The Experts below are selected from a list of 12 Experts worldwide ranked by ideXlab platform

Danny R Welch - One of the best experts on this subject based on the ideXlab platform.

  • identification of highly expressed genes in metastasis suppressed Chromosome 6 Human malignant melanoma hybrid cells using subtractive hybridization and differential display
    International Journal of Cancer, 1997
    Co-Authors: Jeonghyung Lee, Danny R Welch
    Abstract:

    Microcell-mediated transfer of Chromosome 6 into Human melanoma cell lines C8161 and MelJuSo suppresses metastasis by at least 95% without affecting tumorigenicity. Subtractive hybridization and differential display were used to identify the molecule(s) responsible for suppressing metastasis in neo6/melanoma (neo6/C8161 and neo6/MelJuSo) hybrids. Seven cDNA clones exhibiting quantitatively or qualitatively higher expression in neo6/melanoma hybrids were obtained. These genes fell into 2 categories: 1) transcription-related genes (AP-2A, HMG-I(Y) and a novel isoform of nucleophosmin B23), which have previously been shown to regulate metastasis-associated genes; and 2) novel genes. One of the novel genes, designated KiSS-1, significantly suppressed metastasis of the Human malignant melanoma cell lines MelJuSo and a highly metastatic subclone of C8161, C8161cl.9, following transfection and constitutive expression. Our results illustrate the power of subtractive hybridization and differential display to identify functional metastasis-controlling genes in Human melanoma.Int. J. Cancer 71: 1035-1044, 1997. © 1997 Wiley-Liss Inc.

Jeonghyung Lee - One of the best experts on this subject based on the ideXlab platform.

  • identification of highly expressed genes in metastasis suppressed Chromosome 6 Human malignant melanoma hybrid cells using subtractive hybridization and differential display
    International Journal of Cancer, 1997
    Co-Authors: Jeonghyung Lee, Danny R Welch
    Abstract:

    Microcell-mediated transfer of Chromosome 6 into Human melanoma cell lines C8161 and MelJuSo suppresses metastasis by at least 95% without affecting tumorigenicity. Subtractive hybridization and differential display were used to identify the molecule(s) responsible for suppressing metastasis in neo6/melanoma (neo6/C8161 and neo6/MelJuSo) hybrids. Seven cDNA clones exhibiting quantitatively or qualitatively higher expression in neo6/melanoma hybrids were obtained. These genes fell into 2 categories: 1) transcription-related genes (AP-2A, HMG-I(Y) and a novel isoform of nucleophosmin B23), which have previously been shown to regulate metastasis-associated genes; and 2) novel genes. One of the novel genes, designated KiSS-1, significantly suppressed metastasis of the Human malignant melanoma cell lines MelJuSo and a highly metastatic subclone of C8161, C8161cl.9, following transfection and constitutive expression. Our results illustrate the power of subtractive hybridization and differential display to identify functional metastasis-controlling genes in Human melanoma.Int. J. Cancer 71: 1035-1044, 1997. © 1997 Wiley-Liss Inc.

Manfred Schwerin - One of the best experts on this subject based on the ideXlab platform.

  • targeted construction of a high resolution integrated comprehensive and comparative map for a region specific to bovine Chromosome 6 based on radiation hybrid mapping
    Genomics, 2002
    Co-Authors: Rosemarie Weikard, Christa Kuhn, Tom Goldammer, P Laurent, J E Womack, Manfred Schwerin
    Abstract:

    Abstract To resolve a candidate Chromosome region on the middle part of bovine Chromosome 6 (BTA6) containing several different quantitative trait locus (QTL) intervals, we constructed a high-resolution, integrated, comprehensive, and comparative map using a 12,000-rad, wholegenome, cattle–hamster radiation hybrid (RH) panel. The RH map includes a total of 71 loci either selected from bovine and comparative maps or targeted directly from a microdissection library specific for the BTA6 region. All loci typed were placed in one linkage group at a lod score threshold of 4.0. The length of the comprehensive RH map, which is the first highresolution RH map in cattle, spans 2568.8 cR12,000. The order of markers obtained principally agrees with the order on published bovine genetic maps. Our RH map integrates markers as well as genes and ESTs available from several physical and genetic maps of BTA6 and the orthologous ovine Chromosome 6, Human Chromosome 4, and mouse Chromosomes 5/3. Comparative analysis confirms and refines current knowledge about conservation and rearrangements in corresponding chromosomal regions on BTA6. We identified and localized two new breakpoints for intrachromosomal rearrangements between Human Chromosome 4 and BTA6. This RH map is a powerful tool in all aspects of genetic, physical, transcript, and comparative mapping. Due to its links to the gene-dense maps of Human and mouse, it can serve as a prerequisite to identify possible candidate genes for quantitative trait loci localized in the targeted BTA6 region.

