The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Blasi Elisabetta - One of the best experts on this subject based on the ideXlab platform.
-
The protein \u201cmycoarray\u201d: a novel serological assay for the laboratory diagnosis of primitive endemic mycoses
2011Co-Authors: Ardizzoni Andrea, Baschieri, Maria Cristina, Manca Lidia, Orsi, Carlotta Francesca, C. Venturelli, M. Meacci, Peppoloni Samuele, C. Farina, Blasi ElisabettaAbstract:A protein microarray containing fungal antigens (the \u201cmycoarray\u201d) has been set up to provide rapid and appropriateserodiagnosis of primitive endemic mycoses, an important cause of morbidity and mortality in an increasingly highnumber of patients. The mycoarray consists of three antigen extracts (histoplasmin, Coccidioidin and Coccidioides \u201cTP\u201d)and antibody dilution curves were spotted on microarray slides. The arrays were processed with coccidioidomycosisand histoplasmosis patients\u2019 sera or with control sera and the occurring immunocomplexes were detected by indirectimmunofluorescence. In agreement with clinical and microbiological diagnosis, the results distinguished betweenhistoplasmosis and coccidioidomycosis patients. In addition, the assay could clearly discriminate between IgM andIgG antibody reactivity. No reactivity was ever observed in the arrays processed with negative control sera. Therefore,this pilot study demonstrates that the \u201cmycoarray\u201d is sensitive and specific enough to discriminate between healthyindividuals and patients with histoplasmosis or coccidioidomycosis. Because of miniaturization and multiparametricity,the new assay cuts costs and processing time. Thus, once clinically validated and implemented as a large-scale array,the \u201cmycoarray\u201d will be ready to be applied to the daily clinical practice
-
The protein “mycoarray”: a novel serological assay for the laboratory diagnosis of primitive endemic mycoses
2011Co-Authors: Ardizzoni Andrea, Baschieri, Maria Cristina, Manca Lidia, Orsi, Carlotta Francesca, C. Venturelli, M. Meacci, Peppoloni Samuele, C. Farina, Blasi ElisabettaAbstract:A protein microarray containing fungal antigens (the \u201cmycoarray\u201d) has been set up to provide rapid and appropriateserodiagnosis of primitive endemic mycoses, an important cause of morbidity and mortality in an increasingly highnumber of patients. The mycoarray consists of three antigen extracts (histoplasmin, Coccidioidin and Coccidioides \u201cTP\u201d)and antibody dilution curves were spotted on microarray slides. The arrays were processed with coccidioidomycosisand histoplasmosis patients\u2019 sera or with control sera and the occurring immunocomplexes were detected by indirectimmunofluorescence. In agreement with clinical and microbiological diagnosis, the results distinguished betweenhistoplasmosis and coccidioidomycosis patients. In addition, the assay could clearly discriminate between IgM andIgG antibody reactivity. No reactivity was ever observed in the arrays processed with negative control sera. Therefore,this pilot study demonstrates that the \u201cmycoarray\u201d is sensitive and specific enough to discriminate between healthyindividuals and patients with histoplasmosis or coccidioidomycosis. Because of miniaturization and multiparametricity,the new assay cuts costs and processing time. Thus, once clinically validated and implemented as a large-scale array,the \u201cmycoarray\u201d will be ready to be applied to the daily clinical practice
-
The protein “mycoarray”: a novel immunoassay for the serological diagnosis of primitive invasive mycoses
ECCMID meeting in Vienna; 10 – 13 April 2010., 2010Co-Authors: Ardizzoni Andrea, Baschieri, Maria Cristina, Manca Lidia, Farina Claudio, Cermelli Claudio, Meacci Marisa, Venturelli Claudia, Blasi ElisabettaAbstract:Objectives. Invasive fungal infections are an important cause of morbidity and mortality in an increasingly higher number of patients, also because of difficulties in providing a rapid and appropriate diagnosis. In some cases, detection of a specific antibody response is a crucial diagnostic tool; however, the available serological assays often provide qualitative results only, their sensitivity and specificity are poor and long time procedures are required. In addition, patients who suffer from an invasive mycosis may have multiple infections likely underestimated by