The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform

Yue Jin - One of the best experts on this subject based on the ideXlab platform.

  • Molecular and cytogenetic characterization of a durum wheat–Aegilops speltoides Chromosome Translocation conferring resistance to stem rust
    Chromosome Research, 2008
    Co-Authors: Justin D. Faris, Xiwen Cai, Timothy L. Friesen, Yue Jin
    Abstract:

    Stem rust is a serious disease of wheat that has caused historical epidemics, but it has not been a threat in recent decades in North America owing to the eradication of the alternative host and deployment of resistant cultivars. However, the recent emergence of Ug99 (or race TTKS) poses a threat to global wheat production because most currently grown wheat varieties are susceptible. In this study, we evaluated a durum wheat– Aegilops speltoides Chromosome Translocation line (DAS15) for reaction to Ug99 and six other races of stem rust, and used molecular and cytogenetic tools to characterize the Translocation. DAS15 was resistant to all seven races of stem rust. Two durum– Ae. speltoides translocated Chromosomes were detected in DAS15. One Translocation involved the short arm, centromere, and a major portion of the long arm of Ae . speltoides Chromosome 2S and a small terminal segment from durum Chromosome arm 2BL. Thus, this translocated Chromosome is designated T2BL-2SL•2SS. Cytogenetic mapping assigned the resistance gene(s) in DAS15 to the Ae. speltoides segment in T2BL-2SL•2SS. The Ae. speltoides segment in the other translocated Chromosome did not harbour stem rust resistance. A comparison of DAS15 and the wheat stocks carrying the Ae. speltoides -derived resistance genes Sr32 and Sr39 indicated that stem rust resistance gene present in DAS15 is likely novel and will be useful for developing germplasm with resistance to Ug99. Efforts to reduce Ae. speltoides chromatin in T2BL-2SL•2SS are currently in progress.

  • molecular and cytogenetic characterization of a durum wheat aegilops speltoides Chromosome Translocation conferring resistance to stem rust
    Chromosome Research, 2008
    Co-Authors: Justin D. Faris, Xiwen Cai, Timothy L. Friesen, Yue Jin
    Abstract:

    Stem rust is a serious disease of wheat that has caused historical epidemics, but it has not been a threat in recent decades in North America owing to the eradication of the alternative host and deployment of resistant cultivars. However, the recent emergence of Ug99 (or race TTKS) poses a threat to global wheat production because most currently grown wheat varieties are susceptible. In this study, we evaluated a durum wheat–Aegilops speltoides Chromosome Translocation line (DAS15) for reaction to Ug99 and six other races of stem rust, and used molecular and cytogenetic tools to characterize the Translocation. DAS15 was resistant to all seven races of stem rust. Two durum–Ae. speltoides translocated Chromosomes were detected in DAS15. One Translocation involved the short arm, centromere, and a major portion of the long arm of Ae. speltoides Chromosome 2S and a small terminal segment from durum Chromosome arm 2BL. Thus, this translocated Chromosome is designated T2BL-2SL•2SS. Cytogenetic mapping assigned the resistance gene(s) in DAS15 to the Ae. speltoides segment in T2BL-2SL•2SS. The Ae. speltoides segment in the other translocated Chromosome did not harbour stem rust resistance. A comparison of DAS15 and the wheat stocks carrying the Ae. speltoides-derived resistance genes Sr32 and Sr39 indicated that stem rust resistance gene present in DAS15 is likely novel and will be useful for developing germplasm with resistance to Ug99. Efforts to reduce Ae. speltoides chromatin in T2BL-2SL•2SS are currently in progress.

Maciej Kurpisz - One of the best experts on this subject based on the ideXlab platform.

