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Christopher J. Lucarotti - One of the best experts on this subject based on the ideXlab platform.

  • Comparative Genome Sequence Analysis of Choristoneura occidentalis Freeman and C. rosaceana
    2016
    Co-Authors: David K. Thumbi, Michel Cusson, Christopher J. Lucarotti
    Abstract:

    The complete genome sequences of Choristoneura occidentalis and C. rosaceana nucleopolyhedroviruses (ChocNPV and ChroNPV, respectively) (Baculoviridae: Alphabaculovirus) were determined and compared with each other and with those of other baculoviruses, including the genome of the closely related C. fumiferana NPV (CfMNPV). The ChocNPV genome was 128,446 bp in length (1147 bp smaller than that of CfMNPV), had a G+C content of 50.1%, and contained 148 open reading frames (ORFs). In comparison, the ChroNPV genome was 129,052 bp in length, had a G+C content of 48.6 % and contained 149 ORFs. ChocNPV and ChroNPV shared 144 ORFs in common, and had a 77 % sequence identity with each other and 96.5 % and 77.8 % sequence identity, respectively, with CfMNPV. Five homologous regions (hrs), with sequence similarities to those of CfMNPV, were identified in ChocNPV, whereas the ChroNPV genome contained three hrs featuring up to 14 repeats. Both genomes encoded three inhibitors of apoptosis (IAP-1, IAP-2, and IAP-3), as reported for CfMNPV, and the ChocNPV IAP-3 gene represented the most divergent functional region of this genome relative to CfMNPV. Two ORFs were unique to ChocNPV, and four were unique to ChroNPV. ChroNPV ORF chronpv38 is a eukaryotic initiation factor 5 (eIF-5) homolog that has also been identified in the C. occidentalis granulovirus (ChocGV) and is believed to be the product of horizontal gene transfer from the host. Based on levels of sequence identity and phylogenetic analysis, both ChocNPV and ChroNPV fall within group I alphabaculoviruses, where ChocNPV appears to be more closely related to CfMNPV than doe

  • Baculoviruses in populations of western spruce budworm.
    Journal of Invertebrate Pathology, 2015
    Co-Authors: Vincent G. Nealis, Robert I. Graham, Benoit Morin, R. Turnquist, Christopher J. Lucarotti
    Abstract:

    Population studies of western spruce budworm, Choristoneura occidentalis, revealed that a baculovirus, ChocNPV, was widespread in outbreak populations over a broad geographical area of British Columbia, Canada although the rate of mortality was usually low (

  • comparative genome sequence analysis of Choristoneura occidentalis freeman and c rosaceana harris lepidoptera tortricidae alphabaculoviruses
    PLOS ONE, 2013
    Co-Authors: David K. Thumbi, Renée Lapointe, Michel Cusson, Catherine Béliveau, Christopher J. Lucarotti
    Abstract:

    The complete genome sequences of Choristoneura occidentalis and C. rosaceana nucleopolyhedroviruses (ChocNPV and ChroNPV, respectively) (Baculoviridae: Alphabaculovirus) were determined and compared with each other and with those of other baculoviruses, including the genome of the closely related C. fumiferana NPV (CfMNPV). The ChocNPV genome was 128,446 bp in length (1147 bp smaller than that of CfMNPV), had a G+C content of 50.1%, and contained 148 open reading frames (ORFs). In comparison, the ChroNPV genome was 129,052 bp in length, had a G+C content of 48.6% and contained 149 ORFs. ChocNPV and ChroNPV shared 144 ORFs in common, and had a 77% sequence identity with each other and 96.5% and 77.8% sequence identity, respectively, with CfMNPV. Five homologous regions (hrs), with sequence similarities to those of CfMNPV, were identified in ChocNPV, whereas the ChroNPV genome contained three hrs featuring up to 14 repeats. Both genomes encoded three inhibitors of apoptosis (IAP-1, IAP-2, and IAP-3), as reported for CfMNPV, and the ChocNPV IAP-3 gene represented the most divergent functional region of this genome relative to CfMNPV. Two ORFs were unique to ChocNPV, and four were unique to ChroNPV. ChroNPV ORF chronpv38 is a eukaryotic initiation factor 5 (eIF-5) homolog that has also been identified in the C. occidentalis granulovirus (ChocGV) and is believed to be the product of horizontal gene transfer from the host. Based on levels of sequence identity and phylogenetic analysis, both ChocNPV and ChroNPV fall within group I alphabaculoviruses, where ChocNPV appears to be more closely related to CfMNPV than does ChroNPV. Our analyses suggest that it may be appropriate to consider ChocNPV and CfMNPV as variants of the same virus species.

  • Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis
    Archives of Virology, 2008
    Co-Authors: Robert I. Graham, Vincent G. Nealis, Renée Lapointe, Benoit Morin, Christopher J. Lucarotti
    Abstract:

    A novel cypovirus, assigned CoCPV, was isolated from natural populations of the western spruce budworm, Choristoneura occidentalis . The complete nucleotide sequences of genomic segments S2–S5 and S7–S10 were determined. Each segment contained a single open reading frame. Conserved motifs 5′ (AGUUU……UUUGUGC) 3′ were found at the ends of each segment. Analysis of S2, which encoded a putative RNA-dependent RNA polymerase protein, confirmed CoCPV belonged to the genus Cypovirus within the family Reoviridae . Further phylogenetic analysis using S10 (the polyhedrin gene) aligned this virus with species type-16, closely related to a cypovirus isolated from C. fumiferana .

