The Experts below are selected from a list of 1335 Experts worldwide ranked by ideXlab platform
Karine Vallée-réhel - One of the best experts on this subject based on the ideXlab platform.
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Monohalogenated maleimides as potential agents for the inhibition of Pseudomonas aeruginosa biofilm
Biofouling, 2010Co-Authors: David Carteau, Emmanuelle Soum-soutéra, Chrystèle Dufau, Stephane Cerantola, Fabienne Faÿ, Karine Vallée-réhelAbstract:New monohalogenated maleimide Derivatives (with bromine, Chlorine or iodine) were synthesized to test the effect of halogen atoms in inhibiting the formation of Pseudomonas aeruginosa biofilm. The evaluation of their biological activities clearly defines a structure–activity relationship. In this study, the bactericidal action of the three compounds was observed at the concentration range 0.3–5.0 mM on Luria-Bertani agar plates. The halogen atom of these molecules was critical in modulating the antibacterial activity, with a slightly higher effectiveness for Chlorine. Confocal laser scanning microscopy was used to examine P. aeruginosa biofilms cultivated in flow cells. At concentration as low as 40 μM, the bromine and iodine compounds displayed a total inhibition towards the formation of bacterial biofilm. At this concentration, the bacterial attachment to glass surfaces was strongly affected by the presence of bromine and iodine whereas the Chlorine Derivative behaved as a bactericidal compound. A biolu...
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Monohalogenated maleimides as potential agents for the inhibition of Pseudomonas aeruginosa biofilm.
Biofouling, 2010Co-Authors: David Carteau, Emmanuelle Soum-soutéra, Chrystèle Dufau, Stephane Cerantola, Fabienne Faÿ, Karine Vallée-réhelAbstract:New monohalogenated maleimide Derivatives (with bromine, Chlorine or iodine) were synthesized to test the effect of halogen atoms in inhibiting the formation of Pseudomonas aeruginosa biofilm. The evaluation of their biological activities clearly defines a structure-activity relationship. In this study, the bactericidal action of the three compounds was observed at the concentration range 0.3-5.0 mM on Luria-Bertani agar plates. The halogen atom of these molecules was critical in modulating the antibacterial activity, with a slightly higher effectiveness for Chlorine. Confocal laser scanning microscopy was used to examine P. aeruginosa biofilms cultivated in flow cells. At concentration as low as 40 microM, the bromine and iodine compounds displayed a total inhibition towards the formation of bacterial biofilm. At this concentration, the bacterial attachment to glass surfaces was strongly affected by the presence of bromine and iodine whereas the Chlorine Derivative behaved as a bactericidal compound. A bioluminescent reporter strain was then used to detect the effect of the chemically synthesized maleimides on quorum sensing (QS) in P. aeruginosa. At the concentration range 10-100 microM, bioluminescence assays reveal that halogenated maleimides were able to interfere with the QS of the bacterium. Although the relationship between the weak inhibition of cell-to-cell communication (15-55% of the signal) and the high inhibition of biofilm formation has not been elucidated clearly, the results demonstrate that bromo- and iodo-N-substituted maleimides bromine and iodine may be used as new potent inhibitors that control bacterial biofilms.
David Carteau - One of the best experts on this subject based on the ideXlab platform.
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Monohalogenated maleimides as potential agents for the inhibition of Pseudomonas aeruginosa biofilm
Biofouling, 2010Co-Authors: David Carteau, Emmanuelle Soum-soutéra, Chrystèle Dufau, Stephane Cerantola, Fabienne Faÿ, Karine Vallée-réhelAbstract:New monohalogenated maleimide Derivatives (with bromine, Chlorine or iodine) were synthesized to test the effect of halogen atoms in inhibiting the formation of Pseudomonas aeruginosa biofilm. The evaluation of their biological activities clearly defines a structure–activity relationship. In this study, the bactericidal action of the three compounds was observed at the concentration range 0.3–5.0 mM on Luria-Bertani agar plates. The halogen atom of these molecules was critical in modulating the antibacterial activity, with a slightly higher effectiveness for Chlorine. Confocal laser scanning microscopy was used to examine P. aeruginosa biofilms cultivated in flow cells. At concentration as low as 40 μM, the bromine and iodine compounds displayed a total inhibition towards the formation of bacterial biofilm. At this concentration, the bacterial attachment to glass surfaces was strongly affected by the presence of bromine and iodine whereas the Chlorine Derivative behaved as a bactericidal compound. A biolu...
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Monohalogenated maleimides as potential agents for the inhibition of Pseudomonas aeruginosa biofilm.
