The Experts below are selected from a list of 9 Experts worldwide ranked by ideXlab platform
A.t. Natarajan - One of the best experts on this subject based on the ideXlab platform.
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Discrimination between complete and incomplete Chromosome exchanges in X-irradiated Human lymphocytes using FISH with pan-centromeric and Chromosome specific DNA probes in combination with telomeric PNA probe.
International journal of radiation biology, 2000Co-Authors: J. Fomina, Firouz Darroudi, J. J. W. A. Boei, A.t. NatarajanAbstract:Purpose : To discriminate precisely between radiation-induced complete and incomplete Chromosome exchanges using Chromosome painting together with the detection of the centromeres and telomeres in one FISH assay. Materials and methods : Human lymphocytes were exposed in vitro to X-rays at a dose of 4 Gy. Chromosome aberrations were analysed using the FISH technique in combination with a whole Chromosome-specific DNA probe for Chromosome 8, Human pan-centromeric DNA and telomeric PNA probes. Results : The combined FISH assay has improved the resolution of detecting chromosomal exchanges in Human lymphocytes. Results indicate that the frequency of observed incomplete exchange patterns was 21% when telomeric signals were ignored during the analysis. When the telomeric signals were included in the analysis a large proportion of apparently incomplete exchange patterns appeared complete and should be re-classified. The percentage of true incomplete exchanges was found to be less than 5%. Conclusion : The combin...
J. Fomina - One of the best experts on this subject based on the ideXlab platform.
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Discrimination between complete and incomplete Chromosome exchanges in X-irradiated Human lymphocytes using FISH with pan-centromeric and Chromosome specific DNA probes in combination with telomeric PNA probe.
International journal of radiation biology, 2000Co-Authors: J. Fomina, Firouz Darroudi, J. J. W. A. Boei, A.t. NatarajanAbstract:Purpose : To discriminate precisely between radiation-induced complete and incomplete Chromosome exchanges using Chromosome painting together with the detection of the centromeres and telomeres in one FISH assay. Materials and methods : Human lymphocytes were exposed in vitro to X-rays at a dose of 4 Gy. Chromosome aberrations were analysed using the FISH technique in combination with a whole Chromosome-specific DNA probe for Chromosome 8, Human pan-centromeric DNA and telomeric PNA probes. Results : The combined FISH assay has improved the resolution of detecting chromosomal exchanges in Human lymphocytes. Results indicate that the frequency of observed incomplete exchange patterns was 21% when telomeric signals were ignored during the analysis. When the telomeric signals were included in the analysis a large proportion of apparently incomplete exchange patterns appeared complete and should be re-classified. The percentage of true incomplete exchanges was found to be less than 5%. Conclusion : The combin...
Firouz Darroudi - One of the best experts on this subject based on the ideXlab platform.
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Discrimination between complete and incomplete Chromosome exchanges in X-irradiated Human lymphocytes using FISH with pan-centromeric and Chromosome specific DNA probes in combination with telomeric PNA probe.
International journal of radiation biology, 2000Co-Authors: J. Fomina, Firouz Darroudi, J. J. W. A. Boei, A.t. NatarajanAbstract:Purpose : To discriminate precisely between radiation-induced complete and incomplete Chromosome exchanges using Chromosome painting together with the detection of the centromeres and telomeres in one FISH assay. Materials and methods : Human lymphocytes were exposed in vitro to X-rays at a dose of 4 Gy. Chromosome aberrations were analysed using the FISH technique in combination with a whole Chromosome-specific DNA probe for Chromosome 8, Human pan-centromeric DNA and telomeric PNA probes. Results : The combined FISH assay has improved the resolution of detecting chromosomal exchanges in Human lymphocytes. Results indicate that the frequency of observed incomplete exchange patterns was 21% when telomeric signals were ignored during the analysis. When the telomeric signals were included in the analysis a large proportion of apparently incomplete exchange patterns appeared complete and should be re-classified. The percentage of true incomplete exchanges was found to be less than 5%. Conclusion : The combin...
J. J. W. A. Boei - One of the best experts on this subject based on the ideXlab platform.
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Discrimination between complete and incomplete Chromosome exchanges in X-irradiated Human lymphocytes using FISH with pan-centromeric and Chromosome specific DNA probes in combination with telomeric PNA probe.
International journal of radiation biology, 2000Co-Authors: J. Fomina, Firouz Darroudi, J. J. W. A. Boei, A.t. NatarajanAbstract:Purpose : To discriminate precisely between radiation-induced complete and incomplete Chromosome exchanges using Chromosome painting together with the detection of the centromeres and telomeres in one FISH assay. Materials and methods : Human lymphocytes were exposed in vitro to X-rays at a dose of 4 Gy. Chromosome aberrations were analysed using the FISH technique in combination with a whole Chromosome-specific DNA probe for Chromosome 8, Human pan-centromeric DNA and telomeric PNA probes. Results : The combined FISH assay has improved the resolution of detecting chromosomal exchanges in Human lymphocytes. Results indicate that the frequency of observed incomplete exchange patterns was 21% when telomeric signals were ignored during the analysis. When the telomeric signals were included in the analysis a large proportion of apparently incomplete exchange patterns appeared complete and should be re-classified. The percentage of true incomplete exchanges was found to be less than 5%. Conclusion : The combin...
Ian Tomlinson - One of the best experts on this subject based on the ideXlab platform.
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The Utility of Mouse Models in Post-GWAS Research
Science (New York N.Y.), 2012Co-Authors: Annabelle Lewis, Ian TomlinsonAbstract:Genome-wide association studies (GWAS) have discovered over 100 haplotype-tagging single-nucleotide polymorphisms (SNPs) that are associated with cancer risk. The small region between 128 and 129 Mb on the long arm of Human Chromosome 8 (Human Genome Build 37) notably contains a number of uncorrelated cancer predisposition SNPs (see the figure) ( 1 – 6 ). There are probably five or more different causal genetic variants in the region, and each is independently associated with the risk of one or more common cancers. Perhaps the most promiscuous variant is rs6983267—a common SNP in which guanine (G) is replaced by thymine (T)—at which the G allele is associated with increased risk of cancers of the prostate, large bowel, and thyroid. On page 1360 in this issue, Sur et al. ( 7 ) use a loss-of-function animal model to confirm that the region around the noncoding rs6983267 SNP identified using Human GWAS probably has direct functional effects on tumorigenesis.