The Experts below are selected from a list of 1404 Experts worldwide ranked by ideXlab platform

Takashi Yoshimoto - One of the best experts on this subject based on the ideXlab platform.

  • Production and Secretion of Adrenomedullin by Cultured Choroid Plexus Carcinoma Cells
    Journal of neurochemistry, 2002
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Takashi Yoshimoto, Shigeki Shibahara
    Abstract:

    Adrenomedullin is a potent vasodilator peptide that was originally isolated from pheochromocytoma. The production and secretion of adrenomedullin by cultured Choroid Plexus Carcinoma cells were studied by radioimmunoassay and northern blot hybridization. Choroid Plexus Carcinoma is a rare malignant tumor derived from the epithelium of the Choroid Plexus. Immunoreactive adrenomedullin was detected in the conditioned medium of Choroid Plexus Carcinoma cells (40.8 +/- 7.5 fmol/10(5) cells/24 h; mean +/- SEM, n = 5). Reverse-phase HPLC of the conditioned medium showed one major peak of the immunoreactive peptide eluting in the position of synthetic human adrenomedullin and two smaller peaks eluting earlier. Addition of interleukin-1 beta (10 ng/ml) alone or in combination with three cytokines, interferon-gamma (100 U/ml), tumor necrosis factor-alpha (20 ng/ml), and interleukin-1 beta (10 ng/ml), caused significant increases in the immunoreactive adrenomedullin concentrations in the medium (approximately 175 and 293% of the control level, respectively). Northern blot analysis showed the expression of 1.6-kb adrenomedullin mRNA in the total RNA sample prepared from cultured Choroid Plexus Carcinoma cells. Treatment with either interleukin-1 beta or the combination of three cytokines caused significant increases in levels of adrenomedullin mRNA in parallel with those in immunoreactive adrenomedullin concentrations in the conditioned medium. These findings raise a possibility that adrenomedullin is secreted from the Choroid Plexus and has physiological roles in the CNS via the CSF. In addition, adrenomedullin secreted from Choroid Plexus Carcinoma may be related to the pathophysiology of the tumor.

  • Production and secretion of endothelin-1 by cultured Choroid Plexus Carcinoma cells
    Journal of Cardiovascular Pharmacology, 1998
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Kazuhito Totsune, Masahiko Sone, Takashi Yoshimoto
    Abstract:

    Choroid Plexus Carcinoma is a rare neoplasm derived from the epithelium of the Choroid Plexus. The production and secretion of endothelin-1 (ET-1) by cultured human Choroid Plexus Carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 ± 0.12 fmol/10 5 cells/24 h; n = 5: mean ± SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) or a combination of interferon-γ (IFN-γ), TNF-α, and IL-1β caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-I mRNA in Choroid Plexus Carcinoma cells. The expression levels of ET-I mRNA were increased by treatment with a combination of IFN-γ TNF-α, and IL-1β. The present study has shown the production and secretion of ET- I by cultured human Choroid Plexus Carcinoma cells and suggests the possibility that ET- I formation is related to the pathophysiology of this tumor.

  • Production and secretion of endothelin-1 by cultured Choroid Plexus Carcinoma cells.
    Journal of cardiovascular pharmacology, 1998
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Kazuhito Totsune, Masahiko Sone, Takashi Yoshimoto
    Abstract:

    Choroid Plexus Carcinoma is a rare neoplasm derived from the epithelium of the Choroid Plexus. The production and secretion of endothelin-1 (ET-1) by cultured human Choroid Plexus Carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 +/- 0.12 fmol/10(5) cells/24 h; n = 5; mean +/- SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) or a combination of interferon-gamma (IFN-gamma), TNF-alpha, and IL-1 beta caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-1 mRNA in Choroid Plexus Carcinoma cells. The expression levels of ET-1 mRNA were increased by treatment with a combination of IFN-gamma, TNF-alpha, and IL-1 beta. The present study has shown the production and secretion of ET-1 by cultured human Choroid Plexus Carcinoma cells and suggests the possibility that ET-1 formation is related to the pathophysiology of this tumor.

