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Lei Xie - One of the best experts on this subject based on the ideXlab platform.
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an updated phylogenetic and biogeographic analysis based on genome skimming data reveals convergent evolution of shrubby habit in Clematis in the pliocene and pleistocene
Molecular Phylogenetics and Evolution, 2021Co-Authors: Rudan Lyu, Linying Pei, Lei Xie, Min Yao, Shuangxi Yan, Lele Lin, Yike Luo, Jiamin Xiao, Jun Wen, Jin ChengAbstract:Abstract Convergent evolution, often viewed as the inevitable outcome of natural selection, has received special attention since the time of Darwin. Clematis is well known for its climbing habit, but it has some shrubby species, known as sect. Fruticella s.l. The shrubby Clematis species are distributed in the dry habitats of Central Asia and adjacent areas showing possible convergent evolution. In this study, we assembled the complete plastome and nuclear ribosomal DNA (nrDNA) sequences of 56 Clematis species, representing most sections and covering most of the shrubby species, to reconstruct their evolutionary histories. Using both maximum likelihood and Bayesian methods, the plastome and nrDNA datasets generated similar, but not identical, phylogenetic relationships, which are better resolved than in previous studies. Then, molecular dating, historical range reconstruction, and character optimization analyses were conducted based on this updated phylogenetic framework. All the morphological characters widely used for taxonomy were shown to have evolved multiple times. Molecular dating inferred that Clematis diverged from its sister in the mid Miocene, and all six major clades of Clematis originated during the late Miocene, with a species radiation during the Pliocene to Pleistocene. The results clearly showed that the shrubby habit evolved independently in four lineages of Clematis in Asia. We also revealed that the shrubby lineages have emerged since the very beginning of Pliocene. Asian monsoon variation in the Pliocene and glacial period fluctuation in the Pleistocene may be the driving forces for the origin and diversification of the shrubby Clematis in Central Asia and adjacent dry areas.
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The complete chloroplast genome sequences of three Clematis species (Ranunculaceae)
Mitochondrial DNA Part B, 2019Co-Authors: Qiu-hong Xiang, Huijie Liu, Rudan Lyu, Min Yao, Wenbin Guan, Lei XieAbstract:Clematis is an important horticultural and medicinal genus in Ranunculaceae. Here, we report three complete chloroplast genome sequences of C. acerifolia, C. smilacifolia, and C. uncinata. The chlo...
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Clematis mae (Ranunculaceae), a new species of C. sect. Meclatis from Xinjiang, China
Pensoft Publishers, 2019Co-Authors: Rudan Lyu, Lei Xie, Min Yao, Zong-zong YangAbstract:Clematis mae Z.Z.Yang & L.Xie, a new species of Ranunculaceae from Xinjiang, China, is described and illustrated. The new species is morphologically similar to C. orientalis and C. glauca but can be distinguished for being a less hairy plant (hairy in C. orientalis), often 2-ternate leaves (1–2-pinnate for C. orientalis and C. glauca), lanceolate to linear-lanceolate leaflets (elliptic or ovate in C. glauca), larger flowers (smaller flower in C. orientalis) and narrowly lanceolate sepals with acute to slightly attenuate apex (narrowly oblong sepals in C. orientalis and ovate to broadly lanceolate sepals in C. glauca). The new species is endemic to the southern slope of North Tianshan Mountain in Central Xinjiang. The conservation status of the species is also discussed
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Table_1_Comparative Analysis of Complete Chloroplast Genomes of Anemoclema, Anemone, Pulsatilla, and Hepatica Revealing Structural Variations Among Genera in Tribe Anemoneae (Ranunculaceae).DOCX
2018Co-Authors: Huijie Liu, Chuanhua Ding, Jin Cheng, Rudan Lyu, Linying Pei, Lei XieAbstract:Structural rearrangements of Anemone species' chloroplast genome has been reported based on genetic mapping of restriction sites but has never been confirmed by genomic studies. We used a next-generation sequencing method to characterize the complete chloroplast genomes of five species in the tribe Anemoneae. Plastid genomes were assembled using de novo assembling methods combined with conventional Sanger sequencing to fill the gaps. The gene order of the chloroplast genomes of tribe Anemoneae was compared with that of other Ranunculaceae species. Multiple inversions and transpositions were detected in tribe Anemoneae. Anemoclema, Anemone, Hepatica, and Pulsatilla shared the same gene order, which contained three inversions in the large single copy region (LSC) compared to other Ranunculaceae genera. ArchiClematis, Clematis, and Naravelia shared the same gene order containing two inversions and one transposition in LSC. A roughly 4.4 kb expansion region in inverted repeat (IR) regions was detected in tribe Anemoneae, suggesting that this expansion event may be a synapomorphy for this group. Plastome phylogenomic analyses using parsimony and a Bayesian method with implementation of partitioned models generated a well resolved phylogeny of Ranunculaceae. These results suggest that evaluation of chloroplast genomes may result in improved resolution of family phylogenies. Samples of Anemone, Hepatica, and Pulsatilla were tested to form paraphyletic grades within tribe Anemoneae. Anemoclema was a sister clade to Clematis. Structual variation of the plastid genome within tribe Anemoneae provided strong phylogenetic information for Ranunculaceae.