conventional diagnostic approaches. In order to couple the serology of primitive invasive mycoses to the protein microarray technology, a \u201cmycoarray\u201d assay has been designed and set up.Methods. Four antigen extracts (histoplasmin, Coccidioidin, Coccidioides \u201cTP\u201d antigen and aspergillin) and the appropriate controls were spotted in various conditions onto a restricted area of a microscope slide. The printed slides were then incubated with immune sera produced in goat against each single antigen or, subsequently, with human sera (6 from patients affected by primitive invasive mycoses and 7 from healthy individuals). The occurring immunocomplexes were detected by indirect immunofluorescence.Results. The pilot experiments, conducted using the goat immune sera, allowed to establish the optimal spotting conditions for each antigen in terms of both spotting buffer and extracts\u2019 dilution. The \u201cmycoarrays\u201d, obtained by spotting all the fungal antigens with the best condition, were then processed with sera either from patients or control subjects. The reactivity observed in the arrays processed with the patients\u2019 samples was in agreement with the clinical and microbiological diagnosis; no reactivity was ever observed in the arrays processed with the negative control sera.Conclusions. The \u201cprotein mycoarray\u201d is sensitive enough to discriminate between healthy individuals and patients affected by histoplasmosis or coccidioidomycosis. This novel diagnostic tool, because of its intrinsic features, miniaturization and multiparametricity, can contribute to cut out costs and to shorten times-to-results, with the potentiality to be included in the daily clinical practice in the near future
-
The protein \u201cmycoarray\u201d: a novel immunoassay for the serological diagnosis of primitive invasive mycoses
ECCMID meeting in Vienna; 10 \u2013 13 April 2010., 2010Co-Authors: Ardizzoni Andrea, Baschieri, Maria Cristina, Manca Lidia, Farina Claudio, Cermelli Claudio, Meacci Marisa, Venturelli Claudia, Blasi ElisabettaAbstract:Objectives. Invasive fungal infections are an important cause of morbidity and mortality in an increasingly higher number of patients, also because of difficulties in providing a rapid and appropriate diagnosis. In some cases, detection of a specific antibody response is a crucial diagnostic tool; however, the available serological assays often provide qualitative results only, their sensitivity and specificity are poor and long time procedures are required. In addition, patients who suffer from an invasive mycosis may have multiple infections likely underestimated by conventional diagnostic approaches. In order to couple the serology of primitive invasive mycoses to the protein microarray technology, a \u201cmycoarray\u201d assay has been designed and set up.Methods. Four antigen extracts (histoplasmin, Coccidioidin, Coccidioides \u201cTP\u201d antigen and aspergillin) and the appropriate controls were spotted in various conditions onto a restricted area of a microscope slide. The printed slides were then incubated with immune sera produced in goat against each single antigen or, subsequently, with human sera (6 from patients affected by primitive invasive mycoses and 7 from healthy individuals). The occurring immunocomplexes were detected by indirect immunofluorescence.Results. The pilot experiments, conducted using the goat immune sera, allowed to establish the optimal spotting conditions for each antigen in terms of both spotting buffer and extracts\u2019 dilution. The \u201cmycoarrays\u201d, obtained by spotting all the fungal antigens with the best condition, were then processed with sera either from patients or control subjects. The reactivity observed in the arrays processed with the patients\u2019 samples was in agreement with the clinical and microbiological diagnosis; no reactivity was ever observed in the arrays processed with the negative control sera.Conclusions. The \u201cprotein mycoarray\u201d is sensitive enough to discriminate between healthy individuals and patients affected by histoplasmosis or coccidioidomycosis. This novel diagnostic tool, because of its intrinsic features, miniaturization and multiparametricity, can contribute to cut out costs and to shorten times-to-results, with the potentiality to be included in the daily clinical practice in the near future
