The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform
Benoit Kammerer - One of the best experts on this subject based on the ideXlab platform.
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determination of Chromosome Size and number of rrn loci in lactobacillus plantarum by pulsed field gel electrophoresis
Fems Microbiology Letters, 1994Co-Authors: Bruno Chevallier, Jean Hubert, Benoit KammererAbstract:The Size of the Lactobacillus plantarum CCM 1904 Chromosome was determined by pulse-field gel electrophoresis. It was found to be 3.3–3.4 Mb using SfiI or AscI restriction endonucleases, compared to 3–4 Mb found for the other L. plantarum strains tested. L. plantarum CCM 1904 5S rDNA was cloned by polymerase chain reaction, sequenced, and used as a probe to characterize strains. At least five rrn loci were found. The pulsed-field gel electrophoresis macrorestriction patterns were strain-specific, while the rDNA restriction hybridization patterns were species-specific.
Jacque Lamb - One of the best experts on this subject based on the ideXlab platform.
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Chromosome Size dependent control of meiotic reciprocal recombination in saccharomyces cerevisiae the role of crossover interference
Genetics, 1999Co-Authors: David B Kaback, Dianna Barber, Jim Mahon, Jacque LambAbstract:In the yeast Saccharomyces cerevisiae, small Chromosomes undergo meiotic reciprocal recombination (crossing over) at rates (centimorgans per kilobases) greater than those of large Chromosomes, and recombination rates respond directly to changes in the total Size of a chromosomal DNA molecule. This phenomenon, termed Chromosome Size-dependent control of meiotic reciprocal recombination, has been suggested to be important for ensuring that homologous Chromosomes cross over during meiosis. The mechanism of this regulation was investigated by analyzing recombination in identical genetic intervals present on different Size Chromosomes. The results indicate that Chromosome Size-dependent control is due to different amounts of crossover interference. Large Chromosomes have high levels of interference while small Chromosomes have much lower levels of interference. A model for how crossover interference directly responds to Chromosome Size is presented. In addition, Chromosome Size-dependent control was shown to lower the frequency of homologous Chromosomes that failed to undergo crossovers, suggesting that this control is an integral part of the mechanism for ensuring meiotic crossing over between homologous Chromosomes.
Karl Otto Greulich - One of the best experts on this subject based on the ideXlab platform.
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uv a breakage sensitivity of human Chromosomes as measured by comet fish depends on gene density and not on the Chromosome Size
Journal of Photochemistry and Photobiology B-biology, 2000Co-Authors: Alexander Rapp, Heike Dittmar, Claudia Bock, Karl Otto GreulichAbstract:COMET-FISH, a single cell-based combination of COMET-assay (also known as single cell gel electrophoresis (SCGE)) with fluorescence in situ hybridization (FISH) allows region specific studies on DNA stability and damage. COMET-FISH can be used to investigate UV-A-induced DNA damage of selected whole Chromosomes. In the present work, a modified COMET-FISH protocol with whole Chromosome painting probes was used to study whether UV-A-induced DNA damage is distributed randomly over the whole genome or occurs at preferred sites. The study was performed with 12 different Chromosome painting probes (for Chromosomes 1, 2, 3, 8, 9, 11, 14, 18, 19, 21, X and Y). The results on human lymphocytes irradiated with 500 kJ/m2 at a wavelength of 365 nm indicate that the induced number of chromatin strand breaks does not correlate with the Chromosome Size. They therefore are distributed in a non-random manner. For example, fragments of the gene-rich Chromosome Chromosome 1 were found in the comet tail in only 3% of the examined cells, and thus Chromosome 1 is rather stable, whereas fragmentation of the gene-poor Chromosome 8 was observed in 25% of all comets. On the basis of all 12 Chromosomes analyzed, an inverse correlation between the density of active genes and the sensitivity toward UV-A radiation is found.