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taxonomic status of Clematis acerifolia var elobata based on molecular evidence
Phytotaxa, 2016Co-Authors: Shuangxi Yan, Huijie Liu, Linying Pei, Lele Lin, Shuai Liao, Lei XieAbstract:The morphologically based taxonomic status of Clematis acerifolia var. elobata has been controversial. This study used two nuclear (ITS and ETS) and six plastid ( rps16 , rpl16 , accD , trnS-trnG , atpB-rbcL , and trnV-atpE ) molecular markers, and a DNA barcoding analysis to address the taxonomic status of C. acerifolia var. elobata and the relationship among other Clematis species. Our results showed that the discrimination power of ITS, ETS, and atpB-rbcL was better than that of the other tested DNA regions. When all tested sequences combined, most of the sampled taxa were resolved. Though the two taxa are closely related, they have differentiated clearly and formed two clades respectively. The mean divergence of the two taxa was 0.78%, which was higher than closely related Clematis species such as C. heracleifoli a and C. pinnata (0.50 %). Considering molecular divergence, morphological differences, and distribution area, we raised C. acerifolia var. elobata to species level.
Jin Cheng - One of the best experts on this subject based on the ideXlab platform.
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an updated phylogenetic and biogeographic analysis based on genome skimming data reveals convergent evolution of shrubby habit in Clematis in the pliocene and pleistocene
Molecular Phylogenetics and Evolution, 2021Co-Authors: Rudan Lyu, Linying Pei, Lei Xie, Min Yao, Shuangxi Yan, Lele Lin, Yike Luo, Jiamin Xiao, Jun Wen, Jin ChengAbstract:Abstract Convergent evolution, often viewed as the inevitable outcome of natural selection, has received special attention since the time of Darwin. Clematis is well known for its climbing habit, but it has some shrubby species, known as sect. Fruticella s.l. The shrubby Clematis species are distributed in the dry habitats of Central Asia and adjacent areas showing possible convergent evolution. In this study, we assembled the complete plastome and nuclear ribosomal DNA (nrDNA) sequences of 56 Clematis species, representing most sections and covering most of the shrubby species, to reconstruct their evolutionary histories. Using both maximum likelihood and Bayesian methods, the plastome and nrDNA datasets generated similar, but not identical, phylogenetic relationships, which are better resolved than in previous studies. Then, molecular dating, historical range reconstruction, and character optimization analyses were conducted based on this updated phylogenetic framework. All the morphological characters widely used for taxonomy were shown to have evolved multiple times. Molecular dating inferred that Clematis diverged from its sister in the mid Miocene, and all six major clades of Clematis originated during the late Miocene, with a species radiation during the Pliocene to Pleistocene. The results clearly showed that the shrubby habit evolved independently in four lineages of Clematis in Asia. We also revealed that the shrubby lineages have emerged since the very beginning of Pliocene. Asian monsoon variation in the Pliocene and glacial period fluctuation in the Pleistocene may be the driving forces for the origin and diversification of the shrubby Clematis in Central Asia and adjacent dry areas.
