The Experts below are selected from a list of 59214 Experts worldwide ranked by ideXlab platform
Kunihito Gotoh - One of the best experts on this subject based on the ideXlab platform.
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layer by layer cell Coating Technique using extracellular matrix facilitates rapid fabrication and function of pancreatic β cell spheroids
Biomaterials, 2018Co-Authors: Yasunari Fukuda, Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Koichi Kawamoto, Kunihito GotohAbstract:Tissue engineering of insulin-secreting cells using alternatives to islet transplantation has been fueled by the development of available materials and fabrication Techniques. We have established a cell Coating Technique that enables the cell surface to be coated with extracellular matrix based on the concept of a layer-by-layer (LbL) assembly. The present study evaluated whether this Technique is beneficial for fabricating pancreatic β-cell spheroids using a mouse β-cell line. The well-structured and dense spheroids could immediately be constructed by the LbL-coated cells. In the functional analysis, spheroids with the LbL-coated cells had greater insulin secretion ability with increased expression of the insulin and glucose transporter 2 genes versus spheroids with non-coated cells. In addition, we found that the expression of connexin 36, a gap junction molecule, was upregulated by the LbL cell Coating. When spheroids with the LbL-coated cells were syngeneically transplanted in diabetic mice, blood glucose levels immediately decreased and glucose sensitivity significantly improved after intraperitoneal glucose stimulation compared to spheroids with non-coated cells. This cell Coating Technique would be a clinically applicable approach for fabricating pancreatic β-cell spheroids and treating type 1 diabetes mellitus.
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construction of three dimensional vascularized functional human liver tissue using a layer by layer cell Coating Technique
Biomaterials, 2017Co-Authors: Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yasunari Fukuda, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hiroshi Wada, Kunihito GotohAbstract:The creation of artificial liver tissue is an active area of research due to the shortage of donors for liver transplantation. Here we investigated whether a simple and efficient cell Coating Technique developed in our laboratory could be used to generate functional vascularized liver tissue. This Technique creates three-dimensional tissue by loading cells sterically onto other cells that have been coated with layer-by-layer (LbL) nanofilms of fibronectin and gelatin, two extracellular matrix proteins. We used this Technique to construct homogenous, dense, well-vascularized liver tissue from cryopreserved human primary hepatocytes, human umbilical vein endothelial cells, and normal human dermal fibroblasts. Using LbL cell Coating Technique resulted in higher cellular function in terms of human albumin production (P < 0.01) and cytochrome P450 activity (P < 0.01) in vitro. Furthermore, after being transplanted subcutaneously into NOD/SCID mice, the vascularized liver tissue showed greater albumin production in the early stage than non-vascularized tissue or a hepatocyte suspension (P < 0.01). Histological examination demonstrated that compare to non-vascularized tissue, there were many less-morphologically changed and intact hepatocytes in the vascularized tissue. This cell Coating Technique would be applicable to the generation of vascularized functional liver tissue for regenerative medicine in the future.
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Construction of three-dimensional vascularized functional human liver tissue using a layer-by-layer cell Coating Technique.
Biomaterials, 2017Co-Authors: Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yasunari Fukuda, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hiroshi Wada, Kunihito GotohAbstract:The creation of artificial liver tissue is an active area of research due to the shortage of donors for liver transplantation. Here we investigated whether a simple and efficient cell Coating Technique developed in our laboratory could be used to generate functional vascularized liver tissue. This Technique creates three-dimensional tissue by loading cells sterically onto other cells that have been coated with layer-by-layer (LbL) nanofilms of fibronectin and gelatin, two extracellular matrix proteins. We used this Technique to construct homogenous, dense, well-vascularized liver tissue from cryopreserved human primary hepatocytes, human umbilical vein endothelial cells, and normal human dermal fibroblasts. Using LbL cell Coating Technique resulted in higher cellular function in terms of human albumin production (P
Tomasz Piotr Nowak - One of the best experts on this subject based on the ideXlab platform.
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Application of photoelastic Coating Technique in tests of solid wooden beams reinforced with CFRP strips
Archives of Civil and Mechanical Engineering, 2012Co-Authors: Tomasz Piotr Nowak, Ludomir J. Jankowski, J. JasieŃkoAbstract:The paper presents selected results of tests carried out on hundred year old joists strengthened with carbon fibre reinforced polymers (CFRP). Besides the conventional electric-resistance extensometers (ERSG), the photoelastic Coating Technique (PCT) was used to measure strains in the reinforced (bonded) cross sections. No such attempt to apply PCT has been described in the literature before. The Technique requires further studies to verify agreement between its results and the ones obtained by conventional measuring Techniques.
