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Jun Hirabayashi - One of the best experts on this subject based on the ideXlab platform.
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lect hepa a triplex lectin antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside Clinical Chemistry Analyzer and an automated pretreatment machine
Clinica Chimica Acta, 2011Co-Authors: Atsushi Kuno, Yuzuru Ikehara, Yasuhito Tanaka, Kozue Saito, Kiyoaki Ito, Chikayuki Tsuruno, Shinya Nagai, Youichi Takahama, Masashi Mizokami, Jun HirabayashiAbstract:Abstract Background A quantitative analysis of glyco-alteration in serum glycoproteins provides glyco-parameters for estimating the progression of liver fibrosis. In the analysis of glycans, a manual pretreatment process for Clinical specimens leads to a complicated manipulation and loss-of-Clinical implementation of the assay. Method We evaluated an automated triplex lectin–antibody sandwich immunoassay assisted by an automated protein purification system (ED-01) and a bedside Clinical Chemistry Analyzer (HISCL) for the acquisition of two glyco-parameters (AOL/DSA and MAL/DSA) derived from a fibrosis-related glyco-alteration of serum alpha1-acid glycoprotein (AGP). Results We adjusted the auto-machines with their accuracy set to CV 0.90). The formula for monitoring fibrosis (LecT-Hepa) was given by a combination of the glyco-parameters. This correlated with the fibrosis stage from biopsy (R = 0.68) and diagnosed severe fibrosis and cirrhosis. It was superior to that of FIB-4 index. Conclusions We automated a multilectin-assisted immunoassay with an order of magnitude reduction of operation time without any loss-of-accuracy. LecT-Hepa is a reliable method to assess fibrosis-dynamics from moderate fibrosis to cirrhosis.
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LecT-Hepa: A triplex lectin–antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside Clinical Chemistry Analyzer and an automated pretreatment machine
Clinica chimica acta; international journal of clinical chemistry, 2011Co-Authors: Atsushi Kuno, Yuzuru Ikehara, Yasuhito Tanaka, Kozue Saito, Kiyoaki Ito, Chikayuki Tsuruno, Shinya Nagai, Youichi Takahama, Masashi Mizokami, Jun HirabayashiAbstract:Abstract Background A quantitative analysis of glyco-alteration in serum glycoproteins provides glyco-parameters for estimating the progression of liver fibrosis. In the analysis of glycans, a manual pretreatment process for Clinical specimens leads to a complicated manipulation and loss-of-Clinical implementation of the assay. Method We evaluated an automated triplex lectin–antibody sandwich immunoassay assisted by an automated protein purification system (ED-01) and a bedside Clinical Chemistry Analyzer (HISCL) for the acquisition of two glyco-parameters (AOL/DSA and MAL/DSA) derived from a fibrosis-related glyco-alteration of serum alpha1-acid glycoprotein (AGP). Results We adjusted the auto-machines with their accuracy set to CV 0.90). The formula for monitoring fibrosis (LecT-Hepa) was given by a combination of the glyco-parameters. This correlated with the fibrosis stage from biopsy (R = 0.68) and diagnosed severe fibrosis and cirrhosis. It was superior to that of FIB-4 index. Conclusions We automated a multilectin-assisted immunoassay with an order of magnitude reduction of operation time without any loss-of-accuracy. LecT-Hepa is a reliable method to assess fibrosis-dynamics from moderate fibrosis to cirrhosis.
Mitchell G Scott - One of the best experts on this subject based on the ideXlab platform.
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Performance of a no-pretreatment tacrolimus assay on the Dade Behring Dimension RxL Clinical Chemistry Analyzer.
