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Rainer Bischoff - One of the best experts on this subject based on the ideXlab platform.

  • Influence of Clotting Time on the protein composition of serum samples based on LC-MS data
    Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2009
    Co-Authors: Natalia I Govorukhina, Ate G.j. Van Der Zee, Marcel De Vries, Peter Horvatovich, Theo H Reijmers, Rainer Bischoff
    Abstract:

    Many large, disease-related biobanks of serum samples have been established prior to the widespread use of proteomics in biomarker research. These biobanks may contain relevant information about the disease process, response to therapy or patient classifications especially with respect to long-term follow-up that is otherwise very difficult to obtain based on newly initiated studies, particularly in the case of slowly developing diseases. An important parameter that may influence the composition of serum but that is often not exactly known is Clotting Time. We therefore investigated the influence of Clotting Time on the protein and peptide composition of serum by label-free and stable-isotope labeling techniques. The label-free analysis of trypsin-digested serum showed that the overall pattern of LC-MS data is not affected by Clotting Times varying from 2 to 8 h. However, univariate and multivariate statistical analyses revealed that proteins that are directly involved in blood clot formation, such as the Clotting-derived fibrinopeptides, change significantly. This is most easily detected in the supernatant of acid-precipitated, immunodepleted serum. Stable-isotope labeling techniques show that truncated or phosphorylated forms of fibrinopeptides A and B increase or decrease depending on Clotting Time. These patterns can be easily recognized and should be taken into consideration when analyzing LC-MS data using serum sample collections of which the Clotting Time is not known. Next to the fibrinopeptides, leucine-rich alpha-2-glycoprotein (P02750) was shown to be consistently decreased in samples with Clotting Times of more than 1 h. For prospective studies, we recommend to let blood clot for at least 2 h at room temperature using glass tubes with a separation gel and micronized silica to accelerate blood Clotting. © 2008 Elsevier B.V. All rights reserved.

  • Influence of Clotting Time on the protein composition of serum samples based on LC-MS data
    Journal of Chromatography B, 2008
    Co-Authors: Natalia I Govorukhina, Marcel De Vries, Peter Horvatovich, Theo H Reijmers, Rainer Bischoff
    Abstract:

    Abstract Many large, disease-related biobanks of serum samples have been established prior to the widespread use of proteomics in biomarker research. These biobanks may contain relevant information about the disease process, response to therapy or patient classifications especially with respect to long-term follow-up that is otherwise very difficult to obtain based on newly initiated studies, particularly in the case of slowly developing diseases. An important parameter that may influence the composition of serum but that is often not exactly known is Clotting Time. We therefore investigated the influence of Clotting Time on the protein and peptide composition of serum by label-free and stable-isotope labeling techniques. The label-free analysis of trypsin-digested serum showed that the overall pattern of LC–MS data is not affected by Clotting Times varying from 2 to 8 h. However, univariate and multivariate statistical analyses revealed that proteins that are directly involved in blood clot formation, such as the Clotting-derived fibrinopeptides, change significantly. This is most easily detected in the supernatant of acid-precipitated, immunodepleted serum. Stable-isotope labeling techniques show that truncated or phosphorylated forms of fibrinopeptides A and B increase or decrease depending on Clotting Time. These patterns can be easily recognized and should be taken into consideration when analyzing LC–MS data using serum sample collections of which the Clotting Time is not known. Next to the fibrinopeptides, leucine-rich alpha-2-glycoprotein (P02750) was shown to be consistently decreased in samples with Clotting Times of more than 1 h. For prospective studies, we recommend to let blood clot for at least 2 h at room temperature using glass tubes with a separation gel and micronized silica to accelerate blood Clotting.

Natalia I Govorukhina - One of the best experts on this subject based on the ideXlab platform.

  • Influence of Clotting Time on the protein composition of serum samples based on LC-MS data
    Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2009
    Co-Authors: Natalia I Govorukhina, Ate G.j. Van Der Zee, Marcel De Vries, Peter Horvatovich, Theo H Reijmers, Rainer Bischoff
    Abstract:

    Many large, disease-related biobanks of serum samples have been established prior to the widespread use of proteomics in biomarker research. These biobanks may contain relevant information about the disease process, response to therapy or patient classifications especially with respect to long-term follow-up that is otherwise very difficult to obtain based on newly initiated studies, particularly in the case of slowly developing diseases. An important parameter that may influence the composition of serum but that is often not exactly known is Clotting Time. We therefore investigated the influence of Clotting Time on the protein and peptide composition of serum by label-free and stable-isotope labeling techniques. The label-free analysis of trypsin-digested serum showed that the overall pattern of LC-MS data is not affected by Clotting Times varying from 2 to 8 h. However, univariate and multivariate statistical analyses revealed that proteins that are directly involved in blood clot formation, such as the Clotting-derived fibrinopeptides, change significantly. This is most easily detected in the supernatant of acid-precipitated, immunodepleted serum. Stable-isotope labeling techniques show that truncated or phosphorylated forms of fibrinopeptides A and B increase or decrease depending on Clotting Time. These patterns can be easily recognized and should be taken into consideration when analyzing LC-MS data using serum sample collections of which the Clotting Time is not known. Next to the fibrinopeptides, leucine-rich alpha-2-glycoprotein (P02750) was shown to be consistently decreased in samples with Clotting Times of more than 1 h. For prospective studies, we recommend to let blood clot for at least 2 h at room temperature using glass tubes with a separation gel and micronized silica to accelerate blood Clotting. © 2008 Elsevier B.V. All rights reserved.

  • Influence of Clotting Time on the protein composition of serum samples based on LC-MS data
    Journal of Chromatography B, 2008
    Co-Authors: Natalia I Govorukhina, Marcel De Vries, Peter Horvatovich, Theo H Reijmers, Rainer Bischoff
    Abstract:

    Abstract Many large, disease-related biobanks of serum samples have been established prior to the widespread use of proteomics in biomarker research. These biobanks may contain relevant information about the disease process, response to therapy or patient classifications especially with respect to long-term follow-up that is otherwise very difficult to obtain based on newly initiated studies, particularly in the case of slowly developing diseases. An important parameter that may influence the composition of serum but that is often not exactly known is Clotting Time. We therefore investigated the influence of Clotting Time on the protein and peptide composition of serum by label-free and stable-isotope labeling techniques. The label-free analysis of trypsin-digested serum showed that the overall pattern of LC–MS data is not affected by Clotting Times varying from 2 to 8 h. However, univariate and multivariate statistical analyses revealed that proteins that are directly involved in blood clot formation, such as the Clotting-derived fibrinopeptides, change significantly. This is most easily detected in the supernatant of acid-precipitated, immunodepleted serum. Stable-isotope labeling techniques show that truncated or phosphorylated forms of fibrinopeptides A and B increase or decrease depending on Clotting Time. These patterns can be easily recognized and should be taken into consideration when analyzing LC–MS data using serum sample collections of which the Clotting Time is not known. Next to the fibrinopeptides, leucine-rich alpha-2-glycoprotein (P02750) was shown to be consistently decreased in samples with Clotting Times of more than 1 h. For prospective studies, we recommend to let blood clot for at least 2 h at room temperature using glass tubes with a separation gel and micronized silica to accelerate blood Clotting.

Francis Colardyn - One of the best experts on this subject based on the ideXlab platform.

Susan J. Westley - One of the best experts on this subject based on the ideXlab platform.

  • Correlation between Activated Clotting Time and Activated Partial Thromboplastin Times
    Annals of Pharmacotherapy, 2016
    Co-Authors: Maureen A. Smythe, John M. Koerber, Sandra N. Nowak, Joan C. Mattson, Robert L Begle, Susan J. Westley, Mamtha Balasubramaniam
    Abstract:

    OBJECTIVE:To evaluate the correlation between Clotting Time tests and heparin concentration, the correlation between activated Clotting Time (ACT) and activated partial thromboplastin Time (aPTT) results, and to compare the clinical decisions based on ACT results with those based on aPTT results.METHODS:Retrospective evaluation of a large database containing heparin concentrations, ACT results (1 device), and aPTT results (3 different instruments: 2 bedside, 1 laboratory-based). Correlations between heparin concentrations and Clotting Time tests and between ACT results and aPTT results were determined. Clinical decisions regarding heparin dosage adjustments based on ACT results were compared with those based on aPTT results.RESULTS:Correlations between Clotting Time tests and heparin concentrations were r = 0.72 for ACT and r = 0.74–0.86 for the aPTT instruments. The laboratory-based aPTT had the highest correlation to heparin concentrations. The correlation between ACT and aPTT results ranged from r = 0....

  • correlation of activated Clotting Time and activated partial thromboplastin Time to plasma heparin concentration
    Pharmacotherapy, 1999
    Co-Authors: John M. Koerber, Maureen A. Smythe, Joan C. Mattson, Robert L Begle, Beverly P Kershaw, Susan J. Westley
    Abstract:

    Study Objective. To determine the correlation between activated Clotting Time (ACT) or activated partial thromboplastin Time (aPTT) and plasma heparin concentration. Design. Two-phase prospective study. Setting. University-affiliated community hospital. Patients. Thirty patients receiving continuous-infusion intravenous heparin. Interventions. Measurement of ACT, aPTT and plasma heparin concentrations. Measurements and Main Results. Linear and log linear correlations were determined between Clotting Time tests and heparin concentrations. Linear correlations yielded r values of 0.58 for ACT (p=0.008) and 0.89 for aPTT (p=0.0001). Log linear correlations yielded r values of 0.60 for ACT (p=0.005) and 0.88 for aPTT (p=0.0001). A decision analysis was performed to determine possible consequences of dosage adjustments based on either test in relationship to the decision based on plasma heparin concentration. The decision analysis based on ACT disagreed with corresponding decisions based on plasma heparin concentration in 15 of 30 patients; 13 disagreements may have increased the risk of bleeding, and the other 2 may have increased the risk of thrombosis. Decisions based on aPTT disagreed with corresponding decisions based on plasma heparin concentration in 13 of 30 patients; 2 disagreements may have increased the risk of bleeding, and the other 11 may have increased the risk of thrombosis. Conclusion. There are significant statistical linear and log linear correlations between both Clotting Time tests and plasma heparin concentrations, with aPTT showing stronger correlation than ACT. However, decisions regarding heparin therapy based on ACT may increase a patient's risk of bleeding, whereas decisions based on aPTT may increase the risk of thrombus progression or rethrombosis.

Marcel De Vries - One of the best experts on this subject based on the ideXlab platform.

  • Influence of Clotting Time on the protein composition of serum samples based on LC-MS data
    Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2009
    Co-Authors: Natalia I Govorukhina, Ate G.j. Van Der Zee, Marcel De Vries, Peter Horvatovich, Theo H Reijmers, Rainer Bischoff
    Abstract:

    Many large, disease-related biobanks of serum samples have been established prior to the widespread use of proteomics in biomarker research. These biobanks may contain relevant information about the disease process, response to therapy or patient classifications especially with respect to long-term follow-up that is otherwise very difficult to obtain based on newly initiated studies, particularly in the case of slowly developing diseases. An important parameter that may influence the composition of serum but that is often not exactly known is Clotting Time. We therefore investigated the influence of Clotting Time on the protein and peptide composition of serum by label-free and stable-isotope labeling techniques. The label-free analysis of trypsin-digested serum showed that the overall pattern of LC-MS data is not affected by Clotting Times varying from 2 to 8 h. However, univariate and multivariate statistical analyses revealed that proteins that are directly involved in blood clot formation, such as the Clotting-derived fibrinopeptides, change significantly. This is most easily detected in the supernatant of acid-precipitated, immunodepleted serum. Stable-isotope labeling techniques show that truncated or phosphorylated forms of fibrinopeptides A and B increase or decrease depending on Clotting Time. These patterns can be easily recognized and should be taken into consideration when analyzing LC-MS data using serum sample collections of which the Clotting Time is not known. Next to the fibrinopeptides, leucine-rich alpha-2-glycoprotein (P02750) was shown to be consistently decreased in samples with Clotting Times of more than 1 h. For prospective studies, we recommend to let blood clot for at least 2 h at room temperature using glass tubes with a separation gel and micronized silica to accelerate blood Clotting. © 2008 Elsevier B.V. All rights reserved.

  • Influence of Clotting Time on the protein composition of serum samples based on LC-MS data
    Journal of Chromatography B, 2008
    Co-Authors: Natalia I Govorukhina, Marcel De Vries, Peter Horvatovich, Theo H Reijmers, Rainer Bischoff
    Abstract:

    Abstract Many large, disease-related biobanks of serum samples have been established prior to the widespread use of proteomics in biomarker research. These biobanks may contain relevant information about the disease process, response to therapy or patient classifications especially with respect to long-term follow-up that is otherwise very difficult to obtain based on newly initiated studies, particularly in the case of slowly developing diseases. An important parameter that may influence the composition of serum but that is often not exactly known is Clotting Time. We therefore investigated the influence of Clotting Time on the protein and peptide composition of serum by label-free and stable-isotope labeling techniques. The label-free analysis of trypsin-digested serum showed that the overall pattern of LC–MS data is not affected by Clotting Times varying from 2 to 8 h. However, univariate and multivariate statistical analyses revealed that proteins that are directly involved in blood clot formation, such as the Clotting-derived fibrinopeptides, change significantly. This is most easily detected in the supernatant of acid-precipitated, immunodepleted serum. Stable-isotope labeling techniques show that truncated or phosphorylated forms of fibrinopeptides A and B increase or decrease depending on Clotting Time. These patterns can be easily recognized and should be taken into consideration when analyzing LC–MS data using serum sample collections of which the Clotting Time is not known. Next to the fibrinopeptides, leucine-rich alpha-2-glycoprotein (P02750) was shown to be consistently decreased in samples with Clotting Times of more than 1 h. For prospective studies, we recommend to let blood clot for at least 2 h at room temperature using glass tubes with a separation gel and micronized silica to accelerate blood Clotting.