The Experts below are selected from a list of 15612 Experts worldwide ranked by ideXlab platform
R Ceulemans - One of the best experts on this subject based on the ideXlab platform.
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Clonal Variation in heavy metal accumulation and biomass production in a poplar coppice culture ii vertical distribution and phytoextraction potential
Environmental Pollution, 2005Co-Authors: I Laureysens, L De Temmerman, T Hastir, M Van Gysel, R CeulemansAbstract:Short rotation coppice cultures (SRC) are intensively managed, high-density plantations of multi-shoot trees. In April 1996, an SRC field trial with 17 different poplar clones was established in Boom (Belgium) on a former waste disposal site. In December 1996 and January 2001, all shoots were cut back to a height of 5 cm to create a coppice culture. For six clones, wood and bark were sampled at the bottom, middle and top of a shoot in August and November 2002. No significant height effect of metal concentration was found, but for wood, metal concentrations generally increased toward the top of the shoot in August, and decreased toward the top of the shoot in November. Phytoextraction potential of a clone was primarily determined by metal concentration and by biomass production. Shoot size and number of shoots per stool were less important, as a high biomass production could be achieved by producing a few large shoots or many smaller shoots. Clone Fritzi Pauley accumulated 1.4 kg ha(-1) of Al over two years; Wolterson and Balsam Spire showed a relatively high accumulation of Cd and Zn, i.e. averaging, respectively 47 and 57 g ha(-1) for Cd and 2.4 and 2.0 kg ha(-1) for Zn over two years.
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Clonal Variation in heavy metal accumulation and biomass production in a poplar coppice culture i seasonal Variation in leaf wood and bark concentrations
Environmental Pollution, 2004Co-Authors: I Laureysens, L De Temmerman, Ronny Blust, Cmhm Lemmens, R CeulemansAbstract:The use of plants to decontaminate soils polluted by heavy metals has received considerable attention in recent years as a low-cost technique. Poplars (Populus spp.) can accumulate relatively high levels of certain metals, and have the added advantage of producing biomass that can be used for energy production. A short rotation coppice culture with 13 poplar clones was established on a former waste disposal site, which was moderately polluted with heavy metals. Total content of metals in leaves, wood and bark were determined in August and October/November. Significant Clonal differences in accumulation were found for most metals, although clones with the highest concentration of all metals were not found. Cadmium, zinc and aluminium were most efficiently taken up. The lowest concentration was found in wood; the highest concentrations were generally found in senescing leaves, making removal and treatment of fallen leaves necessary.
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growth and production of a short rotation coppice culture of poplar i Clonal differences in leaf characteristics in relation to biomass production
Biomass & Bioenergy, 2004Co-Authors: A Pellis, I Laureysens, R CeulemansAbstract:Abstract Seventeen different poplar (Populus) clones were studied during the first growing season of the second rotation of a high-density coppice culture. In August 2001, total leaf area (tLA), number of leaves and specific leaf area (SLA) were determined for 15 shoots per clone. Above-ground woody biomass production and leaf area index (LAI) were estimated by using allometric relationships and an up-scaling approach. Significant Clonal Variation was observed in LAI, biomass production, tLA and number of leaves per basal shoot area. Biomass production ranged from 3 to 8 Mg ha −1 y −1 and LAI ranged from 2 to 6 m 2 m −2 . The LAI, tLA per shoot, and SLA were the most important determinants of above-ground woody biomass production. The production of many small leaves was shown to give a similar biomass production as the production of few but large leaves.
I Laureysens - One of the best experts on this subject based on the ideXlab platform.
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Clonal Variation in heavy metal accumulation and biomass production in a poplar coppice culture ii vertical distribution and phytoextraction potential
Environmental Pollution, 2005Co-Authors: I Laureysens, L De Temmerman, T Hastir, M Van Gysel, R CeulemansAbstract:Short rotation coppice cultures (SRC) are intensively managed, high-density plantations of multi-shoot trees. In April 1996, an SRC field trial with 17 different poplar clones was established in Boom (Belgium) on a former waste disposal site. In December 1996 and January 2001, all shoots were cut back to a height of 5 cm to create a coppice culture. For six clones, wood and bark were sampled at the bottom, middle and top of a shoot in August and November 2002. No significant height effect of metal concentration was found, but for wood, metal concentrations generally increased toward the top of the shoot in August, and decreased toward the top of the shoot in November. Phytoextraction potential of a clone was primarily determined by metal concentration and by biomass production. Shoot size and number of shoots per stool were less important, as a high biomass production could be achieved by producing a few large shoots or many smaller shoots. Clone Fritzi Pauley accumulated 1.4 kg ha(-1) of Al over two years; Wolterson and Balsam Spire showed a relatively high accumulation of Cd and Zn, i.e. averaging, respectively 47 and 57 g ha(-1) for Cd and 2.4 and 2.0 kg ha(-1) for Zn over two years.
