The Experts below are selected from a list of 48 Experts worldwide ranked by ideXlab platform
Donald J Dudley - One of the best experts on this subject based on the ideXlab platform.
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regulation of decidual Cell and Chorion Cell production of interleukin 10 by purified bacterial products
American Journal of Reproductive Immunology, 1997Co-Authors: Donald J Dudley, Samuel S Edwin, Albina Dangerfield, Kirsten Jackson, Michael S TrautmanAbstract:PROBLEM: To determine whether cultured human decidual Cells and Chorion Cells produce interleukin-10 (IL-10) after incubation with purified bacterial products. METHOD OF STUDY: Decidual Cell cultures and Chorion Cell cultures were established by standard techniques. With confluence, monolayers of each culture were incubated with purified bacterial products, including various concentrations of lipopolysaccharide (LPS), lipid A, and lipoteichoic acid (LTA) for 16 hr in quadruplicate. Culture supernatants were collected and assayed for immunodetectable IL-10 by enzyme-linked immunoadsorbent assay (ELISA). RESULTS: Both decidual Cell cultures and Chorion Cell cultures produced significant quantities of IL-10 after stimulation with LPS, lipid A, and LTA. Cultures of decidual Cells produced more IL-10 than did Chorion Cell cultures. CONCLUSIONS: Our data indicate that both maternal decidual Cells and fetally derived Chorion Cells can produce IL-10 after incubation with bacterial virulence factors. This finding contrasts with our previous findings in which Chorion Cells did not produce IL-10 after stimulation with IL-1β, suggesting that Chorion Cell production after incubation with bacterial products is independent of IL-1β. We speculate that the contribution of anti-inflammatory IL-10 production by human gestational tissues to the inflammatory process in these tissues may be overcome or abrogated by the pro-inflammatory process.
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regulation of cultured human Chorion Cell chemokine production by group b streptococci and purified bacterial products
American Journal of Reproductive Immunology, 1996Co-Authors: Donald J Dudley, Samuel S Edwin, Albina Dangerfield, Jennifer Van Waggoner, Murray D MitchellAbstract:PROBLEM : To determine if different strains of group B streptococci (GBS) and purified bacterial products regulate chemokine production by cultured human Chorion Cells. METHOD OF STUDY : Primary cultures of human Chorion Cells were established from placentae isolated from normal women at term gestation having repeat cesarean section. Five different strains of heat-killed GBS were incubated with confluent Chorion Cells at 10 7 bacteria/ml for 16 hours at 37°C. In separate experiments, lipoteichoic acid and sialic acid at various concentrations were incubated with Chorion Cells for 16 hours at 37°C. Culture supernatants were collected and then assayed to determine concentrations of interleukin-8 (IL-8) and macrophage inflammatory protein-1α (MIP-1α) by ELISA. RESULTS : We found that GBS stimulated Chorion Cell production of MIP-1α in a strain-specific fashion. We also found that both lipoteichoic acid and sialic acid stimulated concentration-dependent increases in Chorion Cell IL-8 production. Chorion Cells, however, did not increase MIP-1α production in response to either lipoteichoic acid or sialic acid. Two strains of GBS tested induced concentration-dependent increases in both IL-8 and MIP-1α, but both stimulated IL-8 production to a greater extent. Similarly, IL-1β also caused Chorion Cells to produce more IL-8 than MIP-1α. CONCLUSIONS : Our data are the first to show that GBS and purified bacterial products can stimulate chemokine production by fetal gestational tissues. We suggest that Chorion Cells may produce specific types of chemokines to attract different types of inflammatory Cells and thus may participate in the pathophysiology of infection-mediated preterm labor by directing specific inflammatory responses.
Albina Dangerfield - One of the best experts on this subject based on the ideXlab platform.
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regulation of decidual Cell and Chorion Cell production of interleukin 10 by purified bacterial products
American Journal of Reproductive Immunology, 1997Co-Authors: Donald J Dudley, Samuel S Edwin, Albina Dangerfield, Kirsten Jackson, Michael S TrautmanAbstract:PROBLEM: To determine whether cultured human decidual Cells and Chorion Cells produce interleukin-10 (IL-10) after incubation with purified bacterial products. METHOD OF STUDY: Decidual Cell cultures and Chorion Cell cultures were established by standard techniques. With confluence, monolayers of each culture were incubated with purified bacterial products, including various concentrations of lipopolysaccharide (LPS), lipid A, and lipoteichoic acid (LTA) for 16 hr in quadruplicate. Culture supernatants were collected and assayed for immunodetectable IL-10 by enzyme-linked immunoadsorbent assay (ELISA). RESULTS: Both decidual Cell cultures and Chorion Cell cultures produced significant quantities of IL-10 after stimulation with LPS, lipid A, and LTA. Cultures of decidual Cells produced more IL-10 than did Chorion Cell cultures. CONCLUSIONS: Our data indicate that both maternal decidual Cells and fetally derived Chorion Cells can produce IL-10 after incubation with bacterial virulence factors. This finding contrasts with our previous findings in which Chorion Cells did not produce IL-10 after stimulation with IL-1β, suggesting that Chorion Cell production after incubation with bacterial products is independent of IL-1β. We speculate that the contribution of anti-inflammatory IL-10 production by human gestational tissues to the inflammatory process in these tissues may be overcome or abrogated by the pro-inflammatory process.
