The Experts below are selected from a list of 72 Experts worldwide ranked by ideXlab platform

James V Guarrera - One of the best experts on this subject based on the ideXlab platform.

  • hypothermic machine preservation facilitates successful Transplantation of orphan extended criteria donor Livers
    American Journal of Transplantation, 2015
    Co-Authors: James V Guarrera, Tomoaki Kato, Benjamin Samstein, Scot D Henry, E Reznik, C Musat, T Lukose, Lloyd E Ratner, Robert S Brown, Jean C Emond
    Abstract:

    Hypothermic machine preservation (HMP) remains investigational in Clinical Liver Transplantation. It is widely used to preserve kidneys for Transplantation with improved results over static cold storage (SCS). At our center, we have used HMP in 31 adults receiving extended criteria donor (ECD) Livers declined by the originating United Network for Organ Sharing region ("orphan Livers"). These cases were compared to ECD SCS cases in a matched cohort study design. Livers were matched for donor age, recipient age, cold ischemic time, donor risk index and Model for End-Stage Liver Disease (MELD) score. HMP was performed for 3-7 h at 4-8 °C using our previously published protocol. Early allograft dysfunction rates were 19% in the HMP group versus 30% in the control group (p = 0.384). One-year patient survival was 84% in the HMP group versus 80% in the SCS group (p = NS). Post hoc analysis revealed significantly less biliary complications in the HMP group versus the SCS group (4 vs. 13, p = 0.016). Mean hospital stay was significantly shorter in the HMP group (13.64 ± 10.9 vs. 20.14 ± 11.12 days in the SCS group, p = 0.001). HMP provided safe and reliable preservation in orphan Livers transplanted at our center.

  • hypothermic machine preservation reduces molecular markers of ischemia reperfusion injury in human Liver Transplantation
    American Journal of Transplantation, 2012
    Co-Authors: S D Henry, E Nachber, Jacob E Tulipan, J Stone, C Bae, L Reznik, Tomoaki Kato, Benjamin Samstein, Jean C Emond, James V Guarrera
    Abstract:

    Hypothermic machine perfusion (HMP) is in its infancy in Clinical Liver Transplantation. Potential benefits include diminished preservation injury (PI) and improved graft function. Molecular data to date has been limited to extrapolation of animal studies. We analyzed Liver tissue and serum collected during our Phase 1 trial of Liver HMP. Grafts preserved with HMP were compared to static cold stored (SCS) transplant controls. Reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry and transmission electron microscopy (TEM) were performed on Liver biopsies. Expression of inflammatory cytokines, adhesion molecules and chemokines, oxidation markers, apoptosis and acute phase proteins and the levels of CD68 positive macrophages in tissue sections were evaluated. RT-PCR of reperfusion biopsy samples in the SCS group showed high expression of inflammatory cytokines, adhesion molecules and chemokines, oxidative markers and acute phase proteins. This upregulation was significantly attenuated in Livers that were preserved by HMP. Immunofluorescence showed larger numbers of CD68 positive macrophages in the SCS group when compared to the HMP group. TEM samples also revealed ultrastructural damage in the SCS group that was not seen in the HMP group. HMP significantly reduced proinflammatory cytokine expression, relieving the downstream activation of adhesion molecules and migration of leukocytes, including neutrophils and macrophages when compared to SCS controls.

Jean C Emond - One of the best experts on this subject based on the ideXlab platform.

  • hypothermic machine preservation facilitates successful Transplantation of orphan extended criteria donor Livers
    American Journal of Transplantation, 2015
    Co-Authors: James V Guarrera, Tomoaki Kato, Benjamin Samstein, Scot D Henry, E Reznik, C Musat, T Lukose, Lloyd E Ratner, Robert S Brown, Jean C Emond
    Abstract:

    Hypothermic machine preservation (HMP) remains investigational in Clinical Liver Transplantation. It is widely used to preserve kidneys for Transplantation with improved results over static cold storage (SCS). At our center, we have used HMP in 31 adults receiving extended criteria donor (ECD) Livers declined by the originating United Network for Organ Sharing region ("orphan Livers"). These cases were compared to ECD SCS cases in a matched cohort study design. Livers were matched for donor age, recipient age, cold ischemic time, donor risk index and Model for End-Stage Liver Disease (MELD) score. HMP was performed for 3-7 h at 4-8 °C using our previously published protocol. Early allograft dysfunction rates were 19% in the HMP group versus 30% in the control group (p = 0.384). One-year patient survival was 84% in the HMP group versus 80% in the SCS group (p = NS). Post hoc analysis revealed significantly less biliary complications in the HMP group versus the SCS group (4 vs. 13, p = 0.016). Mean hospital stay was significantly shorter in the HMP group (13.64 ± 10.9 vs. 20.14 ± 11.12 days in the SCS group, p = 0.001). HMP provided safe and reliable preservation in orphan Livers transplanted at our center.