R. Negroni - One of the best experts on this subject based on the ideXlab platform.
-
Historia del descubrimiento de la coccidioidomicosis History of the discovery of the coccidioidomycosis
Asociación Argentina de Dermatología, 2011Co-Authors: R. NegroniAbstract:La coccidioidomicosis es una micosis sistémica, endémica en las zonas áridas del continente americano, producida por los hongos dimorfos Coccidioides immitis y Coccidioides posadasii. El propósito de esta presentación es dar a conocer la forma en que esta enfermedad y sus agentes causales fueron descubiertos. La enfermedad fue descubierta en 1892, en Buenos Aires, por Posadas y Wernicke. Estos investigadores describieron prolijamente las manifestaciones clínicas de la enfermedad, la histopatología, la fase tisular del agente causal y lograron reproducir la afección en animales. Este estudio fue un modelo de investigación científica para su época. Pensaron que esta nueva enfermedad era debida a un protozoario del género Psorosperma y no consiguieron cultivarlo "in vitro". En1894, Rixford y Gilchrist, en San Francisco (California), observaron un caso similar y pensaron que era debido a un protozoario, al que llamaron Coccidioides immitis. En 1900, Ophüls y Moffit, consiguieron reproducir la enfermedad en cobayos inoculados con de un moho blanquecino, cultivado a partir de las lesiones de pacientes con coccidiodomicosis y comprobaron así la naturaleza fúngica de la infección. Después de este estudio de Ophüls, los investigadoresde California demostraron la existencia de infecciones autolimitadas, la importancia de las pruebas cutáneas y serológicas con Coccidioidina, la vía de infección, los tratamientos eficaces y más recientemente las características genéticas de los agentes causales.Coccidioidomycosis is a systemic mycosis, endemic in arid regions of the American continent; it is due to the dimorphic fungi Coccidiodes immitis and Coccidiodes posadasii. The aim of this presentation is to relate the way in which this mycosis and its etiologic agents were discovered. The disease was reported for the first time in Buenos Aires in 1892, by Posadas and Wernicke. They performed a very skilful research, considered outstanding in those days. They did a very nice description of the clinical manifestations of the chronic disseminated form of coccidioidomycosis, the histopathology of skin and lymph nodes lesions, the microscopic aspects of the etiologic agent in tissues and they were able to reproduce the disease in several animal species. Posadas and Wernicke considered that this infectious disease was due to protozoa of the gender Psorosperma and they could not obtain cultures "in vitro". The first Californian patient suffering this mycosis was reported by Rixford and Gilchrist in San Francisco, in 1894. The patient was a rural worker who had come from San Joaquin Valley. They also thought that the etiologic agent was protozoa and they named this microorganism Coccidiodes immitis. In 1900, Ophüls and Moffit were able to reproduce this disease in guinea pigs by the inoculation with a white mould which had been isolated from patient's lesions. In this way they proved the fungal nature of the etiologic agent of coccidioidomycosis. After Ophüls studies the Californian researchers proved the existence of benign and self limited forms of infection, the diagnostic importance of Coccidioidin skin and serologic tests, the infection portal of entry, the efficacy of different treatments and, more recently the genetic characteristics of the etiologic agents
Neil M Ampel - One of the best experts on this subject based on the ideXlab platform.
-
an analysis of skin test responses to spherulin based Coccidioidin spherusol among a group of subjects with various forms of active coccidioidomycosis
Mycopathologia, 2019Co-Authors: Neil M Ampel, Ian F Robey, Chinh NguyenAbstract:A reformulated skin test for coccidioidomycosis, Spherusol®, was recently approved for use in the USA. We hypothesized that it could be useful in predicting severity of illness and outcome in various types of coccidioidomycosis. Subjects with non-meningeal coccidioidomycosis attending a clinic in the coccidioidal endemic region were skin tested with Spherusol® and clinical data were collected at the time of testing and at follow-up. Twenty-seven subjects were studied, eight of whom had extrathoracic dissemination. A total of 15 subjects had positive tests, including 11 of 19 subjects with non-disseminated pulmonary disease and four with extrathoracic disseminated coccidioidomycosis. Among those with non-disseminated pulmonary disease, age ≥ 65 years, female sex, and antifungal therapy were significantly associated with a negative test on univariate but not multivariate analysis. For 23 subjects, there was a trend for those not on antifungal therapy at the time of follow-up to have a positive test but no association with coccidioidal complement-fixation titer or overall outcome. Not all subjects with non-disseminated pulmonary coccidioidomycosis were found to be skin test positive and half of those with extrathoracic disseminated disease manifested dermal hypersensitivity. In this small study, the results of the skin test were not clinically predictive of disease severity or outcome.
