitoring (SRM) chromatogram. The Chiral amines (e.g., adrenaline and noradrenaline) labeled with DMT-(S)-Pro-OSu were also well separated and sensitively detected by the present procedure. The method using DMT-(S)-Pro-OSu was used for the determination of dl -amino acids in the human saliva from healthy volunteers. Various l -amino acids were identified in the saliva. Furthermore, d -alanine ( d -Ala) and d -proline ( d -Pro) were also detected in relatively high concentrations (>5%). The ratio was higher in male saliva than in female saliva. However, the difference in the ratio of d -Ala for one day was not very high and the effect of foods and beverage seemed to be negligible. Based on the results using l -Ala-d3, the d -Ala in saliva seemed to be produced due to the racemization with some enzymes such as racemase. The racemization reaction was reversible, i.e., d -Ala-d3 was also racemized to l -Ala-d3 in saliva. Thus, care should be taken during the analysis of dl -amino acids in saliva. The present method using DMT-(S)-Pro-OSu may be applicable for the determination of Chiral amine metabolomics, because the resulting derivatives produce the same product ions without relation to the compounds and show highly sensitive detection in the SRM mode of MS/MS. Consequently, DMT-(S)-Pro-OSu seems to be a useful Chiral derivatization Reagent for the determination of amines and amino acids in biological samples.