Biofouling, 2010Co-Authors: David Carteau, Emmanuelle Soum-soutéra, Chrystèle Dufau, Stephane Cerantola, Fabienne Faÿ, Karine Vallée-réhelAbstract:New monohalogenated maleimide Derivatives (with bromine, Chlorine or iodine) were synthesized to test the effect of halogen atoms in inhibiting the formation of Pseudomonas aeruginosa biofilm. The evaluation of their biological activities clearly defines a structure-activity relationship. In this study, the bactericidal action of the three compounds was observed at the concentration range 0.3-5.0 mM on Luria-Bertani agar plates. The halogen atom of these molecules was critical in modulating the antibacterial activity, with a slightly higher effectiveness for Chlorine. Confocal laser scanning microscopy was used to examine P. aeruginosa biofilms cultivated in flow cells. At concentration as low as 40 microM, the bromine and iodine compounds displayed a total inhibition towards the formation of bacterial biofilm. At this concentration, the bacterial attachment to glass surfaces was strongly affected by the presence of bromine and iodine whereas the Chlorine Derivative behaved as a bactericidal compound. A bioluminescent reporter strain was then used to detect the effect of the chemically synthesized maleimides on quorum sensing (QS) in P. aeruginosa. At the concentration range 10-100 microM, bioluminescence assays reveal that halogenated maleimides were able to interfere with the QS of the bacterium. Although the relationship between the weak inhibition of cell-to-cell communication (15-55% of the signal) and the high inhibition of biofilm formation has not been elucidated clearly, the results demonstrate that bromo- and iodo-N-substituted maleimides bromine and iodine may be used as new potent inhibitors that control bacterial biofilms.
Yoshiki Katayama - One of the best experts on this subject based on the ideXlab platform.
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rapid and selective determination of free Chlorine in aqueous solution using electrophilic addition to styrene by gas chromatography mass spectrometry
Talanta, 2013Co-Authors: Kengo Wakigawa, Akinaga Gohda, Sunao Fukushima, Takuro Niidome, Takeshi Mori, Yoshiki KatayamaAbstract:Abstract We developed a rapid and selective method for determination of free Chlorine in aqueous solution by gas chromatography/mass spectrometry for the first time. Free Chlorine was converted to styrene chlorohydrin using electrophilic addition to styrene in sodium acetate buffer solution (pH 5). The Chlorine Derivative obtained was extracted with chloroform, and then analyzed by GC/MS. The calibration curve showed good linearity from 0.2–100 μg/mL (as available Chlorine). The detection limit was 0.1 μg/mL, and the intra- and interday accuracy were measured at concentrations of 10, 50, and 75 μg/mL to be −1.3 to 6.9% (intraday) and 3.8–8.0% (interday) as % Bias. The precision was between 1.4 and 4.5% as % RSD. These results indicate that this method is a superior technique for the identification of free Chlorine. This method was successfully applied to quantification in commercial samples and in samples of a criminal case.
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Rapid and selective determination of free Chlorine in aqueous solution using electrophilic addition to styrene by gas chromatography/mass spectrometry.
Talanta, 2012Co-Authors: Kengo Wakigawa, Akinaga Gohda, Sunao Fukushima, Takuro Niidome, Takeshi Mori, Yoshiki KatayamaAbstract:Abstract We developed a rapid and selective method for determination of free Chlorine in aqueous solution by gas chromatography/mass spectrometry for the first time. Free Chlorine was converted to styrene chlorohydrin using electrophilic addition to styrene in sodium acetate buffer solution (pH 5). The Chlorine Derivative obtained was extracted with chloroform, and then analyzed by GC/MS. The calibration curve showed good linearity from 0.2–100 μg/mL (as available Chlorine). The detection limit was 0.1 μg/mL, and the intra- and interday accuracy were measured at concentrations of 10, 50, and 75 μg/mL to be −1.3 to 6.9% (intraday) and 3.8–8.0% (interday) as % Bias. The precision was between 1.4 and 4.5% as % RSD. These results indicate that this method is a superior technique for the identification of free Chlorine. This method was successfully applied to quantification in commercial samples and in samples of a criminal case.
Chrystèle Dufau - One of the best experts on this subject based on the ideXlab platform.
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Monohalogenated maleimides as potential agents for the inhibition of Pseudomonas aeruginosa biofilm
Biofouling, 2010Co-Authors: David Carteau, Emmanuelle Soum-soutéra, Chrystèle Dufau, Stephane Cerantola, Fabienne Faÿ, Karine Vallée-réhelAbstract:New monohalogenated maleimide Derivatives (with bromine, Chlorine or iodine) were synthesized to test the effect of halogen atoms in inhibiting the formation of Pseudomonas aeruginosa biofilm. The evaluation of their biological activities clearly defines a structure–activity relationship. In this study, the bactericidal action of the three compounds was observed at the concentration range 0.3–5.0 mM on Luria-Bertani agar plates. The halogen atom of these molecules was critical in modulating the antibacterial activity, with a slightly higher effectiveness for Chlorine. Confocal laser scanning microscopy was used to examine P. aeruginosa biofilms cultivated in flow cells. At concentration as low as 40 μM, the bromine and iodine compounds displayed a total inhibition towards the formation of bacterial biofilm. At this concentration, the bacterial attachment to glass surfaces was strongly affected by the presence of bromine and iodine whereas the Chlorine Derivative behaved as a bactericidal compound. A biolu...
