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Julian Rafael Dib - One of the best experts on this subject based on the ideXlab platform.
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protection of citrus fruits from postharvest infection with penicillium digitatum and degradation of patulin by biocontrol yeast Clavispora lusitaniae 146
Microorganisms, 2020Co-Authors: Mariana Andrea Diaz, Maria Florencia Perez, Martina Maria Pereyra, Josefina Maria Cordoba, Fabricio Fabian Soliz Santander, Mohammad Alhussein, Petr Karlovsky, Julian Rafael DibAbstract:Fil: Diaz, Mariana Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
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effect of stress factors associated with postharvest citrus conditions on the viability and biocontrol activity of Clavispora lusitaniae strain 146
PLOS ONE, 2020Co-Authors: Martina Maria Pereyra, Mariana Andrea Diaz, Julian Rafael Dib, Friedhelm MeinhardtAbstract:Only quite recently, we have shown that yeast strains Clavispora lusitaniae 146 and Pichia fermentans 27 can act as efficient biocontrol agents for combating postharvest fungal diseases in lemons. During postharvest and storage conditions, microorganisms are subject to different stress factors that could affect both their survival and their protective capacity. Understanding the tolerance of yeasts to environmental stress factors could support the future development and commercial application of biological control formulations based on such organisms. Thus, the impact of different stressors on the viability and protection efficiency of C. lusitaniae strain 146 and P. fermentans strain 27 was evaluated, and the yeasts were subjected to oxidative stress, thermal treatments, exposure to NaOCl, osmotic stress, and ultraviolet irradiation. Candida oleophila strain O served as the reference control. C. lusitaniae 146 was more resistant to H2O2 in plate assays; however, in liquid media there was no significant difference to the other strains. Strain 146 was less affected by NaOCl, being able to survive with 300 ppm. P. fermentans 27 was the strain most heavily affected by osmotic pressure, while strains 146 and strain O showed a similar adaptation. UV-B irradiation severely affected C. oleophila and P. fermentans, while C. lusitaniae was the most resistant. Strains 146 and 27 were similarly tolerant to thermal shocks, compared to the reference strain, which was less viable. In in vivo tests, exposure to 10 mM H2O2, 45°C or 200 ppm NaOCl prior to fruit inoculation, reduced the antagonistic activity against the pathogen Penicillium digitatum. However, in no case was the biocontrol efficiency reduced to less than 50%. As C. lusitaniae 146 demonstrated a great potential to combat P. digitatum under a wide range of conditions, the organism is a promising candidate as an effective and valuable alternative to toxic fungicides.
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biocontrol features of Clavispora lusitaniae against penicillium digitatum on lemons
Postharvest Biology and Technology, 2019Co-Authors: Maria Florencia Perez, Mariana Andrea Diaz, Martina Maria Pereyra, Josefina Maria Cordoba, Ana Sofia Isas, Milena Sepulveda, Jacqueline Ramallo, Julian Rafael DibAbstract:Abstract Penicillium digitatum is the main pathogen which causes postharvest decays in lemons, and the use of synthetic fungicides is the principal way of controlling these postharvest infections. In our previous work, identifying biological alternatives to the use of fungicides, a killer strain of Clavispora lusitaniae 146, which exhibited highly protective activity against green mold on lemons, was isolated from citrus plants. The aim of the present work was to study the ability of C. lusitaniae 146 to colonize wounds in lemon fruit and its effect on P. digitatum spore germination. Additionally, the protection efficacy of yeast strain 146 against an imazalil-resistant P. digitatum as well as yeast survival on commercial waxes were also evaluated. Results indicated that C. lusitaniae 146 exhibited an evident ability to colonize wounds in lemons and continue to grow at both low and room temperatures. In addition, the use of heat-inactivated cells showed a certain degree of efficacy against fruit decays. The ability of C. lusitaniae 146 to inhibit spore germination and its high efficiency against both sensitive and resistant fungi to fungicides were also demonstrated. Lastly, it was found that C. lusitaniae 146 could survive in natural waxes for 7 d at 25 °C and 40 d at 8 °C. Thus, our studies confirmed the potential industrial use of C. lusitaniae 146 as an effective biocontrol agent against postharvest infections of lemons caused by P. digitatum.