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comparative analysis of complete chloroplast genomes of anemoclema anemone pulsatilla and hepatica revealing structural variations among genera in tribe anemoneae ranunculaceae
Frontiers in Plant Science, 2018Co-Authors: Jian He, Chuanhua Ding, Jin ChengAbstract:Structural rearrangements of Anemone species’ chloroplast genome has been reported based on genetic mapping of restriction sites but has never been confirmed by genomic studies. We used a next-generation sequencing method to characterize the complete chloroplast genomes of five species in the tribe Anemoneae. Plastid genomes were assembled using de novo assembling methods combined with conventional Sanger sequencing to fill the gaps. The gene order of the chloroplast genomes of tribe Anemoneae was compared with that of other Ranunculaceae species. Multiple inversions and transpositions were detected in tribe Anemoneae. Anemoclema, Anemone, Hepatica and Pulsatilla shared the same gene order, which contained three inversions in the large single copy region (LSC) compared to other Ranunculaceae genera. ArchiClematis, Clematis, and Naravelia shared the same gene order containing two inversions and one transposition in LSC. A roughly 4.4 kb expansion region in inverted repeat (IR) regions was detected in tribe Anemoneae, suggesting that this expansion event may be a synapomorphy for this group. Plastome phylogenomic analyses using parsimony and a Bayesian method with implementation of partitioned models generated a well resolved phylogeny of Ranunculaceae. These results suggest that evaluation of chloroplast genomes may result in improved resolution of family phylogenies. Samples of Anemone, Hepatica, and Pulsatilla were tested to form paraphyletic grades within tribe Anemoneae. Anemoclema was a sister clade to Clematis. Structual variation of the plastid genome within tribe Anemoneae provided strong phylogenetic information for Ranunculaceae.
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Table_1_Comparative Analysis of Complete Chloroplast Genomes of Anemoclema, Anemone, Pulsatilla, and Hepatica Revealing Structural Variations Among Genera in Tribe Anemoneae (Ranunculaceae).DOCX
2018Co-Authors: Huijie Liu, Chuanhua Ding, Jin Cheng, Rudan Lyu, Linying Pei, Lei XieAbstract:Structural rearrangements of Anemone species' chloroplast genome has been reported based on genetic mapping of restriction sites but has never been confirmed by genomic studies. We used a next-generation sequencing method to characterize the complete chloroplast genomes of five species in the tribe Anemoneae. Plastid genomes were assembled using de novo assembling methods combined with conventional Sanger sequencing to fill the gaps. The gene order of the chloroplast genomes of tribe Anemoneae was compared with that of other Ranunculaceae species. Multiple inversions and transpositions were detected in tribe Anemoneae. Anemoclema, Anemone, Hepatica, and Pulsatilla shared the same gene order, which contained three inversions in the large single copy region (LSC) compared to other Ranunculaceae genera. ArchiClematis, Clematis, and Naravelia shared the same gene order containing two inversions and one transposition in LSC. A roughly 4.4 kb expansion region in inverted repeat (IR) regions was detected in tribe Anemoneae, suggesting that this expansion event may be a synapomorphy for this group. Plastome phylogenomic analyses using parsimony and a Bayesian method with implementation of partitioned models generated a well resolved phylogeny of Ranunculaceae. These results suggest that evaluation of chloroplast genomes may result in improved resolution of family phylogenies. Samples of Anemone, Hepatica, and Pulsatilla were tested to form paraphyletic grades within tribe Anemoneae. Anemoclema was a sister clade to Clematis. Structual variation of the plastid genome within tribe Anemoneae provided strong phylogenetic information for Ranunculaceae.
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reconsidering the phyllotaxy significance of seedlings in Clematis
Phytotaxa, 2016Co-Authors: Jin Cheng, Huijie Liu, Shuangxi Yan, Lele Lin, Shuai Liao, Lei XieAbstract:The taxonomic significance of Clematis seedling morphology has been emphasized for decades. However, seedlings of almost 75% of Clematis spp. have not been observed directly. This study germinated achenes and described the seedlings of 14 Clematis taxa, 10 of which have not been reported. The results show that five species and one variety, such as C. acerifolia , C. acerifolia var. elobata , C. urophylla , C. repens , C. brevicaudata , and C. peterae , had typical type I seedlings and that C. integrifolia and C. hexapetala had type II seedlings. Six species showed atypical seedling morphology as defined in previous studies. Our results are the first to describe that species of sect. Naravelia and sect. Fruticella have seedlings with opposite leaves. However, they were different from type II seedlings. Suppressed or elongated hypocotyls are most likely determined by the habitat temperature of the Clematis . Our results suggest that it may be inappropriate to classify Clematis seedlings into two types, and that this classification trait should be used very carefully.
Zhixin Liao - One of the best experts on this subject based on the ideXlab platform.
