The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform

Hai-qin Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Molecular cytogenetic characterization of wheat–Elymus repens Chromosomal Translocation lines with resistance to Fusarium head blight and stripe rust
    BMC plant biology, 2019
    Co-Authors: Biran Gong, J. Zeng, Wei Zhu, Li Sanyue, Yuqi Wang, Yi Wang, Xing Fan, Lina Sha, Hai-qin Zhang
    Abstract:

    Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe and stripe rust caused by Puccinia striiformis f. sp. tritici are devastating diseases that affect wheat production worldwide. The use of disease-resistant genes and cultivars is the most effective means of reducing fungicide applications to combat these diseases. Elymus repens (2n = 6x = 42, StStStStHH) is a potentially useful germplasm of FHB and stripe rust resistance for wheat improvement. Here, we report the development and characterization of two wheat–E. repens lines derived from the progeny of common wheat–E. repens hybrids. Cytological studies indicated that the mean chromosome configuration of K15–1192-2 and K15–1194-2 at meiosis were 2n = 42 = 0.86 I + 17.46 II (ring) + 3.11 II (rod) and 2n = 42 = 2.45 I + 14.17 II (ring) + 5.50 II (rod) + 0.07 III, respectively. Genomic and fluorescence in situ hybridization karyotyping and simple sequence repeats markers revealed that K15–1192-2 was a wheat–E. repens 3D/?St double terminal Chromosomal Translocation line. Line K15–1194-2 was identified as harboring a pair of 7DS/?StL Robertsonian Translocations and one 3D/?St double terminal Translocational chromosome. Further analyses using specific expressed sequence tag-SSR markers confirmed that the wheat–E. repens Translocations involved the 3St chromatin in both lines. Furthermore, compared with the wheat parent Chuannong16, K15–1192-2 and K15–1194-2 expressed high levels of resistance to FHB and stripe rust pathogens prevalent in China. Thus, this study has determined that the chromosome 3St of E. repens harbors gene(s) highly resistant to FHB and stripe rust, and chromatin of 3St introgressed into wheat chromosomes completely presented the resistance, indicating the feasibility of using these Translocation lines as novel material for breeding resistant wheat cultivars and alien gene mining.

  • molecular cytogenetic characterization of wheat elymus repens Chromosomal Translocation lines with resistance to fusarium head blight and stripe rust
    BMC Plant Biology, 2019
    Co-Authors: Biran Gong, J. Zeng, Wei Zhu, Yuqi Wang, Yi Wang, Xing Fan, Lina Sha, Hai-qin Zhang, Lin Huang, Guoyue Chen
    Abstract:

    Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe and stripe rust caused by Puccinia striiformis f. sp. tritici are devastating diseases that affect wheat production worldwide. The use of disease-resistant genes and cultivars is the most effective means of reducing fungicide applications to combat these diseases. Elymus repens (2n = 6x = 42, StStStStHH) is a potentially useful germplasm of FHB and stripe rust resistance for wheat improvement. Here, we report the development and characterization of two wheat–E. repens lines derived from the progeny of common wheat–E. repens hybrids. Cytological studies indicated that the mean chromosome configuration of K15–1192-2 and K15–1194-2 at meiosis were 2n = 42 = 0.86 I + 17.46 II (ring) + 3.11 II (rod) and 2n = 42 = 2.45 I + 14.17 II (ring) + 5.50 II (rod) + 0.07 III, respectively. Genomic and fluorescence in situ hybridization karyotyping and simple sequence repeats markers revealed that K15–1192-2 was a wheat–E. repens 3D/?St double terminal Chromosomal Translocation line. Line K15–1194-2 was identified as harboring a pair of 7DS/?StL Robertsonian Translocations and one 3D/?St double terminal Translocational chromosome. Further analyses using specific expressed sequence tag-SSR markers confirmed that the wheat–E. repens Translocations involved the 3St chromatin in both lines. Furthermore, compared with the wheat parent Chuannong16, K15–1192-2 and K15–1194-2 expressed high levels of resistance to FHB and stripe rust pathogens prevalent in China. Thus, this study has determined that the chromosome 3St of E. repens harbors gene(s) highly resistant to FHB and stripe rust, and chromatin of 3St introgressed into wheat chromosomes completely presented the resistance, indicating the feasibility of using these Translocation lines as novel material for breeding resistant wheat cultivars and alien gene mining.

Biran Gong - One of the best experts on this subject based on the ideXlab platform.