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Application of photoelastic Coating Technique in tests of solid wooden beams reinforced with CFRP strips
Archives of Civil and Mechanical Engineering, 2010Co-Authors: Tomasz Piotr Nowak, Ludomir J. Jankowski, J. JasieńkoAbstract:W pracy przedstawiono wybrane wyniki badań wzmocnionych stuletnich belek stropowych. Do wzmocnienia i odtworzenia nośności drewnianych belek z defektami — korozja biologiczna, inkluzje, skręt włókien, pęknięcia drewna — zastosowano taśmy CFRP ( carbon fibre reinforced polymers ). Obok tradycyjnych metod pomiarowych — czujniki elektrooporowe (ERSG) — do pomiaru odkształceń przekrojów wzmocnionych (zespolonych) zastosowano metodę elastooptycznej warstwy powierzchniowej (PCT). Dotychczas nie opisano takiej próby w literaturze przedmiotu. Metoda wymaga przeprowadzenia dalszych badań, mających na celu weryfikację zgodności z pomiarami metodami tradycyjnymi. The paper presents selected results of tests carried out on hundred year old joists strengthened with carbon fibre reinforced polymers (CFRP). Besides the conventional electric-resistance extensometers (ERSG), the photoelastic Coating Technique (PCT) was used to measure strains in the reinforced (bonded) cross sections. No such attempt to apply PCT has been described in the literature before. The Technique requires further studies to verify agreement between its results and the ones obtained by conventional measuring Techniques.
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Experimental assessment of CFRP reinforced wooden beams by 4-point bending tests and photoelastic Coating Technique
Materials and Structures Materiaux et Constructions, 2010Co-Authors: Ludomir J. Jankowski, J. JasieŃko, Tomasz Piotr NowakAbstract:The article presents selected results of experimental investigations into carbon fibre rein-forced plastic (CFRP) strips for strengthening wooden beams. About 100 years old beams were tested under four-point bending load. Electrical-resistance strain gauges (ERSG) together with the photoelastic Coating Technique (PCT) were used to determine strain distributions (also in the inserted strips). A comparison of the strain values measured by, respectively, ERSG and PCT showed significant differences (amounting to 2.2–72.5%) due to the different character of the two kinds of measurement, connected with the significant effect of wood struc-ture on local strain distributions. Generally, the strain measurement results suggest that the proposed methods of reinforcing wooden beams with CFRP strips are useful for restoration purposes. The effec-tiveness of the reinforcement significantly depends on the quality of the wood-CFRP strip bond, which means the preparation of the surfaces for bonding plays an important role.
Kazuki Sasaki - One of the best experts on this subject based on the ideXlab platform.
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layer by layer cell Coating Technique using extracellular matrix facilitates rapid fabrication and function of pancreatic β cell spheroids
Biomaterials, 2018Co-Authors: Yasunari Fukuda, Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Koichi Kawamoto, Kunihito GotohAbstract:Tissue engineering of insulin-secreting cells using alternatives to islet transplantation has been fueled by the development of available materials and fabrication Techniques. We have established a cell Coating Technique that enables the cell surface to be coated with extracellular matrix based on the concept of a layer-by-layer (LbL) assembly. The present study evaluated whether this Technique is beneficial for fabricating pancreatic β-cell spheroids using a mouse β-cell line. The well-structured and dense spheroids could immediately be constructed by the LbL-coated cells. In the functional analysis, spheroids with the LbL-coated cells had greater insulin secretion ability with increased expression of the insulin and glucose transporter 2 genes versus spheroids with non-coated cells. In addition, we found that the expression of connexin 36, a gap junction molecule, was upregulated by the LbL cell Coating. When spheroids with the LbL-coated cells were syngeneically transplanted in diabetic mice, blood glucose levels immediately decreased and glucose sensitivity significantly improved after intraperitoneal glucose stimulation compared to spheroids with non-coated cells. This cell Coating Technique would be a clinically applicable approach for fabricating pancreatic β-cell spheroids and treating type 1 diabetes mellitus.
