The Experts below are selected from a list of 12 Experts worldwide ranked by ideXlab platform
Michael Bender - One of the best experts on this subject based on the ideXlab platform.
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dhr3 an ecdysone inducible early late gene encoding a drosophila nuclear receptor is required for embryogenesis
Proceedings of the National Academy of Sciences of the United States of America, 1997Co-Authors: Ginger E Carney, Andrew A Wade, Rajat Sapra, Elliott S Goldstein, Michael BenderAbstract:Response to the steroid hormone ecdysone in Drosophila is controlled by genetic regulatory hierarchies that include eight members of the nuclear receptor protein family. The DHR3 gene, located within the 46F early-late ecdysone-inducible Chromosome Puff, encodes an orphan nuclear receptor that recently has been shown to exert both positive and negative regulatory effects in the ecdysone-induced genetic hierarchies at metamorphosis. We used a reverse genetics approach to identify 11 DHR3 mutants from a pool of lethal mutations in the 46F region on the second Chromosome. Two DHR3 mutations result in amino acid substitutions within the conserved DNA binding domain. Analysis of DHR3 mutants reveals that DHR3 function is required to complete embryogenesis. All DHR3 alleles examined result in nervous system defects in the embryo.
Pamela K Geyer - One of the best experts on this subject based on the ideXlab platform.
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studies of the role of the drosophila scs and scs insulators in defining boundaries of a Chromosome Puff
Molecular and Cellular Biology, 2004Co-Authors: Emily J Kuhn, Craig M Hart, Pamela K GeyerAbstract:Insulators are DNA elements that establish independent transcriptional domains within eukaryotic genomes. The Drosophila scs and scs′ insulators localize near the borders of a structural domain in the polytene Chromosomes, known as a Puff, produced by transcription of the 87A heat shock protein (hsp) genes. It has been suggested that scs and scs′ are boundary elements that delimit this decondensed chromatin domain, reflecting the mechanism by which these sequences act to constrain regulatory interactions. This model was tested using transposons that carried a yellow gene to assess enhancer blocking and an hsp70-lacZ gene to examine the structure of a heat shock Puff in the presence and absence of insulators. We found that although scs and scs′ blocked enhancer function, these sequences did not prevent the spread of decondensation resulting from hsp70-lacZ transcription. Further analysis of the endogenous 87A locus demonstrated that scs and scs′ reside within, not at, the borders of the Puff. Taken together, our studies suggest that scs and scs′ are not boundary elements that block the propagation of an altered chromatin state associated with Puff formation. We propose that these insulators may have a direct role in limiting regulatory interactions in the gene-dense 87A region.
Ginger E Carney - One of the best experts on this subject based on the ideXlab platform.
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dhr3 an ecdysone inducible early late gene encoding a drosophila nuclear receptor is required for embryogenesis
Proceedings of the National Academy of Sciences of the United States of America, 1997Co-Authors: Ginger E Carney, Andrew A Wade, Rajat Sapra, Elliott S Goldstein, Michael BenderAbstract:Response to the steroid hormone ecdysone in Drosophila is controlled by genetic regulatory hierarchies that include eight members of the nuclear receptor protein family. The DHR3 gene, located within the 46F early-late ecdysone-inducible Chromosome Puff, encodes an orphan nuclear receptor that recently has been shown to exert both positive and negative regulatory effects in the ecdysone-induced genetic hierarchies at metamorphosis. We used a reverse genetics approach to identify 11 DHR3 mutants from a pool of lethal mutations in the 46F region on the second Chromosome. Two DHR3 mutations result in amino acid substitutions within the conserved DNA binding domain. Analysis of DHR3 mutants reveals that DHR3 function is required to complete embryogenesis. All DHR3 alleles examined result in nervous system defects in the embryo.
Emily J Kuhn - One of the best experts on this subject based on the ideXlab platform.
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studies of the role of the drosophila scs and scs insulators in defining boundaries of a Chromosome Puff
Molecular and Cellular Biology, 2004Co-Authors: Emily J Kuhn, Craig M Hart, Pamela K GeyerAbstract:Insulators are DNA elements that establish independent transcriptional domains within eukaryotic genomes. The Drosophila scs and scs′ insulators localize near the borders of a structural domain in the polytene Chromosomes, known as a Puff, produced by transcription of the 87A heat shock protein (hsp) genes. It has been suggested that scs and scs′ are boundary elements that delimit this decondensed chromatin domain, reflecting the mechanism by which these sequences act to constrain regulatory interactions. This model was tested using transposons that carried a yellow gene to assess enhancer blocking and an hsp70-lacZ gene to examine the structure of a heat shock Puff in the presence and absence of insulators. We found that although scs and scs′ blocked enhancer function, these sequences did not prevent the spread of decondensation resulting from hsp70-lacZ transcription. Further analysis of the endogenous 87A locus demonstrated that scs and scs′ reside within, not at, the borders of the Puff. Taken together, our studies suggest that scs and scs′ are not boundary elements that block the propagation of an altered chromatin state associated with Puff formation. We propose that these insulators may have a direct role in limiting regulatory interactions in the gene-dense 87A region.
Elliott S Goldstein - One of the best experts on this subject based on the ideXlab platform.
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dhr3 an ecdysone inducible early late gene encoding a drosophila nuclear receptor is required for embryogenesis
Proceedings of the National Academy of Sciences of the United States of America, 1997Co-Authors: Ginger E Carney, Andrew A Wade, Rajat Sapra, Elliott S Goldstein, Michael BenderAbstract:Response to the steroid hormone ecdysone in Drosophila is controlled by genetic regulatory hierarchies that include eight members of the nuclear receptor protein family. The DHR3 gene, located within the 46F early-late ecdysone-inducible Chromosome Puff, encodes an orphan nuclear receptor that recently has been shown to exert both positive and negative regulatory effects in the ecdysone-induced genetic hierarchies at metamorphosis. We used a reverse genetics approach to identify 11 DHR3 mutants from a pool of lethal mutations in the 46F region on the second Chromosome. Two DHR3 mutations result in amino acid substitutions within the conserved DNA binding domain. Analysis of DHR3 mutants reveals that DHR3 function is required to complete embryogenesis. All DHR3 alleles examined result in nervous system defects in the embryo.