  • Intraoperative radiation therapy and chemotherapy for huge Choroid Plexus Carcinoma in an infant--case report.
    Neurologia medico-chirurgica, 1996
    Co-Authors: Toshihiro Kumabe, Teiji Tominaga, Takashi Yoshimoto, Takeo Kondo, Akamasa Kayama
    Abstract:

    A 2-month-old boy presented with a huge Choroid Plexus Carcinoma in the left cerebral hemisphere. Almost total resection of the tumor was achieved, but a remnant abruptly enlarged 5 months later, requiring reoperation. After radical resection of the tumor, intraoperative radiation (10 Gy) was delivered. Postoperative chemotherapy was performed with methotrexate and vincristine, selected on the basis of in vitro chemosensitivity testing. No recurrence of the tumor was detected 18 months after the second operation. Radical surgery with these adjuvant therapies may be a good method to control this aggressive disease in infants.

Kazuhiro Takahashi - One of the best experts on this subject based on the ideXlab platform.

  • Production and Secretion of Adrenomedullin by Cultured Choroid Plexus Carcinoma Cells
    Journal of neurochemistry, 2002
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Takashi Yoshimoto, Shigeki Shibahara
    Abstract:

    Adrenomedullin is a potent vasodilator peptide that was originally isolated from pheochromocytoma. The production and secretion of adrenomedullin by cultured Choroid Plexus Carcinoma cells were studied by radioimmunoassay and northern blot hybridization. Choroid Plexus Carcinoma is a rare malignant tumor derived from the epithelium of the Choroid Plexus. Immunoreactive adrenomedullin was detected in the conditioned medium of Choroid Plexus Carcinoma cells (40.8 +/- 7.5 fmol/10(5) cells/24 h; mean +/- SEM, n = 5). Reverse-phase HPLC of the conditioned medium showed one major peak of the immunoreactive peptide eluting in the position of synthetic human adrenomedullin and two smaller peaks eluting earlier. Addition of interleukin-1 beta (10 ng/ml) alone or in combination with three cytokines, interferon-gamma (100 U/ml), tumor necrosis factor-alpha (20 ng/ml), and interleukin-1 beta (10 ng/ml), caused significant increases in the immunoreactive adrenomedullin concentrations in the medium (approximately 175 and 293% of the control level, respectively). Northern blot analysis showed the expression of 1.6-kb adrenomedullin mRNA in the total RNA sample prepared from cultured Choroid Plexus Carcinoma cells. Treatment with either interleukin-1 beta or the combination of three cytokines caused significant increases in levels of adrenomedullin mRNA in parallel with those in immunoreactive adrenomedullin concentrations in the conditioned medium. These findings raise a possibility that adrenomedullin is secreted from the Choroid Plexus and has physiological roles in the CNS via the CSF. In addition, adrenomedullin secreted from Choroid Plexus Carcinoma may be related to the pathophysiology of the tumor.

  • Production and secretion of endothelin-1 by cultured Choroid Plexus Carcinoma cells
    Journal of Cardiovascular Pharmacology, 1998
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Kazuhito Totsune, Masahiko Sone, Takashi Yoshimoto
    Abstract:

    Choroid Plexus Carcinoma is a rare neoplasm derived from the epithelium of the Choroid Plexus. The production and secretion of endothelin-1 (ET-1) by cultured human Choroid Plexus Carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 ± 0.12 fmol/10 5 cells/24 h; n = 5: mean ± SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) or a combination of interferon-γ (IFN-γ), TNF-α, and IL-1β caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-I mRNA in Choroid Plexus Carcinoma cells. The expression levels of ET-I mRNA were increased by treatment with a combination of IFN-γ TNF-α, and IL-1β. The present study has shown the production and secretion of ET- I by cultured human Choroid Plexus Carcinoma cells and suggests the possibility that ET- I formation is related to the pathophysiology of this tumor.