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Clonal Variation in heavy metal accumulation and biomass production in a poplar coppice culture i seasonal Variation in leaf wood and bark concentrations
Environmental Pollution, 2004Co-Authors: I Laureysens, L De Temmerman, Ronny Blust, Cmhm Lemmens, R CeulemansAbstract:The use of plants to decontaminate soils polluted by heavy metals has received considerable attention in recent years as a low-cost technique. Poplars (Populus spp.) can accumulate relatively high levels of certain metals, and have the added advantage of producing biomass that can be used for energy production. A short rotation coppice culture with 13 poplar clones was established on a former waste disposal site, which was moderately polluted with heavy metals. Total content of metals in leaves, wood and bark were determined in August and October/November. Significant Clonal differences in accumulation were found for most metals, although clones with the highest concentration of all metals were not found. Cadmium, zinc and aluminium were most efficiently taken up. The lowest concentration was found in wood; the highest concentrations were generally found in senescing leaves, making removal and treatment of fallen leaves necessary.
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growth and production of a short rotation coppice culture of poplar i Clonal differences in leaf characteristics in relation to biomass production
Biomass & Bioenergy, 2004Co-Authors: A Pellis, I Laureysens, R CeulemansAbstract:Abstract Seventeen different poplar (Populus) clones were studied during the first growing season of the second rotation of a high-density coppice culture. In August 2001, total leaf area (tLA), number of leaves and specific leaf area (SLA) were determined for 15 shoots per clone. Above-ground woody biomass production and leaf area index (LAI) were estimated by using allometric relationships and an up-scaling approach. Significant Clonal Variation was observed in LAI, biomass production, tLA and number of leaves per basal shoot area. Biomass production ranged from 3 to 8 Mg ha −1 y −1 and LAI ranged from 2 to 6 m 2 m −2 . The LAI, tLA per shoot, and SLA were the most important determinants of above-ground woody biomass production. The production of many small leaves was shown to give a similar biomass production as the production of few but large leaves.
Helene Faustrup Kildegaard - One of the best experts on this subject based on the ideXlab platform.
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minimizing Clonal Variation during mammalian cell line engineering for improved systems biology data generation
ACS Synthetic Biology, 2018Co-Authors: Lise Marie Grav, Lars K. Nielsen, Daria Sergeeva, Jae Seong Lee, Igor Marin De Mas, Nathan E Lewis, Mikael Rordam Andersen, Gyun Min Lee, Helene Faustrup KildegaardAbstract:Mammalian cells are widely used to express genes for basic biology studies and biopharmaceuticals. Current methods for generation of engineered cell lines introduce high genomic and phenotypic diversity, which hamper studies of gene functions and discovery of novel cellular mechanisms. Here, we minimized Clonal Variation by integrating a landing pad for recombinase-mediated cassette exchange site-specifically into the genome of CHO cells using CRISPR and generated subclones expressing four different recombinant proteins. The subclones showed low Clonal Variation with high consistency in growth, transgene transcript levels and global transcriptional response to recombinant protein expression, enabling improved studies of the impact of transgenes on the host transcriptome. Little Variation over time in subclone phenotypes and transcriptomes was observed when controlling environmental culture conditions. The platform enables robust comparative studies of genome engineered CHO cell lines and can be applied to...
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Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation
2018Co-Authors: Lise Marie Grav, Lars K. Nielsen, Daria Sergeeva, Jae Seong Lee, Igor Marin De Mas, Nathan E Lewis, Mikael Rordam Andersen, Gyun Min Lee, Helene Faustrup KildegaardAbstract:Mammalian cells are widely used to express genes for basic biology studies and biopharmaceuticals. Current methods for generation of engineered cell lines introduce high genomic and phenotypic diversity, which hamper studies of gene functions and discovery of novel cellular mechanisms. Here, we minimized Clonal Variation by integrating a landing pad for recombinase-mediated cassette exchange site-specifically into the genome of CHO cells using CRISPR and generated subclones expressing four different recombinant proteins. The subclones showed low Clonal Variation with high consistency in growth, transgene transcript levels and global transcriptional response to recombinant protein expression, enabling improved studies of the impact of transgenes on the host transcriptome. Little Variation over time in subclone phenotypes and transcriptomes was observed when controlling environmental culture conditions. The platform enables robust comparative studies of genome engineered CHO cell lines and can be applied to other mammalian cells for diverse biological, biomedical and biotechnological applications
L De Temmerman - One of the best experts on this subject based on the ideXlab platform.
