The Experts below are selected from a list of 4041 Experts worldwide ranked by ideXlab platform
Liang Zhu - One of the best experts on this subject based on the ideXlab platform.
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Response surface optimization of ultrasound‐assisted flavonoids extraction from the flower of Citrus Aurantium L. var. amara Engl
IEEE Journal of Solid-state Circuits, 2010Co-Authors: Li Yang, Ya-lan Cao, Jian-guo Jiang, Qing-sheng Lin, Jian Chen, Liang ZhuAbstract:Citrus Aurantium L. var. amara Engl is a member of genus Citrus (Rutaceae) and has been used in Chinese medicine with the effectiveness of digestant and expectorant. Ultrasonic-assisted extraction process for maximum flavonoids from the flower of Citrus Aurantium L. var. amara Engl was investigated by response surface methodology. Through single factor experiment, ranges of the main variables (including ethanol concentration, solid/liquid ratio, extraction time and temperature) affecting the extraction yield of flavonoids were confirmed. Box-Behnken central composite design consisting of 24 experimental runs and 5 replicates at zero point was then applied and a regress model was obtained to predict the optimal extraction yield. The ANOVA indicated that the regression equation fits very well with the actual situation, reflecting the relationship between the extraction yield of flavonoids and extraction conditions. The optimal conditions were as follows: extraction temperature 72.11 degrees C, time 51.89 min, ethanol concentration 51.19% and liquid/solid ratio of 40:10. Under the optimal conditions, the maximum response value of yield (1.88%) was consistent with the experimental value (1.87%), indicating the feasibility and validation of response surface methodology in optimizing the extraction of flavonoids from the flower of Citrus Aurantium L. var. amara Engl.
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response surface optimization of ultrasound assisted flavonoids extraction from the flower of Citrus Aurantium l var amara engl
IEEE Journal of Solid-state Circuits, 2010Co-Authors: Li Yang, Ya-lan Cao, Jian-guo Jiang, Qing-sheng Lin, Jian Chen, Liang ZhuAbstract:Citrus Aurantium L. var. amara Engl is a member of genus Citrus (Rutaceae) and has been used in Chinese medicine with the effectiveness of digestant and expectorant. Ultrasonic-assisted extraction process for maximum flavonoids from the flower of Citrus Aurantium L. var. amara Engl was investigated by response surface methodology. Through single factor experiment, ranges of the main variables (including ethanol concentration, solid/liquid ratio, extraction time and temperature) affecting the extraction yield of flavonoids were confirmed. Box-Behnken central composite design consisting of 24 experimental runs and 5 replicates at zero point was then applied and a regress model was obtained to predict the optimal extraction yield. The ANOVA indicated that the regression equation fits very well with the actual situation, reflecting the relationship between the extraction yield of flavonoids and extraction conditions. The optimal conditions were as follows: extraction temperature 72.11 degrees C, time 51.89 min, ethanol concentration 51.19% and liquid/solid ratio of 40:10. Under the optimal conditions, the maximum response value of yield (1.88%) was consistent with the experimental value (1.87%), indicating the feasibility and validation of response surface methodology in optimizing the extraction of flavonoids from the flower of Citrus Aurantium L. var. amara Engl.
Bianca Souza Bagatela - One of the best experts on this subject based on the ideXlab platform.
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high performance thin layer chromatography desorption electrospray ionization mass spectrometry imaging of the crude extract from the peels of Citrus Aurantium l rutaceae
Rapid Communications in Mass Spectrometry, 2015Co-Authors: Bianca Souza Bagatela, Andrey P. Lopes, Elaine C. Cabral, Fábio Ferreira Perazzo, Demian R. IfaAbstract:Rationale Citrus Aurantium L. is a plant belonging to the Rutaceae family, whose extracts are extensively used in weight management products and as thermogenic agents. Here we present two methodologies to analyse the extracts obtained from the peels of Citrus Aurantium L. that usually require multiple sample preparation and detection steps. Methods Polar compounds of the crude extract from the peels of Citrus Aurantium L. (Rutaceae) were investigated by direct infusion electrospray ionization mass spectrometry (ESI-MS) and high-performance thin-layer chromatography (HPTLC) coupled to desorption electrospray ionization mass spectrometry (DESI-MS). ESI-MS was performed in both positive and negative ion modes. Molecular imaging of the HPTLC plates was used for the direct analysis of the phytocompounds present in the crude extract from the peels of Citrus Aurantium L. by DESI-MS imaging. Results Characteristic mass spectra with many diagnostic ions were obtained from the extract analysis, allowing a fast and reliable identification of these species. Tandem mass spectrometry (MS/MS) was employed to confirm the identity of specific metabolites. Conclusions HPTLC/DESI-MS imaging is a relatively fast, versatile, and efficient technique for natural product analysis, since many more ions are observed than with the direct infusion ESI-MS. The MS/MS technique provided information about the component structures, revealing the presence of important bioactive components. The application of DESI-MS imaging may contribute to the improvement identification and characterization of pharmacologically active compounds in phytochemistry. Copyright © 2015 John Wiley & Sons, Ltd.
