The Experts below are selected from a list of 3363 Experts worldwide ranked by ideXlab platform
Graham A R Johnston - One of the best experts on this subject based on the ideXlab platform.
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CNQX binding to non nmda glutamate receptors in canine cerebro cortical crude synaptosomal membranes pharmacological characterization and comparison of binding parameters in dogs with congenital portosystemic encephalopathy and control dogs
Metabolic Brain Disease, 1992Co-Authors: J E Maddison, Wendy E J Watson, Graham A R JohnstonAbstract:The pharmacological profile and binding characteristics of the non-NMDA antagonist of glutamate receptors [3H]6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX), were investigated in triton-washed crude synaptosomal membranes prepared from canine cerebral cortex. [3H]CNQX binding was inhibited by various glutamate agonists and antagonists, the rank order of potency being CNQX>α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)=quisqualate=kainate>glutamate. Two binding sites for [3H]CNQX were apparent when non-specific binding (NSB) was defined with unlabelled CNQX. In contrast, when NSB was defined with saturating concentrations of unlabelled AMPA and kainate, only one binding site was identified which corresponded to the high affinity site identified when CNQX was used to define NSB. No physiologically relevant differences were found in binding parameters for [3H]CNQX membranes from dogs with congenital portosystemic encephalopathy (PSE) when compared with control dogs. The affinity constant (Ki of AMPA displacement of [3H]CNQX binding was not significantly different in PSE dogs compared with control dogs. These results suggest that the antagonist site on cortical non-NMDA receptors is not perturbed in dogs with congenital PSE.
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CNQX binding to non nmda glutamate receptors in canine cerebro cortical crude synaptosomal membranes pharmacological characterization and comparison of binding parameters in dogs with congenital portosystemic encephalopathy and control dogs
Metabolic Brain Disease, 1992Co-Authors: J E Maddison, Wendy E J Watson, Graham A R JohnstonAbstract:The pharmacological profile and binding characteristics of the non-NMDA antagonist of glutamate receptors [3H]6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX), were investigated in triton-washed crude synaptosomal membranes prepared from canine cerebral cortex. [3H]CNQX binding was inhibited by various glutamate agonists and antagonists, the rank order of potency being CNQX greater than alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) = quisqualate = kainate greater than glutamate. Two binding sites for [3H]CNQX were apparent when non-specific binding (NSB) was defined with unlabelled CNQX. In contrast, when NSB was defined with saturating concentrations of unlabelled AMPA and kainate, only one binding site was identified which corresponded to the high affinity site identified when CNQX was used to define NSB. No physiologically relevant differences were found in binding parameters for [3H]CNQX membranes from dogs with congenital portosystemic encephalopathy (PSE) when compared with control dogs. The affinity constant (Ki) of AMPA displacement of [3H]CNQX binding was not significantly different in PSE dogs compared with control dogs. These results suggest that the antagonist site on cortical non-NMDA receptors is not perturbed in dogs with congenital PSE.
J E Maddison - One of the best experts on this subject based on the ideXlab platform.
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CNQX binding to non nmda glutamate receptors in canine cerebro cortical crude synaptosomal membranes pharmacological characterization and comparison of binding parameters in dogs with congenital portosystemic encephalopathy and control dogs
Metabolic Brain Disease, 1992Co-Authors: J E Maddison, Wendy E J Watson, Graham A R JohnstonAbstract:The pharmacological profile and binding characteristics of the non-NMDA antagonist of glutamate receptors [3H]6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX), were investigated in triton-washed crude synaptosomal membranes prepared from canine cerebral cortex. [3H]CNQX binding was inhibited by various glutamate agonists and antagonists, the rank order of potency being CNQX>α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)=quisqualate=kainate>glutamate. Two binding sites for [3H]CNQX were apparent when non-specific binding (NSB) was defined with unlabelled CNQX. In contrast, when NSB was defined with saturating concentrations of unlabelled AMPA and kainate, only one binding site was identified which corresponded to the high affinity site identified when CNQX was used to define NSB. No physiologically relevant differences were found in binding parameters for [3H]CNQX membranes from dogs with congenital portosystemic encephalopathy (PSE) when compared with control dogs. The affinity constant (Ki of AMPA displacement of [3H]CNQX binding was not significantly different in PSE dogs compared with control dogs. These results suggest that the antagonist site on cortical non-NMDA receptors is not perturbed in dogs with congenital PSE.
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CNQX binding to non nmda glutamate receptors in canine cerebro cortical crude synaptosomal membranes pharmacological characterization and comparison of binding parameters in dogs with congenital portosystemic encephalopathy and control dogs
Metabolic Brain Disease, 1992Co-Authors: J E Maddison, Wendy E J Watson, Graham A R JohnstonAbstract:The pharmacological profile and binding characteristics of the non-NMDA antagonist of glutamate receptors [3H]6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX), were investigated in triton-washed crude synaptosomal membranes prepared from canine cerebral cortex. [3H]CNQX binding was inhibited by various glutamate agonists and antagonists, the rank order of potency being CNQX greater than alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) = quisqualate = kainate greater than glutamate. Two binding sites for [3H]CNQX were apparent when non-specific binding (NSB) was defined with unlabelled CNQX. In contrast, when NSB was defined with saturating concentrations of unlabelled AMPA and kainate, only one binding site was identified which corresponded to the high affinity site identified when CNQX was used to define NSB. No physiologically relevant differences were found in binding parameters for [3H]CNQX membranes from dogs with congenital portosystemic encephalopathy (PSE) when compared with control dogs. The affinity constant (Ki) of AMPA displacement of [3H]CNQX binding was not significantly different in PSE dogs compared with control dogs. These results suggest that the antagonist site on cortical non-NMDA receptors is not perturbed in dogs with congenital PSE.