Clinica chimica acta; international journal of clinical chemistry, 2007Co-Authors: Megan A Griffey, Karl G Hock, Daniel C Kilgore, Tie Q Wei, Show-hong Duh, Robert Christenson, Mitchell G ScottAbstract:Therapeutic drug monitoring for tacrolimus is important for organ transplant patients receiving this immunosuppressant. Current available assays for tacrolimus require sample pre-treatment and operate in a batch mode. Here a no-pretreatment tacrolimus assay, performed on the Dade Behring Dimension Analyzer is compared to the Abbott IMx tacrolimus assay and to an LC/MS/MS method. Whole blood samples from 2 medical centers and different transplant types (kidney n=103, liver n=81, heart n=27, pancreas n=16, bone marrow n=9, [corrected] lung n=7), were obtained and tacrolimus quantified by each of the 3 assays. The lower limit of the linear range was 1.2 ng/ml on the Dimension assay. Total imprecision was 9.8% and within-run imprecision was 9.6% at a tacrolimus concentration of 3.4 ng/dL. Passing-Bablock regression analysis determined the following relationships: DIMN=(1.16) LC/MS - 0.43, r=0.90 and DIMN=(0.99)IMx - 0.35, r=0.87. The Dade Behring Dimension [corrected] Tacrolimus assay has adequate imprecision and correlates well with the reference method of LC/MS/MS. The assay appears suitable for Clinical use, and has the advantages of not requiring a pretreatment step and the ability to be performed in a random-access mode.
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Performance of a no-pretreatment tacrolimus assay on the Dade Behring Dimension RxL Clinical Chemistry Analyzer.
Clinica Chimica Acta, 2007Co-Authors: Megan A Griffey, Karl G Hock, Daniel C Kilgore, Tie Q Wei, Show-hong Duh, Robert H. Christenson, Mitchell G ScottAbstract:Abstract Background Therapeutic drug monitoring for tacrolimus is important for organ transplant patients receiving this immunosuppressant. Current available assays for tacrolimus require sample pre-treatment and operate in a batch mode. Here a no-pretreatment tacrolimus assay, performed on the Dade Behring Dimension Analyzer is compared to the Abbott IMx tacrolimus assay and to an LC/MS/MS method. Methods Whole blood samples from 2 medical centers and different transplant types (kidney n = 104, liver n = 84, heart n = 28, pancreas n = 16, bone marrow n = 11, lung n = 7), were obtained and tacrolimus quantified by each of the 3 assays. Results The lower limit of the linear range was 1.2 ng/ml on the Dimension assay. Total imprecision was 9.8% and within-run imprecision was 9.6% at a tacrolimus concentration of 3.4 ng/dL. Passing–Bablock regression analysis determined the following relationships: DIMN = (1.16) LC/MS — 0.43, r = 0.90 and DIMN = (0.99)IMx — 0.35, r = 0.87. Conclusions The Dade Behring Dimension II Tacrolimus assay has adequate imprecision and correlates well with the reference method of LC/MS/MS. The assay appears suitable for Clinical use, and has the advantages of not requiring a pretreatment step and the ability to be performed in a random-access mode.
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Evaluation of a no-pretreatment cyclosporin A assay on the Dade Behring dimension RxL Clinical Chemistry Analyzer.
Clinical chemistry, 2002Co-Authors: Andrea R. Terrell, Karl G Hock, Daniel C Kilgore, Tie Q Wei, Thomas M. Daly, Sharon Hernandez, Don Weibe, Leona Fields, Leslie M. Shaw, Mitchell G ScottAbstract:Background: Monitoring whole-blood concentrations of cyclosporin A (CsA) is common practice in the management of solid organ and bone marrow transplant recipients. In a multicenter study we evaluated a new, direct (no pretreatment) CsA assay on the Dade Behring Dimension RxLTM system and compared results with those from the Abbott TDx CsA immunoassay and a HPLC method. Methods: Whole-blood samples from heart (n = 111; 35 patients), liver (n = 201; 44 patients), kidney (n = 279; 65 patients), and miscellaneous organ (n = 77; 12 lung, 12 bone marrow, 5 kidney/pancreas, and 1 pancreas patient) recipients were obtained from patient populations of the participating institutions. Routine Clinical monitoring of CsA was performed using either the TDx method or HPLC. Results: The minimum detectable concentration of CsA averaged 9.4 μg/L, and the lower limit of quantification was 30 μg/L. The method was linear from 30 to 500 μg/L. Cross-reactivity with seven different CsA metabolites ranged from 0.0% to 5.7% for the Dimension RxL assay compared with 0.4–15.9% for the TDx assay. Total imprecision (CV) averaged 6.2%, and within-run imprecision averaged 4.9%. Passing–Bablok linear regression analyses of all samples from two sites yielded the following: RxL = 0.81 × TDx − 16.8; and RxL = 1.12 × HPLC − 1.7. Conclusions: The Dade Behring CsA assay for the random-access Dimension platform offers adequate performance characteristics for routine Clinical use, does not require a manual pretreatment step, and demonstrates less cross-reactivity with CsA metabolites than another commonly used immunoassay.
