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He Hong - One of the best experts on this subject based on the ideXlab platform.

  • Vitrified cryopreservation and regeneration of shoot-tips for Citrus Medica var.sarcodactylis
    Chinese Traditional and Herbal Drugs, 2009
    Co-Authors: He Hong
    Abstract:

    Objective To provide a new approach of preservation for germplasm resources of Citrus Medica var.sarcodactylis(CMS).Methods A micrografting test was made on CMS shoot-tips after vitrified cryopreservation,resulting in living shoot-tip.Results First the CMS shoot-tip is inoculated in medium consisting of MS,5%DMSO,and 5%sucrose for 48 h preincubation.Then the shoot-tips were cut off 3—4 mm long and fore-treated by 60%PVS_2 at room temperature(25℃) for 30 min.After that,they were treated by PVS_2 at 0℃for another 80 min and conserved in liquid nitrogen for 24 h.Next the shoot-tips were defrosted by aqueous bath at 40℃and cleaned twice at room temperature(25℃) by MT minimal medium with additive 1.2 mol/L sucrose,10 min once.Finally data collected recorded a higher survival rate of 81.25%by TTC inspectation and a better regeneration rate of 52.94%if the CMS shoot-tips had been grafted on darkly-cultured parental stock,growing,and differentiating normally afterwards.The plantlets detected by polymerase chain reaction(PCR) showed that the pathogens of Huanglong disease had been removed.Conclusion Therefore a conclusion is made that the approach of vitrified cryopreservation may be applied to the preservation of CMS germplasm resources.

  • An RAPD Analysis on Genetic Diversity of Citrus Medica L. var. sarcodactylis(Noot.) Swingle Germplasms
    Lishizhen Medicine and Materia Medica Research, 2009
    Co-Authors: He Hong
    Abstract:

    ObjectiveTo study the diversity and heredity relationship of Citrus Medica L.var.sarcodactylis(Noot.) Swingle(CMS)'s genetic resource.MethodsGenetic relationship of 13 CMS germplasm materials was analyzed with RAPD.ResultsThe RAPD amplification of 13 materials by 16 kinds of primers selected from 110 ones resulted in 185 electrophoresis strips with 168 strips(90.8%) polymorphic.3 groups were divided among the testing materials at the similarity coefficient value of 0.67 via cluster analysis.ConclusionAffluent genetic diversities exist among the CMS germplasms and the clustered results correspond respectively to the classification of 3 major CMS varieties according to the geographic settings.

  • Most Diseases and Their Control on Citrus Medica L. var sarcodactylis (Noot.) Swingle
    Research and Practice on Chinese Medicines, 2003
    Co-Authors: He Hong
    Abstract:

    Objective To establish the general control measures against diseases of Citrus Medica L. var sarcodactylis (Noot.) Swingle , in order to reduce their harm and accelerate the production.Methods Literature search and field investigation were used.Results The status and control measures of such diseases as tristeza,huanglongbing,authracnose and cankerous disease were summarized,and the reasons and spread fashions of them were analyzed.Moreover, the general control measures were established.Conclusion To control them effectively, prevention is most important, and agricultural methods,biologic methods and chemical germicides should be used together.

Sl Munne - One of the best experts on this subject based on the ideXlab platform.

  • Free Radical Scavenging Activity of Gamma Irradiated and Unirradiated Citrus Medica Empty Juice Sacs
    Asian Journal of Research in Chemistry, 2011
    Co-Authors: Sl Munne, D. V. Parwate, V. N. Ingle, D.s. Panchbhai, V.s. Nagpurkar
    Abstract:

    Antioxidant activity of methanol extract of gamma irradiated and unirradiated Citrus Medica fruit bio-mass was investigated for its free radical scavenging activity. Antioxidant activity of methanol extract was evaluated by DPPH and nitric oxide radical scavenging method. In DPPH method 100 μg/ml of MEUCM and MERCM exhibited 58.14% and 55.95% inhibition and in Nitric oxide method 100 μg/ml concentration of MEUCM and MERCM shows 58.28%, 50.32% inhibition respectively.

