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Lawan Chanhome - One of the best experts on this subject based on the ideXlab platform.
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Chromosome map of the Siamese cobra: did partial synteny of sex Chromosomes in the amniote represent “a hypothetical ancestral super-sex Chromosome” or random distribution?
BMC Genomics, 2018Co-Authors: Worapong Singchat, Siwapech Sillapaprayoon, Narongrit Muangmai, Sudarath Baicharoen, Surin Peyachoknagul, Rebecca E. O’connor, Panupong Tawichasri, Aorarat Suntronpong, Sunutcha Suntrarachun, Lawan ChanhomeAbstract:Background Unlike the Chromosome constitution of most snakes (2n=36), the cobra karyotype shows a diploid Chromosome number of 38 with a highly heterochromatic W Chromosome and a large morphologically different Chromosome 2. To investigate the process of sex Chromosome differentiation and evolution between cobras, most snakes, and other amniotes, we constructed a Chromosome map of the Siamese cobra ( Naja kaouthia ) with 43 bacterial artificial Chromosomes (BACs) derived from the chicken and zebra finch libraries using the fluorescence in situ hybridization (FISH) technique, and compared it with those of the chicken, the zebra finch, and other amniotes. Results We produced a detailed Chromosome map of the Siamese cobra genome, focusing on Chromosome 2 and sex Chromosomes. Synteny of the Siamese cobra Chromosome 2 (NKA2) and NKAZ were highly conserved among snakes and other squamate reptiles, except for intrachromosomal rearrangements occurring in NKA2. Interestingly, twelve BACs that had partial homology with sex Chromosomes of several amniotes were mapped on the heterochromatic NKAW as hybridization signals such as repeat sequences. Sequence analysis showed that most of these BACs contained high proportions of transposable elements. In addition, hybridization signals of telomeric repeat (TTAGGG)_n and six microsatellite repeat motifs ((AAGG)_8, (AGAT)_8, (AAAC)_8, (ACAG)_8, (AATC)_8, and (AAAAT)_6) were observed on NKAW, and most of these were also found on other amniote sex Chromosomes. Conclusions The frequent amplification of repeats might involve heterochromatinization and promote sex Chromosome differentiation in the Siamese cobra W sex Chromosome. Repeat sequences are also shared among amniote sex Chromosomes, which supports the hypothesis of an ancestral super-sex Chromosome with overlaps of partial syntenies. Alternatively, amplification of microsatellite repeat motifs could have occurred independently in each lineage, representing convergent sex chromosomal differentiation among amniote sex Chromosomes.
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Chromosome map of the siamese cobra did partial synteny of sex Chromosomes in the amniote represent a hypothetical ancestral super sex Chromosome or random distribution
BMC Genomics, 2018Co-Authors: Worapong Singchat, Siwapech Sillapaprayoon, Narongrit Muangmai, Sudarath Baicharoen, Surin Peyachoknagul, Rebecca E Oconnor, Panupong Tawichasri, Aorarat Suntronpong, Sunutcha Suntrarachun, Lawan ChanhomeAbstract:Unlike the Chromosome constitution of most snakes (2n=36), the cobra karyotype shows a diploid Chromosome number of 38 with a highly heterochromatic W Chromosome and a large morphologically different Chromosome 2. To investigate the process of sex Chromosome differentiation and evolution between cobras, most snakes, and other amniotes, we constructed a Chromosome map of the Siamese cobra (Naja kaouthia) with 43 bacterial artificial Chromosomes (BACs) derived from the chicken and zebra finch libraries using the fluorescence in situ hybridization (FISH) technique, and compared it with those of the chicken, the zebra finch, and other amniotes. We produced a detailed Chromosome map of the Siamese cobra genome, focusing on Chromosome 2 and sex Chromosomes. Synteny of the Siamese cobra Chromosome 2 (NKA2) and NKAZ were highly conserved among snakes and other squamate reptiles, except for intrachromosomal rearrangements occurring in NKA2. Interestingly, twelve BACs that had partial homology with sex Chromosomes of several amniotes were mapped on the heterochromatic NKAW as hybridization signals such as repeat sequences. Sequence analysis showed that most of these BACs contained high proportions of transposable elements. In addition, hybridization signals of telomeric repeat (TTAGGG)n and six microsatellite repeat motifs ((AAGG)8, (AGAT)8, (AAAC)8, (ACAG)8, (AATC)8, and (AAAAT)6) were observed on NKAW, and most of these were also found on other amniote sex Chromosomes. The frequent amplification of repeats might involve heterochromatinization and promote sex Chromosome differentiation in the Siamese cobra W sex Chromosome. Repeat sequences are also shared among amniote sex Chromosomes, which supports the hypothesis of an ancestral super-sex Chromosome with overlaps of partial syntenies. Alternatively, amplification of microsatellite repeat motifs could have occurred independently in each lineage, representing convergent sex chromosomal differentiation among amniote sex Chromosomes.