Viroj Wiwanitkit - One of the best experts on this subject based on the ideXlab platform.
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AccucheckInform II: About Its Accuracy and Precision
iranian journal of pathology, 2014Co-Authors: Beuy Joob, Viroj WiwanitkitAbstract:To introduce the most easy to performblood glucose investigation can help management of diabetes mellitus. The point of care testing (POCT) technology is widely used and there are many new introduced techniques (1). The AccuCheck Inform II is a new technique with use of a mutated glucose dehydrogenase that has no problem of cross-reaction to maltose (2). The previous report showed that this new tool is acceptable for Clinical use. Here, the authors perform a study on the accuracy and precision of theAccuCheck Inform II comparing to Lab Cobas6000, a standard Clinical Chemistry Analyzer (N = 2for both accuracy and precision study). Focusing on our results, all samples pass the evaluation criteria (accuracy more than 90% and imprecision less than 10%). It can be seen that the results support the previous report by Dietzen et al.(2). Nevertheless, there are still more concerns for further evaluation. First, the cost effectiveness of the new technique comparing to the classical tool has to be studied. Second, although the new technique has decreased problem of laboratory interference, there is still a report on the concerned interference from icodextrin metabolites (3).
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Laboratory References Parameters for Basic Functional Tests Among the Thais
2011Co-Authors: Viroj Wiwanitkit, Kawin TangdhanakanondAbstract:Objective : To set up new Clinical Chemistry Laboratory tests references parameters among the Thais in Bangkok Study Design: A descriptive study 712 normal subjects of both sex attending the annual routine check up program of King Chulalongkom Memorial Hospital Blood specimen from each subject was collected using the evacuated blood collection system. Then 5 important Clinical Chemistry parameter including BUN, Creatinine, SGOT, SGPT and ALP were analyzed using automated Clinical Chemistry Analyzer. Then reference ranges were calculated. Results : The reference values of each parameter was set and described in the article. Conclusion: Reference value setting is necessary and should be found in each laboratory setting Keywords : Clinical Chemistry, reference
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Reproductive Hormone Tests References Parameters
2011Co-Authors: Viroj WiwanitkitAbstract:Objective: To set up new reproductive hormone tests references parameters among the Thais in Bangkok Study Design : A descriptive study Subjects: 100 normal subjects (50 for each sex) attending the routine check up program of King Chulalongkorn Memorial Hospital Method: Blood specimen from each subject was collected using the evacuated blood collection system. Then 6 important Clinical Chemistry parameters including estradiol (E2), progesterone, FSH, LH, prolactin and testosterone were analyzed using automated Clinical Chemistry Analyzer. Then reference ranges were calculated. Results: The reference values of each parameter was set and described in the article. Conclusion : Reference value setting is necessary and should be found in each laboratory setting. Keywords: reproductive hormone test, reference
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Thyroid Function Tests References Parameters
2011Co-Authors: Viroj WiwanitkitAbstract:Objective: To set up new thyroid function tests references parameters among the Thais in Bangkok Study Design: A descriptive study Subjects: 100 normal subjects of both sexes attending the routine check up program of King Chulalongkorn Memorial Hospital Method: Blood specimen from each subject was collected using the evacuated blood collection system. Then 5 important Clinical Chemistry parameters including T3, T4, FT3, FT4 and TSH were analyzed using automated Clinical Chemistry Analyzer. Then reference ranges were calculated. Results: The reference values of each parameter were set and described in the article. Conclusion: Reference value setting is necessary and should be found in each laboratory setting. Keywords: thyroid function test, reference