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construction of three dimensional vascularized functional human liver tissue using a layer by layer cell Coating Technique
Biomaterials, 2017Co-Authors: Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yasunari Fukuda, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hiroshi Wada, Kunihito GotohAbstract:The creation of artificial liver tissue is an active area of research due to the shortage of donors for liver transplantation. Here we investigated whether a simple and efficient cell Coating Technique developed in our laboratory could be used to generate functional vascularized liver tissue. This Technique creates three-dimensional tissue by loading cells sterically onto other cells that have been coated with layer-by-layer (LbL) nanofilms of fibronectin and gelatin, two extracellular matrix proteins. We used this Technique to construct homogenous, dense, well-vascularized liver tissue from cryopreserved human primary hepatocytes, human umbilical vein endothelial cells, and normal human dermal fibroblasts. Using LbL cell Coating Technique resulted in higher cellular function in terms of human albumin production (P < 0.01) and cytochrome P450 activity (P < 0.01) in vitro. Furthermore, after being transplanted subcutaneously into NOD/SCID mice, the vascularized liver tissue showed greater albumin production in the early stage than non-vascularized tissue or a hepatocyte suspension (P < 0.01). Histological examination demonstrated that compare to non-vascularized tissue, there were many less-morphologically changed and intact hepatocytes in the vascularized tissue. This cell Coating Technique would be applicable to the generation of vascularized functional liver tissue for regenerative medicine in the future.
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Construction of three-dimensional vascularized functional human liver tissue using a layer-by-layer cell Coating Technique.
Biomaterials, 2017Co-Authors: Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yasunari Fukuda, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hiroshi Wada, Kunihito GotohAbstract:The creation of artificial liver tissue is an active area of research due to the shortage of donors for liver transplantation. Here we investigated whether a simple and efficient cell Coating Technique developed in our laboratory could be used to generate functional vascularized liver tissue. This Technique creates three-dimensional tissue by loading cells sterically onto other cells that have been coated with layer-by-layer (LbL) nanofilms of fibronectin and gelatin, two extracellular matrix proteins. We used this Technique to construct homogenous, dense, well-vascularized liver tissue from cryopreserved human primary hepatocytes, human umbilical vein endothelial cells, and normal human dermal fibroblasts. Using LbL cell Coating Technique resulted in higher cellular function in terms of human albumin production (P
Yasunari Fukuda - One of the best experts on this subject based on the ideXlab platform.
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layer by layer cell Coating Technique using extracellular matrix facilitates rapid fabrication and function of pancreatic β cell spheroids
Biomaterials, 2018Co-Authors: Yasunari Fukuda, Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Koichi Kawamoto, Kunihito GotohAbstract:Tissue engineering of insulin-secreting cells using alternatives to islet transplantation has been fueled by the development of available materials and fabrication Techniques. We have established a cell Coating Technique that enables the cell surface to be coated with extracellular matrix based on the concept of a layer-by-layer (LbL) assembly. The present study evaluated whether this Technique is beneficial for fabricating pancreatic β-cell spheroids using a mouse β-cell line. The well-structured and dense spheroids could immediately be constructed by the LbL-coated cells. In the functional analysis, spheroids with the LbL-coated cells had greater insulin secretion ability with increased expression of the insulin and glucose transporter 2 genes versus spheroids with non-coated cells. In addition, we found that the expression of connexin 36, a gap junction molecule, was upregulated by the LbL cell Coating. When spheroids with the LbL-coated cells were syngeneically transplanted in diabetic mice, blood glucose levels immediately decreased and glucose sensitivity significantly improved after intraperitoneal glucose stimulation compared to spheroids with non-coated cells. This cell Coating Technique would be a clinically applicable approach for fabricating pancreatic β-cell spheroids and treating type 1 diabetes mellitus.
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construction of three dimensional vascularized functional human liver tissue using a layer by layer cell Coating Technique
Biomaterials, 2017Co-Authors: Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yasunari Fukuda, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hiroshi Wada, Kunihito GotohAbstract:The creation of artificial liver tissue is an active area of research due to the shortage of donors for liver transplantation. Here we investigated whether a simple and efficient cell Coating Technique developed in our laboratory could be used to generate functional vascularized liver tissue. This Technique creates three-dimensional tissue by loading cells sterically onto other cells that have been coated with layer-by-layer (LbL) nanofilms of fibronectin and gelatin, two extracellular matrix proteins. We used this Technique to construct homogenous, dense, well-vascularized liver tissue from cryopreserved human primary hepatocytes, human umbilical vein endothelial cells, and normal human dermal fibroblasts. Using LbL cell Coating Technique resulted in higher cellular function in terms of human albumin production (P < 0.01) and cytochrome P450 activity (P < 0.01) in vitro. Furthermore, after being transplanted subcutaneously into NOD/SCID mice, the vascularized liver tissue showed greater albumin production in the early stage than non-vascularized tissue or a hepatocyte suspension (P < 0.01). Histological examination demonstrated that compare to non-vascularized tissue, there were many less-morphologically changed and intact hepatocytes in the vascularized tissue. This cell Coating Technique would be applicable to the generation of vascularized functional liver tissue for regenerative medicine in the future.