M Ponsa - One of the best experts on this subject based on the ideXlab platform.
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characterization of constitutive heterochromatin in cebus apella cebidae primates and pan troglodytes hominidae primates comparison to human Chromosomes
American Journal of Primatology, 1999Co-Authors: Francisca Maria Ferrando Garcia, Carme Nogues, M Garcia, J Egozcue, M PonsaAbstract:Using G bands, some homologies between the Chromosomes of Cebus apella (CAP) and human Chromosomes are difficult to establish. To solve this problem, we analyzed these homologies by fluorescence in situ hybridization using human whole Chromosome probes (ZOO-FISH). The results indicated that 1) the human probe for Chromosome 2 partially hybridizes with CAP Chromosomes 13 and 5, 2) the human probe for Chromosome 3 partially hybridizes with CAP Chromosomes 18 and 20, 3) the human probe for Chromosome 9 partially hibridizes with CAP Chromosome 19, and 4) the human probe for Chromosome 14 hybridizes with the p-terminal and q-terminal regions of CAP Chromosome 6. However, none of the human probes employed hybridized with the heterochromatic regions of CAP Chromosomes. For this reason, we characterized the heterochromatic regions of CAP Chromosomes and of the Chromosomes of Pan troglodytes (PTR), to allow comparison between CAP, PTR, and human Chromosomes using in situ digestion of fixed Chromosomes with the restriction enzymes AluI, HaeIII, and RsaI and by fluorescent staining with DA/DAPI. The results show that 1) centromeric heterochromatin is heterogeneous in the three species studied and 2) noncentromeric heterochromatin is homogeneous within each of the three species, but is different for each species. Thus, centromeric heterochromatin undergoes a higher degree of variability than noncentromeric heterochromatin. Am. J. Primatol. 49:205–221, 1999. © 1999 Wiley-Liss, Inc.
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characterization of constitutive heterochromatin in cebus apella cebidae primates and pan troglodytes hominidae primates comparison to human Chromosomes
American Journal of Primatology, 1999Co-Authors: Francisca Garcia, Carme Nogues, M Garcia, J Egozcue, M PonsaAbstract:Using G bands, some homologies between the Chromosomes of Cebus apella (CAP) and human Chromosomes are difficult to establish. To solve this problem, we analyzed these homologies by fluorescence in situ hybridization using human whole Chromosome probes (ZOO-FISH). The results indicated that 1) the human probe for Chromosome 2 partially hybridizes with CAP Chromosomes 13 and 5, 2) the human probe for Chromosome 3 partially hybridizes with CAP Chromosomes 18 and 20, 3) the human probe for Chromosome 9 partially hybridizes with CAP Chromosome 19, and 4) the human probe for Chromosome 14 hybridizes with the p-terminal and q-terminal regions of CAP Chromosome 6. However, none of the human probes employed hybridized with the heterochromatic regions of CAP Chromosomes. For this reason, we characterized the heterochromatic regions of CAP Chromosomes and of the Chromosomes of Pan troglodytes (PTR), to allow comparison between CAP, PTR, and human Chromosomes using in situ digestion of fixed Chromosomes with the restriction enzymes AluI, HaeIII, and RsaI and by fluorescent staining with DA/DAPI. The results show that 1) centromeric heterochromatin is heterogeneous in the three species studied and 2) noncentromeric heterochromatin is homogeneous within each of the three species, but is different for each species. Thus, centromeric heterochromatin undergoes a higher degree of variability than noncentromeric heterochromatin.