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regulation of cultured human Chorion Cell chemokine production by group b streptococci and purified bacterial products
American Journal of Reproductive Immunology, 1996Co-Authors: Donald J Dudley, Samuel S Edwin, Albina Dangerfield, Jennifer Van Waggoner, Murray D MitchellAbstract:PROBLEM : To determine if different strains of group B streptococci (GBS) and purified bacterial products regulate chemokine production by cultured human Chorion Cells. METHOD OF STUDY : Primary cultures of human Chorion Cells were established from placentae isolated from normal women at term gestation having repeat cesarean section. Five different strains of heat-killed GBS were incubated with confluent Chorion Cells at 10 7 bacteria/ml for 16 hours at 37°C. In separate experiments, lipoteichoic acid and sialic acid at various concentrations were incubated with Chorion Cells for 16 hours at 37°C. Culture supernatants were collected and then assayed to determine concentrations of interleukin-8 (IL-8) and macrophage inflammatory protein-1α (MIP-1α) by ELISA. RESULTS : We found that GBS stimulated Chorion Cell production of MIP-1α in a strain-specific fashion. We also found that both lipoteichoic acid and sialic acid stimulated concentration-dependent increases in Chorion Cell IL-8 production. Chorion Cells, however, did not increase MIP-1α production in response to either lipoteichoic acid or sialic acid. Two strains of GBS tested induced concentration-dependent increases in both IL-8 and MIP-1α, but both stimulated IL-8 production to a greater extent. Similarly, IL-1β also caused Chorion Cells to produce more IL-8 than MIP-1α. CONCLUSIONS : Our data are the first to show that GBS and purified bacterial products can stimulate chemokine production by fetal gestational tissues. We suggest that Chorion Cells may produce specific types of chemokines to attract different types of inflammatory Cells and thus may participate in the pathophysiology of infection-mediated preterm labor by directing specific inflammatory responses.
Samuel S Edwin - One of the best experts on this subject based on the ideXlab platform.
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regulation of decidual Cell and Chorion Cell production of interleukin 10 by purified bacterial products
American Journal of Reproductive Immunology, 1997Co-Authors: Donald J Dudley, Samuel S Edwin, Albina Dangerfield, Kirsten Jackson, Michael S TrautmanAbstract:PROBLEM: To determine whether cultured human decidual Cells and Chorion Cells produce interleukin-10 (IL-10) after incubation with purified bacterial products. METHOD OF STUDY: Decidual Cell cultures and Chorion Cell cultures were established by standard techniques. With confluence, monolayers of each culture were incubated with purified bacterial products, including various concentrations of lipopolysaccharide (LPS), lipid A, and lipoteichoic acid (LTA) for 16 hr in quadruplicate. Culture supernatants were collected and assayed for immunodetectable IL-10 by enzyme-linked immunoadsorbent assay (ELISA). RESULTS: Both decidual Cell cultures and Chorion Cell cultures produced significant quantities of IL-10 after stimulation with LPS, lipid A, and LTA. Cultures of decidual Cells produced more IL-10 than did Chorion Cell cultures. CONCLUSIONS: Our data indicate that both maternal decidual Cells and fetally derived Chorion Cells can produce IL-10 after incubation with bacterial virulence factors. This finding contrasts with our previous findings in which Chorion Cells did not produce IL-10 after stimulation with IL-1β, suggesting that Chorion Cell production after incubation with bacterial products is independent of IL-1β. We speculate that the contribution of anti-inflammatory IL-10 production by human gestational tissues to the inflammatory process in these tissues may be overcome or abrogated by the pro-inflammatory process.