  • Molecular cytogenetic characterization of wheat–Elymus repens Chromosomal Translocation lines with resistance to Fusarium head blight and stripe rust
    BMC plant biology, 2019
    Co-Authors: Biran Gong, J. Zeng, Wei Zhu, Li Sanyue, Yuqi Wang, Yi Wang, Xing Fan, Lina Sha, Hai-qin Zhang
    Abstract:

    Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe and stripe rust caused by Puccinia striiformis f. sp. tritici are devastating diseases that affect wheat production worldwide. The use of disease-resistant genes and cultivars is the most effective means of reducing fungicide applications to combat these diseases. Elymus repens (2n = 6x = 42, StStStStHH) is a potentially useful germplasm of FHB and stripe rust resistance for wheat improvement. Here, we report the development and characterization of two wheat–E. repens lines derived from the progeny of common wheat–E. repens hybrids. Cytological studies indicated that the mean chromosome configuration of K15–1192-2 and K15–1194-2 at meiosis were 2n = 42 = 0.86 I + 17.46 II (ring) + 3.11 II (rod) and 2n = 42 = 2.45 I + 14.17 II (ring) + 5.50 II (rod) + 0.07 III, respectively. Genomic and fluorescence in situ hybridization karyotyping and simple sequence repeats markers revealed that K15–1192-2 was a wheat–E. repens 3D/?St double terminal Chromosomal Translocation line. Line K15–1194-2 was identified as harboring a pair of 7DS/?StL Robertsonian Translocations and one 3D/?St double terminal Translocational chromosome. Further analyses using specific expressed sequence tag-SSR markers confirmed that the wheat–E. repens Translocations involved the 3St chromatin in both lines. Furthermore, compared with the wheat parent Chuannong16, K15–1192-2 and K15–1194-2 expressed high levels of resistance to FHB and stripe rust pathogens prevalent in China. Thus, this study has determined that the chromosome 3St of E. repens harbors gene(s) highly resistant to FHB and stripe rust, and chromatin of 3St introgressed into wheat chromosomes completely presented the resistance, indicating the feasibility of using these Translocation lines as novel material for breeding resistant wheat cultivars and alien gene mining.

  • molecular cytogenetic characterization of wheat elymus repens Chromosomal Translocation lines with resistance to fusarium head blight and stripe rust
    BMC Plant Biology, 2019
    Co-Authors: Biran Gong, J. Zeng, Wei Zhu, Yuqi Wang, Yi Wang, Xing Fan, Lina Sha, Hai-qin Zhang, Lin Huang, Guoyue Chen
    Abstract:

    Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe and stripe rust caused by Puccinia striiformis f. sp. tritici are devastating diseases that affect wheat production worldwide. The use of disease-resistant genes and cultivars is the most effective means of reducing fungicide applications to combat these diseases. Elymus repens (2n = 6x = 42, StStStStHH) is a potentially useful germplasm of FHB and stripe rust resistance for wheat improvement. Here, we report the development and characterization of two wheat–E. repens lines derived from the progeny of common wheat–E. repens hybrids. Cytological studies indicated that the mean chromosome configuration of K15–1192-2 and K15–1194-2 at meiosis were 2n = 42 = 0.86 I + 17.46 II (ring) + 3.11 II (rod) and 2n = 42 = 2.45 I + 14.17 II (ring) + 5.50 II (rod) + 0.07 III, respectively. Genomic and fluorescence in situ hybridization karyotyping and simple sequence repeats markers revealed that K15–1192-2 was a wheat–E. repens 3D/?St double terminal Chromosomal Translocation line. Line K15–1194-2 was identified as harboring a pair of 7DS/?StL Robertsonian Translocations and one 3D/?St double terminal Translocational chromosome. Further analyses using specific expressed sequence tag-SSR markers confirmed that the wheat–E. repens Translocations involved the 3St chromatin in both lines. Furthermore, compared with the wheat parent Chuannong16, K15–1192-2 and K15–1194-2 expressed high levels of resistance to FHB and stripe rust pathogens prevalent in China. Thus, this study has determined that the chromosome 3St of E. repens harbors gene(s) highly resistant to FHB and stripe rust, and chromatin of 3St introgressed into wheat chromosomes completely presented the resistance, indicating the feasibility of using these Translocation lines as novel material for breeding resistant wheat cultivars and alien gene mining.

Choon Ki Lee - One of the best experts on this subject based on the ideXlab platform.