  • Chromosome re positioning in spermatozoa of fathers and sons carriers of reciprocal Chromosome Translocation rct
    BMC Medical Genomics, 2019
    Co-Authors: Marta Olszewska, Alina T. Midro, Ewa Wiland, Nataliya Huleyuk, Monika Fraczek, Danuta Zastavna, Maciej Kurpisz
    Abstract:

    Non-random Chromosome positioning has been observed in the nuclei of several different tissue types, including human spermatozoa. The nuclear arrangement of Chromosomes can be altered in men with decreased semen parameters or increased DNA fragmentation and in males with chromosomal numerical or structural aberrations. An aim of this study was to determine whether and how the positioning of nine Chromosome centromeres was (re)arranged in the spermatozoa of fathers and sons – carriers of the same reciprocal Chromosome Translocation (RCT). Fluorescence in situ hybridization (FISH) was applied to analyse the positioning of sperm Chromosomes in a group of 13 carriers of 11 RCTs, including two familial RCT cases: t(4;5) and t(7;10), followed by analysis of eight control individuals. Additionally, sperm chromatin integrity was evaluated using TUNEL and Aniline Blue techniques. In the analysed familial RCT cases, repositioning of the Chromosomes occurred in a similar way when compared to the data generated in healthy controls, even if some differences between father and son were further observed. These differences might have arisen from various statuses of sperm chromatin disintegration. Nuclear topology appears as another aspect of epigenetic genomic regulation that may influence DNA functioning. We have re-documented that chromosomal positioning is defined in control males and that a particular RCT is reflected in the individual pattern of chromosomal topology. The present study examining the collected RCT group, including two familial cases, additionally showed that chromosomal factors (karyotype and hyperhaploidy) have superior effects, strongly influencing the chromosomal topology, when confronted with sperm chromatin integrity components (DNA fragmentation or chromatin deprotamination).

  • Chromosome (re)positioning in spermatozoa of fathers and sons – carriers of reciprocal Chromosome Translocation (RCT)
    BMC Medical Genomics, 2019
    Co-Authors: Marta Olszewska, Alina T. Midro, Ewa Wiland, Nataliya Huleyuk, Monika Fraczek, Danuta Zastavna, Maciej Kurpisz
    Abstract:

    Non-random Chromosome positioning has been observed in the nuclei of several different tissue types, including human spermatozoa. The nuclear arrangement of Chromosomes can be altered in men with decreased semen parameters or increased DNA fragmentation and in males with chromosomal numerical or structural aberrations. An aim of this study was to determine whether and how the positioning of nine Chromosome centromeres was (re)arranged in the spermatozoa of fathers and sons – carriers of the same reciprocal Chromosome Translocation (RCT). Fluorescence in situ hybridization (FISH) was applied to analyse the positioning of sperm Chromosomes in a group of 13 carriers of 11 RCTs, including two familial RCT cases: t(4;5) and t(7;10), followed by analysis of eight control individuals. Additionally, sperm chromatin integrity was evaluated using TUNEL and Aniline Blue techniques. In the analysed familial RCT cases, repositioning of the Chromosomes occurred in a similar way when compared to the data generated in healthy controls, even if some differences between father and son were further observed. These differences might have arisen from various statuses of sperm chromatin disintegration. Nuclear topology appears as another aspect of epigenetic genomic regulation that may influence DNA functioning. We have re-documented that chromosomal positioning is defined in control males and that a particular RCT is reflected in the individual pattern of chromosomal topology. The present study examining the collected RCT group, including two familial cases, additionally showed that chromosomal factors (karyotype and hyperhaploidy) have superior effects, strongly influencing the chromosomal topology, when confronted with sperm chromatin integrity components (DNA fragmentation or chromatin deprotamination).

  • Chromosome (re)positioning in spermatozoa of fathers and sons – carriers of reciprocal Chromosome Translocation (RCT)
    BMC Medical Genomics, 2019
    Co-Authors: Marta Olszewska, Alina T. Midro, Ewa Wiland, Nataliya Huleyuk, Monika Fraczek, Danuta Zastavna, Maciej Kurpisz
    Abstract:

    Background Non-random Chromosome positioning has been observed in the nuclei of several different tissue types, including human spermatozoa. The nuclear arrangement of Chromosomes can be altered in men with decreased semen parameters or increased DNA fragmentation and in males with chromosomal numerical or structural aberrations. An aim of this study was to determine whether and how the positioning of nine Chromosome centromeres was (re)arranged in the spermatozoa of fathers and sons – carriers of the same reciprocal Chromosome Translocation (RCT). Methods Fluorescence in situ hybridization (FISH) was applied to analyse the positioning of sperm Chromosomes in a group of 13 carriers of 11 RCTs, including two familial RCT cases: t(4;5) and t(7;10), followed by analysis of eight control individuals. Additionally, sperm chromatin integrity was evaluated using TUNEL and Aniline Blue techniques. Results In the analysed familial RCT cases, repositioning of the Chromosomes occurred in a similar way when compared to the data generated in healthy controls, even if some differences between father and son were further observed. These differences might have arisen from various statuses of sperm chromatin disintegration. Conclusions Nuclear topology appears as another aspect of epigenetic genomic regulation that may influence DNA functioning. We have re-documented that chromosomal positioning is defined in control males and that a particular RCT is reflected in the individual pattern of chromosomal topology. The present study examining the collected RCT group, including two familial cases, additionally showed that chromosomal factors (karyotype and hyperhaploidy) have superior effects, strongly influencing the chromosomal topology, when confronted with sperm chromatin integrity components (DNA fragmentation or chromatin deprotamination).

Justin D. Faris - One of the best experts on this subject based on the ideXlab platform.

  • Molecular and cytogenetic characterization of a durum wheat–Aegilops speltoides Chromosome Translocation conferring resistance to stem rust
    Chromosome Research, 2008
    Co-Authors: Justin D. Faris, Xiwen Cai, Timothy L. Friesen, Yue Jin
    Abstract:

    Stem rust is a serious disease of wheat that has caused historical epidemics, but it has not been a threat in recent decades in North America owing to the eradication of the alternative host and deployment of resistant cultivars. However, the recent emergence of Ug99 (or race TTKS) poses a threat to global wheat production because most currently grown wheat varieties are susceptible. In this study, we evaluated a durum wheat– Aegilops speltoides Chromosome Translocation line (DAS15) for reaction to Ug99 and six other races of stem rust, and used molecular and cytogenetic tools to characterize the Translocation. DAS15 was resistant to all seven races of stem rust. Two durum– Ae. speltoides translocated Chromosomes were detected in DAS15. One Translocation involved the short arm, centromere, and a major portion of the long arm of Ae . speltoides Chromosome 2S and a small terminal segment from durum Chromosome arm 2BL. Thus, this translocated Chromosome is designated T2BL-2SL•2SS. Cytogenetic mapping assigned the resistance gene(s) in DAS15 to the Ae. speltoides segment in T2BL-2SL•2SS. The Ae. speltoides segment in the other translocated Chromosome did not harbour stem rust resistance. A comparison of DAS15 and the wheat stocks carrying the Ae. speltoides -derived resistance genes Sr32 and Sr39 indicated that stem rust resistance gene present in DAS15 is likely novel and will be useful for developing germplasm with resistance to Ug99. Efforts to reduce Ae. speltoides chromatin in T2BL-2SL•2SS are currently in progress.

  • molecular and cytogenetic characterization of a durum wheat aegilops speltoides Chromosome Translocation conferring resistance to stem rust
    Chromosome Research, 2008
    Co-Authors: Justin D. Faris, Xiwen Cai, Timothy L. Friesen, Yue Jin
    Abstract:

    Stem rust is a serious disease of wheat that has caused historical epidemics, but it has not been a threat in recent decades in North America owing to the eradication of the alternative host and deployment of resistant cultivars. However, the recent emergence of Ug99 (or race TTKS) poses a threat to global wheat production because most currently grown wheat varieties are susceptible. In this study, we evaluated a durum wheat–Aegilops speltoides Chromosome Translocation line (DAS15) for reaction to Ug99 and six other races of stem rust, and used molecular and cytogenetic tools to characterize the Translocation. DAS15 was resistant to all seven races of stem rust. Two durum–Ae. speltoides translocated Chromosomes were detected in DAS15. One Translocation involved the short arm, centromere, and a major portion of the long arm of Ae. speltoides Chromosome 2S and a small terminal segment from durum Chromosome arm 2BL. Thus, this translocated Chromosome is designated T2BL-2SL•2SS. Cytogenetic mapping assigned the resistance gene(s) in DAS15 to the Ae. speltoides segment in T2BL-2SL•2SS. The Ae. speltoides segment in the other translocated Chromosome did not harbour stem rust resistance. A comparison of DAS15 and the wheat stocks carrying the Ae. speltoides-derived resistance genes Sr32 and Sr39 indicated that stem rust resistance gene present in DAS15 is likely novel and will be useful for developing germplasm with resistance to Ug99. Efforts to reduce Ae. speltoides chromatin in T2BL-2SL•2SS are currently in progress.