  • Production and secretion of endothelin-1 by cultured Choroid Plexus Carcinoma cells.
    Journal of cardiovascular pharmacology, 1998
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Kazuhito Totsune, Masahiko Sone, Takashi Yoshimoto
    Abstract:

    Choroid Plexus Carcinoma is a rare neoplasm derived from the epithelium of the Choroid Plexus. The production and secretion of endothelin-1 (ET-1) by cultured human Choroid Plexus Carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 +/- 0.12 fmol/10(5) cells/24 h; n = 5; mean +/- SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) or a combination of interferon-gamma (IFN-gamma), TNF-alpha, and IL-1 beta caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-1 mRNA in Choroid Plexus Carcinoma cells. The expression levels of ET-1 mRNA were increased by treatment with a combination of IFN-gamma, TNF-alpha, and IL-1 beta. The present study has shown the production and secretion of ET-1 by cultured human Choroid Plexus Carcinoma cells and suggests the possibility that ET-1 formation is related to the pathophysiology of this tumor.

Toshihiro Kumabe - One of the best experts on this subject based on the ideXlab platform.

  • Production and Secretion of Adrenomedullin by Cultured Choroid Plexus Carcinoma Cells
    Journal of neurochemistry, 2002
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Takashi Yoshimoto, Shigeki Shibahara
    Abstract:

    Adrenomedullin is a potent vasodilator peptide that was originally isolated from pheochromocytoma. The production and secretion of adrenomedullin by cultured Choroid Plexus Carcinoma cells were studied by radioimmunoassay and northern blot hybridization. Choroid Plexus Carcinoma is a rare malignant tumor derived from the epithelium of the Choroid Plexus. Immunoreactive adrenomedullin was detected in the conditioned medium of Choroid Plexus Carcinoma cells (40.8 +/- 7.5 fmol/10(5) cells/24 h; mean +/- SEM, n = 5). Reverse-phase HPLC of the conditioned medium showed one major peak of the immunoreactive peptide eluting in the position of synthetic human adrenomedullin and two smaller peaks eluting earlier. Addition of interleukin-1 beta (10 ng/ml) alone or in combination with three cytokines, interferon-gamma (100 U/ml), tumor necrosis factor-alpha (20 ng/ml), and interleukin-1 beta (10 ng/ml), caused significant increases in the immunoreactive adrenomedullin concentrations in the medium (approximately 175 and 293% of the control level, respectively). Northern blot analysis showed the expression of 1.6-kb adrenomedullin mRNA in the total RNA sample prepared from cultured Choroid Plexus Carcinoma cells. Treatment with either interleukin-1 beta or the combination of three cytokines caused significant increases in levels of adrenomedullin mRNA in parallel with those in immunoreactive adrenomedullin concentrations in the conditioned medium. These findings raise a possibility that adrenomedullin is secreted from the Choroid Plexus and has physiological roles in the CNS via the CSF. In addition, adrenomedullin secreted from Choroid Plexus Carcinoma may be related to the pathophysiology of the tumor.

  • Production and secretion of endothelin-1 by cultured Choroid Plexus Carcinoma cells
    Journal of Cardiovascular Pharmacology, 1998
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Kazuhito Totsune, Masahiko Sone, Takashi Yoshimoto
    Abstract:

    Choroid Plexus Carcinoma is a rare neoplasm derived from the epithelium of the Choroid Plexus. The production and secretion of endothelin-1 (ET-1) by cultured human Choroid Plexus Carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 ± 0.12 fmol/10 5 cells/24 h; n = 5: mean ± SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) or a combination of interferon-γ (IFN-γ), TNF-α, and IL-1β caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-I mRNA in Choroid Plexus Carcinoma cells. The expression levels of ET-I mRNA were increased by treatment with a combination of IFN-γ TNF-α, and IL-1β. The present study has shown the production and secretion of ET- I by cultured human Choroid Plexus Carcinoma cells and suggests the possibility that ET- I formation is related to the pathophysiology of this tumor.