  • Skeletal overgrowth syndrome caused by overexpression of C-type natriuretic peptide in a girl with balanced Chromosomal Translocation, t(1;2)(q41;q37.1).
    American Journal of Medical Genetics Part A, 2015
    Co-Authors: Jun Seok Bae, Kohji Miura, Ok-hwa Kim, Keiichi Ozono, Jin Sun Choi, Hye Ran Lee, Na-young Kim, Choon Ki Lee
    Abstract:

    Chromosomal Translocation of 2q37.1 just distal to the NPPC gene coding for C-type natriuretic peptide (CNP) and subsequent overproduction of CNP have been reported to cause a skeletal overgrowth syndrome. Loeys–Dietz syndrome (LDS) is one of marfanoid overgrowth syndromes, of which subtype IV is caused by haploinsufficiency of transforming growth factor beta 2 (TGFB2). We report on a girl with clinical phenotypes of overgrowth syndrome, including long and slim body habitus, macrodactyly of the big toe, scoliosis, ankle valgus deformity, coxa valga, slipped capital femoral epiphysis, and aortic root dilatation. Karyotyping revealed a balanced Chromosomal Translocation between 1q41 and 2q37.1, and the breakpoints could be mapped by targeted resequencing analysis. On chromosome 2q37.1, the Translocation took place 200,365 bp downstream of NPPC, and serum level of the amino terminal of CNP was elevated. The contralateral site of Translocation on chromosome 1q41 disrupted TGFB2 gene, presumed to cause its haploinsufficiency. This case supports the concept that NPPC is overexpressed because of the loss of a specific negative regulatory control in the normal Chromosomal location, and demonstrates the effectiveness of targeted resequencing in the mapping of breakpoints. © 2015 Wiley Periodicals, Inc.

  • Skeletal overgrowth syndrome caused by overexpression of C-type natriuretic peptide in a girl with balanced Chromosomal Translocation, t(1;2)(q41;q37.1).
    American journal of medical genetics. Part A, 2015
    Co-Authors: Jun Seok Bae, Kohji Miura, Ok-hwa Kim, Keiichi Ozono, Jin Sun Choi, Hye Ran Lee, Nayoung K D Kim, Choon Ki Lee
    Abstract:

    Chromosomal Translocation of 2q37.1 just distal to the NPPC gene coding for C-type natriuretic peptide (CNP) and subsequent overproduction of CNP have been reported to cause a skeletal overgrowth syndrome. Loeys-Dietz syndrome (LDS) is one of marfanoid overgrowth syndromes, of which subtype IV is caused by haploinsufficiency of transforming growth factor beta 2 (TGFB2). We report on a girl with clinical phenotypes of overgrowth syndrome, including long and slim body habitus, macrodactyly of the big toe, scoliosis, ankle valgus deformity, coxa valga, slipped capital femoral epiphysis, and aortic root dilatation. Karyotyping revealed a balanced Chromosomal Translocation between 1q41 and 2q37.1, and the breakpoints could be mapped by targeted resequencing analysis. On chromosome 2q37.1, the Translocation took place 200,365 bp downstream of NPPC, and serum level of the amino terminal of CNP was elevated. The contralateral site of Translocation on chromosome 1q41 disrupted TGFB2 gene, presumed to cause its haploinsufficiency. This case supports the concept that NPPC is overexpressed because of the loss of a specific negative regulatory control in the normal Chromosomal location, and demonstrates the effectiveness of targeted resequencing in the mapping of breakpoints.

Guoyue Chen - One of the best experts on this subject based on the ideXlab platform.

  • molecular cytogenetic characterization of wheat elymus repens Chromosomal Translocation lines with resistance to fusarium head blight and stripe rust
    BMC Plant Biology, 2019
    Co-Authors: Biran Gong, J. Zeng, Wei Zhu, Yuqi Wang, Yi Wang, Xing Fan, Lina Sha, Hai-qin Zhang, Lin Huang, Guoyue Chen
    Abstract:

    Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe and stripe rust caused by Puccinia striiformis f. sp. tritici are devastating diseases that affect wheat production worldwide. The use of disease-resistant genes and cultivars is the most effective means of reducing fungicide applications to combat these diseases. Elymus repens (2n = 6x = 42, StStStStHH) is a potentially useful germplasm of FHB and stripe rust resistance for wheat improvement. Here, we report the development and characterization of two wheat–E. repens lines derived from the progeny of common wheat–E. repens hybrids. Cytological studies indicated that the mean chromosome configuration of K15–1192-2 and K15–1194-2 at meiosis were 2n = 42 = 0.86 I + 17.46 II (ring) + 3.11 II (rod) and 2n = 42 = 2.45 I + 14.17 II (ring) + 5.50 II (rod) + 0.07 III, respectively. Genomic and fluorescence in situ hybridization karyotyping and simple sequence repeats markers revealed that K15–1192-2 was a wheat–E. repens 3D/?St double terminal Chromosomal Translocation line. Line K15–1194-2 was identified as harboring a pair of 7DS/?StL Robertsonian Translocations and one 3D/?St double terminal Translocational chromosome. Further analyses using specific expressed sequence tag-SSR markers confirmed that the wheat–E. repens Translocations involved the 3St chromatin in both lines. Furthermore, compared with the wheat parent Chuannong16, K15–1192-2 and K15–1194-2 expressed high levels of resistance to FHB and stripe rust pathogens prevalent in China. Thus, this study has determined that the chromosome 3St of E. repens harbors gene(s) highly resistant to FHB and stripe rust, and chromatin of 3St introgressed into wheat chromosomes completely presented the resistance, indicating the feasibility of using these Translocation lines as novel material for breeding resistant wheat cultivars and alien gene mining.