Robert I. Graham - One of the best experts on this subject based on the ideXlab platform.

  • Baculoviruses in populations of western spruce budworm.
    Journal of Invertebrate Pathology, 2015
    Co-Authors: Vincent G. Nealis, Robert I. Graham, Benoit Morin, R. Turnquist, Christopher J. Lucarotti
    Abstract:

    Population studies of western spruce budworm, Choristoneura occidentalis, revealed that a baculovirus, ChocNPV, was widespread in outbreak populations over a broad geographical area of British Columbia, Canada although the rate of mortality was usually low (

  • Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis
    Archives of Virology, 2008
    Co-Authors: Robert I. Graham, Vincent G. Nealis, Renée Lapointe, Benoit Morin, Christopher J. Lucarotti
    Abstract:

    A novel cypovirus, assigned CoCPV, was isolated from natural populations of the western spruce budworm, Choristoneura occidentalis . The complete nucleotide sequences of genomic segments S2–S5 and S7–S10 were determined. Each segment contained a single open reading frame. Conserved motifs 5′ (AGUUU……UUUGUGC) 3′ were found at the ends of each segment. Analysis of S2, which encoded a putative RNA-dependent RNA polymerase protein, confirmed CoCPV belonged to the genus Cypovirus within the family Reoviridae . Further phylogenetic analysis using S10 (the polyhedrin gene) aligned this virus with species type-16, closely related to a cypovirus isolated from C. fumiferana .

Vicky J. Erickson - One of the best experts on this subject based on the ideXlab platform.

  • Carry-over effects of trunk-implanted acephate for protecting Douglas fir from western-spruce-budworm defoliation
    Forest Ecology and Management, 1991
    Co-Authors: Roger E. Sandquist, Vicky J. Erickson
    Abstract:

    Abstract Carry-over effects of the systemic insecticide acephate (O, S-dimethyl acetylphosphoramidothioate) were evaluated 1 year after Douglas-fir, Pseudotsuga menziesii var. glauca (Beissn.) Franco, were implanted. Trees had protection from western spruce budworm, Choristoneura occidentalis (Freeman) (Lepidoptera: Tortricidae), the year after treatment. Foliage protection with implants installed 1 week after vegetative bud-burst was similar to its protection the first year. Foliage protection with implants installed 3 weeks before vegetative bud-burst was less than its protection the first year, less than the later implants, and slightly more than untreated controls.

Vincent G. Nealis - One of the best experts on this subject based on the ideXlab platform.

  • Baculoviruses in populations of western spruce budworm.
    Journal of Invertebrate Pathology, 2015
    Co-Authors: Vincent G. Nealis, Robert I. Graham, Benoit Morin, R. Turnquist, Christopher J. Lucarotti
    Abstract:

    Population studies of western spruce budworm, Choristoneura occidentalis, revealed that a baculovirus, ChocNPV, was widespread in outbreak populations over a broad geographical area of British Columbia, Canada although the rate of mortality was usually low (

  • Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis
    Archives of Virology, 2008
    Co-Authors: Robert I. Graham, Vincent G. Nealis, Renée Lapointe, Benoit Morin, Christopher J. Lucarotti
    Abstract:

    A novel cypovirus, assigned CoCPV, was isolated from natural populations of the western spruce budworm, Choristoneura occidentalis . The complete nucleotide sequences of genomic segments S2–S5 and S7–S10 were determined. Each segment contained a single open reading frame. Conserved motifs 5′ (AGUUU……UUUGUGC) 3′ were found at the ends of each segment. Analysis of S2, which encoded a putative RNA-dependent RNA polymerase protein, confirmed CoCPV belonged to the genus Cypovirus within the family Reoviridae . Further phylogenetic analysis using S10 (the polyhedrin gene) aligned this virus with species type-16, closely related to a cypovirus isolated from C. fumiferana .

Benoit Morin - One of the best experts on this subject based on the ideXlab platform.

  • Baculoviruses in populations of western spruce budworm.
    Journal of Invertebrate Pathology, 2015
    Co-Authors: Vincent G. Nealis, Robert I. Graham, Benoit Morin, R. Turnquist, Christopher J. Lucarotti
    Abstract:

    Population studies of western spruce budworm, Choristoneura occidentalis, revealed that a baculovirus, ChocNPV, was widespread in outbreak populations over a broad geographical area of British Columbia, Canada although the rate of mortality was usually low (

  • Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis
    Archives of Virology, 2008
    Co-Authors: Robert I. Graham, Vincent G. Nealis, Renée Lapointe, Benoit Morin, Christopher J. Lucarotti
    Abstract:

    A novel cypovirus, assigned CoCPV, was isolated from natural populations of the western spruce budworm, Choristoneura occidentalis . The complete nucleotide sequences of genomic segments S2–S5 and S7–S10 were determined. Each segment contained a single open reading frame. Conserved motifs 5′ (AGUUU……UUUGUGC) 3′ were found at the ends of each segment. Analysis of S2, which encoded a putative RNA-dependent RNA polymerase protein, confirmed CoCPV belonged to the genus Cypovirus within the family Reoviridae . Further phylogenetic analysis using S10 (the polyhedrin gene) aligned this virus with species type-16, closely related to a cypovirus isolated from C. fumiferana .