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Clonal Variation in heavy metal accumulation and biomass production in a poplar coppice culture ii vertical distribution and phytoextraction potential
Environmental Pollution, 2005Co-Authors: I Laureysens, L De Temmerman, T Hastir, M Van Gysel, R CeulemansAbstract:Short rotation coppice cultures (SRC) are intensively managed, high-density plantations of multi-shoot trees. In April 1996, an SRC field trial with 17 different poplar clones was established in Boom (Belgium) on a former waste disposal site. In December 1996 and January 2001, all shoots were cut back to a height of 5 cm to create a coppice culture. For six clones, wood and bark were sampled at the bottom, middle and top of a shoot in August and November 2002. No significant height effect of metal concentration was found, but for wood, metal concentrations generally increased toward the top of the shoot in August, and decreased toward the top of the shoot in November. Phytoextraction potential of a clone was primarily determined by metal concentration and by biomass production. Shoot size and number of shoots per stool were less important, as a high biomass production could be achieved by producing a few large shoots or many smaller shoots. Clone Fritzi Pauley accumulated 1.4 kg ha(-1) of Al over two years; Wolterson and Balsam Spire showed a relatively high accumulation of Cd and Zn, i.e. averaging, respectively 47 and 57 g ha(-1) for Cd and 2.4 and 2.0 kg ha(-1) for Zn over two years.
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Clonal Variation in heavy metal accumulation and biomass production in a poplar coppice culture i seasonal Variation in leaf wood and bark concentrations
Environmental Pollution, 2004Co-Authors: I Laureysens, L De Temmerman, Ronny Blust, Cmhm Lemmens, R CeulemansAbstract:The use of plants to decontaminate soils polluted by heavy metals has received considerable attention in recent years as a low-cost technique. Poplars (Populus spp.) can accumulate relatively high levels of certain metals, and have the added advantage of producing biomass that can be used for energy production. A short rotation coppice culture with 13 poplar clones was established on a former waste disposal site, which was moderately polluted with heavy metals. Total content of metals in leaves, wood and bark were determined in August and October/November. Significant Clonal differences in accumulation were found for most metals, although clones with the highest concentration of all metals were not found. Cadmium, zinc and aluminium were most efficiently taken up. The lowest concentration was found in wood; the highest concentrations were generally found in senescing leaves, making removal and treatment of fallen leaves necessary.
Francois Lefevre - One of the best experts on this subject based on the ideXlab platform.
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a single gene cluster controls incompatibility and partial resistance to various melampsora larici populina races in hybrid poplars
Phytopathology, 1998Co-Authors: Francois Lefevre, M C Gouemourier, Patricia Faivrerampant, Marc VillarAbstract:ABSTRACT Complete cosegregation for race-specific incompatibility with three Melampsora larici-populina rust races was observed in five F1 hybrid progenies of Populus, with different patterns among the various progenies. A single gene cluster could explain these segregations: one locus with multiple alleles or two tightly linked loci controlling complete resistance to E1 and E3, and two tightly linked loci for E2. The random amplified polymorphic DNA marker OPM03/04_480 was linked to that cluster in all families (<1 cM). This marker accounted for more than 70% of the genetic Variation for field resistance in each family (heritability ≈ 0.40). The same marker accounted for up to 64% of the Clonal Variation for growth in the nursery under natural inoculum pressure; the weak tolerance to rust of F1 interspecific hybrids was attributed to a genetic background effect. Partial resistance was split into epidemiological components (heritability ranged from 0.35 to 0.87). Genotypic correlations among resistance tr...
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intra and interspecific inheritance of some components of the resistance to leaf rust melampsora larici populina kleb in poplars
Theoretical and Applied Genetics, 1994Co-Authors: Francois Lefevre, Christian Pichot, J PinonAbstract:Clonal Variation for rust resistance was studied in a factorial mating design of Populus trichocarpa and P. deltoides with 17 intraspecific and 15 interspecific progenies. Susceptibility to Melampsora larici-populina was assessed both under natural conditions in the nursery and after leaf-disk inoculation with two pathogen races in a controlled environment. Different components of the resistance were taken into account: immunity in the field or in the laboratory, latent period, number and size of uredia in the laboratory, and field resistance at the end of the growing season. Genes controlling immunity were contributed only by the P. deltoides parents. The distributions of Clonal means within each family suggested a polygenic inheritance of field resistance in the P. trichocarpa x P. trichocarpa crosses, but major gene effects were suspected in the interspecific progenies. Inefficiency of the quantitative mechanisms of resistance in the interspecific F1 hybrids might have important implications for future breeding strategies. Field and laboratory trials complemented each other well, and a combined selection approach is proposed.