Marta Olszewska - One of the best experts on this subject based on the ideXlab platform.

  • Chromosome re positioning in spermatozoa of fathers and sons carriers of reciprocal Chromosome Translocation rct
    BMC Medical Genomics, 2019
    Co-Authors: Marta Olszewska, Alina T. Midro, Ewa Wiland, Nataliya Huleyuk, Monika Fraczek, Danuta Zastavna, Maciej Kurpisz
    Abstract:

    Non-random Chromosome positioning has been observed in the nuclei of several different tissue types, including human spermatozoa. The nuclear arrangement of Chromosomes can be altered in men with decreased semen parameters or increased DNA fragmentation and in males with chromosomal numerical or structural aberrations. An aim of this study was to determine whether and how the positioning of nine Chromosome centromeres was (re)arranged in the spermatozoa of fathers and sons – carriers of the same reciprocal Chromosome Translocation (RCT). Fluorescence in situ hybridization (FISH) was applied to analyse the positioning of sperm Chromosomes in a group of 13 carriers of 11 RCTs, including two familial RCT cases: t(4;5) and t(7;10), followed by analysis of eight control individuals. Additionally, sperm chromatin integrity was evaluated using TUNEL and Aniline Blue techniques. In the analysed familial RCT cases, repositioning of the Chromosomes occurred in a similar way when compared to the data generated in healthy controls, even if some differences between father and son were further observed. These differences might have arisen from various statuses of sperm chromatin disintegration. Nuclear topology appears as another aspect of epigenetic genomic regulation that may influence DNA functioning. We have re-documented that chromosomal positioning is defined in control males and that a particular RCT is reflected in the individual pattern of chromosomal topology. The present study examining the collected RCT group, including two familial cases, additionally showed that chromosomal factors (karyotype and hyperhaploidy) have superior effects, strongly influencing the chromosomal topology, when confronted with sperm chromatin integrity components (DNA fragmentation or chromatin deprotamination).

  • Chromosome (re)positioning in spermatozoa of fathers and sons – carriers of reciprocal Chromosome Translocation (RCT)
    BMC Medical Genomics, 2019
    Co-Authors: Marta Olszewska, Alina T. Midro, Ewa Wiland, Nataliya Huleyuk, Monika Fraczek, Danuta Zastavna, Maciej Kurpisz
    Abstract:

    Non-random Chromosome positioning has been observed in the nuclei of several different tissue types, including human spermatozoa. The nuclear arrangement of Chromosomes can be altered in men with decreased semen parameters or increased DNA fragmentation and in males with chromosomal numerical or structural aberrations. An aim of this study was to determine whether and how the positioning of nine Chromosome centromeres was (re)arranged in the spermatozoa of fathers and sons – carriers of the same reciprocal Chromosome Translocation (RCT). Fluorescence in situ hybridization (FISH) was applied to analyse the positioning of sperm Chromosomes in a group of 13 carriers of 11 RCTs, including two familial RCT cases: t(4;5) and t(7;10), followed by analysis of eight control individuals. Additionally, sperm chromatin integrity was evaluated using TUNEL and Aniline Blue techniques. In the analysed familial RCT cases, repositioning of the Chromosomes occurred in a similar way when compared to the data generated in healthy controls, even if some differences between father and son were further observed. These differences might have arisen from various statuses of sperm chromatin disintegration. Nuclear topology appears as another aspect of epigenetic genomic regulation that may influence DNA functioning. We have re-documented that chromosomal positioning is defined in control males and that a particular RCT is reflected in the individual pattern of chromosomal topology. The present study examining the collected RCT group, including two familial cases, additionally showed that chromosomal factors (karyotype and hyperhaploidy) have superior effects, strongly influencing the chromosomal topology, when confronted with sperm chromatin integrity components (DNA fragmentation or chromatin deprotamination).