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UV-A breakage sensitivity of human Chromosomes as measured by COMET-FISH depends on gene density and not on the Chromosome Size
Journal of Photochemistry and Photobiology B: Biology, 2000Co-Authors: Alexander Rapp, Heike Dittmar, Claudia Bock, Karl Otto GreulichAbstract:COMET-FISH, a single cell-based combination of COMET-assay (also known as single cell gel electrophoresis (SCGE)) with fluorescence in situ hybridization (FISH) allows region specific studies on DNA stability and damage. COMET-FISH can be used to investigate UV-A-induced DNA damage of selected whole Chromosomes. In the present work, a modified COMET-FISH protocol with whole Chromosome painting probes was used to study whether UV-A-induced DNA damage is distributed randomly over the whole genome or occurs at preferred sites. The study was performed with 12 different Chromosome painting probes (for Chromosomes 1, 2, 3, 8, 9, 11, 14, 18, 19, 21, X and Y). The results on human lymphocytes irradiated with 500 kJ/m2 at a wavelength of 365 nm indicate that the induced number of chromatin strand breaks does not correlate with the Chromosome Size. They therefore are distributed in a non-random manner. For example, fragments of the gene-rich Chromosome Chromosome 1 were found in the comet tail in only 3% of the examined cells, and thus Chromosome 1 is rather stable, whereas fragmentation of the gene-poor Chromosome 8 was observed in 25% of all comets. On the basis of all 12 Chromosomes analyzed, an inverse correlation between the density of active genes and the sensitivity toward UV-A radiation is found. (C) 2000 Elsevier Science S.A. All rights reserved.
David B Kaback - One of the best experts on this subject based on the ideXlab platform.
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Chromosome Size dependent control of meiotic reciprocal recombination in saccharomyces cerevisiae the role of crossover interference
Genetics, 1999Co-Authors: David B Kaback, Dianna Barber, Jim Mahon, Jacque LambAbstract:In the yeast Saccharomyces cerevisiae, small Chromosomes undergo meiotic reciprocal recombination (crossing over) at rates (centimorgans per kilobases) greater than those of large Chromosomes, and recombination rates respond directly to changes in the total Size of a chromosomal DNA molecule. This phenomenon, termed Chromosome Size-dependent control of meiotic reciprocal recombination, has been suggested to be important for ensuring that homologous Chromosomes cross over during meiosis. The mechanism of this regulation was investigated by analyzing recombination in identical genetic intervals present on different Size Chromosomes. The results indicate that Chromosome Size-dependent control is due to different amounts of crossover interference. Large Chromosomes have high levels of interference while small Chromosomes have much lower levels of interference. A model for how crossover interference directly responds to Chromosome Size is presented. In addition, Chromosome Size-dependent control was shown to lower the frequency of homologous Chromosomes that failed to undergo crossovers, suggesting that this control is an integral part of the mechanism for ensuring meiotic crossing over between homologous Chromosomes.
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Chromosome Size dependent control of meiotic recombination
Science, 1992Co-Authors: David B Kaback, V Guacci, Dianna Barber, J W MahonAbstract:Smaller Chromosomes have higher rates of meiotic reciprocal recombination (centimorgans per kilobase pair) than larger Chromosomes. This report demonstrates that decreasing the Size of Saccharomyces cerevisiae chromosomal DNA molecules increases rates of meiotic recombination and increasing Chromosome Size decreases recombination rates. These results indicate that Chromosome Size directly affects meiotic reciprocal recombination.
Bruno Chevallier - One of the best experts on this subject based on the ideXlab platform.
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determination of Chromosome Size and number of rrn loci in lactobacillus plantarum by pulsed field gel electrophoresis
Fems Microbiology Letters, 1994Co-Authors: Bruno Chevallier, Jean Hubert, Benoit KammererAbstract:The Size of the Lactobacillus plantarum CCM 1904 Chromosome was determined by pulse-field gel electrophoresis. It was found to be 3.3–3.4 Mb using SfiI or AscI restriction endonucleases, compared to 3–4 Mb found for the other L. plantarum strains tested. L. plantarum CCM 1904 5S rDNA was cloned by polymerase chain reaction, sequenced, and used as a probe to characterize strains. At least five rrn loci were found. The pulsed-field gel electrophoresis macrorestriction patterns were strain-specific, while the rDNA restriction hybridization patterns were species-specific.