Yi Wang - One of the best experts on this subject based on the ideXlab platform.

  • Molecular cytogenetic characterization of wheat–Elymus repens Chromosomal Translocation lines with resistance to Fusarium head blight and stripe rust
    BMC plant biology, 2019
    Co-Authors: Biran Gong, J. Zeng, Wei Zhu, Li Sanyue, Yuqi Wang, Yi Wang, Xing Fan, Lina Sha, Hai-qin Zhang
    Abstract:

    Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe and stripe rust caused by Puccinia striiformis f. sp. tritici are devastating diseases that affect wheat production worldwide. The use of disease-resistant genes and cultivars is the most effective means of reducing fungicide applications to combat these diseases. Elymus repens (2n = 6x = 42, StStStStHH) is a potentially useful germplasm of FHB and stripe rust resistance for wheat improvement. Here, we report the development and characterization of two wheat–E. repens lines derived from the progeny of common wheat–E. repens hybrids. Cytological studies indicated that the mean chromosome configuration of K15–1192-2 and K15–1194-2 at meiosis were 2n = 42 = 0.86 I + 17.46 II (ring) + 3.11 II (rod) and 2n = 42 = 2.45 I + 14.17 II (ring) + 5.50 II (rod) + 0.07 III, respectively. Genomic and fluorescence in situ hybridization karyotyping and simple sequence repeats markers revealed that K15–1192-2 was a wheat–E. repens 3D/?St double terminal Chromosomal Translocation line. Line K15–1194-2 was identified as harboring a pair of 7DS/?StL Robertsonian Translocations and one 3D/?St double terminal Translocational chromosome. Further analyses using specific expressed sequence tag-SSR markers confirmed that the wheat–E. repens Translocations involved the 3St chromatin in both lines. Furthermore, compared with the wheat parent Chuannong16, K15–1192-2 and K15–1194-2 expressed high levels of resistance to FHB and stripe rust pathogens prevalent in China. Thus, this study has determined that the chromosome 3St of E. repens harbors gene(s) highly resistant to FHB and stripe rust, and chromatin of 3St introgressed into wheat chromosomes completely presented the resistance, indicating the feasibility of using these Translocation lines as novel material for breeding resistant wheat cultivars and alien gene mining.

  • molecular cytogenetic characterization of wheat elymus repens Chromosomal Translocation lines with resistance to fusarium head blight and stripe rust
    BMC Plant Biology, 2019
    Co-Authors: Biran Gong, J. Zeng, Wei Zhu, Yuqi Wang, Yi Wang, Xing Fan, Lina Sha, Hai-qin Zhang, Lin Huang, Guoyue Chen
    Abstract:

    Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe and stripe rust caused by Puccinia striiformis f. sp. tritici are devastating diseases that affect wheat production worldwide. The use of disease-resistant genes and cultivars is the most effective means of reducing fungicide applications to combat these diseases. Elymus repens (2n = 6x = 42, StStStStHH) is a potentially useful germplasm of FHB and stripe rust resistance for wheat improvement. Here, we report the development and characterization of two wheat–E. repens lines derived from the progeny of common wheat–E. repens hybrids. Cytological studies indicated that the mean chromosome configuration of K15–1192-2 and K15–1194-2 at meiosis were 2n = 42 = 0.86 I + 17.46 II (ring) + 3.11 II (rod) and 2n = 42 = 2.45 I + 14.17 II (ring) + 5.50 II (rod) + 0.07 III, respectively. Genomic and fluorescence in situ hybridization karyotyping and simple sequence repeats markers revealed that K15–1192-2 was a wheat–E. repens 3D/?St double terminal Chromosomal Translocation line. Line K15–1194-2 was identified as harboring a pair of 7DS/?StL Robertsonian Translocations and one 3D/?St double terminal Translocational chromosome. Further analyses using specific expressed sequence tag-SSR markers confirmed that the wheat–E. repens Translocations involved the 3St chromatin in both lines. Furthermore, compared with the wheat parent Chuannong16, K15–1192-2 and K15–1194-2 expressed high levels of resistance to FHB and stripe rust pathogens prevalent in China. Thus, this study has determined that the chromosome 3St of E. repens harbors gene(s) highly resistant to FHB and stripe rust, and chromatin of 3St introgressed into wheat chromosomes completely presented the resistance, indicating the feasibility of using these Translocation lines as novel material for breeding resistant wheat cultivars and alien gene mining.