-
A Reformulated Spherule-Derived Coccidioidin (Spherusol) to Detect Delayed-Type Hypersensitivity in Coccidioidomycosis
Mycopathologia, 2012Co-Authors: Royce Johnson, Steven M. Kernerman, Bradley G. Sawtelle, Suresh C. Rastogi, H. Stewart Nielsen, Neil M AmpelAbstract:The ability of spherule-derived Coccidioidin containing 0.4 % phenol and 0.0001 % thimerosal in buffered saline to induce delayed-type hypersensitivity (DTH) was evaluated in four separate studies. The skin test antigen was titrated in 20 adult volunteers with a recent history of pulmonary coccidioidomycosis using intradermal doses of 0.4, 0.8, and 1.6 μg of antigen, based on total dry weight. Based on these data, a dose of 1.27 μg was shown to elicit a mean ± SEM induration response of 23.5 ± 2.3 mm at 48 h, similar to the 23.6-mm response after 48 h of the U. S. Reference Coccidioidin last tested approximately 13 years ago. The 1.27 μg dose in 0.1 mL of the spherule-derived antigen (Spherusol) was then examined in three separate groups of adult volunteers to determine the sensitivity and specificity of the product. Fifty-nine of 60 individuals living in a non-endemic area for coccidioidomycosis were skin test negative to Spherusol. Twelve subjects with a recent history of pulmonary histoplasmosis were skin test negative to Spherusol. Finally, 51 of 52 individuals with a recent diagnosis of acute pulmonary coccidioidomycosis were skin test positive to Spherusol. Within this group, prior therapy with fluconazole did not appear to reduce the reactivity to Spherusol. No serious adverse events were observed in the four studies. From these data, Spherusol was found to be safe and has an overall observed sensitivity and specificity of ≥98 % in detecting DTH in coccidioidomycosis.
-
in vitro whole blood analysis of cellular immunity in patients with active coccidioidomycosis by using the antigen preparation t27k
Clinical and Vaccine Immunology, 2002Co-Authors: Neil M Ampel, Larissa A Kramer, Deborah S Carroll, K M Kerekes, Suzanne M Johnson, Demosthenes PappagianisAbstract:Measurement of cellular immunity in human coccidioidomycosis has important diagnostic and prognostic implications. The Coccidioidin skin test has been the standard for the measurement of this, but it is not available in the United States. We examined the utility of measuring surface expression of CD69 on T lymphocytes in whole blood incubated with the coccidioidal antigen preparation T27K as an alternative to the skin test. Seventy donors with active coccidioidomycosis were studied. The mean fluorescent intensity (MFI) of CD69 expression on CD3 lymphocytes in response to T27K was 28.61 ± 1.77, significantly greater than the control response of 11.45 ± 0.78 (P < 0.001). The MFI CD69 response to T27K above that for the control (MFI CD69 above control) was 6.35 ± 2.18 for seven subjects with disseminated coccidioidomycosis who were studied within 5 months of diagnosis. This was significantly below the value of 20.17 ± 3.17 for 18 subjects with pulmonary coccidioidomycosis studied within 5 months of diagnosis and the value of 19.58 ± 2.91 for 27 subjects with disseminated coccidioidomycosis studied after 5 months of diagnosis (for both, P < 0.05). There was an inverse correlation between coccidioidal clinical score and MFI CD69 above control for all 34 subjects with disseminated coccidioidomycosis (r = 0.362; P = 0.036) but not for the 36 subjects with pulmonary disease (r < 0.001; P = 0.993). Among 30 subjects for whom data were available, there was a highly significant association between the MFI CD69 above control and the supernatant concentrations of gamma interferon, interleukin-2 (IL-2), and tumor necrosis factor alpha (for all, P < 0.001), but not for IL-4, IL-5, or IL-10. These data indicate that in vitro assessment of CD69 expression on T lymphocytes by using T27K may be a useful measure of cellular immune response among subjects with active coccidioidomycosis.