  • Production and secretion of endothelin-1 by cultured Choroid Plexus Carcinoma cells.
    Journal of cardiovascular pharmacology, 1998
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Kazuhito Totsune, Masahiko Sone, Takashi Yoshimoto
    Abstract:

    Choroid Plexus Carcinoma is a rare neoplasm derived from the epithelium of the Choroid Plexus. The production and secretion of endothelin-1 (ET-1) by cultured human Choroid Plexus Carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 +/- 0.12 fmol/10(5) cells/24 h; n = 5; mean +/- SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) or a combination of interferon-gamma (IFN-gamma), TNF-alpha, and IL-1 beta caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-1 mRNA in Choroid Plexus Carcinoma cells. The expression levels of ET-1 mRNA were increased by treatment with a combination of IFN-gamma, TNF-alpha, and IL-1 beta. The present study has shown the production and secretion of ET-1 by cultured human Choroid Plexus Carcinoma cells and suggests the possibility that ET-1 formation is related to the pathophysiology of this tumor.

  • Intraoperative radiation therapy and chemotherapy for huge Choroid Plexus Carcinoma in an infant--case report.
    Neurologia medico-chirurgica, 1996
    Co-Authors: Toshihiro Kumabe, Teiji Tominaga, Takashi Yoshimoto, Takeo Kondo, Akamasa Kayama
    Abstract:

    A 2-month-old boy presented with a huge Choroid Plexus Carcinoma in the left cerebral hemisphere. Almost total resection of the tumor was achieved, but a remnant abruptly enlarged 5 months later, requiring reoperation. After radical resection of the tumor, intraoperative radiation (10 Gy) was delivered. Postoperative chemotherapy was performed with methotrexate and vincristine, selected on the basis of in vitro chemosensitivity testing. No recurrence of the tumor was detected 18 months after the second operation. Radical surgery with these adjuvant therapies may be a good method to control this aggressive disease in infants.

Teiji Tominaga - One of the best experts on this subject based on the ideXlab platform.

  • Production and Secretion of Adrenomedullin by Cultured Choroid Plexus Carcinoma Cells
    Journal of neurochemistry, 2002
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Takashi Yoshimoto, Shigeki Shibahara
    Abstract:

    Adrenomedullin is a potent vasodilator peptide that was originally isolated from pheochromocytoma. The production and secretion of adrenomedullin by cultured Choroid Plexus Carcinoma cells were studied by radioimmunoassay and northern blot hybridization. Choroid Plexus Carcinoma is a rare malignant tumor derived from the epithelium of the Choroid Plexus. Immunoreactive adrenomedullin was detected in the conditioned medium of Choroid Plexus Carcinoma cells (40.8 +/- 7.5 fmol/10(5) cells/24 h; mean +/- SEM, n = 5). Reverse-phase HPLC of the conditioned medium showed one major peak of the immunoreactive peptide eluting in the position of synthetic human adrenomedullin and two smaller peaks eluting earlier. Addition of interleukin-1 beta (10 ng/ml) alone or in combination with three cytokines, interferon-gamma (100 U/ml), tumor necrosis factor-alpha (20 ng/ml), and interleukin-1 beta (10 ng/ml), caused significant increases in the immunoreactive adrenomedullin concentrations in the medium (approximately 175 and 293% of the control level, respectively). Northern blot analysis showed the expression of 1.6-kb adrenomedullin mRNA in the total RNA sample prepared from cultured Choroid Plexus Carcinoma cells. Treatment with either interleukin-1 beta or the combination of three cytokines caused significant increases in levels of adrenomedullin mRNA in parallel with those in immunoreactive adrenomedullin concentrations in the conditioned medium. These findings raise a possibility that adrenomedullin is secreted from the Choroid Plexus and has physiological roles in the CNS via the CSF. In addition, adrenomedullin secreted from Choroid Plexus Carcinoma may be related to the pathophysiology of the tumor.