Worapong Singchat - One of the best experts on this subject based on the ideXlab platform.
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Chromosome map of the Siamese cobra: did partial synteny of sex Chromosomes in the amniote represent “a hypothetical ancestral super-sex Chromosome” or random distribution?
BMC Genomics, 2018Co-Authors: Worapong Singchat, Siwapech Sillapaprayoon, Narongrit Muangmai, Sudarath Baicharoen, Surin Peyachoknagul, Rebecca E. O’connor, Panupong Tawichasri, Aorarat Suntronpong, Sunutcha Suntrarachun, Lawan ChanhomeAbstract:Background Unlike the Chromosome constitution of most snakes (2n=36), the cobra karyotype shows a diploid Chromosome number of 38 with a highly heterochromatic W Chromosome and a large morphologically different Chromosome 2. To investigate the process of sex Chromosome differentiation and evolution between cobras, most snakes, and other amniotes, we constructed a Chromosome map of the Siamese cobra ( Naja kaouthia ) with 43 bacterial artificial Chromosomes (BACs) derived from the chicken and zebra finch libraries using the fluorescence in situ hybridization (FISH) technique, and compared it with those of the chicken, the zebra finch, and other amniotes. Results We produced a detailed Chromosome map of the Siamese cobra genome, focusing on Chromosome 2 and sex Chromosomes. Synteny of the Siamese cobra Chromosome 2 (NKA2) and NKAZ were highly conserved among snakes and other squamate reptiles, except for intrachromosomal rearrangements occurring in NKA2. Interestingly, twelve BACs that had partial homology with sex Chromosomes of several amniotes were mapped on the heterochromatic NKAW as hybridization signals such as repeat sequences. Sequence analysis showed that most of these BACs contained high proportions of transposable elements. In addition, hybridization signals of telomeric repeat (TTAGGG)_n and six microsatellite repeat motifs ((AAGG)_8, (AGAT)_8, (AAAC)_8, (ACAG)_8, (AATC)_8, and (AAAAT)_6) were observed on NKAW, and most of these were also found on other amniote sex Chromosomes. Conclusions The frequent amplification of repeats might involve heterochromatinization and promote sex Chromosome differentiation in the Siamese cobra W sex Chromosome. Repeat sequences are also shared among amniote sex Chromosomes, which supports the hypothesis of an ancestral super-sex Chromosome with overlaps of partial syntenies. Alternatively, amplification of microsatellite repeat motifs could have occurred independently in each lineage, representing convergent sex chromosomal differentiation among amniote sex Chromosomes.