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Monohalogenated maleimides as potential agents for the inhibition of Pseudomonas aeruginosa biofilm.
Biofouling, 2010Co-Authors: David Carteau, Emmanuelle Soum-soutéra, Chrystèle Dufau, Stephane Cerantola, Fabienne Faÿ, Karine Vallée-réhelAbstract:New monohalogenated maleimide Derivatives (with bromine, Chlorine or iodine) were synthesized to test the effect of halogen atoms in inhibiting the formation of Pseudomonas aeruginosa biofilm. The evaluation of their biological activities clearly defines a structure-activity relationship. In this study, the bactericidal action of the three compounds was observed at the concentration range 0.3-5.0 mM on Luria-Bertani agar plates. The halogen atom of these molecules was critical in modulating the antibacterial activity, with a slightly higher effectiveness for Chlorine. Confocal laser scanning microscopy was used to examine P. aeruginosa biofilms cultivated in flow cells. At concentration as low as 40 microM, the bromine and iodine compounds displayed a total inhibition towards the formation of bacterial biofilm. At this concentration, the bacterial attachment to glass surfaces was strongly affected by the presence of bromine and iodine whereas the Chlorine Derivative behaved as a bactericidal compound. A bioluminescent reporter strain was then used to detect the effect of the chemically synthesized maleimides on quorum sensing (QS) in P. aeruginosa. At the concentration range 10-100 microM, bioluminescence assays reveal that halogenated maleimides were able to interfere with the QS of the bacterium. Although the relationship between the weak inhibition of cell-to-cell communication (15-55% of the signal) and the high inhibition of biofilm formation has not been elucidated clearly, the results demonstrate that bromo- and iodo-N-substituted maleimides bromine and iodine may be used as new potent inhibitors that control bacterial biofilms.
Emmanuelle Soum-soutéra - One of the best experts on this subject based on the ideXlab platform.
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Monohalogenated maleimides as potential agents for the inhibition of Pseudomonas aeruginosa biofilm
Biofouling, 2010Co-Authors: David Carteau, Emmanuelle Soum-soutéra, Chrystèle Dufau, Stephane Cerantola, Fabienne Faÿ, Karine Vallée-réhelAbstract:New monohalogenated maleimide Derivatives (with bromine, Chlorine or iodine) were synthesized to test the effect of halogen atoms in inhibiting the formation of Pseudomonas aeruginosa biofilm. The evaluation of their biological activities clearly defines a structure–activity relationship. In this study, the bactericidal action of the three compounds was observed at the concentration range 0.3–5.0 mM on Luria-Bertani agar plates. The halogen atom of these molecules was critical in modulating the antibacterial activity, with a slightly higher effectiveness for Chlorine. Confocal laser scanning microscopy was used to examine P. aeruginosa biofilms cultivated in flow cells. At concentration as low as 40 μM, the bromine and iodine compounds displayed a total inhibition towards the formation of bacterial biofilm. At this concentration, the bacterial attachment to glass surfaces was strongly affected by the presence of bromine and iodine whereas the Chlorine Derivative behaved as a bactericidal compound. A biolu...
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Monohalogenated maleimides as potential agents for the inhibition of Pseudomonas aeruginosa biofilm.
Biofouling, 2010Co-Authors: David Carteau, Emmanuelle Soum-soutéra, Chrystèle Dufau, Stephane Cerantola, Fabienne Faÿ, Karine Vallée-réhelAbstract:New monohalogenated maleimide Derivatives (with bromine, Chlorine or iodine) were synthesized to test the effect of halogen atoms in inhibiting the formation of Pseudomonas aeruginosa biofilm. The evaluation of their biological activities clearly defines a structure-activity relationship. In this study, the bactericidal action of the three compounds was observed at the concentration range 0.3-5.0 mM on Luria-Bertani agar plates. The halogen atom of these molecules was critical in modulating the antibacterial activity, with a slightly higher effectiveness for Chlorine. Confocal laser scanning microscopy was used to examine P. aeruginosa biofilms cultivated in flow cells. At concentration as low as 40 microM, the bromine and iodine compounds displayed a total inhibition towards the formation of bacterial biofilm. At this concentration, the bacterial attachment to glass surfaces was strongly affected by the presence of bromine and iodine whereas the Chlorine Derivative behaved as a bactericidal compound. A bioluminescent reporter strain was then used to detect the effect of the chemically synthesized maleimides on quorum sensing (QS) in P. aeruginosa. At the concentration range 10-100 microM, bioluminescence assays reveal that halogenated maleimides were able to interfere with the QS of the bacterium. Although the relationship between the weak inhibition of cell-to-cell communication (15-55% of the signal) and the high inhibition of biofilm formation has not been elucidated clearly, the results demonstrate that bromo- and iodo-N-substituted maleimides bromine and iodine may be used as new potent inhibitors that control bacterial biofilms.