  • hypothermic machine preservation reduces molecular markers of ischemia reperfusion injury in human Liver Transplantation
    American Journal of Transplantation, 2012
    Co-Authors: S D Henry, E Nachber, Jacob E Tulipan, J Stone, C Bae, L Reznik, Tomoaki Kato, Benjamin Samstein, Jean C Emond, James V Guarrera
    Abstract:

    Hypothermic machine perfusion (HMP) is in its infancy in Clinical Liver Transplantation. Potential benefits include diminished preservation injury (PI) and improved graft function. Molecular data to date has been limited to extrapolation of animal studies. We analyzed Liver tissue and serum collected during our Phase 1 trial of Liver HMP. Grafts preserved with HMP were compared to static cold stored (SCS) transplant controls. Reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry and transmission electron microscopy (TEM) were performed on Liver biopsies. Expression of inflammatory cytokines, adhesion molecules and chemokines, oxidation markers, apoptosis and acute phase proteins and the levels of CD68 positive macrophages in tissue sections were evaluated. RT-PCR of reperfusion biopsy samples in the SCS group showed high expression of inflammatory cytokines, adhesion molecules and chemokines, oxidative markers and acute phase proteins. This upregulation was significantly attenuated in Livers that were preserved by HMP. Immunofluorescence showed larger numbers of CD68 positive macrophages in the SCS group when compared to the HMP group. TEM samples also revealed ultrastructural damage in the SCS group that was not seen in the HMP group. HMP significantly reduced proinflammatory cytokine expression, relieving the downstream activation of adhesion molecules and migration of leukocytes, including neutrophils and macrophages when compared to SCS controls.

Hidetaka Hara - One of the best experts on this subject based on the ideXlab platform.

  • involvement of tumor necrosis factor α receptor 1 and tumor necrosis factor related apoptosis inducing ligand trail receptor 2 dr 5 but not fas in graft injury in live donor Liver Transplantation
    Transplant International, 2004
    Co-Authors: Hirotaka Tashiro, Toshiyuki Itamoto, Hideki Ohdan, K Arihiro, Yuko Tateaki, Hideki Nakahara, Makoto Ochi, Hiroshi Hino, Kazuyuki Mizunuma, Hidetaka Hara
    Abstract:

    The pathway leading to cell death in Clinical Liver Transplantation is not known. Eight Liver transplant recipients and eight donors were enrolled in this study. Postoperative serum levels of alanine transferase had significantly increased in the recipients compared with those in the donors. Mild centri-lobular necrosis was observed in only Liver tissues taken from the recipients. Tumor necrosis factor (TNF)-R1 and death receptor 5 expression levels had increased in Liver tissues taken from the recipients. There were no changes in the levels of Fas/Fas ligand expression in Liver tissues from either the donors or recipients. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) expression was down-regulated in donor Liver after hepatectomy and Liver allograft after implantation. The results suggest that, although ischemic Liver injury was not serious, due to the short ischemia time, TNF and TRAIL signals are associated with Liver ischemic injury in live-donor Liver Transplantation but Fas signal is not

Tomoaki Kato - One of the best experts on this subject based on the ideXlab platform.

  • hypothermic machine preservation facilitates successful Transplantation of orphan extended criteria donor Livers
    American Journal of Transplantation, 2015
    Co-Authors: James V Guarrera, Tomoaki Kato, Benjamin Samstein, Scot D Henry, E Reznik, C Musat, T Lukose, Lloyd E Ratner, Robert S Brown, Jean C Emond
    Abstract:

    Hypothermic machine preservation (HMP) remains investigational in Clinical Liver Transplantation. It is widely used to preserve kidneys for Transplantation with improved results over static cold storage (SCS). At our center, we have used HMP in 31 adults receiving extended criteria donor (ECD) Livers declined by the originating United Network for Organ Sharing region ("orphan Livers"). These cases were compared to ECD SCS cases in a matched cohort study design. Livers were matched for donor age, recipient age, cold ischemic time, donor risk index and Model for End-Stage Liver Disease (MELD) score. HMP was performed for 3-7 h at 4-8 °C using our previously published protocol. Early allograft dysfunction rates were 19% in the HMP group versus 30% in the control group (p = 0.384). One-year patient survival was 84% in the HMP group versus 80% in the SCS group (p = NS). Post hoc analysis revealed significantly less biliary complications in the HMP group versus the SCS group (4 vs. 13, p = 0.016). Mean hospital stay was significantly shorter in the HMP group (13.64 ± 10.9 vs. 20.14 ± 11.12 days in the SCS group, p = 0.001). HMP provided safe and reliable preservation in orphan Livers transplanted at our center.