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first separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides by high speed counter current chromatography
Journal of Separation Science, 2016Co-Authors: Chengcheng Gong, Tao Chen, Huifeng Chen, Shanshan Zhang, Xinzhu Wang, Weiqing Wang, Jing Sun, Zhixin LiaoAbstract:In this paper, we report an efficient method by high-speed counter-current chromatography for the first separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides. Firstly, the ethyl acetate extract was treated by silica gel column chromatography to enrich the target compounds. And then the fraction with target compounds were purified by high-speed counter-counter chromatography using a two-phase solvent system consisting of chloroform/acetonitrile/water (10:6:4, v/v). The results showed high-speed counter-current chromatography could be a powerful technology for separation of compounds with similar structures and polarities. Besides, it was found acetonitrile could be a good methanol substitutes when a chloroform/methanol/water system could not provide a good separation factor. This study provides a reference for the separation compounds from Clematis akebioides. This article is protected by copyright. All rights reserved
Chengcheng Gong - One of the best experts on this subject based on the ideXlab platform.
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first separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides by high speed counter current chromatography
Journal of Separation Science, 2016Co-Authors: Chengcheng Gong, Tao Chen, Huifeng Chen, Shanshan Zhang, Xinzhu Wang, Weiqing Wang, Jing Sun, Zhixin LiaoAbstract:In this paper, we report an efficient method by high-speed counter-current chromatography for the first separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides. Firstly, the ethyl acetate extract was treated by silica gel column chromatography to enrich the target compounds. And then the fraction with target compounds were purified by high-speed counter-counter chromatography using a two-phase solvent system consisting of chloroform/acetonitrile/water (10:6:4, v/v). The results showed high-speed counter-current chromatography could be a powerful technology for separation of compounds with similar structures and polarities. Besides, it was found acetonitrile could be a good methanol substitutes when a chloroform/methanol/water system could not provide a good separation factor. This study provides a reference for the separation compounds from Clematis akebioides. This article is protected by copyright. All rights reserved
Jingkui Tian - One of the best experts on this subject based on the ideXlab platform.
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the complete chloroplast genome sequence of Clematis terniflora dc ranunculaceae
Mitochondrial DNA, 2016Co-Authors: Bingxian Yang, Qinyi Chen, Wei Zhu, Jingkui TianAbstract:Clematis terniflora DC. is an important medicinal plant used in the treatment of inflammatory symptoms related to respiratory and urinary systems. In this study, we found that the complete cp genome of C. terniflora DC. is 159,528 bp. The phylogenetic analysis of 32 taxa showed a strong sister relationship with Ranunculus macranthus, which also strongly supports the position of Ranunculales. The complete cp genome sequence of Clematis terniflora DC. reported here has the potential to advance population and phylogenetic studies of this medicinal plant.
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in vivo study of four preparative extracts of Clematis terniflora dc for antinociceptive activity and anti inflammatory activity in rat model of carrageenan induced chronic non bacterial prostatitis
Journal of Ethnopharmacology, 2011Co-Authors: Runze Chen, Lei Cui, Y J Guo, Y M Rong, Mingyao Sun, Lixia Zhang, Jingkui TianAbstract:Abstract Aim of the study The present study was conducted to evaluate the anti-inflammatory and antinociceptive activities of Clematis terniflora DC. extracts and fractions and to further support its traditional use as Chinese folk medicine in treatment of urinary infections, especially the disease of prostatitis. Materials and methods The antinociceptive activity of its water extract (WE), 70% ethanol extract (EE), water eluted part of EE from AB-8 macroporous resin (WEPMR) and 70% ethanol eluted part of EE from AB-8 macroporous resin (EEPMR) was conducted using mice writhing test with different doses. Then the anti-inflammatory activity of the four parts was evaluated on rat models of carrageenan-induced chronic non-bacterial prostatitis (CNP). Preliminary study was taken to determine the phytochemical compositions of the four preparative extracts. Results Significant writhing inhibitory effect was found with EE at small (7.5 g/kg body wt.), moderate (15 g/kg body wt.) and large (30 g/kg body wt.) doses (doses here are presented as crude herbs) as well as EEPMR at moderate and large doses by oral administration (OA) (p ≤ 0.01). Data from prostatic index, lecithin microsome density and white blood cell level showed that moderate dose of EE and EEPMR both had significant (p ≤ 0.05 or p ≤ 0.01) inhibition effect on carrageenan-induced inflammation in rat prostate. The HPLC analytical results showed that flavonoids were the main active compounds in WE, EE and EEPMR. And most flavonoids were accumulated into the part of EEPMR by AB-8 macroporous resin leaving only few compounds in WEPMR. No acute toxicity was identified in oral administration of the four parts at a dose of 100 g/kg body wt. Conclusions The results described here suggest that extracts of the aerial part of Clematis terniflora DC. might be of therapeutic interest in the treatment of prostatitis.