Chifuyu Takeshige - One of the best experts on this subject based on the ideXlab platform.
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inhibitory effect of 6 cyano 7 nitroquinoxaline 2 3 dione CNQX on the micturition reflex in rat
Neuroscience Letters, 1991Co-Authors: G Matsumoto, Masahito Kawatani, Chifuyu TakeshigeAbstract:Abstract The effect of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an α-amino-hydroxy-5-methyl-4-isoxazole propionate (AMPA) glutamate receptor antagonist, on bladder contractions was examined under isometric conditions in urethane anesthetized rats. Intravenous administration of CNQX (33 ng–50 μg/kg) inhibited or abolished bladder contraction. Before complete inhibition, the frequency of bladder contractions was reduced without altering the amplitude or duration. Intrathecal administration of CNQX (2 ng/kg–11 μg/kg) similarly inhibited bladder contractions. In contrast, CNQX did not affect bladder contractions in chronically spinalized animals (6.7 ng/kg–400 μg/kg i.v.), or contractions evoked by stimulation of the decentralized pelvic nerve (1–100 μg/kg i.v.).
Wendy E J Watson - One of the best experts on this subject based on the ideXlab platform.
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CNQX binding to non nmda glutamate receptors in canine cerebro cortical crude synaptosomal membranes pharmacological characterization and comparison of binding parameters in dogs with congenital portosystemic encephalopathy and control dogs
Metabolic Brain Disease, 1992Co-Authors: J E Maddison, Wendy E J Watson, Graham A R JohnstonAbstract:The pharmacological profile and binding characteristics of the non-NMDA antagonist of glutamate receptors [3H]6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX), were investigated in triton-washed crude synaptosomal membranes prepared from canine cerebral cortex. [3H]CNQX binding was inhibited by various glutamate agonists and antagonists, the rank order of potency being CNQX>α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)=quisqualate=kainate>glutamate. Two binding sites for [3H]CNQX were apparent when non-specific binding (NSB) was defined with unlabelled CNQX. In contrast, when NSB was defined with saturating concentrations of unlabelled AMPA and kainate, only one binding site was identified which corresponded to the high affinity site identified when CNQX was used to define NSB. No physiologically relevant differences were found in binding parameters for [3H]CNQX membranes from dogs with congenital portosystemic encephalopathy (PSE) when compared with control dogs. The affinity constant (Ki of AMPA displacement of [3H]CNQX binding was not significantly different in PSE dogs compared with control dogs. These results suggest that the antagonist site on cortical non-NMDA receptors is not perturbed in dogs with congenital PSE.
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CNQX binding to non nmda glutamate receptors in canine cerebro cortical crude synaptosomal membranes pharmacological characterization and comparison of binding parameters in dogs with congenital portosystemic encephalopathy and control dogs
Metabolic Brain Disease, 1992Co-Authors: J E Maddison, Wendy E J Watson, Graham A R JohnstonAbstract:The pharmacological profile and binding characteristics of the non-NMDA antagonist of glutamate receptors [3H]6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX), were investigated in triton-washed crude synaptosomal membranes prepared from canine cerebral cortex. [3H]CNQX binding was inhibited by various glutamate agonists and antagonists, the rank order of potency being CNQX greater than alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) = quisqualate = kainate greater than glutamate. Two binding sites for [3H]CNQX were apparent when non-specific binding (NSB) was defined with unlabelled CNQX. In contrast, when NSB was defined with saturating concentrations of unlabelled AMPA and kainate, only one binding site was identified which corresponded to the high affinity site identified when CNQX was used to define NSB. No physiologically relevant differences were found in binding parameters for [3H]CNQX membranes from dogs with congenital portosystemic encephalopathy (PSE) when compared with control dogs. The affinity constant (Ki) of AMPA displacement of [3H]CNQX binding was not significantly different in PSE dogs compared with control dogs. These results suggest that the antagonist site on cortical non-NMDA receptors is not perturbed in dogs with congenital PSE.
Masahito Kawatani - One of the best experts on this subject based on the ideXlab platform.
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inhibitory effect of 6 cyano 7 nitroquinoxaline 2 3 dione CNQX on the micturition reflex in rat
Neuroscience Letters, 1991Co-Authors: G Matsumoto, Masahito Kawatani, Chifuyu TakeshigeAbstract:Abstract The effect of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an α-amino-hydroxy-5-methyl-4-isoxazole propionate (AMPA) glutamate receptor antagonist, on bladder contractions was examined under isometric conditions in urethane anesthetized rats. Intravenous administration of CNQX (33 ng–50 μg/kg) inhibited or abolished bladder contraction. Before complete inhibition, the frequency of bladder contractions was reduced without altering the amplitude or duration. Intrathecal administration of CNQX (2 ng/kg–11 μg/kg) similarly inhibited bladder contractions. In contrast, CNQX did not affect bladder contractions in chronically spinalized animals (6.7 ng/kg–400 μg/kg i.v.), or contractions evoked by stimulation of the decentralized pelvic nerve (1–100 μg/kg i.v.).