  • Chromosome (re)positioning in spermatozoa of fathers and sons – carriers of reciprocal Chromosome Translocation (RCT)
    BMC Medical Genomics, 2019
    Co-Authors: Marta Olszewska, Alina T. Midro, Ewa Wiland, Nataliya Huleyuk, Monika Fraczek, Danuta Zastavna, Maciej Kurpisz
    Abstract:

    Background Non-random Chromosome positioning has been observed in the nuclei of several different tissue types, including human spermatozoa. The nuclear arrangement of Chromosomes can be altered in men with decreased semen parameters or increased DNA fragmentation and in males with chromosomal numerical or structural aberrations. An aim of this study was to determine whether and how the positioning of nine Chromosome centromeres was (re)arranged in the spermatozoa of fathers and sons – carriers of the same reciprocal Chromosome Translocation (RCT). Methods Fluorescence in situ hybridization (FISH) was applied to analyse the positioning of sperm Chromosomes in a group of 13 carriers of 11 RCTs, including two familial RCT cases: t(4;5) and t(7;10), followed by analysis of eight control individuals. Additionally, sperm chromatin integrity was evaluated using TUNEL and Aniline Blue techniques. Results In the analysed familial RCT cases, repositioning of the Chromosomes occurred in a similar way when compared to the data generated in healthy controls, even if some differences between father and son were further observed. These differences might have arisen from various statuses of sperm chromatin disintegration. Conclusions Nuclear topology appears as another aspect of epigenetic genomic regulation that may influence DNA functioning. We have re-documented that chromosomal positioning is defined in control males and that a particular RCT is reflected in the individual pattern of chromosomal topology. The present study examining the collected RCT group, including two familial cases, additionally showed that chromosomal factors (karyotype and hyperhaploidy) have superior effects, strongly influencing the chromosomal topology, when confronted with sperm chromatin integrity components (DNA fragmentation or chromatin deprotamination).

Danuta Zastavna - One of the best experts on this subject based on the ideXlab platform.

  • Chromosome re positioning in spermatozoa of fathers and sons carriers of reciprocal Chromosome Translocation rct
    BMC Medical Genomics, 2019
    Co-Authors: Marta Olszewska, Alina T. Midro, Ewa Wiland, Nataliya Huleyuk, Monika Fraczek, Danuta Zastavna, Maciej Kurpisz
    Abstract:

    Non-random Chromosome positioning has been observed in the nuclei of several different tissue types, including human spermatozoa. The nuclear arrangement of Chromosomes can be altered in men with decreased semen parameters or increased DNA fragmentation and in males with chromosomal numerical or structural aberrations. An aim of this study was to determine whether and how the positioning of nine Chromosome centromeres was (re)arranged in the spermatozoa of fathers and sons – carriers of the same reciprocal Chromosome Translocation (RCT). Fluorescence in situ hybridization (FISH) was applied to analyse the positioning of sperm Chromosomes in a group of 13 carriers of 11 RCTs, including two familial RCT cases: t(4;5) and t(7;10), followed by analysis of eight control individuals. Additionally, sperm chromatin integrity was evaluated using TUNEL and Aniline Blue techniques. In the analysed familial RCT cases, repositioning of the Chromosomes occurred in a similar way when compared to the data generated in healthy controls, even if some differences between father and son were further observed. These differences might have arisen from various statuses of sperm chromatin disintegration. Nuclear topology appears as another aspect of epigenetic genomic regulation that may influence DNA functioning. We have re-documented that chromosomal positioning is defined in control males and that a particular RCT is reflected in the individual pattern of chromosomal topology. The present study examining the collected RCT group, including two familial cases, additionally showed that chromosomal factors (karyotype and hyperhaploidy) have superior effects, strongly influencing the chromosomal topology, when confronted with sperm chromatin integrity components (DNA fragmentation or chromatin deprotamination).