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antagonistic yeasts for the biological control of penicillium digitatum on lemons stored under export conditions
Biological Control, 2017Co-Authors: Maria Florencia Perez, Ana Sofia Isas, Milena Sepulveda, Jacqueline Ramallo, Julia Perez Ibarreche, Julian Rafael DibAbstract:Abstract The main phytopathogen that affects lemons in the postharvest stage is Penicillium digitatum. Currently, chemical fungicides which have been shown to have negative impacts on both, the environment and human health are used to combat fungus decay. As alternatives to the use of these fungicides killer yeasts which were previously isolated from citrus plants, showed an effective biocontrol effect against Penicillium spp. In order to determine the efficacy of selected killer yeasts throughout the harvest period, in vivo protection trials were performed at both room and low temperature; these trials were carried out under similar conditions to those applied for export to overseas markets. In addition, tolerance to commonly used postharvest fungicides was also tested. Clavispora lusitaniae 146 and Pichia fermentans 27 showed high and consistent protection during the whole harvest period in both temperature conditions. Even their protection efficiencies were superior to a commercial product based on Candida oleophila. Strains 146 and 27 were tolerant to fungicides; therefore; combined application of chemical agents along with the biocontrol agent could be employed in postharvest stages.
Eric Dannaoui - One of the best experts on this subject based on the ideXlab platform.
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Method-dependent epidemiological cutoff values (ECVs) for detection of triazole resistance in Candida and Aspergillus species for the SYO colorimetric broth and Etest agar diffusion methods
'American Society for Microbiology', 2019Co-Authors: Ana Espinel-ingroff, J. Turnidge, Ana Alastruey-izquierdo, F. Botterel, Emilia Cantón, C. Castro, Y. Chen, Erja Chryssanthou, Eric DannaouiAbstract:Although the Sensitrite Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method-agent-dependent): 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrata species complex (SC), 157 C. (Meyerozyma) guilliermondii, 676 C. krusei (Pichia kudriavzevii), 298 C (Clavispora) lusitaniae, 911 and 3,691 C. parapsilosissensu stricto (SS) and C. parapsilosisSC, respectively, 36 C. metapsilosis, 110 C. orthopsilosis, 1,854 C. tropicalis, 244 Saccharomyces cerevisiae, 1,409 Aspergillus fumigatus, 389 A. flavus, 130 A. nidulans, 233 A. niger, and 302 A. terreus complexes. SYO/Etest MICs for 282 confirmed non-WT isolates were included: ERG11 (C. albicans), ERG11 and MRR1 (C. parapsilosis), cyp51A (A. fumigatus), and CDR2, CDR1 overexpression (C. albicans and C. glabrata, respectively). Interlaboratory modal agreement was superior by SYO for yeast spp., and by the Etest for Aspergillus spp. Distributions fulfilling CLSI criteria for ECV definition were pooled and we proposed SYO ECVs for S. cerevisiae, 9 yeast and 3 Aspergillus species, and Etest ECVs for 5 yeast and 4 Aspergillus species. The posaconazole SYO ECV of 0.06 µg/ml for C. albicans and the Etest itraconazole ECV of 2 µg/ml for A. fumigatus were the best predictors of non-WT isolates. These findings support the need for method-dependent ECVs, as overall, the SYO appears to perform better for susceptibility testing of yeast spp. and the Etest for Aspergillus spp. Further evaluations should be conducted with more Candida mutants
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method dependent epidemiological cutoff values for detection of triazole resistance in candida and aspergillus species for the sensititre yeastone colorimetric broth and etest agar diffusion methods