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Chromosome map of the siamese cobra did partial synteny of sex Chromosomes in the amniote represent a hypothetical ancestral super sex Chromosome or random distribution
BMC Genomics, 2018Co-Authors: Worapong Singchat, Siwapech Sillapaprayoon, Narongrit Muangmai, Sudarath Baicharoen, Surin Peyachoknagul, Rebecca E Oconnor, Panupong Tawichasri, Aorarat Suntronpong, Sunutcha Suntrarachun, Lawan ChanhomeAbstract:Unlike the Chromosome constitution of most snakes (2n=36), the cobra karyotype shows a diploid Chromosome number of 38 with a highly heterochromatic W Chromosome and a large morphologically different Chromosome 2. To investigate the process of sex Chromosome differentiation and evolution between cobras, most snakes, and other amniotes, we constructed a Chromosome map of the Siamese cobra (Naja kaouthia) with 43 bacterial artificial Chromosomes (BACs) derived from the chicken and zebra finch libraries using the fluorescence in situ hybridization (FISH) technique, and compared it with those of the chicken, the zebra finch, and other amniotes. We produced a detailed Chromosome map of the Siamese cobra genome, focusing on Chromosome 2 and sex Chromosomes. Synteny of the Siamese cobra Chromosome 2 (NKA2) and NKAZ were highly conserved among snakes and other squamate reptiles, except for intrachromosomal rearrangements occurring in NKA2. Interestingly, twelve BACs that had partial homology with sex Chromosomes of several amniotes were mapped on the heterochromatic NKAW as hybridization signals such as repeat sequences. Sequence analysis showed that most of these BACs contained high proportions of transposable elements. In addition, hybridization signals of telomeric repeat (TTAGGG)n and six microsatellite repeat motifs ((AAGG)8, (AGAT)8, (AAAC)8, (ACAG)8, (AATC)8, and (AAAAT)6) were observed on NKAW, and most of these were also found on other amniote sex Chromosomes. The frequent amplification of repeats might involve heterochromatinization and promote sex Chromosome differentiation in the Siamese cobra W sex Chromosome. Repeat sequences are also shared among amniote sex Chromosomes, which supports the hypothesis of an ancestral super-sex Chromosome with overlaps of partial syntenies. Alternatively, amplification of microsatellite repeat motifs could have occurred independently in each lineage, representing convergent sex chromosomal differentiation among amniote sex Chromosomes.
Narongrit Muangmai - One of the best experts on this subject based on the ideXlab platform.
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Chromosome map of the Siamese cobra: did partial synteny of sex Chromosomes in the amniote represent “a hypothetical ancestral super-sex Chromosome” or random distribution?
BMC Genomics, 2018Co-Authors: Worapong Singchat, Siwapech Sillapaprayoon, Narongrit Muangmai, Sudarath Baicharoen, Surin Peyachoknagul, Rebecca E. O’connor, Panupong Tawichasri, Aorarat Suntronpong, Sunutcha Suntrarachun, Lawan ChanhomeAbstract:Background Unlike the Chromosome constitution of most snakes (2n=36), the cobra karyotype shows a diploid Chromosome number of 38 with a highly heterochromatic W Chromosome and a large morphologically different Chromosome 2. To investigate the process of sex Chromosome differentiation and evolution between cobras, most snakes, and other amniotes, we constructed a Chromosome map of the Siamese cobra ( Naja kaouthia ) with 43 bacterial artificial Chromosomes (BACs) derived from the chicken and zebra finch libraries using the fluorescence in situ hybridization (FISH) technique, and compared it with those of the chicken, the zebra finch, and other amniotes. Results We produced a detailed Chromosome map of the Siamese cobra genome, focusing on Chromosome 2 and sex Chromosomes. Synteny of the Siamese cobra Chromosome 2 (NKA2) and NKAZ were highly conserved among snakes and other squamate reptiles, except for intrachromosomal rearrangements occurring in NKA2. Interestingly, twelve BACs that had partial homology with sex Chromosomes of several amniotes were mapped on the heterochromatic NKAW as hybridization signals such as repeat sequences. Sequence analysis showed that most of these BACs contained high proportions of transposable elements. In addition, hybridization signals of telomeric repeat (TTAGGG)_n and six microsatellite repeat motifs ((AAGG)_8, (AGAT)_8, (AAAC)_8, (ACAG)_8, (AATC)_8, and (AAAAT)_6) were observed on NKAW, and most of these were also found on other amniote sex Chromosomes. Conclusions The frequent amplification of repeats might involve heterochromatinization and promote sex Chromosome differentiation in the Siamese cobra W sex Chromosome. Repeat sequences are also shared among amniote sex Chromosomes, which supports the hypothesis of an ancestral super-sex Chromosome with overlaps of partial syntenies. Alternatively, amplification of microsatellite repeat motifs could have occurred independently in each lineage, representing convergent sex chromosomal differentiation among amniote sex Chromosomes.