Ardizzoni Andrea - One of the best experts on this subject based on the ideXlab platform.
-
The protein \u201cmycoarray\u201d: a novel serological assay for the laboratory diagnosis of primitive endemic mycoses
2011Co-Authors: Ardizzoni Andrea, Baschieri, Maria Cristina, Manca Lidia, Orsi, Carlotta Francesca, C. Venturelli, M. Meacci, Peppoloni Samuele, C. Farina, Blasi ElisabettaAbstract:A protein microarray containing fungal antigens (the \u201cmycoarray\u201d) has been set up to provide rapid and appropriateserodiagnosis of primitive endemic mycoses, an important cause of morbidity and mortality in an increasingly highnumber of patients. The mycoarray consists of three antigen extracts (histoplasmin, Coccidioidin and Coccidioides \u201cTP\u201d)and antibody dilution curves were spotted on microarray slides. The arrays were processed with coccidioidomycosisand histoplasmosis patients\u2019 sera or with control sera and the occurring immunocomplexes were detected by indirectimmunofluorescence. In agreement with clinical and microbiological diagnosis, the results distinguished betweenhistoplasmosis and coccidioidomycosis patients. In addition, the assay could clearly discriminate between IgM andIgG antibody reactivity. No reactivity was ever observed in the arrays processed with negative control sera. Therefore,this pilot study demonstrates that the \u201cmycoarray\u201d is sensitive and specific enough to discriminate between healthyindividuals and patients with histoplasmosis or coccidioidomycosis. Because of miniaturization and multiparametricity,the new assay cuts costs and processing time. Thus, once clinically validated and implemented as a large-scale array,the \u201cmycoarray\u201d will be ready to be applied to the daily clinical practice
-
The protein “mycoarray”: a novel serological assay for the laboratory diagnosis of primitive endemic mycoses
2011Co-Authors: Ardizzoni Andrea, Baschieri, Maria Cristina, Manca Lidia, Orsi, Carlotta Francesca, C. Venturelli, M. Meacci, Peppoloni Samuele, C. Farina, Blasi ElisabettaAbstract:A protein microarray containing fungal antigens (the \u201cmycoarray\u201d) has been set up to provide rapid and appropriateserodiagnosis of primitive endemic mycoses, an important cause of morbidity and mortality in an increasingly highnumber of patients. The mycoarray consists of three antigen extracts (histoplasmin, Coccidioidin and Coccidioides \u201cTP\u201d)and antibody dilution curves were spotted on microarray slides. The arrays were processed with coccidioidomycosisand histoplasmosis patients\u2019 sera or with control sera and the occurring immunocomplexes were detected by indirectimmunofluorescence. In agreement with clinical and microbiological diagnosis, the results distinguished betweenhistoplasmosis and coccidioidomycosis patients. In addition, the assay could clearly discriminate between IgM andIgG antibody reactivity. No reactivity was ever observed in the arrays processed with negative control sera. Therefore,this pilot study demonstrates that the \u201cmycoarray\u201d is sensitive and specific enough to discriminate between healthyindividuals and patients with histoplasmosis or coccidioidomycosis. Because of miniaturization and multiparametricity,the new assay cuts costs and processing time. Thus, once clinically validated and implemented as a large-scale array,the \u201cmycoarray\u201d will be ready to be applied to the daily clinical practice
-
The protein “mycoarray”: a novel immunoassay for the serological diagnosis of primitive invasive mycoses
ECCMID meeting in Vienna; 10 – 13 April 2010., 2010Co-Authors: Ardizzoni Andrea, Baschieri, Maria Cristina, Manca Lidia, Farina Claudio, Cermelli Claudio, Meacci Marisa, Venturelli Claudia, Blasi ElisabettaAbstract:Objectives. Invasive fungal infections are an important cause of morbidity and mortality in an increasingly higher number of patients, also because of difficulties in providing a rapid and appropriate diagnosis. In some cases, detection of a specific antibody response is a crucial diagnostic tool; however, the available serological assays often provide qualitative results only, their sensitivity and specificity are poor and long time procedures are required. In addition, patients who suffer from an invasive mycosis may have multiple infections likely underestimated by conventional diagnostic approaches. In order to couple the serology of primitive