Rosemarie Weikard - One of the best experts on this subject based on the ideXlab platform.

  • targeted construction of a high resolution integrated comprehensive and comparative map for a region specific to bovine Chromosome 6 based on radiation hybrid mapping
    Genomics, 2002
    Co-Authors: Rosemarie Weikard, Christa Kuhn, Tom Goldammer, P Laurent, J E Womack, Manfred Schwerin
    Abstract:

    Abstract To resolve a candidate Chromosome region on the middle part of bovine Chromosome 6 (BTA6) containing several different quantitative trait locus (QTL) intervals, we constructed a high-resolution, integrated, comprehensive, and comparative map using a 12,000-rad, wholegenome, cattle–hamster radiation hybrid (RH) panel. The RH map includes a total of 71 loci either selected from bovine and comparative maps or targeted directly from a microdissection library specific for the BTA6 region. All loci typed were placed in one linkage group at a lod score threshold of 4.0. The length of the comprehensive RH map, which is the first highresolution RH map in cattle, spans 2568.8 cR12,000. The order of markers obtained principally agrees with the order on published bovine genetic maps. Our RH map integrates markers as well as genes and ESTs available from several physical and genetic maps of BTA6 and the orthologous ovine Chromosome 6, Human Chromosome 4, and mouse Chromosomes 5/3. Comparative analysis confirms and refines current knowledge about conservation and rearrangements in corresponding chromosomal regions on BTA6. We identified and localized two new breakpoints for intrachromosomal rearrangements between Human Chromosome 4 and BTA6. This RH map is a powerful tool in all aspects of genetic, physical, transcript, and comparative mapping. Due to its links to the gene-dense maps of Human and mouse, it can serve as a prerequisite to identify possible candidate genes for quantitative trait loci localized in the targeted BTA6 region.

J E Womack - One of the best experts on this subject based on the ideXlab platform.

  • targeted construction of a high resolution integrated comprehensive and comparative map for a region specific to bovine Chromosome 6 based on radiation hybrid mapping
    Genomics, 2002
    Co-Authors: Rosemarie Weikard, Christa Kuhn, Tom Goldammer, P Laurent, J E Womack, Manfred Schwerin
    Abstract:

    Abstract To resolve a candidate Chromosome region on the middle part of bovine Chromosome 6 (BTA6) containing several different quantitative trait locus (QTL) intervals, we constructed a high-resolution, integrated, comprehensive, and comparative map using a 12,000-rad, wholegenome, cattle–hamster radiation hybrid (RH) panel. The RH map includes a total of 71 loci either selected from bovine and comparative maps or targeted directly from a microdissection library specific for the BTA6 region. All loci typed were placed in one linkage group at a lod score threshold of 4.0. The length of the comprehensive RH map, which is the first highresolution RH map in cattle, spans 2568.8 cR12,000. The order of markers obtained principally agrees with the order on published bovine genetic maps. Our RH map integrates markers as well as genes and ESTs available from several physical and genetic maps of BTA6 and the orthologous ovine Chromosome 6, Human Chromosome 4, and mouse Chromosomes 5/3. Comparative analysis confirms and refines current knowledge about conservation and rearrangements in corresponding chromosomal regions on BTA6. We identified and localized two new breakpoints for intrachromosomal rearrangements between Human Chromosome 4 and BTA6. This RH map is a powerful tool in all aspects of genetic, physical, transcript, and comparative mapping. Due to its links to the gene-dense maps of Human and mouse, it can serve as a prerequisite to identify possible candidate genes for quantitative trait loci localized in the targeted BTA6 region.