The Experts below are selected from a list of 3123 Experts worldwide ranked by ideXlab platform

Takashi Fujikado - One of the best experts on this subject based on the ideXlab platform.

  • Asymmetric Wavefront Aberrations and Pupillary Shapes Induced by Electrical Stimulation of Ciliary Nerve in Cats Measured with Compact Wavefront Aberrometer
    2016
    Co-Authors: Suguru Miyagawa, Tomomitsu Miyoshi, Takeshi Morimoto, Yoko Hirohara, Toshifumi Mihashi, Takao Endo, Hiroyuki K, Takashi Fujikado
    Abstract:

    To investigate the changes in the wavefront aberrations and pupillary shape in response to electrical stimulation of the branches of the Ciliary Nerves in cats. Seven eyes of seven cats were studied under general anesthesia. Trains of monophasic pulses (current, 0.1 to 1.0 mA; duration, 0.5 ms/phase; frequency, 5 to 40 Hz) were applied to the lateral or medial branch of the short Ciliary Nerve near the posterior pole of the eye. A pair of electrodes was hooked onto one or both branch of the short Ciliary Nerve. The electrodes were placed about 5 mm from the scleral surface. The wavefront aberrations were recorded continuously for 2 seconds before, 8 seconds during, and for 20 seconds after the electrical stimulation. The pupillary images were simultaneously recorded during the stimulation period. Both the wavefront aberrations and the pupillary images were obtained 10 times/sec with a custom-built wavefront aberrometer. The maximum accommodative amplitude was 1.19 diopters (D) produced by electrical stimulation of the short Ciliary Nerves. The latency of the accommodative changes was very short, and the accommodative level gradually increased up to 4 seconds and reached a plateau. When only one branch of the Ciliary Nerve was stimulated, the pupil dilated asymmetrically, and the oblique astigmatism and one of the asymmetrical wavefront terms was also altered. Our results showed that the wavefront aberrations and pupillary dilations can be measured simultaneously and serially with a compact wavefront aberrometer. The asymmetric pupil dilation and asymmetric changes of the wavefront aberrations suggest that each branch of the ciliar

  • Asymmetric wavefront aberrations and pupillary shapes induced by electrical stimulation of Ciliary Nerve in cats measured with compact wavefront aberrometer.
    PLOS ONE, 2014
    Co-Authors: Suguru Miyagawa, Hiroyuki Kanda, Tomomitsu Miyoshi, Takeshi Morimoto, Yoko Hirohara, Toshifumi Mihashi, Takao Endo, Takashi Fujikado
    Abstract:

    : To investigate the changes in the wavefront aberrations and pupillary shape in response to electrical stimulation of the branches of the Ciliary Nerves in cats. Seven eyes of seven cats were studied under general anesthesia. Trains of monophasic pulses (current, 0.1 to 1.0 mA; duration, 0.5 ms/phase; frequency, 5 to 40 Hz) were applied to the lateral or medial branch of the short Ciliary Nerve near the posterior pole of the eye. A pair of electrodes was hooked onto one or both branch of the short Ciliary Nerve. The electrodes were placed about 5 mm from the scleral surface. The wavefront aberrations were recorded continuously for 2 seconds before, 8 seconds during, and for 20 seconds after the electrical stimulation. The pupillary images were simultaneously recorded during the stimulation period. Both the wavefront aberrations and the pupillary images were obtained 10 times/sec with a custom-built wavefront aberrometer. The maximum accommodative amplitude was 1.19 diopters (D) produced by electrical stimulation of the short Ciliary Nerves. The latency of the accommodative changes was very short, and the accommodative level gradually increased up to 4 seconds and reached a plateau. When only one branch of the Ciliary Nerve was stimulated, the pupil dilated asymmetrically, and the oblique astigmatism and one of the asymmetrical wavefront terms was also altered. Our results showed that the wavefront aberrations and pupillary dilations can be measured simultaneously and serially with a compact wavefront aberrometer. The asymmetric pupil dilation and asymmetric changes of the wavefront aberrations suggest that each branch of the Ciliary Nerve innervates specific segments of the Ciliary muscle and dilator muscle of the pupil.

Takefumi Yamaguchi - One of the best experts on this subject based on the ideXlab platform.

