The Experts below are selected from a list of 12 Experts worldwide ranked by ideXlab platform
J A Syce - One of the best experts on this subject based on the ideXlab platform.
-
the effect of fenfluramine on the pulmonary disposition of 5 hydroxytryptamine in the isolated perfused rat lung a comparison with Chlorphentermine
Journal of Pharmacy and Pharmacology, 2000Co-Authors: P Valodia, J A SyceAbstract:A possible mechanism for fenfluramine-induced pulmonary hypertension has been investigated. Fenfluramine, like Chlorphentermine, may inhibit the pulmonary uptake and/or metabolism of 5-hydroxytryptamine (5-HT). This allows more 5-HT to remain in the pulmonary circulation, where it may exert a greater vasoconstrictor action resulting in pulmonary hypertension. Chlorphentermine has been shown to inhibit the uptake and metabolism of 5-HT. The effect of fenfluramine on the pulmonary disposition of [14C]5-HT has been investigated, in comparison with Chlorphentermine, using a recirculating isolated perfused rat lung system. The pulmonary disposition of [14C]5-HT was assessed by measuring the change in [14C]5-HT concentration in the perfusion medium during the experiment and at the end, and the concentration in the lung at the end of the experiment. The concentration of 5-hydroxyindoleacetic acid, a metabolite of 5-HT, was measured in perfusate and lung samples. Mean pulmonary clearance of 5-HT for the control lung and lungs challenged with either fenfluramine (2.5 μM) or Chlorphentermine (2.5 μM) was 4.514, 1.316 and 1.007 mL min−1, respectively (n = 5). The concentration of 5-HT found in the lungs at the end of the experiment for the control and the lungs preloaded with fenfluramine or Chlorphentermine was 695.05 ± 9.69, 638.65 ± 10.27 and 617.3 ± 14.38 ng g−1, respectively. Fenfluramine, like Chlorphentermine, inhibited the pulmonary disposition of 5-HT resulting in an elevated perfusate level of 5-HT. This is a possible contributing mechanism for fenfluramine-induced pulmonary hypertension. The effect of fenfluramine was less pronounced than Chlorphentermine.
P Valodia - One of the best experts on this subject based on the ideXlab platform.
-
the effect of fenfluramine on the pulmonary disposition of 5 hydroxytryptamine in the isolated perfused rat lung a comparison with Chlorphentermine
Journal of Pharmacy and Pharmacology, 2000Co-Authors: P Valodia, J A SyceAbstract:A possible mechanism for fenfluramine-induced pulmonary hypertension has been investigated. Fenfluramine, like Chlorphentermine, may inhibit the pulmonary uptake and/or metabolism of 5-hydroxytryptamine (5-HT). This allows more 5-HT to remain in the pulmonary circulation, where it may exert a greater vasoconstrictor action resulting in pulmonary hypertension. Chlorphentermine has been shown to inhibit the uptake and metabolism of 5-HT. The effect of fenfluramine on the pulmonary disposition of [14C]5-HT has been investigated, in comparison with Chlorphentermine, using a recirculating isolated perfused rat lung system. The pulmonary disposition of [14C]5-HT was assessed by measuring the change in [14C]5-HT concentration in the perfusion medium during the experiment and at the end, and the concentration in the lung at the end of the experiment. The concentration of 5-hydroxyindoleacetic acid, a metabolite of 5-HT, was measured in perfusate and lung samples. Mean pulmonary clearance of 5-HT for the control lung and lungs challenged with either fenfluramine (2.5 μM) or Chlorphentermine (2.5 μM) was 4.514, 1.316 and 1.007 mL min−1, respectively (n = 5). The concentration of 5-HT found in the lungs at the end of the experiment for the control and the lungs preloaded with fenfluramine or Chlorphentermine was 695.05 ± 9.69, 638.65 ± 10.27 and 617.3 ± 14.38 ng g−1, respectively. Fenfluramine, like Chlorphentermine, inhibited the pulmonary disposition of 5-HT resulting in an elevated perfusate level of 5-HT. This is a possible contributing mechanism for fenfluramine-induced pulmonary hypertension. The effect of fenfluramine was less pronounced than Chlorphentermine.
T. Bredehorn - One of the best experts on this subject based on the ideXlab platform.
-
Chlorphentermine-induced lipidosis in the rat retina: a functional and morphological study
Graefe's Archive for Clinical and Experimental Ophthalmology, 1994Co-Authors: G. Duncker, T. BredehornAbstract:Chronic administration of the cationic amphiphilic anorexigenic drug Chlorphentermine to rats has previously been shown to induce extraocular and ocular lipidosis: large numbers of lipidosis —related cytoplasmic inclusions can be found in the pigment epithelium and smaller numbers in the neuroretina. In the present study, female albino Wistar rats were treated orally with Chlorphentermine (30–45 mg/kg body weight) for 4–16 weeks. The animals were submitted to electroretinography, and the retinae were prepared for histological investigations. Our histological findings corresponded to previous reports. The changes in electroretinographic parameters were low. The clearest change was a reduction of the b-wave amplitude of 20% after 12 and 16 weeks of treatment compared with the values before drug treatment. The a-wave amplitude did not differ from that in the control group. Lipidosis in the neuroretina may be the reason for functional influences on the b-wave amplitude. The function of the receptor cells, which is represented by the a-wave, appeared unaffected by Chlorphentermine.
David R. Elmaleh - One of the best experts on this subject based on the ideXlab platform.
-
selective monomethylation of the primary amine function using 11c ch3i and the n trifluoroacetyl derivative preparation of n 11c methyl Chlorphentermine
Nuclear Medicine and Biology, 1993Co-Authors: H. Kizuka, David R. ElmalehAbstract:Abstract A simple, rapid and efficient method for the preparation of a potential brain blood-flow agent, N -[ 11 C-methyl]-Chlorphentermine ([ 11 C]NMCP), is described. Optimization of the radiochemical yield of [ 11 C]NMCP was accomplished by a Gabriel-like reaction which permits the transformation of a primary amine to a secondary amine through a sequence of acylation, deprotonation, monomethylation and saponification. This method precludes the formation of polymethylated by-products which can reduce radiochemical yields, particularly with low specific activity 11 CO 2 .
G. Duncker - One of the best experts on this subject based on the ideXlab platform.
-
Chlorphentermine-induced lipidosis in the rat retina: a functional and morphological study
Graefe's Archive for Clinical and Experimental Ophthalmology, 1994Co-Authors: G. Duncker, T. BredehornAbstract:Chronic administration of the cationic amphiphilic anorexigenic drug Chlorphentermine to rats has previously been shown to induce extraocular and ocular lipidosis: large numbers of lipidosis —related cytoplasmic inclusions can be found in the pigment epithelium and smaller numbers in the neuroretina. In the present study, female albino Wistar rats were treated orally with Chlorphentermine (30–45 mg/kg body weight) for 4–16 weeks. The animals were submitted to electroretinography, and the retinae were prepared for histological investigations. Our histological findings corresponded to previous reports. The changes in electroretinographic parameters were low. The clearest change was a reduction of the b-wave amplitude of 20% after 12 and 16 weeks of treatment compared with the values before drug treatment. The a-wave amplitude did not differ from that in the control group. Lipidosis in the neuroretina may be the reason for functional influences on the b-wave amplitude. The function of the receptor cells, which is represented by the a-wave, appeared unaffected by Chlorphentermine.