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Chromosome map of the siamese cobra did partial synteny of sex Chromosomes in the amniote represent a hypothetical ancestral super sex Chromosome or random distribution
BMC Genomics, 2018Co-Authors: Worapong Singchat, Siwapech Sillapaprayoon, Narongrit Muangmai, Sudarath Baicharoen, Surin Peyachoknagul, Rebecca E Oconnor, Panupong Tawichasri, Aorarat Suntronpong, Sunutcha Suntrarachun, Lawan ChanhomeAbstract:Unlike the Chromosome constitution of most snakes (2n=36), the cobra karyotype shows a diploid Chromosome number of 38 with a highly heterochromatic W Chromosome and a large morphologically different Chromosome 2. To investigate the process of sex Chromosome differentiation and evolution between cobras, most snakes, and other amniotes, we constructed a Chromosome map of the Siamese cobra (Naja kaouthia) with 43 bacterial artificial Chromosomes (BACs) derived from the chicken and zebra finch libraries using the fluorescence in situ hybridization (FISH) technique, and compared it with those of the chicken, the zebra finch, and other amniotes. We produced a detailed Chromosome map of the Siamese cobra genome, focusing on Chromosome 2 and sex Chromosomes. Synteny of the Siamese cobra Chromosome 2 (NKA2) and NKAZ were highly conserved among snakes and other squamate reptiles, except for intrachromosomal rearrangements occurring in NKA2. Interestingly, twelve BACs that had partial homology with sex Chromosomes of several amniotes were mapped on the heterochromatic NKAW as hybridization signals such as repeat sequences. Sequence analysis showed that most of these BACs contained high proportions of transposable elements. In addition, hybridization signals of telomeric repeat (TTAGGG)n and six microsatellite repeat motifs ((AAGG)8, (AGAT)8, (AAAC)8, (ACAG)8, (AATC)8, and (AAAAT)6) were observed on NKAW, and most of these were also found on other amniote sex Chromosomes. The frequent amplification of repeats might involve heterochromatinization and promote sex Chromosome differentiation in the Siamese cobra W sex Chromosome. Repeat sequences are also shared among amniote sex Chromosomes, which supports the hypothesis of an ancestral super-sex Chromosome with overlaps of partial syntenies. Alternatively, amplification of microsatellite repeat motifs could have occurred independently in each lineage, representing convergent sex chromosomal differentiation among amniote sex Chromosomes.
Panupong Tawichasri - One of the best experts on this subject based on the ideXlab platform.
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Chromosome map of the Siamese cobra: did partial synteny of sex Chromosomes in the amniote represent “a hypothetical ancestral super-sex Chromosome” or random distribution?