invasive mycoses to the protein microarray technology, a \u201cmycoarray\u201d assay has been designed and set up.Methods. Four antigen extracts (histoplasmin, Coccidioidin, Coccidioides \u201cTP\u201d antigen and aspergillin) and the appropriate controls were spotted in various conditions onto a restricted area of a microscope slide. The printed slides were then incubated with immune sera produced in goat against each single antigen or, subsequently, with human sera (6 from patients affected by primitive invasive mycoses and 7 from healthy individuals). The occurring immunocomplexes were detected by indirect immunofluorescence.Results. The pilot experiments, conducted using the goat immune sera, allowed to establish the optimal spotting conditions for each antigen in terms of both spotting buffer and extracts\u2019 dilution. The \u201cmycoarrays\u201d, obtained by spotting all the fungal antigens with the best condition, were then processed with sera either from patients or control subjects. The reactivity observed in the arrays processed with the patients\u2019 samples was in agreement with the clinical and microbiological diagnosis; no reactivity was ever observed in the arrays processed with the negative control sera.Conclusions. The \u201cprotein mycoarray\u201d is sensitive enough to discriminate between healthy individuals and patients affected by histoplasmosis or coccidioidomycosis. This novel diagnostic tool, because of its intrinsic features, miniaturization and multiparametricity, can contribute to cut out costs and to shorten times-to-results, with the potentiality to be included in the daily clinical practice in the near future
-
The protein \u201cmycoarray\u201d: a novel immunoassay for the serological diagnosis of primitive invasive mycoses
ECCMID meeting in Vienna; 10 \u2013 13 April 2010., 2010Co-Authors: Ardizzoni Andrea, Baschieri, Maria Cristina, Manca Lidia, Farina Claudio, Cermelli Claudio, Meacci Marisa, Venturelli Claudia, Blasi ElisabettaAbstract:Objectives. Invasive fungal infections are an important cause of morbidity and mortality in an increasingly higher number of patients, also because of difficulties in providing a rapid and appropriate diagnosis. In some cases, detection of a specific antibody response is a crucial diagnostic tool; however, the available serological assays often provide qualitative results only, their sensitivity and specificity are poor and long time procedures are required. In addition, patients who suffer from an invasive mycosis may have multiple infections likely underestimated by conventional diagnostic approaches. In order to couple the serology of primitive invasive mycoses to the protein microarray technology, a \u201cmycoarray\u201d assay has been designed and set up.Methods. Four antigen extracts (histoplasmin, Coccidioidin, Coccidioides \u201cTP\u201d antigen and aspergillin) and the appropriate controls were spotted in various conditions onto a restricted area of a microscope slide. The printed slides were then incubated with immune sera produced in goat against each single antigen or, subsequently, with human sera (6 from patients affected by primitive invasive mycoses and 7 from healthy individuals). The occurring immunocomplexes were detected by indirect immunofluorescence.Results. The pilot experiments, conducted using the goat immune sera, allowed to establish the optimal spotting conditions for each antigen in terms of both spotting buffer and extracts\u2019 dilution. The \u201cmycoarrays\u201d, obtained by spotting all the fungal antigens with the best condition, were then processed with sera either from patients or control subjects. The reactivity observed in the arrays processed with the patients\u2019 samples was in agreement with the clinical and microbiological diagnosis; no reactivity was ever observed in the arrays processed with the negative control sera.Conclusions. The \u201cprotein mycoarray\u201d is sensitive enough to discriminate between healthy individuals and patients affected by histoplasmosis or coccidioidomycosis. This novel diagnostic tool, because of its intrinsic features, miniaturization and multiparametricity, can contribute to cut out costs and to shorten times-to-results, with the potentiality to be included in the daily clinical practice in the near future
A.e. Anides-fonseca - One of the best experts on this subject based on the ideXlab platform.