  • Production and secretion of endothelin-1 by cultured Choroid Plexus Carcinoma cells
    Journal of Cardiovascular Pharmacology, 1998
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Kazuhito Totsune, Masahiko Sone, Takashi Yoshimoto
    Abstract:

    Choroid Plexus Carcinoma is a rare neoplasm derived from the epithelium of the Choroid Plexus. The production and secretion of endothelin-1 (ET-1) by cultured human Choroid Plexus Carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 ± 0.12 fmol/10 5 cells/24 h; n = 5: mean ± SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) or a combination of interferon-γ (IFN-γ), TNF-α, and IL-1β caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-I mRNA in Choroid Plexus Carcinoma cells. The expression levels of ET-I mRNA were increased by treatment with a combination of IFN-γ TNF-α, and IL-1β. The present study has shown the production and secretion of ET- I by cultured human Choroid Plexus Carcinoma cells and suggests the possibility that ET- I formation is related to the pathophysiology of this tumor.

  • Production and secretion of endothelin-1 by cultured Choroid Plexus Carcinoma cells.
    Journal of cardiovascular pharmacology, 1998
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Kazuhito Totsune, Masahiko Sone, Takashi Yoshimoto
    Abstract:

    Choroid Plexus Carcinoma is a rare neoplasm derived from the epithelium of the Choroid Plexus. The production and secretion of endothelin-1 (ET-1) by cultured human Choroid Plexus Carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 +/- 0.12 fmol/10(5) cells/24 h; n = 5; mean +/- SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) or a combination of interferon-gamma (IFN-gamma), TNF-alpha, and IL-1 beta caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-1 mRNA in Choroid Plexus Carcinoma cells. The expression levels of ET-1 mRNA were increased by treatment with a combination of IFN-gamma, TNF-alpha, and IL-1 beta. The present study has shown the production and secretion of ET-1 by cultured human Choroid Plexus Carcinoma cells and suggests the possibility that ET-1 formation is related to the pathophysiology of this tumor.

  • Intraoperative radiation therapy and chemotherapy for huge Choroid Plexus Carcinoma in an infant--case report.
    Neurologia medico-chirurgica, 1996
    Co-Authors: Toshihiro Kumabe, Teiji Tominaga, Takashi Yoshimoto, Takeo Kondo, Akamasa Kayama
    Abstract:

    A 2-month-old boy presented with a huge Choroid Plexus Carcinoma in the left cerebral hemisphere. Almost total resection of the tumor was achieved, but a remnant abruptly enlarged 5 months later, requiring reoperation. After radical resection of the tumor, intraoperative radiation (10 Gy) was delivered. Postoperative chemotherapy was performed with methotrexate and vincristine, selected on the basis of in vitro chemosensitivity testing. No recurrence of the tumor was detected 18 months after the second operation. Radical surgery with these adjuvant therapies may be a good method to control this aggressive disease in infants.

Fumitoshi Satoh - One of the best experts on this subject based on the ideXlab platform.

  • Production and Secretion of Adrenomedullin by Cultured Choroid Plexus Carcinoma Cells
    Journal of neurochemistry, 2002
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Takashi Yoshimoto, Shigeki Shibahara
    Abstract:

    Adrenomedullin is a potent vasodilator peptide that was originally isolated from pheochromocytoma. The production and secretion of adrenomedullin by cultured Choroid Plexus Carcinoma cells were studied by radioimmunoassay and northern blot hybridization. Choroid Plexus Carcinoma is a rare malignant tumor derived from the epithelium of the Choroid Plexus. Immunoreactive adrenomedullin was detected in the conditioned medium of Choroid Plexus Carcinoma cells (40.8 +/- 7.5 fmol/10(5) cells/24 h; mean +/- SEM, n = 5). Reverse-phase HPLC of the conditioned medium showed one major peak of the immunoreactive peptide eluting in the position of synthetic human adrenomedullin and two smaller peaks eluting earlier. Addition of interleukin-1 beta (10 ng/ml) alone or in combination with three cytokines, interferon-gamma (100 U/ml), tumor necrosis factor-alpha (20 ng/ml), and interleukin-1 beta (10 ng/ml), caused significant increases in the immunoreactive adrenomedullin concentrations in the medium (approximately 175 and 293% of the control level, respectively). Northern blot analysis showed the expression of 1.6-kb adrenomedullin mRNA in the total RNA sample prepared from cultured Choroid Plexus Carcinoma cells. Treatment with either interleukin-1 beta or the combination of three cytokines caused significant increases in levels of adrenomedullin mRNA in parallel with those in immunoreactive adrenomedullin concentrations in the conditioned medium. These findings raise a possibility that adrenomedullin is secreted from the Choroid Plexus and has physiological roles in the CNS via the CSF. In addition, adrenomedullin secreted from Choroid Plexus Carcinoma may be related to the pathophysiology of the tumor.

  • Production and secretion of endothelin-1 by cultured Choroid Plexus Carcinoma cells
    Journal of Cardiovascular Pharmacology, 1998
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Kazuhito Totsune, Masahiko Sone, Takashi Yoshimoto
    Abstract:

    Choroid Plexus Carcinoma is a rare neoplasm derived from the epithelium of the Choroid Plexus. The production and secretion of endothelin-1 (ET-1) by cultured human Choroid Plexus Carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 ± 0.12 fmol/10 5 cells/24 h; n = 5: mean ± SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) or a combination of interferon-γ (IFN-γ), TNF-α, and IL-1β caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-I mRNA in Choroid Plexus Carcinoma cells. The expression levels of ET-I mRNA were increased by treatment with a combination of IFN-γ TNF-α, and IL-1β. The present study has shown the production and secretion of ET- I by cultured human Choroid Plexus Carcinoma cells and suggests the possibility that ET- I formation is related to the pathophysiology of this tumor.

  • Production and secretion of endothelin-1 by cultured Choroid Plexus Carcinoma cells.
    Journal of cardiovascular pharmacology, 1998
    Co-Authors: Kazuhiro Takahashi, Fumitoshi Satoh, Eishi Hara, Osamu Murakami, Toshihiro Kumabe, Teiji Tominaga, Takamasa Kayama, Kazuhito Totsune, Masahiko Sone, Takashi Yoshimoto
    Abstract:

    Choroid Plexus Carcinoma is a rare neoplasm derived from the epithelium of the Choroid Plexus. The production and secretion of endothelin-1 (ET-1) by cultured human Choroid Plexus Carcinoma cells were studied by radioimmunoassay and Northern blot analysis. Immunoreactive (IR)-ET was detected in the culture medium (2.78 +/- 0.12 fmol/10(5) cells/24 h; n = 5; mean +/- SEM) but not in the unconditioned medium. Reverse-phase high-performance liquid chromatography of the extract of the culture medium showed a single peak eluting in the position of ET-1. Treatment with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) or a combination of interferon-gamma (IFN-gamma), TNF-alpha, and IL-1 beta caused significant increases in the IR-ET levels in the culture medium. Northern blot analysis of total RNA showed the expression of ET-1 mRNA in Choroid Plexus Carcinoma cells. The expression levels of ET-1 mRNA were increased by treatment with a combination of IFN-gamma, TNF-alpha, and IL-1 beta. The present study has shown the production and secretion of ET-1 by cultured human Choroid Plexus Carcinoma cells and suggests the possibility that ET-1 formation is related to the pathophysiology of this tumor.