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regulation of cultured human Chorion Cell chemokine production by group b streptococci and purified bacterial products
American Journal of Reproductive Immunology, 1996Co-Authors: Donald J Dudley, Samuel S Edwin, Albina Dangerfield, Jennifer Van Waggoner, Murray D MitchellAbstract:PROBLEM : To determine if different strains of group B streptococci (GBS) and purified bacterial products regulate chemokine production by cultured human Chorion Cells. METHOD OF STUDY : Primary cultures of human Chorion Cells were established from placentae isolated from normal women at term gestation having repeat cesarean section. Five different strains of heat-killed GBS were incubated with confluent Chorion Cells at 10 7 bacteria/ml for 16 hours at 37°C. In separate experiments, lipoteichoic acid and sialic acid at various concentrations were incubated with Chorion Cells for 16 hours at 37°C. Culture supernatants were collected and then assayed to determine concentrations of interleukin-8 (IL-8) and macrophage inflammatory protein-1α (MIP-1α) by ELISA. RESULTS : We found that GBS stimulated Chorion Cell production of MIP-1α in a strain-specific fashion. We also found that both lipoteichoic acid and sialic acid stimulated concentration-dependent increases in Chorion Cell IL-8 production. Chorion Cells, however, did not increase MIP-1α production in response to either lipoteichoic acid or sialic acid. Two strains of GBS tested induced concentration-dependent increases in both IL-8 and MIP-1α, but both stimulated IL-8 production to a greater extent. Similarly, IL-1β also caused Chorion Cells to produce more IL-8 than MIP-1α. CONCLUSIONS : Our data are the first to show that GBS and purified bacterial products can stimulate chemokine production by fetal gestational tissues. We suggest that Chorion Cells may produce specific types of chemokines to attract different types of inflammatory Cells and thus may participate in the pathophysiology of infection-mediated preterm labor by directing specific inflammatory responses.
Murray D Mitchell - One of the best experts on this subject based on the ideXlab platform.
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regulation of cultured human Chorion Cell chemokine production by group b streptococci and purified bacterial products
American Journal of Reproductive Immunology, 1996Co-Authors: Donald J Dudley, Samuel S Edwin, Albina Dangerfield, Jennifer Van Waggoner, Murray D MitchellAbstract:PROBLEM : To determine if different strains of group B streptococci (GBS) and purified bacterial products regulate chemokine production by cultured human Chorion Cells. METHOD OF STUDY : Primary cultures of human Chorion Cells were established from placentae isolated from normal women at term gestation having repeat cesarean section. Five different strains of heat-killed GBS were incubated with confluent Chorion Cells at 10 7 bacteria/ml for 16 hours at 37°C. In separate experiments, lipoteichoic acid and sialic acid at various concentrations were incubated with Chorion Cells for 16 hours at 37°C. Culture supernatants were collected and then assayed to determine concentrations of interleukin-8 (IL-8) and macrophage inflammatory protein-1α (MIP-1α) by ELISA. RESULTS : We found that GBS stimulated Chorion Cell production of MIP-1α in a strain-specific fashion. We also found that both lipoteichoic acid and sialic acid stimulated concentration-dependent increases in Chorion Cell IL-8 production. Chorion Cells, however, did not increase MIP-1α production in response to either lipoteichoic acid or sialic acid. Two strains of GBS tested induced concentration-dependent increases in both IL-8 and MIP-1α, but both stimulated IL-8 production to a greater extent. Similarly, IL-1β also caused Chorion Cells to produce more IL-8 than MIP-1α. CONCLUSIONS : Our data are the first to show that GBS and purified bacterial products can stimulate chemokine production by fetal gestational tissues. We suggest that Chorion Cells may produce specific types of chemokines to attract different types of inflammatory Cells and thus may participate in the pathophysiology of infection-mediated preterm labor by directing specific inflammatory responses.
Hiroo Toyoda - One of the best experts on this subject based on the ideXlab platform.