  • Bilateral Nerve Alterations in a Unilateral Experimental Neurotrophic Keratopathy Model: A Lateral Conjunctival Approach for Trigeminal Axotomy
    2016
    Co-Authors: Takefumi Yamaguchi, Aslihan Turhan, Deshea L Harris, Ulrich Von, Andrian Pedram Hamrah
    Abstract:

    To study bilateral Nerve changes in a newly developed novel mouse model for neurotrophic keratopathy by approaching the trigeminal Nerve from the lateral fornix. Surgical axotomy of the Ciliary Nerve of the trigeminal Nerve was performed in adult BALB/c mice at the posterior sclera. Axotomized, contralateral, and sham-treated corneas were excised on post-operative days 1, 3, 5, 7 and 14 and immunofluorescence histochemistry was performed with anti-b-tubulin antibody to evaluate corneal Nerve density. Blink reflex was evaluated using a nylon thread. The survival rate was 100 % with minimal bleeding during axotomy and a surgical time of 860.5 minutes. The blink reflex was diminished at day 1 after axotomy, but remained intact in the contralateral eyes in all mice. The central and peripheral subbasal Nerves were not detectable in the axotomized cornea at day 1 (p,0.001), compared to normal eyes (101.3614.8 and 69.7612.0 mm/mm2 centrally and peripherally). Interestingly, the subbasal Nerve density in the contralateral non-surgical eyes also decreased significantly to 62.462.8 mm/mm2 in the center from day 1 (p,0.001), but did not change in the periphery (77.3611.7 mm/mm2, P = 0.819). Our novel trigeminal axotomy mouse model is highly effective, less invasive, rapid, and has a high survival rate, demonstrating immediate loss of subbasal Nerves in axotomized eyes and decreased subbasal Nerves in contralateral eyes after unilateral axotomy. This model will allow investigating the effects of corneal Nerve damage and serves as a new mode

  • bilateral Nerve alterations in a unilateral experimental neurotrophic keratopathy model a lateral conjunctival approach for trigeminal axotomy
    PLOS ONE, 2013
    Co-Authors: Takefumi Yamaguchi, Aslihan Turhan, Deshea L Harris, Harald Pruss, Kai Hu, Ulrich H Von Andrian, Pedram Hamrah
    Abstract:

    To study bilateral Nerve changes in a newly developed novel mouse model for neurotrophic keratopathy by approaching the trigeminal Nerve from the lateral fornix. Surgical axotomy of the Ciliary Nerve of the trigeminal Nerve was performed in adult BALB/c mice at the posterior sclera. Axotomized, contralateral, and sham-treated corneas were excised on post-operative days 1, 3, 5, 7 and 14 and immunofluorescence histochemistry was performed with anti-β-tubulin antibody to evaluate corneal Nerve density. Blink reflex was evaluated using a nylon thread. The survival rate was 100% with minimal bleeding during axotomy and a surgical time of 8±0.5 minutes. The blink reflex was diminished at day 1 after axotomy, but remained intact in the contralateral eyes in all mice. The central and peripheral subbasal Nerves were not detectable in the axotomized cornea at day 1 (p<0.001), compared to normal eyes (101.3±14.8 and 69.7±12.0 mm/mm2 centrally and peripherally). Interestingly, the subbasal Nerve density in the contralateral non-surgical eyes also decreased significantly to 62.4±2.8 mm/mm2 in the center from day 1 (p<0.001), but did not change in the periphery (77.3±11.7 mm/mm2, P = 0.819). Our novel trigeminal axotomy mouse model is highly effective, less invasive, rapid, and has a high survival rate, demonstrating immediate loss of subbasal Nerves in axotomized eyes and decreased subbasal Nerves in contralateral eyes after unilateral axotomy. This model will allow investigating the effects of corneal Nerve damage and serves as a new model for neurotrophic keratopathy.

Baudouin Christophe - One of the best experts on this subject based on the ideXlab platform.

  • Capsazepine decreases corneal pain syndrome in severe dry eye disease
    'Springer Science and Business Media LLC', 2021
    Co-Authors: Fakih Darine, Guerrero-moreno Adrian, Baudouin Christophe, Goazigo, Annabelle Réaux-le, Parsadaniantz, Stéphane Mélik
    Abstract:

    International audienceBackground: Dry eye disease (DED) is a multifactorial disease of the ocular surface accompanied by neurosensory abnormalities. Here, we evaluated the effectiveness of transient receptor potential vanilloid-1 (TRPV1) blockade to alleviate ocular pain, neuroinflammation, and anxiety-like behavior associated with severe DED.Methods: Chronic DED was induced by unilateral excision of the Harderian and extraorbital lacrimal glands of adult male mice. Investigations were conducted at 21 days after surgery. The mRNA levels of TRPV1, transient receptor potential ankyrin-1 (TRPA1), and acid-sensing ion channels 1 and 3 (ASIC1 and ASIC3) in the trigeminal ganglion (TG) were evaluated by RNAscope in situ hybridization. Multi-unit extracellular recording of Ciliary Nerve fiber activity was used to monitor spontaneous and stimulated (cold, heat, and acid) corneal Nerve responsiveness in ex vivo eye preparations. DED mice received topical instillations of the TRPV1 antagonist (capsazepine) twice a day for 2 weeks from d7 to d21 after surgery. The expression of genes involved in neuropathic and inflammatory pain was evaluated in the TG using a global genomic approach. Chemical and mechanical corneal nociception and spontaneous ocular pain were monitored. Finally, anxiety-like behaviors were assessed by elevated plus maze and black and white box tests.Results: First, in situ hybridization showed DED to trigger upregulation of TRPV1, TRPA1, ASIC1, and ASIC3 mRNA in the ophthalmic branch of the TG. DED also induced overexpression of genes involved in neuropathic and inflammatory pain in the TG. Repeated instillations of capsazepine reduced corneal polymodal responsiveness to heat, cold, and acidic stimulation in ex vivo eye preparations. Consistent with these findings, chronic capsazepine instillation inhibited the upregulation of genes involved in neuropathic and inflammatory pain in the TG of DED animals and reduced the sensation of ocular pain, as well as anxiety-like behaviors associated with severe DED.Conclusion: These data provide novel insights on the effectiveness of TRPV1 antagonist instillation in alleviating abnormal corneal neurosensory symptoms induced by severe DED, opening an avenue for the repositioning of this molecule as a potential analgesic treatment for patients suffering from chronic DED

  • Topical treatment with a mu opioid receptor agonist alleviates corneal allodynia and corneal Nerve sensitization in mice
    'Elsevier BV', 2020
    Co-Authors: Joubert Fanny, Guerrero-moreno Adrian, Fakih Darine, Reboussin Elodie, Gaveriaux-ruff Claire, Acosta Maria, Gallar Juana, Sahel José, Bodineau Laurence, Baudouin Christophe
    Abstract:

    International audienceCorneal pain is considered to be a core symptom of ocular surface disruption and inflammation. The management of this debilitating condition is still a therapeutic challenge. Recent evidence supports a role of the opioid system in the management of corneal nociception. However, the functional involvement of the mu opioid receptor (MOR) underlying this analgesic effect is not known. We first investigated the expression of the MOR in corneal Nerve fibers and trigeminal ganglion (TG) neurons in control mice and a mouse model of corneal inflammatory pain. We then evaluated the anti-nociceptive and electrophysiological effects of DAMGO ([D-Ala2,N-Me-Phe4,Gly5-ol] enkephalin), a MOR-selective ligand. MOR immunoreactivity was detected in corneal Nerve fibers and primary afferent neurons of the ophthalmic branch of the TG of naive mice. MOR expression was significantly higher in both structures under conditions of inflammatory corneal pain. Topical ocular administration of DAMGO strongly reduced both the mechanical (von Frey) and chemical (capsaicin) corneal hypersensitivity associated with inflammatory ocular pain. Repeated instillations of DAMGO also markedly reversed the elevated spontaneous activity of the Ciliary Nerve and responsiveness of corneal polymodal nociceptors that were observed in mice with corneal pain. Finally, these DAMGO-induced behavioral and electrophysiological responses were totally blunted by the topical application of naloxone methiodide, an opioid receptor antagonist. Overall, these results provide evidence that topical pharmacological MOR activation may constitute a therapeutic target for the treatment of corneal pain and improve corneal Nerve function to alleviate chronic pain

  • TRPM8: A therapeutic target for neuroinflammatory symptoms induced by severe dry eye disease
    'MDPI AG', 2020
    Co-Authors: Fakih Darine, Baudouin Christophe, Annabelle Réaux Le Goazigo, Parsadaniantz, Stéphane Mélik
    Abstract:

    International audienceDry eye disease (DED) is commonly associated with ocular surface inflammation and pain. In this study, we evaluated the effectiveness of repeated instillations of transient receptor potential melastatin 8 (TRPM8) ion channel antagonist M8-B on a mouse model of severe DED induced by the excision of extra-orbital lacrimal and Harderian glands. M8-B was topically administered twice a day from day 7 until day 21 after surgery. Cold and mechanical corneal sensitivities and spontaneous ocular pain were monitored at day 21. Ongoing and cold-evoked Ciliary Nerve activities were next evaluated by electrophysiological multi-unit extracellular recording. Corneal inflammation and expression of genes related to neuropathic pain and inflammation were assessed in the trigeminal ganglion. We found that DED mice developed a cold allodynia consistent with higher TRPM8 mRNA expression in the trigeminal ganglion (TG). Chronic M8-B instillations markedly reversed both the corneal mechanical allodynia and spontaneous ocular pain commonly associated with persistent DED. M8-B instillations also diminished the sustained spontaneous and cold-evoked Ciliary Nerve activities observed in DED mice as well as inflammation in the cornea and TG. Overall, our study provides new insight into the effectiveness of TRPM8 blockade for alleviating corneal pain syndrome associated with severe DED, opening a new avenue for ocular pain management

  • Topical treatment with a mu opioid receptor agonist alleviates corneal allodynia and corneal Nerve sensitization in mice
    'Elsevier BV', 2020
    Co-Authors: Joubert Fanny, Guerrero-moreno Adrian, Fakih Darine, Reboussin Elodie, Gaveriaux-ruff Claire, Gallar Juana, Bodineau Laurence, Acosta M. Carmen, Sahel José-alain, Baudouin Christophe
    Abstract:

    Corneal pain is considered to be a core symptom of ocular surface disruption and inflammation. The management of this debilitating condition is still a therapeutic challenge. Recent evidence supports a role of the opioid system in the management of corneal nociception. However, the functional involvement of the mu opioid receptor (MOR) underlying this analgesic effect is not known. We first investigated the expression of the MOR in corneal Nerve fibers and trigeminal ganglion (TG) neurons in control mice and a mouse model of corneal inflammatory pain. We then evaluated the anti-nociceptive and electrophysiological effects of DAMGO ([D-Ala2,N-Me-Phe4,Gly5-ol] enkephalin), a MOR-selective ligand. MOR immunoreactivity was detected in corneal Nerve fibers and primary afferent neurons of the ophthalmic branch of the TG of naive mice. MOR expression was significantly higher in both structures under conditions of inflammatory corneal pain. Topical ocular administration of DAMGO strongly reduced both the mechanical (von Frey) and chemical (capsaicin) corneal hypersensitivity associated with inflammatory ocular pain. Repeated instillations of DAMGO also markedly reversed the elevated spontaneous activity of the Ciliary Nerve and responsiveness of corneal polymodal nociceptors that were observed in mice with corneal pain. Finally, these DAMGO-induced behavioral and electrophysiological responses were totally blunted by the topical application of naloxone methiodide, an opioid receptor antagonist. Overall, these results provide evidence that topical pharmacological MOR activation may constitute a therapeutic target for the treatment of corneal pain and improve corneal Nerve function to alleviate chronic pain.This work was supported by the Sorbonne Université and the Institut National de la Santé et de la Recherche Médicale, the ANR, LabEx LIFESENSES (ANR-10-LABX-65), and IHU FOReSIGHT (ANR-18-IAHU-01). Fanny Joubert was supported by a Fondation de France post-doc fellowship grant. Adrian Guerrero Moreno was funded by a H2020-MSCA-ETN program (IT-DED3) (Grant Agreement 765608).Peer reviewe

  • Effects of corneal injury on Ciliary Nerve fibre activity and corneal nociception in mice: A behavioural and electrophysiological study
    'Wiley', 2019
    Co-Authors: Joubert Fanny, Fakih Darine, Gallar Juana, Bodineau Laurence, Baudouin Christophe, Acosta M. Carmen, Sahel José-alain, Mélik Parsadaniantz Stéphane, Réaux-le Goazigo Annabelle
    Abstract:

    [Background]: Ocular surface diseases are among the most frequent ocular pathologies. Ocular pain following corneal injury is frequently observed in clinic. Corneal sensory innervation is supplied by Ciliary Nerves derived from ophthalmic division of the trigeminal ganglion.[Methods & Results]: Extracellular activity of the mouse Ciliary Nerve was first used to investigate the corneal responsiveness to chemical, mechanical and thermal stimulations in order to specifically study the responses of polymodal nociceptors, mechano‐nociceptors and cold thermoreceptor in a control cornea. Then, in two models of corneal injury (repeated instillations of 0.02% benzalkonium chloride and corneal scraping), we first measured the corneal sensitivity to chemical (eye‐wiping test) and mechanical (von Frey filaments) stimulation. Thereafter, we evaluated whether these corneal injuries modified the spontaneous and chemical stimulation‐evoked activity of the Ciliary Nerve. Both models of injury induced a significant corneal chemical hypersensitivity correlated with an increase of the spontaneous activity of the Ciliary Nerve and a faster response of the Ciliary Nerve after a chemical stimulation.[Conclusions]: Overall, this study provides new insights into the functional aspects of corneal Nerve fibre activity in mice after corneal injury. The increase in Ciliary Nerve activity may thus contribute to the development of ocular pain after corneal damage.[Significance]: This study highlights the parallel increase in Ciliary Nerve activity and corneal sensitivity after corneal injury in mice. The strategy of combining ex vivo electrophysiological recordings of the Ciliary Nerve in mice and corneal sensitivity measurements therefore helps to uncover the functional aspects of corneal pain.This work was supported by Sorbonne Université and the Institut National de la Santé et de la Recherche Médicale, LABEX (LBX3‐OPANI). FJ received a Fondation de France post‐doc fellowship grant.Peer reviewe

Suguru Miyagawa - One of the best experts on this subject based on the ideXlab platform.

  • Asymmetric Wavefront Aberrations and Pupillary Shapes Induced by Electrical Stimulation of Ciliary Nerve in Cats Measured with Compact Wavefront Aberrometer
    2016
    Co-Authors: Suguru Miyagawa, Tomomitsu Miyoshi, Takeshi Morimoto, Yoko Hirohara, Toshifumi Mihashi, Takao Endo, Hiroyuki K, Takashi Fujikado
    Abstract:

    To investigate the changes in the wavefront aberrations and pupillary shape in response to electrical stimulation of the branches of the Ciliary Nerves in cats. Seven eyes of seven cats were studied under general anesthesia. Trains of monophasic pulses (current, 0.1 to 1.0 mA; duration, 0.5 ms/phase; frequency, 5 to 40 Hz) were applied to the lateral or medial branch of the short Ciliary Nerve near the posterior pole of the eye. A pair of electrodes was hooked onto one or both branch of the short Ciliary Nerve. The electrodes were placed about 5 mm from the scleral surface. The wavefront aberrations were recorded continuously for 2 seconds before, 8 seconds during, and for 20 seconds after the electrical stimulation. The pupillary images were simultaneously recorded during the stimulation period. Both the wavefront aberrations and the pupillary images were obtained 10 times/sec with a custom-built wavefront aberrometer. The maximum accommodative amplitude was 1.19 diopters (D) produced by electrical stimulation of the short Ciliary Nerves. The latency of the accommodative changes was very short, and the accommodative level gradually increased up to 4 seconds and reached a plateau. When only one branch of the Ciliary Nerve was stimulated, the pupil dilated asymmetrically, and the oblique astigmatism and one of the asymmetrical wavefront terms was also altered. Our results showed that the wavefront aberrations and pupillary dilations can be measured simultaneously and serially with a compact wavefront aberrometer. The asymmetric pupil dilation and asymmetric changes of the wavefront aberrations suggest that each branch of the ciliar

  • Asymmetric wavefront aberrations and pupillary shapes induced by electrical stimulation of Ciliary Nerve in cats measured with compact wavefront aberrometer.
    PLOS ONE, 2014
    Co-Authors: Suguru Miyagawa, Hiroyuki Kanda, Tomomitsu Miyoshi, Takeshi Morimoto, Yoko Hirohara, Toshifumi Mihashi, Takao Endo, Takashi Fujikado
    Abstract:

    : To investigate the changes in the wavefront aberrations and pupillary shape in response to electrical stimulation of the branches of the Ciliary Nerves in cats. Seven eyes of seven cats were studied under general anesthesia. Trains of monophasic pulses (current, 0.1 to 1.0 mA; duration, 0.5 ms/phase; frequency, 5 to 40 Hz) were applied to the lateral or medial branch of the short Ciliary Nerve near the posterior pole of the eye. A pair of electrodes was hooked onto one or both branch of the short Ciliary Nerve. The electrodes were placed about 5 mm from the scleral surface. The wavefront aberrations were recorded continuously for 2 seconds before, 8 seconds during, and for 20 seconds after the electrical stimulation. The pupillary images were simultaneously recorded during the stimulation period. Both the wavefront aberrations and the pupillary images were obtained 10 times/sec with a custom-built wavefront aberrometer. The maximum accommodative amplitude was 1.19 diopters (D) produced by electrical stimulation of the short Ciliary Nerves. The latency of the accommodative changes was very short, and the accommodative level gradually increased up to 4 seconds and reached a plateau. When only one branch of the Ciliary Nerve was stimulated, the pupil dilated asymmetrically, and the oblique astigmatism and one of the asymmetrical wavefront terms was also altered. Our results showed that the wavefront aberrations and pupillary dilations can be measured simultaneously and serially with a compact wavefront aberrometer. The asymmetric pupil dilation and asymmetric changes of the wavefront aberrations suggest that each branch of the Ciliary Nerve innervates specific segments of the Ciliary muscle and dilator muscle of the pupil.