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Construction of three-dimensional vascularized functional human liver tissue using a layer-by-layer cell Coating Technique.
Biomaterials, 2017Co-Authors: Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yasunari Fukuda, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hiroshi Wada, Kunihito GotohAbstract:The creation of artificial liver tissue is an active area of research due to the shortage of donors for liver transplantation. Here we investigated whether a simple and efficient cell Coating Technique developed in our laboratory could be used to generate functional vascularized liver tissue. This Technique creates three-dimensional tissue by loading cells sterically onto other cells that have been coated with layer-by-layer (LbL) nanofilms of fibronectin and gelatin, two extracellular matrix proteins. We used this Technique to construct homogenous, dense, well-vascularized liver tissue from cryopreserved human primary hepatocytes, human umbilical vein endothelial cells, and normal human dermal fibroblasts. Using LbL cell Coating Technique resulted in higher cellular function in terms of human albumin production (P
Hidetoshi Eguchi - One of the best experts on this subject based on the ideXlab platform.
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layer by layer cell Coating Technique using extracellular matrix facilitates rapid fabrication and function of pancreatic β cell spheroids
Biomaterials, 2018Co-Authors: Yasunari Fukuda, Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Koichi Kawamoto, Kunihito GotohAbstract:Tissue engineering of insulin-secreting cells using alternatives to islet transplantation has been fueled by the development of available materials and fabrication Techniques. We have established a cell Coating Technique that enables the cell surface to be coated with extracellular matrix based on the concept of a layer-by-layer (LbL) assembly. The present study evaluated whether this Technique is beneficial for fabricating pancreatic β-cell spheroids using a mouse β-cell line. The well-structured and dense spheroids could immediately be constructed by the LbL-coated cells. In the functional analysis, spheroids with the LbL-coated cells had greater insulin secretion ability with increased expression of the insulin and glucose transporter 2 genes versus spheroids with non-coated cells. In addition, we found that the expression of connexin 36, a gap junction molecule, was upregulated by the LbL cell Coating. When spheroids with the LbL-coated cells were syngeneically transplanted in diabetic mice, blood glucose levels immediately decreased and glucose sensitivity significantly improved after intraperitoneal glucose stimulation compared to spheroids with non-coated cells. This cell Coating Technique would be a clinically applicable approach for fabricating pancreatic β-cell spheroids and treating type 1 diabetes mellitus.
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construction of three dimensional vascularized functional human liver tissue using a layer by layer cell Coating Technique
Biomaterials, 2017Co-Authors: Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yasunari Fukuda, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hiroshi Wada, Kunihito GotohAbstract:The creation of artificial liver tissue is an active area of research due to the shortage of donors for liver transplantation. Here we investigated whether a simple and efficient cell Coating Technique developed in our laboratory could be used to generate functional vascularized liver tissue. This Technique creates three-dimensional tissue by loading cells sterically onto other cells that have been coated with layer-by-layer (LbL) nanofilms of fibronectin and gelatin, two extracellular matrix proteins. We used this Technique to construct homogenous, dense, well-vascularized liver tissue from cryopreserved human primary hepatocytes, human umbilical vein endothelial cells, and normal human dermal fibroblasts. Using LbL cell Coating Technique resulted in higher cellular function in terms of human albumin production (P < 0.01) and cytochrome P450 activity (P < 0.01) in vitro. Furthermore, after being transplanted subcutaneously into NOD/SCID mice, the vascularized liver tissue showed greater albumin production in the early stage than non-vascularized tissue or a hepatocyte suspension (P < 0.01). Histological examination demonstrated that compare to non-vascularized tissue, there were many less-morphologically changed and intact hepatocytes in the vascularized tissue. This cell Coating Technique would be applicable to the generation of vascularized functional liver tissue for regenerative medicine in the future.
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Construction of three-dimensional vascularized functional human liver tissue using a layer-by-layer cell Coating Technique.
Biomaterials, 2017Co-Authors: Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yasunari Fukuda, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hiroshi Wada, Kunihito GotohAbstract:The creation of artificial liver tissue is an active area of research due to the shortage of donors for liver transplantation. Here we investigated whether a simple and efficient cell Coating Technique developed in our laboratory could be used to generate functional vascularized liver tissue. This Technique creates three-dimensional tissue by loading cells sterically onto other cells that have been coated with layer-by-layer (LbL) nanofilms of fibronectin and gelatin, two extracellular matrix proteins. We used this Technique to construct homogenous, dense, well-vascularized liver tissue from cryopreserved human primary hepatocytes, human umbilical vein endothelial cells, and normal human dermal fibroblasts. Using LbL cell Coating Technique resulted in higher cellular function in terms of human albumin production (P