Antimicrobial Agents and Chemotherapy, 2018Co-Authors: Ana Espinelingroff, J. Turnidge, Erja Chryssanthou, Ana Alastrueyizquierdo, Francoise Otterel, E Canto, Carmen Barroso Castro, Yeechu Che, Eric DannaouiAbstract:: Although the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrata species complex, 157 C.guilliermondii (Meyerozyma guilliermondii), 676 C. krusei (Pichia kudriavzevii), 298 C.lusitaniae (Clavispora lusitaniae), 911 C.parapsilosissensu stricto, 3,691 C.parapsilosis species complex, 36 C.metapsilosis, 110 C.orthopsilosis, 1,854 C.tropicalis, 244 Saccharomyces cerevisiae, 1,409 Aspergillus fumigatus, 389 A.flavus, 130 A.nidulans, 233 A.niger, and 302 A.terreus complex isolates. SYO/Etest MICs for 282 confirmed non-wild-type (non-WT) isolates were included: ERG11 (C. albicans), ERG11 and MRR1 (C. parapsilosis), cyp51A (A. fumigatus), and CDR2 and CDR1 overexpression (C. albicans and C. glabrata, respectively). Interlaboratory modal agreement was superior by SYO for yeast species and by the Etest for Aspergillus spp. Distributions fulfilling CLSI criteria for epidemiological cutoff value (ECV) definition were pooled, and we proposed SYO ECVs for S. cerevisiae and 9 yeast and 3 Aspergillus species and Etest ECVs for 5 yeast and 4 Aspergillus species. The posaconazole SYO ECV of 0.06 µg/ml for C. albicans and the Etest itraconazole ECV of 2 µg/ml for A. fumigatus were the best predictors of non-WT isolates. These findings support the need for method-dependent ECVs, as, overall, the SYO appears to perform better for susceptibility testing of yeast species and the Etest appears to perform better for susceptibility testing of Aspergillus spp. Further evaluations should be conducted with more Candida mutants.
Martina Maria Pereyra - One of the best experts on this subject based on the ideXlab platform.
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protection of citrus fruits from postharvest infection with penicillium digitatum and degradation of patulin by biocontrol yeast Clavispora lusitaniae 146
Microorganisms, 2020Co-Authors: Mariana Andrea Diaz, Maria Florencia Perez, Martina Maria Pereyra, Josefina Maria Cordoba, Fabricio Fabian Soliz Santander, Mohammad Alhussein, Petr Karlovsky, Julian Rafael DibAbstract:Fil: Diaz, Mariana Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
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effect of stress factors associated with postharvest citrus conditions on the viability and biocontrol activity of Clavispora lusitaniae strain 146
PLOS ONE, 2020Co-Authors: Martina Maria Pereyra, Mariana Andrea Diaz, Julian Rafael Dib, Friedhelm MeinhardtAbstract:Only quite recently, we have shown that yeast strains Clavispora lusitaniae 146 and Pichia fermentans 27 can act as efficient biocontrol agents for combating postharvest fungal diseases in lemons. During postharvest and storage conditions, microorganisms are subject to different stress factors that could affect both their survival and their protective capacity. Understanding the tolerance of yeasts to environmental stress factors could support the future development and commercial application of biological control formulations based on such organisms. Thus, the impact of different stressors on the viability and protection efficiency of C. lusitaniae strain 146 and P. fermentans strain 27 was evaluated, and the yeasts were subjected to oxidative stress, thermal treatments, exposure to NaOCl, osmotic stress, and ultraviolet irradiation. Candida oleophila strain O served as the reference control. C. lusitaniae 146 was more resistant to H2O2 in plate assays; however, in liquid media there was no significant difference to the other strains. Strain 146 was less affected by NaOCl, being able to survive with 300 ppm. P. fermentans 27 was the strain most heavily affected by osmotic pressure, while strains 146 and strain O showed a similar adaptation. UV-B irradiation severely affected C. oleophila and P. fermentans, while C. lusitaniae was the most resistant. Strains 146 and 27 were similarly tolerant to thermal shocks, compared to the reference strain, which was less viable. In in vivo tests, exposure to 10 mM H2O2, 45°C or 200 ppm NaOCl prior to fruit inoculation, reduced the antagonistic activity against the pathogen Penicillium digitatum. However, in no case was the biocontrol efficiency reduced to less than 50%. As C. lusitaniae 146 demonstrated a great potential to combat P. digitatum under a wide range of conditions, the organism is a promising candidate as an effective and valuable alternative to toxic fungicides.