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High-performance thin-layer chromatography/desorption electrospray ionization mass spectrometry imaging of the crude extract from the peels of Citrus Aurantium L. (Rutaceae).
Rapid Communications in Mass Spectrometry, 2015Co-Authors: Bianca Souza Bagatela, Andrey P. Lopes, Elaine C. Cabral, Fábio Ferreira Perazzo, Demian R. IfaAbstract:Rationale Citrus Aurantium L. is a plant belonging to the Rutaceae family, whose extracts are extensively used in weight management products and as thermogenic agents. Here we present two methodologies to analyse the extracts obtained from the peels of Citrus Aurantium L. that usually require multiple sample preparation and detection steps. Methods Polar compounds of the crude extract from the peels of Citrus Aurantium L. (Rutaceae) were investigated by direct infusion electrospray ionization mass spectrometry (ESI-MS) and high-performance thin-layer chromatography (HPTLC) coupled to desorption electrospray ionization mass spectrometry (DESI-MS). ESI-MS was performed in both positive and negative ion modes. Molecular imaging of the HPTLC plates was used for the direct analysis of the phytocompounds present in the crude extract from the peels of Citrus Aurantium L. by DESI-MS imaging. Results Characteristic mass spectra with many diagnostic ions were obtained from the extract analysis, allowing a fast and reliable identification of these species. Tandem mass spectrometry (MS/MS) was employed to confirm the identity of specific metabolites. Conclusions HPTLC/DESI-MS imaging is a relatively fast, versatile, and efficient technique for natural product analysis, since many more ions are observed than with the direct infusion ESI-MS. The MS/MS technique provided information about the component structures, revealing the presence of important bioactive components. The application of DESI-MS imaging may contribute to the improvement identification and characterization of pharmacologically active compounds in phytochemistry. Copyright © 2015 John Wiley & Sons, Ltd.
Li Yang - One of the best experts on this subject based on the ideXlab platform.
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Response surface optimization of ultrasound‐assisted flavonoids extraction from the flower of Citrus Aurantium L. var. amara Engl
IEEE Journal of Solid-state Circuits, 2010Co-Authors: Li Yang, Ya-lan Cao, Jian-guo Jiang, Qing-sheng Lin, Jian Chen, Liang ZhuAbstract:Citrus Aurantium L. var. amara Engl is a member of genus Citrus (Rutaceae) and has been used in Chinese medicine with the effectiveness of digestant and expectorant. Ultrasonic-assisted extraction process for maximum flavonoids from the flower of Citrus Aurantium L. var. amara Engl was investigated by response surface methodology. Through single factor experiment, ranges of the main variables (including ethanol concentration, solid/liquid ratio, extraction time and temperature) affecting the extraction yield of flavonoids were confirmed. Box-Behnken central composite design consisting of 24 experimental runs and 5 replicates at zero point was then applied and a regress model was obtained to predict the optimal extraction yield. The ANOVA indicated that the regression equation fits very well with the actual situation, reflecting the relationship between the extraction yield of flavonoids and extraction conditions. The optimal conditions were as follows: extraction temperature 72.11 degrees C, time 51.89 min, ethanol concentration 51.19% and liquid/solid ratio of 40:10. Under the optimal conditions, the maximum response value of yield (1.88%) was consistent with the experimental value (1.87%), indicating the feasibility and validation of response surface methodology in optimizing the extraction of flavonoids from the flower of Citrus Aurantium L. var. amara Engl.