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Tumor Marker References Parameters
2011Co-Authors: Viroj WiwanitkitAbstract:Objective : To set up new tumor marker references parameters among the Thais in Bangkok Study Design : A descriptive study Subjects: 100 normal subjects from both sexes attending the routine check up program of King Chulalongkorn Memorial Hospital Method: Blood specimen from each subject was collected using the evacuated blood collection system. Then 7 important Clinical Chemistry parameters including AFP, CEA, hCG, CA125, CA19-9, CA15-3, and PSA were analyzed using automated Clinical Chemistry Analyzer. Then reference ranges were calculated. Results: The reference values of each parameter were set and described in the article. Conclusion: Reference value setting is necessary and should be found in each laboratory setting. Keywords: tumor marker, reference
Atsushi Kuno - One of the best experts on this subject based on the ideXlab platform.
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lect hepa a triplex lectin antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside Clinical Chemistry Analyzer and an automated pretreatment machine
Clinica Chimica Acta, 2011Co-Authors: Atsushi Kuno, Yuzuru Ikehara, Yasuhito Tanaka, Kozue Saito, Kiyoaki Ito, Chikayuki Tsuruno, Shinya Nagai, Youichi Takahama, Masashi Mizokami, Jun HirabayashiAbstract:Abstract Background A quantitative analysis of glyco-alteration in serum glycoproteins provides glyco-parameters for estimating the progression of liver fibrosis. In the analysis of glycans, a manual pretreatment process for Clinical specimens leads to a complicated manipulation and loss-of-Clinical implementation of the assay. Method We evaluated an automated triplex lectin–antibody sandwich immunoassay assisted by an automated protein purification system (ED-01) and a bedside Clinical Chemistry Analyzer (HISCL) for the acquisition of two glyco-parameters (AOL/DSA and MAL/DSA) derived from a fibrosis-related glyco-alteration of serum alpha1-acid glycoprotein (AGP). Results We adjusted the auto-machines with their accuracy set to CV 0.90). The formula for monitoring fibrosis (LecT-Hepa) was given by a combination of the glyco-parameters. This correlated with the fibrosis stage from biopsy (R = 0.68) and diagnosed severe fibrosis and cirrhosis. It was superior to that of FIB-4 index. Conclusions We automated a multilectin-assisted immunoassay with an order of magnitude reduction of operation time without any loss-of-accuracy. LecT-Hepa is a reliable method to assess fibrosis-dynamics from moderate fibrosis to cirrhosis.
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LecT-Hepa: A triplex lectin–antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside Clinical Chemistry Analyzer and an automated pretreatment machine
Clinica chimica acta; international journal of clinical chemistry, 2011Co-Authors: Atsushi Kuno, Yuzuru Ikehara, Yasuhito Tanaka, Kozue Saito, Kiyoaki Ito, Chikayuki Tsuruno, Shinya Nagai, Youichi Takahama, Masashi Mizokami, Jun HirabayashiAbstract:Abstract Background A quantitative analysis of glyco-alteration in serum glycoproteins provides glyco-parameters for estimating the progression of liver fibrosis. In the analysis of glycans, a manual pretreatment process for Clinical specimens leads to a complicated manipulation and loss-of-Clinical implementation of the assay. Method We evaluated an automated triplex lectin–antibody sandwich immunoassay assisted by an automated protein purification system (ED-01) and a bedside Clinical Chemistry Analyzer (HISCL) for the acquisition of two glyco-parameters (AOL/DSA and MAL/DSA) derived from a fibrosis-related glyco-alteration of serum alpha1-acid glycoprotein (AGP). Results We adjusted the auto-machines with their accuracy set to CV 0.90). The formula for monitoring fibrosis (LecT-Hepa) was given by a combination of the glyco-parameters. This correlated with the fibrosis stage from biopsy (R = 0.68) and diagnosed severe fibrosis and cirrhosis. It was superior to that of FIB-4 index. Conclusions We automated a multilectin-assisted immunoassay with an order of magnitude reduction of operation time without any loss-of-accuracy. LecT-Hepa is a reliable method to assess fibrosis-dynamics from moderate fibrosis to cirrhosis.