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biocontrol features of Clavispora lusitaniae against penicillium digitatum on lemons
Postharvest Biology and Technology, 2019Co-Authors: Maria Florencia Perez, Mariana Andrea Diaz, Martina Maria Pereyra, Josefina Maria Cordoba, Ana Sofia Isas, Milena Sepulveda, Jacqueline Ramallo, Julian Rafael DibAbstract:Abstract Penicillium digitatum is the main pathogen which causes postharvest decays in lemons, and the use of synthetic fungicides is the principal way of controlling these postharvest infections. In our previous work, identifying biological alternatives to the use of fungicides, a killer strain of Clavispora lusitaniae 146, which exhibited highly protective activity against green mold on lemons, was isolated from citrus plants. The aim of the present work was to study the ability of C. lusitaniae 146 to colonize wounds in lemon fruit and its effect on P. digitatum spore germination. Additionally, the protection efficacy of yeast strain 146 against an imazalil-resistant P. digitatum as well as yeast survival on commercial waxes were also evaluated. Results indicated that C. lusitaniae 146 exhibited an evident ability to colonize wounds in lemons and continue to grow at both low and room temperatures. In addition, the use of heat-inactivated cells showed a certain degree of efficacy against fruit decays. The ability of C. lusitaniae 146 to inhibit spore germination and its high efficiency against both sensitive and resistant fungi to fungicides were also demonstrated. Lastly, it was found that C. lusitaniae 146 could survive in natural waxes for 7 d at 25 °C and 40 d at 8 °C. Thus, our studies confirmed the potential industrial use of C. lusitaniae 146 as an effective biocontrol agent against postharvest infections of lemons caused by P. digitatum.
Mariana Andrea Diaz - One of the best experts on this subject based on the ideXlab platform.
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protection of citrus fruits from postharvest infection with penicillium digitatum and degradation of patulin by biocontrol yeast Clavispora lusitaniae 146
Microorganisms, 2020Co-Authors: Mariana Andrea Diaz, Maria Florencia Perez, Martina Maria Pereyra, Josefina Maria Cordoba, Fabricio Fabian Soliz Santander, Mohammad Alhussein, Petr Karlovsky, Julian Rafael DibAbstract:Fil: Diaz, Mariana Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
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effect of stress factors associated with postharvest citrus conditions on the viability and biocontrol activity of Clavispora lusitaniae strain 146
PLOS ONE, 2020Co-Authors: Martina Maria Pereyra, Mariana Andrea Diaz, Julian Rafael Dib, Friedhelm MeinhardtAbstract:Only quite recently, we have shown that yeast strains Clavispora lusitaniae 146 and Pichia fermentans 27 can act as efficient biocontrol agents for combating postharvest fungal diseases in lemons. During postharvest and storage conditions, microorganisms are subject to different stress factors that could affect both their survival and their protective capacity. Understanding the tolerance of yeasts to environmental stress factors could support the future development and commercial application of biological control formulations based on such organisms. Thus, the impact of different stressors on the viability and protection efficiency of C. lusitaniae strain 146 and P. fermentans strain 27 was evaluated, and the yeasts were subjected to oxidative stress, thermal treatments, exposure to NaOCl, osmotic stress, and ultraviolet irradiation. Candida oleophila strain O served as the reference control. C. lusitaniae 146 was more resistant to H2O2 in plate assays; however, in liquid media there was no significant difference to the other strains. Strain 146 was less affected by NaOCl, being able to survive with 300 ppm. P. fermentans 27 was the strain most heavily affected by osmotic pressure, while strains 146 and strain O showed a similar adaptation. UV-B irradiation severely affected C. oleophila and P. fermentans, while C. lusitaniae was the most resistant. Strains 146 and 27 were similarly tolerant to thermal shocks, compared to the reference strain, which was less viable. In in vivo tests, exposure to 10 mM H2O2, 45°C or 200 ppm NaOCl prior to fruit inoculation, reduced the antagonistic activity against the pathogen Penicillium digitatum. However, in no case was the biocontrol efficiency reduced to less than 50%. As C. lusitaniae 146 demonstrated a great potential to combat P. digitatum under a wide range of conditions, the organism is a promising candidate as an effective and valuable alternative to toxic fungicides.