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response surface optimization of ultrasound assisted flavonoids extraction from the flower of Citrus Aurantium l var amara engl
IEEE Journal of Solid-state Circuits, 2010Co-Authors: Li Yang, Ya-lan Cao, Jian-guo Jiang, Qing-sheng Lin, Jian Chen, Liang ZhuAbstract:Citrus Aurantium L. var. amara Engl is a member of genus Citrus (Rutaceae) and has been used in Chinese medicine with the effectiveness of digestant and expectorant. Ultrasonic-assisted extraction process for maximum flavonoids from the flower of Citrus Aurantium L. var. amara Engl was investigated by response surface methodology. Through single factor experiment, ranges of the main variables (including ethanol concentration, solid/liquid ratio, extraction time and temperature) affecting the extraction yield of flavonoids were confirmed. Box-Behnken central composite design consisting of 24 experimental runs and 5 replicates at zero point was then applied and a regress model was obtained to predict the optimal extraction yield. The ANOVA indicated that the regression equation fits very well with the actual situation, reflecting the relationship between the extraction yield of flavonoids and extraction conditions. The optimal conditions were as follows: extraction temperature 72.11 degrees C, time 51.89 min, ethanol concentration 51.19% and liquid/solid ratio of 40:10. Under the optimal conditions, the maximum response value of yield (1.88%) was consistent with the experimental value (1.87%), indicating the feasibility and validation of response surface methodology in optimizing the extraction of flavonoids from the flower of Citrus Aurantium L. var. amara Engl.
Jian-guo Jiang - One of the best experts on this subject based on the ideXlab platform.
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Extraction optimization and adsorption isotherm kinetics of polyphenols from blossoms of Citrus Aurantium L. var. amara Engl
Separation Science and Technology, 2019Co-Authors: Chun-yan Shen, Tian-xing Wang, Jian-guo JiangAbstract:ABSTRACTCrude extracts were extracted from blossoms of Citrus Aurantium L. var. amara Engl. with 50% ethanol (CAVAP-E), 50% methanol (CAVAP-M), and hot water (CAVAP-W). CAVAP-W had the highest poly...
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Response surface optimization of ultrasound‐assisted flavonoids extraction from the flower of Citrus Aurantium L. var. amara Engl
IEEE Journal of Solid-state Circuits, 2010Co-Authors: Li Yang, Ya-lan Cao, Jian-guo Jiang, Qing-sheng Lin, Jian Chen, Liang ZhuAbstract:Citrus Aurantium L. var. amara Engl is a member of genus Citrus (Rutaceae) and has been used in Chinese medicine with the effectiveness of digestant and expectorant. Ultrasonic-assisted extraction process for maximum flavonoids from the flower of Citrus Aurantium L. var. amara Engl was investigated by response surface methodology. Through single factor experiment, ranges of the main variables (including ethanol concentration, solid/liquid ratio, extraction time and temperature) affecting the extraction yield of flavonoids were confirmed. Box-Behnken central composite design consisting of 24 experimental runs and 5 replicates at zero point was then applied and a regress model was obtained to predict the optimal extraction yield. The ANOVA indicated that the regression equation fits very well with the actual situation, reflecting the relationship between the extraction yield of flavonoids and extraction conditions. The optimal conditions were as follows: extraction temperature 72.11 degrees C, time 51.89 min, ethanol concentration 51.19% and liquid/solid ratio of 40:10. Under the optimal conditions, the maximum response value of yield (1.88%) was consistent with the experimental value (1.87%), indicating the feasibility and validation of response surface methodology in optimizing the extraction of flavonoids from the flower of Citrus Aurantium L. var. amara Engl.
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response surface optimization of ultrasound assisted flavonoids extraction from the flower of Citrus Aurantium l var amara engl
IEEE Journal of Solid-state Circuits, 2010Co-Authors: Li Yang, Ya-lan Cao, Jian-guo Jiang, Qing-sheng Lin, Jian Chen, Liang ZhuAbstract:Citrus Aurantium L. var. amara Engl is a member of genus Citrus (Rutaceae) and has been used in Chinese medicine with the effectiveness of digestant and expectorant. Ultrasonic-assisted extraction process for maximum flavonoids from the flower of Citrus Aurantium L. var. amara Engl was investigated by response surface methodology. Through single factor experiment, ranges of the main variables (including ethanol concentration, solid/liquid ratio, extraction time and temperature) affecting the extraction yield of flavonoids were confirmed. Box-Behnken central composite design consisting of 24 experimental runs and 5 replicates at zero point was then applied and a regress model was obtained to predict the optimal extraction yield. The ANOVA indicated that the regression equation fits very well with the actual situation, reflecting the relationship between the extraction yield of flavonoids and extraction conditions. The optimal conditions were as follows: extraction temperature 72.11 degrees C, time 51.89 min, ethanol concentration 51.19% and liquid/solid ratio of 40:10. Under the optimal conditions, the maximum response value of yield (1.88%) was consistent with the experimental value (1.87%), indicating the feasibility and validation of response surface methodology in optimizing the extraction of flavonoids from the flower of Citrus Aurantium L. var. amara Engl.