  • hypothermic machine preservation reduces molecular markers of ischemia reperfusion injury in human Liver Transplantation
    American Journal of Transplantation, 2012
    Co-Authors: S D Henry, E Nachber, Jacob E Tulipan, J Stone, C Bae, L Reznik, Tomoaki Kato, Benjamin Samstein, Jean C Emond, James V Guarrera
    Abstract:

    Hypothermic machine perfusion (HMP) is in its infancy in Clinical Liver Transplantation. Potential benefits include diminished preservation injury (PI) and improved graft function. Molecular data to date has been limited to extrapolation of animal studies. We analyzed Liver tissue and serum collected during our Phase 1 trial of Liver HMP. Grafts preserved with HMP were compared to static cold stored (SCS) transplant controls. Reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry and transmission electron microscopy (TEM) were performed on Liver biopsies. Expression of inflammatory cytokines, adhesion molecules and chemokines, oxidation markers, apoptosis and acute phase proteins and the levels of CD68 positive macrophages in tissue sections were evaluated. RT-PCR of reperfusion biopsy samples in the SCS group showed high expression of inflammatory cytokines, adhesion molecules and chemokines, oxidative markers and acute phase proteins. This upregulation was significantly attenuated in Livers that were preserved by HMP. Immunofluorescence showed larger numbers of CD68 positive macrophages in the SCS group when compared to the HMP group. TEM samples also revealed ultrastructural damage in the SCS group that was not seen in the HMP group. HMP significantly reduced proinflammatory cytokine expression, relieving the downstream activation of adhesion molecules and migration of leukocytes, including neutrophils and macrophages when compared to SCS controls.

Benjamin Samstein - One of the best experts on this subject based on the ideXlab platform.

  • hypothermic machine preservation facilitates successful Transplantation of orphan extended criteria donor Livers
    American Journal of Transplantation, 2015
    Co-Authors: James V Guarrera, Tomoaki Kato, Benjamin Samstein, Scot D Henry, E Reznik, C Musat, T Lukose, Lloyd E Ratner, Robert S Brown, Jean C Emond
    Abstract:

    Hypothermic machine preservation (HMP) remains investigational in Clinical Liver Transplantation. It is widely used to preserve kidneys for Transplantation with improved results over static cold storage (SCS). At our center, we have used HMP in 31 adults receiving extended criteria donor (ECD) Livers declined by the originating United Network for Organ Sharing region ("orphan Livers"). These cases were compared to ECD SCS cases in a matched cohort study design. Livers were matched for donor age, recipient age, cold ischemic time, donor risk index and Model for End-Stage Liver Disease (MELD) score. HMP was performed for 3-7 h at 4-8 °C using our previously published protocol. Early allograft dysfunction rates were 19% in the HMP group versus 30% in the control group (p = 0.384). One-year patient survival was 84% in the HMP group versus 80% in the SCS group (p = NS). Post hoc analysis revealed significantly less biliary complications in the HMP group versus the SCS group (4 vs. 13, p = 0.016). Mean hospital stay was significantly shorter in the HMP group (13.64 ± 10.9 vs. 20.14 ± 11.12 days in the SCS group, p = 0.001). HMP provided safe and reliable preservation in orphan Livers transplanted at our center.

  • hypothermic machine preservation reduces molecular markers of ischemia reperfusion injury in human Liver Transplantation
    American Journal of Transplantation, 2012
    Co-Authors: S D Henry, E Nachber, Jacob E Tulipan, J Stone, C Bae, L Reznik, Tomoaki Kato, Benjamin Samstein, Jean C Emond, James V Guarrera
    Abstract:

    Hypothermic machine perfusion (HMP) is in its infancy in Clinical Liver Transplantation. Potential benefits include diminished preservation injury (PI) and improved graft function. Molecular data to date has been limited to extrapolation of animal studies. We analyzed Liver tissue and serum collected during our Phase 1 trial of Liver HMP. Grafts preserved with HMP were compared to static cold stored (SCS) transplant controls. Reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry and transmission electron microscopy (TEM) were performed on Liver biopsies. Expression of inflammatory cytokines, adhesion molecules and chemokines, oxidation markers, apoptosis and acute phase proteins and the levels of CD68 positive macrophages in tissue sections were evaluated. RT-PCR of reperfusion biopsy samples in the SCS group showed high expression of inflammatory cytokines, adhesion molecules and chemokines, oxidative markers and acute phase proteins. This upregulation was significantly attenuated in Livers that were preserved by HMP. Immunofluorescence showed larger numbers of CD68 positive macrophages in the SCS group when compared to the HMP group. TEM samples also revealed ultrastructural damage in the SCS group that was not seen in the HMP group. HMP significantly reduced proinflammatory cytokine expression, relieving the downstream activation of adhesion molecules and migration of leukocytes, including neutrophils and macrophages when compared to SCS controls.