Yuzuru Ikehara - One of the best experts on this subject based on the ideXlab platform.
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lect hepa a triplex lectin antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside Clinical Chemistry Analyzer and an automated pretreatment machine
Clinica Chimica Acta, 2011Co-Authors: Atsushi Kuno, Yuzuru Ikehara, Yasuhito Tanaka, Kozue Saito, Kiyoaki Ito, Chikayuki Tsuruno, Shinya Nagai, Youichi Takahama, Masashi Mizokami, Jun HirabayashiAbstract:Abstract Background A quantitative analysis of glyco-alteration in serum glycoproteins provides glyco-parameters for estimating the progression of liver fibrosis. In the analysis of glycans, a manual pretreatment process for Clinical specimens leads to a complicated manipulation and loss-of-Clinical implementation of the assay. Method We evaluated an automated triplex lectin–antibody sandwich immunoassay assisted by an automated protein purification system (ED-01) and a bedside Clinical Chemistry Analyzer (HISCL) for the acquisition of two glyco-parameters (AOL/DSA and MAL/DSA) derived from a fibrosis-related glyco-alteration of serum alpha1-acid glycoprotein (AGP). Results We adjusted the auto-machines with their accuracy set to CV 0.90). The formula for monitoring fibrosis (LecT-Hepa) was given by a combination of the glyco-parameters. This correlated with the fibrosis stage from biopsy (R = 0.68) and diagnosed severe fibrosis and cirrhosis. It was superior to that of FIB-4 index. Conclusions We automated a multilectin-assisted immunoassay with an order of magnitude reduction of operation time without any loss-of-accuracy. LecT-Hepa is a reliable method to assess fibrosis-dynamics from moderate fibrosis to cirrhosis.
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LecT-Hepa: A triplex lectin–antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside Clinical Chemistry Analyzer and an automated pretreatment machine
Clinica chimica acta; international journal of clinical chemistry, 2011Co-Authors: Atsushi Kuno, Yuzuru Ikehara, Yasuhito Tanaka, Kozue Saito, Kiyoaki Ito, Chikayuki Tsuruno, Shinya Nagai, Youichi Takahama, Masashi Mizokami, Jun HirabayashiAbstract:Abstract Background A quantitative analysis of glyco-alteration in serum glycoproteins provides glyco-parameters for estimating the progression of liver fibrosis. In the analysis of glycans, a manual pretreatment process for Clinical specimens leads to a complicated manipulation and loss-of-Clinical implementation of the assay. Method We evaluated an automated triplex lectin–antibody sandwich immunoassay assisted by an automated protein purification system (ED-01) and a bedside Clinical Chemistry Analyzer (HISCL) for the acquisition of two glyco-parameters (AOL/DSA and MAL/DSA) derived from a fibrosis-related glyco-alteration of serum alpha1-acid glycoprotein (AGP). Results We adjusted the auto-machines with their accuracy set to CV 0.90). The formula for monitoring fibrosis (LecT-Hepa) was given by a combination of the glyco-parameters. This correlated with the fibrosis stage from biopsy (R = 0.68) and diagnosed severe fibrosis and cirrhosis. It was superior to that of FIB-4 index. Conclusions We automated a multilectin-assisted immunoassay with an order of magnitude reduction of operation time without any loss-of-accuracy. LecT-Hepa is a reliable method to assess fibrosis-dynamics from moderate fibrosis to cirrhosis.