  • Chromosome (re)positioning in spermatozoa of fathers and sons – carriers of reciprocal Chromosome Translocation (RCT)
    BMC Medical Genomics, 2019
    Co-Authors: Marta Olszewska, Alina T. Midro, Ewa Wiland, Nataliya Huleyuk, Monika Fraczek, Danuta Zastavna, Maciej Kurpisz
    Abstract:

    Non-random Chromosome positioning has been observed in the nuclei of several different tissue types, including human spermatozoa. The nuclear arrangement of Chromosomes can be altered in men with decreased semen parameters or increased DNA fragmentation and in males with chromosomal numerical or structural aberrations. An aim of this study was to determine whether and how the positioning of nine Chromosome centromeres was (re)arranged in the spermatozoa of fathers and sons – carriers of the same reciprocal Chromosome Translocation (RCT). Fluorescence in situ hybridization (FISH) was applied to analyse the positioning of sperm Chromosomes in a group of 13 carriers of 11 RCTs, including two familial RCT cases: t(4;5) and t(7;10), followed by analysis of eight control individuals. Additionally, sperm chromatin integrity was evaluated using TUNEL and Aniline Blue techniques. In the analysed familial RCT cases, repositioning of the Chromosomes occurred in a similar way when compared to the data generated in healthy controls, even if some differences between father and son were further observed. These differences might have arisen from various statuses of sperm chromatin disintegration. Nuclear topology appears as another aspect of epigenetic genomic regulation that may influence DNA functioning. We have re-documented that chromosomal positioning is defined in control males and that a particular RCT is reflected in the individual pattern of chromosomal topology. The present study examining the collected RCT group, including two familial cases, additionally showed that chromosomal factors (karyotype and hyperhaploidy) have superior effects, strongly influencing the chromosomal topology, when confronted with sperm chromatin integrity components (DNA fragmentation or chromatin deprotamination).

  • Chromosome (re)positioning in spermatozoa of fathers and sons – carriers of reciprocal Chromosome Translocation (RCT)
    BMC Medical Genomics, 2019
    Co-Authors: Marta Olszewska, Alina T. Midro, Ewa Wiland, Nataliya Huleyuk, Monika Fraczek, Danuta Zastavna, Maciej Kurpisz
    Abstract:

    Background Non-random Chromosome positioning has been observed in the nuclei of several different tissue types, including human spermatozoa. The nuclear arrangement of Chromosomes can be altered in men with decreased semen parameters or increased DNA fragmentation and in males with chromosomal numerical or structural aberrations. An aim of this study was to determine whether and how the positioning of nine Chromosome centromeres was (re)arranged in the spermatozoa of fathers and sons – carriers of the same reciprocal Chromosome Translocation (RCT). Methods Fluorescence in situ hybridization (FISH) was applied to analyse the positioning of sperm Chromosomes in a group of 13 carriers of 11 RCTs, including two familial RCT cases: t(4;5) and t(7;10), followed by analysis of eight control individuals. Additionally, sperm chromatin integrity was evaluated using TUNEL and Aniline Blue techniques. Results In the analysed familial RCT cases, repositioning of the Chromosomes occurred in a similar way when compared to the data generated in healthy controls, even if some differences between father and son were further observed. These differences might have arisen from various statuses of sperm chromatin disintegration. Conclusions Nuclear topology appears as another aspect of epigenetic genomic regulation that may influence DNA functioning. We have re-documented that chromosomal positioning is defined in control males and that a particular RCT is reflected in the individual pattern of chromosomal topology. The present study examining the collected RCT group, including two familial cases, additionally showed that chromosomal factors (karyotype and hyperhaploidy) have superior effects, strongly influencing the chromosomal topology, when confronted with sperm chromatin integrity components (DNA fragmentation or chromatin deprotamination).