  • Antihelmintic Activity of Methanol Extract of Gamma Irradiated and Unirradiated Citrus Medica Fruit Bio-Mass
    Research Journal of Pharmacy and Technology, 2011
    Co-Authors: Sl Munne, D. V. Parwate, V. N. Ingle, Ma Kamble
    Abstract:

    The present study was undertaken to evaluate anthelmintic activity of methanolic extract of irradiated and unirradiated Citrus Medica fruit bio-mass belonging to Rutaceae family using Pheretima posthuma as test worms. Various concentrations (10–50 mg/ml) of methanolic extracts were tested in the bioassay, which involved determination of time of paralysis (P) and time of death (D) of the worms. Piperazine citrate was taken as standard reference and distilled water as control. The results of present study indicated that the unirradiated extract significantly demonstrated paralysis and death of worms in less time as compare to radiated sample at higher concentration of 50 mg/ml.

V.s. Nagpurkar - One of the best experts on this subject based on the ideXlab platform.

D. V. Parwate - One of the best experts on this subject based on the ideXlab platform.

  • Free Radical Scavenging Activity of Gamma Irradiated and Unirradiated Citrus Medica Empty Juice Sacs
    Asian Journal of Research in Chemistry, 2011
    Co-Authors: Sl Munne, D. V. Parwate, V. N. Ingle, D.s. Panchbhai, V.s. Nagpurkar
    Abstract:

    Antioxidant activity of methanol extract of gamma irradiated and unirradiated Citrus Medica fruit bio-mass was investigated for its free radical scavenging activity. Antioxidant activity of methanol extract was evaluated by DPPH and nitric oxide radical scavenging method. In DPPH method 100 μg/ml of MEUCM and MERCM exhibited 58.14% and 55.95% inhibition and in Nitric oxide method 100 μg/ml concentration of MEUCM and MERCM shows 58.28%, 50.32% inhibition respectively.

  • Antihelmintic Activity of Methanol Extract of Gamma Irradiated and Unirradiated Citrus Medica Fruit Bio-Mass
    Research Journal of Pharmacy and Technology, 2011
    Co-Authors: Sl Munne, D. V. Parwate, V. N. Ingle, Ma Kamble
    Abstract:

    The present study was undertaken to evaluate anthelmintic activity of methanolic extract of irradiated and unirradiated Citrus Medica fruit bio-mass belonging to Rutaceae family using Pheretima posthuma as test worms. Various concentrations (10–50 mg/ml) of methanolic extracts were tested in the bioassay, which involved determination of time of paralysis (P) and time of death (D) of the worms. Piperazine citrate was taken as standard reference and distilled water as control. The results of present study indicated that the unirradiated extract significantly demonstrated paralysis and death of worms in less time as compare to radiated sample at higher concentration of 50 mg/ml.

V. N. Ingle - One of the best experts on this subject based on the ideXlab platform.

  • Free Radical Scavenging Activity of Gamma Irradiated and Unirradiated Citrus Medica Empty Juice Sacs
    Asian Journal of Research in Chemistry, 2011
    Co-Authors: Sl Munne, D. V. Parwate, V. N. Ingle, D.s. Panchbhai, V.s. Nagpurkar
    Abstract:

    Antioxidant activity of methanol extract of gamma irradiated and unirradiated Citrus Medica fruit bio-mass was investigated for its free radical scavenging activity. Antioxidant activity of methanol extract was evaluated by DPPH and nitric oxide radical scavenging method. In DPPH method 100 μg/ml of MEUCM and MERCM exhibited 58.14% and 55.95% inhibition and in Nitric oxide method 100 μg/ml concentration of MEUCM and MERCM shows 58.28%, 50.32% inhibition respectively.

  • Antihelmintic Activity of Methanol Extract of Gamma Irradiated and Unirradiated Citrus Medica Fruit Bio-Mass
    Research Journal of Pharmacy and Technology, 2011
    Co-Authors: Sl Munne, D. V. Parwate, V. N. Ingle, Ma Kamble
    Abstract:

    The present study was undertaken to evaluate anthelmintic activity of methanolic extract of irradiated and unirradiated Citrus Medica fruit bio-mass belonging to Rutaceae family using Pheretima posthuma as test worms. Various concentrations (10–50 mg/ml) of methanolic extracts were tested in the bioassay, which involved determination of time of paralysis (P) and time of death (D) of the worms. Piperazine citrate was taken as standard reference and distilled water as control. The results of present study indicated that the unirradiated extract significantly demonstrated paralysis and death of worms in less time as compare to radiated sample at higher concentration of 50 mg/ml.