Aslihan Turhan - One of the best experts on this subject based on the ideXlab platform.

  • Bilateral Nerve Alterations in a Unilateral Experimental Neurotrophic Keratopathy Model: A Lateral Conjunctival Approach for Trigeminal Axotomy
    2016
    Co-Authors: Takefumi Yamaguchi, Aslihan Turhan, Deshea L Harris, Ulrich Von, Andrian Pedram Hamrah
    Abstract:

    To study bilateral Nerve changes in a newly developed novel mouse model for neurotrophic keratopathy by approaching the trigeminal Nerve from the lateral fornix. Surgical axotomy of the Ciliary Nerve of the trigeminal Nerve was performed in adult BALB/c mice at the posterior sclera. Axotomized, contralateral, and sham-treated corneas were excised on post-operative days 1, 3, 5, 7 and 14 and immunofluorescence histochemistry was performed with anti-b-tubulin antibody to evaluate corneal Nerve density. Blink reflex was evaluated using a nylon thread. The survival rate was 100 % with minimal bleeding during axotomy and a surgical time of 860.5 minutes. The blink reflex was diminished at day 1 after axotomy, but remained intact in the contralateral eyes in all mice. The central and peripheral subbasal Nerves were not detectable in the axotomized cornea at day 1 (p,0.001), compared to normal eyes (101.3614.8 and 69.7612.0 mm/mm2 centrally and peripherally). Interestingly, the subbasal Nerve density in the contralateral non-surgical eyes also decreased significantly to 62.462.8 mm/mm2 in the center from day 1 (p,0.001), but did not change in the periphery (77.3611.7 mm/mm2, P = 0.819). Our novel trigeminal axotomy mouse model is highly effective, less invasive, rapid, and has a high survival rate, demonstrating immediate loss of subbasal Nerves in axotomized eyes and decreased subbasal Nerves in contralateral eyes after unilateral axotomy. This model will allow investigating the effects of corneal Nerve damage and serves as a new mode

  • bilateral Nerve alterations in a unilateral experimental neurotrophic keratopathy model a lateral conjunctival approach for trigeminal axotomy
    PLOS ONE, 2013
    Co-Authors: Takefumi Yamaguchi, Aslihan Turhan, Deshea L Harris, Harald Pruss, Kai Hu, Ulrich H Von Andrian, Pedram Hamrah
    Abstract:

    To study bilateral Nerve changes in a newly developed novel mouse model for neurotrophic keratopathy by approaching the trigeminal Nerve from the lateral fornix. Surgical axotomy of the Ciliary Nerve of the trigeminal Nerve was performed in adult BALB/c mice at the posterior sclera. Axotomized, contralateral, and sham-treated corneas were excised on post-operative days 1, 3, 5, 7 and 14 and immunofluorescence histochemistry was performed with anti-β-tubulin antibody to evaluate corneal Nerve density. Blink reflex was evaluated using a nylon thread. The survival rate was 100% with minimal bleeding during axotomy and a surgical time of 8±0.5 minutes. The blink reflex was diminished at day 1 after axotomy, but remained intact in the contralateral eyes in all mice. The central and peripheral subbasal Nerves were not detectable in the axotomized cornea at day 1 (p<0.001), compared to normal eyes (101.3±14.8 and 69.7±12.0 mm/mm2 centrally and peripherally). Interestingly, the subbasal Nerve density in the contralateral non-surgical eyes also decreased significantly to 62.4±2.8 mm/mm2 in the center from day 1 (p<0.001), but did not change in the periphery (77.3±11.7 mm/mm2, P = 0.819). Our novel trigeminal axotomy mouse model is highly effective, less invasive, rapid, and has a high survival rate, demonstrating immediate loss of subbasal Nerves in axotomized eyes and decreased subbasal Nerves in contralateral eyes after unilateral axotomy. This model will allow investigating the effects of corneal Nerve damage and serves as a new model for neurotrophic keratopathy.