-
Detección de infección por Coccidioides immitis en zonas del estado de Coahuila, México Detection of Coccidioides immitis infection in Coahuila, Mexico
Elsevier España S.L.U., 2005Co-Authors: R. Mondragón-gonzález, L.j. Méndez-tovar, E. Bernal-vázquez, F. Hernández-hernández, R. López-martínez, P. Manzano-gayosso, C. Ríos-rosas, C. Contreras-pérez, A.e. Anides-fonsecaAbstract:La coccidioidomicosis es una micosis inicialmente pulmonar causada por Coccidioides immitis; puede diseminarse principalmente a sistema nervioso central, huesos y piel. En México se desconoce la frecuencia exacta de esta enfermedad. Nuestro objetivo fue determinar, por intradermorreacción y por serología, los casos de infección por C. immitis en 12 comunidades (10 rurales y dos urbanas) atendidas en el Hospital Rural Nº 79 del Instituto Mexicano del Seguro Social (IMSS) del estado de Coahuila, México. Se estudiaron 668 individuos adultos de ambos sexos; se les aplicó 0,1 ml de Coccidioidina por vía intradérmica; después de 72 hs. se midió el diámetro de induración. Fueron seleccionados 180 individuos y a partir del suero se determinaron los niveles de inmunoglobulinas anti-C. immitis por ELISA. Fueron positivos a la Coccidioidina 621 sujetos (93%), frecuencia mucho mayor a la reportada previamente en Coahuila. De los 180 sueros estudiados los promedios de densidad óptica (DO) fueron: IgG1, 1,55; IgG2, 0,94; IgG total, 0,33; IgG3, 0,29; IgG4, 0,27; IgM, 0,08. Los valores de IgG1, IgG2 e IgM comparados con las otras inmunoglobulinas fueron estadísticamente significativos. Los valores de IgG1 e IgG2 sugieren contacto frecuente con los antígenos e incluso probables casos de enfermedad no diagnosticada.Coccidioidomycosis is a mycosis firstly pulmonar caused by Coccidioides immitis; it can be disseminated to central nervous system, bones and skin, principaly. In Mexico, the real frequency of the disease is unknown. The aim of this work was to determine, by skin test and by serology, the infection cases by C. immitis in twelve communities (10 rural and two urban), attended in the Hospital Rural Nº 79 at the Instituto Mexicano del Seguro Social (IMSS) from the Coahuila State, Mexico. Six hundred and sixty eight adult individuals of both sexes were studied, to whom 0.1 ml of Coccidioidin by intradermal route was applied; 72 h after, the induration diameter was measured. One hundred eighty individuals were selected and seric anti-C. immitis immunoglobulins levels were determined by ELISA. Six hundred twenty one individuals (93%) were positive to Coccidioidin, the frequency was much higher than that previously reported in Coahuila. From 180 sera studied, the means of optical density (OD) were: IgG1, 1.55; IgG2, 0.94; total IgG, 0.33; IgG3, 0.29; IgG4, 0.27; IgM, 0.08. The values of IgG1, IgG2 and IgM compared with the other immunoglobulins were statistically significant. The high values of IgG1 and IgG2 suggest frequent contact with the antigen, and probable cases of undiagnosed disease