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Differences in Permissive Cytomegalovirus Infection between Primary Cultured Human Fetal Membrane Chorion and Amnion Cells
Biological & Pharmaceutical Bulletin, 2013Co-Authors: Shigehiro Matsunaga, Noboru Uchide, Midori Shono, Kunio Ohyama, Makoto Takeichi, Hiroo ToyodaAbstract:Human cytomegalovirus (CMV) is the most common cause of viral intrauterine infection in developed countries. It has been shown that CMV DNA was frequently detected in the fetal membranes when the placenta was infected in utero. However, it is still not clear whether CMV replicates in constituent Cells of the fetal membranes. We investigated CMV infection of primary cultured Chorion and amnion Cells prepared from human fetal membrane tissues. In both types of Cell cultures, rounded Cells were observed at day 8 and 12 after CMV inoculation, and virus yields in culture supernatants were increased after the inoculation. In both types of Cells, viral immediately early 1 (IE1) protein-positive nuclei were scattered at day 4 after the inoculation, and IE1 mRNA was expressed throughout day 1 to 12 after CMV inoculation. In Chorion Cell cultures, the number of IE1 protein-positive nuclei increased significantly at day 8 and 12 after CMV inoculation as compared to day 4, by which foci were formed. Furthermore, an evident increase in levels of lactate dehydrogenase leakage from Chorion Cells was observed after CMV inoculation. Contrary, these phenomena were not observed in amnion Cell cultures. These results demonstrated that both Chorion and amnion Cells were permissive to CMV infection, while the velocity of Cell-to-Cell spread of CMV infection in amnion Cells was much lower than that in Chorion Cells. Therefore, the present study suggests that CMV may replicate rapidly in the Chorion Cell layer and slowly in the amnion Cell layer during intrauterine infection.
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Current Status of Monocyte Differentiation-Inducing (MDI) Factors Derived from Human Fetal Membrane Chorion Cells Undergoing Apoptosis after Influenza Virus Infection
Gene regulation and systems biology, 2007Co-Authors: Noboru Uchide, Hiroo ToyodaAbstract:Influenza virus infection induces apoptosis and the expression of a set of pro-inflammatory cytokine genes, such as interleukin (IL)-6, tumor necrosis factor (TNF)-α, interferon (IFN)-β and IFN-γ, in cultured human fetal membrane Chorion Cells. Monocyte differentiation-inducing (MDI) activity in culture supernatants is simultaneously increased by the virus infection. The MDI activity is predominantly influenced by IL-6 molecule in culture supernatants, and partly by TNF-α and IFN-β, but not IFN-γ, molecules. The MDI factors are able to induce the mRNA expression of macrophage class A scavenger receptor (SR-A), which is one of adhesion and apoptotic Cell-recognizing molecules, and gp91phox, which is a catalytic subunit of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme complex, on monocytic Cells. As a result, monocytes are initiated to differentiate into well-matured macrophages capable of adhering and producing superoxide through NADPH oxidase. The matured macrophages, obtained from human monocytic leukemia THP-1 Cells by the treatment with MDI factors, phagocytose apoptotic Chorion Cell debris resulting from the virus infection. Subsequent to phagocytosis, an abrupt increase of superoxide production by macrophages may occur. In this article, we summarize recent knowledge about the MDI factors derived from human fetal membrane Chorion Cells undergoing apoptosis after influenza virus infection, and discuss their possible pathological roles during pregnancy.
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Characterization of monocyte differentiation-inducing (MDI) factors derived from human fetal membrane Chorion Cells undergoing apoptosis after influenza virus infection
The International Journal of Biochemistry & Cell Biology, 2006Co-Authors: Noboru Uchide, Kunio Ohyama, Chiho Tadera, Hiroko Sarai, Toshio Bessho, Hiroo ToyodaAbstract:Abstract Influenza virus infection during pregnancy has been implicated as one of cause of premature delivery, abortion and stillbirth. We have reported that cultured human fetal membrane Chorion Cells undergoing apoptosis by influenza virus infection secrete unidentified heat-stable monocyte differentiation-inducing (MDI) factors. In this study, Cellular, biological and immunochemical characteristics of MDI factors were investigated using human monocytic leukemia THP-1 Cells by nitroblue tetrazolium reduction and Cell adhesion assays. The treatment of THP-1 Cells with culture supernatants from the influenza virus-infected Chorion Cells induced the nitroblue tetrazolium reduction ability, which was inhibited by the addition of superoxide dismutase and diphenyleneiodonium chloride, an inhibitor for reduced nicotinamide adenine dinucleotide phosphate oxidase. The phenomenon was also observed in human peripheral blood monocytes and histiocytic leukemia U937 Cells, but not in promyelocytic leukemia HL-60 Cells. The induction of nitroblue tetrazolium reduction and adhesion abilities in THP-1 Cells was closely correlated with the concentrations of interleukin-6 protein in the culture supernatants. These abilities were inhibited to approximately 60% by the addition of antibodies against interleukin-6, or α-chain (gp80) or β-chain (gp130) of IL-6 receptor. The induction of nitroblue tetrazolium reduction was increased by the addition of supernatants from amnioChorion tissue cultures after influenza virus infection. These results indicate that Chorion Cell-derived interleukin-6 is partly responsible for monocyte differentiation to macrophages capable of generating superoxide anion. It is possible that these pathways represent part of the mechanism for birth complications associated with intrauterine influenza infection in pregnancy.