BMC Genomics, 2018Co-Authors: Worapong Singchat, Siwapech Sillapaprayoon, Narongrit Muangmai, Sudarath Baicharoen, Surin Peyachoknagul, Rebecca E. O’connor, Panupong Tawichasri, Aorarat Suntronpong, Sunutcha Suntrarachun, Lawan ChanhomeAbstract:Background Unlike the Chromosome constitution of most snakes (2n=36), the cobra karyotype shows a diploid Chromosome number of 38 with a highly heterochromatic W Chromosome and a large morphologically different Chromosome 2. To investigate the process of sex Chromosome differentiation and evolution between cobras, most snakes, and other amniotes, we constructed a Chromosome map of the Siamese cobra ( Naja kaouthia ) with 43 bacterial artificial Chromosomes (BACs) derived from the chicken and zebra finch libraries using the fluorescence in situ hybridization (FISH) technique, and compared it with those of the chicken, the zebra finch, and other amniotes. Results We produced a detailed Chromosome map of the Siamese cobra genome, focusing on Chromosome 2 and sex Chromosomes. Synteny of the Siamese cobra Chromosome 2 (NKA2) and NKAZ were highly conserved among snakes and other squamate reptiles, except for intrachromosomal rearrangements occurring in NKA2. Interestingly, twelve BACs that had partial homology with sex Chromosomes of several amniotes were mapped on the heterochromatic NKAW as hybridization signals such as repeat sequences. Sequence analysis showed that most of these BACs contained high proportions of transposable elements. In addition, hybridization signals of telomeric repeat (TTAGGG)_n and six microsatellite repeat motifs ((AAGG)_8, (AGAT)_8, (AAAC)_8, (ACAG)_8, (AATC)_8, and (AAAAT)_6) were observed on NKAW, and most of these were also found on other amniote sex Chromosomes. Conclusions The frequent amplification of repeats might involve heterochromatinization and promote sex Chromosome differentiation in the Siamese cobra W sex Chromosome. Repeat sequences are also shared among amniote sex Chromosomes, which supports the hypothesis of an ancestral super-sex Chromosome with overlaps of partial syntenies. Alternatively, amplification of microsatellite repeat motifs could have occurred independently in each lineage, representing convergent sex chromosomal differentiation among amniote sex Chromosomes.
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Chromosome map of the siamese cobra did partial synteny of sex Chromosomes in the amniote represent a hypothetical ancestral super sex Chromosome or random distribution
BMC Genomics, 2018Co-Authors: Worapong Singchat, Siwapech Sillapaprayoon, Narongrit Muangmai, Sudarath Baicharoen, Surin Peyachoknagul, Rebecca E Oconnor, Panupong Tawichasri, Aorarat Suntronpong, Sunutcha Suntrarachun, Lawan ChanhomeAbstract:Unlike the Chromosome constitution of most snakes (2n=36), the cobra karyotype shows a diploid Chromosome number of 38 with a highly heterochromatic W Chromosome and a large morphologically different Chromosome 2. To investigate the process of sex Chromosome differentiation and evolution between cobras, most snakes, and other amniotes, we constructed a Chromosome map of the Siamese cobra (Naja kaouthia) with 43 bacterial artificial Chromosomes (BACs) derived from the chicken and zebra finch libraries using the fluorescence in situ hybridization (FISH) technique, and compared it with those of the chicken, the zebra finch, and other amniotes. We produced a detailed Chromosome map of the Siamese cobra genome, focusing on Chromosome 2 and sex Chromosomes. Synteny of the Siamese cobra Chromosome 2 (NKA2) and NKAZ were highly conserved among snakes and other squamate reptiles, except for intrachromosomal rearrangements occurring in NKA2. Interestingly, twelve BACs that had partial homology with sex Chromosomes of several amniotes were mapped on the heterochromatic NKAW as hybridization signals such as repeat sequences. Sequence analysis showed that most of these BACs contained high proportions of transposable elements. In addition, hybridization signals of telomeric repeat (TTAGGG)n and six microsatellite repeat motifs ((AAGG)8, (AGAT)8, (AAAC)8, (ACAG)8, (AATC)8, and (AAAAT)6) were observed on NKAW, and most of these were also found on other amniote sex Chromosomes. The frequent amplification of repeats might involve heterochromatinization and promote sex Chromosome differentiation in the Siamese cobra W sex Chromosome. Repeat sequences are also shared among amniote sex Chromosomes, which supports the hypothesis of an ancestral super-sex Chromosome with overlaps of partial syntenies. Alternatively, amplification of microsatellite repeat motifs could have occurred independently in each lineage, representing convergent sex chromosomal differentiation among amniote sex Chromosomes.