Demian R. Ifa - One of the best experts on this subject based on the ideXlab platform.
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high performance thin layer chromatography desorption electrospray ionization mass spectrometry imaging of the crude extract from the peels of Citrus Aurantium l rutaceae
Rapid Communications in Mass Spectrometry, 2015Co-Authors: Bianca Souza Bagatela, Andrey P. Lopes, Elaine C. Cabral, Fábio Ferreira Perazzo, Demian R. IfaAbstract:Rationale Citrus Aurantium L. is a plant belonging to the Rutaceae family, whose extracts are extensively used in weight management products and as thermogenic agents. Here we present two methodologies to analyse the extracts obtained from the peels of Citrus Aurantium L. that usually require multiple sample preparation and detection steps. Methods Polar compounds of the crude extract from the peels of Citrus Aurantium L. (Rutaceae) were investigated by direct infusion electrospray ionization mass spectrometry (ESI-MS) and high-performance thin-layer chromatography (HPTLC) coupled to desorption electrospray ionization mass spectrometry (DESI-MS). ESI-MS was performed in both positive and negative ion modes. Molecular imaging of the HPTLC plates was used for the direct analysis of the phytocompounds present in the crude extract from the peels of Citrus Aurantium L. by DESI-MS imaging. Results Characteristic mass spectra with many diagnostic ions were obtained from the extract analysis, allowing a fast and reliable identification of these species. Tandem mass spectrometry (MS/MS) was employed to confirm the identity of specific metabolites. Conclusions HPTLC/DESI-MS imaging is a relatively fast, versatile, and efficient technique for natural product analysis, since many more ions are observed than with the direct infusion ESI-MS. The MS/MS technique provided information about the component structures, revealing the presence of important bioactive components. The application of DESI-MS imaging may contribute to the improvement identification and characterization of pharmacologically active compounds in phytochemistry. Copyright © 2015 John Wiley & Sons, Ltd.
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High-performance thin-layer chromatography/desorption electrospray ionization mass spectrometry imaging of the crude extract from the peels of Citrus Aurantium L. (Rutaceae).
Rapid Communications in Mass Spectrometry, 2015Co-Authors: Bianca Souza Bagatela, Andrey P. Lopes, Elaine C. Cabral, Fábio Ferreira Perazzo, Demian R. IfaAbstract:Rationale Citrus Aurantium L. is a plant belonging to the Rutaceae family, whose extracts are extensively used in weight management products and as thermogenic agents. Here we present two methodologies to analyse the extracts obtained from the peels of Citrus Aurantium L. that usually require multiple sample preparation and detection steps. Methods Polar compounds of the crude extract from the peels of Citrus Aurantium L. (Rutaceae) were investigated by direct infusion electrospray ionization mass spectrometry (ESI-MS) and high-performance thin-layer chromatography (HPTLC) coupled to desorption electrospray ionization mass spectrometry (DESI-MS). ESI-MS was performed in both positive and negative ion modes. Molecular imaging of the HPTLC plates was used for the direct analysis of the phytocompounds present in the crude extract from the peels of Citrus Aurantium L. by DESI-MS imaging. Results Characteristic mass spectra with many diagnostic ions were obtained from the extract analysis, allowing a fast and reliable identification of these species. Tandem mass spectrometry (MS/MS) was employed to confirm the identity of specific metabolites. Conclusions HPTLC/DESI-MS imaging is a relatively fast, versatile, and efficient technique for natural product analysis, since many more ions are observed than with the direct infusion ESI-MS. The MS/MS technique provided information about the component structures, revealing the presence of important bioactive components. The application of DESI-MS imaging may contribute to the improvement identification and characterization of pharmacologically active compounds in phytochemistry. Copyright © 2015 John Wiley & Sons, Ltd.