-
biocontrol features of Clavispora lusitaniae against penicillium digitatum on lemons
Postharvest Biology and Technology, 2019Co-Authors: Maria Florencia Perez, Mariana Andrea Diaz, Martina Maria Pereyra, Josefina Maria Cordoba, Ana Sofia Isas, Milena Sepulveda, Jacqueline Ramallo, Julian Rafael DibAbstract:Abstract Penicillium digitatum is the main pathogen which causes postharvest decays in lemons, and the use of synthetic fungicides is the principal way of controlling these postharvest infections. In our previous work, identifying biological alternatives to the use of fungicides, a killer strain of Clavispora lusitaniae 146, which exhibited highly protective activity against green mold on lemons, was isolated from citrus plants. The aim of the present work was to study the ability of C. lusitaniae 146 to colonize wounds in lemon fruit and its effect on P. digitatum spore germination. Additionally, the protection efficacy of yeast strain 146 against an imazalil-resistant P. digitatum as well as yeast survival on commercial waxes were also evaluated. Results indicated that C. lusitaniae 146 exhibited an evident ability to colonize wounds in lemons and continue to grow at both low and room temperatures. In addition, the use of heat-inactivated cells showed a certain degree of efficacy against fruit decays. The ability of C. lusitaniae 146 to inhibit spore germination and its high efficiency against both sensitive and resistant fungi to fungicides were also demonstrated. Lastly, it was found that C. lusitaniae 146 could survive in natural waxes for 7 d at 25 °C and 40 d at 8 °C. Thus, our studies confirmed the potential industrial use of C. lusitaniae 146 as an effective biocontrol agent against postharvest infections of lemons caused by P. digitatum.
Yeechu Che - One of the best experts on this subject based on the ideXlab platform.
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method dependent epidemiological cutoff values for detection of triazole resistance in candida and aspergillus species for the sensititre yeastone colorimetric broth and etest agar diffusion methods
Antimicrobial Agents and Chemotherapy, 2018Co-Authors: Ana Espinelingroff, J. Turnidge, Erja Chryssanthou, Ana Alastrueyizquierdo, Francoise Otterel, E Canto, Carmen Barroso Castro, Yeechu Che, Eric DannaouiAbstract:: Although the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrata species complex, 157 C.guilliermondii (Meyerozyma guilliermondii), 676 C. krusei (Pichia kudriavzevii), 298 C.lusitaniae (Clavispora lusitaniae), 911 C.parapsilosissensu stricto, 3,691 C.parapsilosis species complex, 36 C.metapsilosis, 110 C.orthopsilosis, 1,854 C.tropicalis, 244 Saccharomyces cerevisiae, 1,409 Aspergillus fumigatus, 389 A.flavus, 130 A.nidulans, 233 A.niger, and 302 A.terreus complex isolates. SYO/Etest MICs for 282 confirmed non-wild-type (non-WT) isolates were included: ERG11 (C. albicans), ERG11 and MRR1 (C. parapsilosis), cyp51A (A. fumigatus), and CDR2 and CDR1 overexpression (C. albicans and C. glabrata, respectively). Interlaboratory modal agreement was superior by SYO for yeast species and by the Etest for Aspergillus spp. Distributions fulfilling CLSI criteria for epidemiological cutoff value (ECV) definition were pooled, and we proposed SYO ECVs for S. cerevisiae and 9 yeast and 3 Aspergillus species and Etest ECVs for 5 yeast and 4 Aspergillus species. The posaconazole SYO ECV of 0.06 µg/ml for C. albicans and the Etest itraconazole ECV of 2 µg/ml for A. fumigatus were the best predictors of non-WT isolates. These findings support the need for method-dependent ECVs, as, overall, the SYO appears to perform better for susceptibility testing of yeast species and the Etest appears to perform better for susceptibility testing of Aspergillus spp. Further evaluations should be conducted with more Candida mutants.