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Ana Maria Lima De Azeredo-espin - One of the best experts on this subject based on the ideXlab platform.
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Identification and characterization of microRNAs in the screwworm flies Cochliomyia hominivorax and Cochliomyia macellaria (Diptera: Calliphoridae).
Insect molecular biology, 2016Co-Authors: D. F. Paulo, Ana Maria Lima De Azeredo-espin, L. E. C. Canesin, Renato Vicentini, Ana Carolina M. JunqueiraAbstract:MicroRNAs (miRNAs) are small noncoding RNAs that modulate gene expression through post-transcriptional regulation. Here, we report the identification and characterization of miRNAs in two closely related screwworm flies with different feeding habits: Cochliomyia hominivorax and Cochliomyia macellaria. The New World screwworm, C. hominivorax, is an obligatory parasite of warm-blooded vertebrates, whereas the secondary screwworm, C. macellaria, is a free-living organism that feeds on decaying organic matter. Here, the small RNA transcriptomes of adults and third-instar larvae of both species were sequenced. A total of 110 evolutionarily conserved miRNAs were identified, and 10 putative precursor miRNAs (pre-miRNAs) were predicted. The relative expression of six selected miRNAs was further investigated, including miRNAs that are related to reproduction and neural processes in other insects. Mature miRNAs were also characterized across an evolutionary time scale, suggesting that the majority of them have been conserved since the emergence of the Arthropoda [540 million years ago (Ma)], Hexapoda (488 Ma) and Brachycera (195 Ma) lineages. This study is the first report of miRNAs for screwworm flies. We also performed a comparative analysis with the hereby predicted miRNAs from the sheep blowfly, Lucilia cuprina. The results presented may advance our understanding of parasitic habits within Calliphoridae and assist further functional studies in blowflies.
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Development of new polymorphic microsatellite markers for the New World screw-worm Cochliomyia hominivorax (Diptera: Calliphoridae)
Molecular Ecology Notes, 2005Co-Authors: Tatiana Teixeira Torres, Ana Maria Lima De Azeredo-espinAbstract:In this report, we describe the development of seven new polymorphic microsatellite markers for Cochliomyia hominivorax , a parasitic insect pest of primary agricultural and veterinary importance throughout the Neotropics. The number of alleles found ranged from 3 to 13 per locus, with the expected heterozygosity ranging from 0.4220 to 0.9045. The across-taxa amplification of some of these new microsatellite loci was successful in four additional Calliphoridae species. In combination with the 10 polymorphic microsatellite markers previously described, the markers developed here should provide a high resolution for assessing the fine-scale genetic structure of New World screw-worms.
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Isolation and characterization of microsatellite markers in the new world screw‐worm Cochliomyia hominivorax (Diptera: Calliphoridae)
Molecular Ecology Notes, 2004Co-Authors: Tatiana Teixeira Torres, Rosana Pereira Vianello Brondani, José E. Garcia, Ana Maria Lima De Azeredo-espinAbstract:The new world screw-worm, Cochliomyia hominivorax, is one of the most important parasitic insect pests. This species causes myiasis and, consequently, significant losses to livestock industries in neotropical regions. As part of an investigation into the genetic variability and population structure of the new world screw-worm, a set of 10 polymorphic microsatellite markers was isolated from an AC-enriched genomic library. Amplification of the reported loci in 30 screw-worms revealed an average of 6.9 alleles per locus with expected heterozygosities ranging from 0.3831 to 0.8022. Cross-species amplifications suggested that these loci may be useful in other Calliphoridae species.
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characterization of the screwworm flies Cochliomyia hominivorax and Cochliomyia macellaria by pcr rflp of mitochondrial dna
Medical and Veterinary Entomology, 2001Co-Authors: P. Litjens, Ana Cláudia Lessinger, Ana Maria Lima De Azeredo-espinAbstract:Summary The primary screwworm fly Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) is one of the most important insect pests of livestock in neotropical regions, whereas Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae), the secondary screwworm, is of medical and sanitary importance because of its role in the dissemination of pathogens. These two species share morphological similarities and both may occur in the same myiasis, but in different developmental stages. In this work, the usefulness of PCR-RFLP (polymerase chain reaction – restriction fragment length polymorphism) of mitochondrial DNA (mtDNA) for the unambiguous identification of C. hominivorax and C. macellaria was investigated. Two specific regions of mtDNA were amplified: 870 bp from Cytochrome oxidase subunit I and 2100 bp from the A + T rich/12S region from C. hominivorax and C. macellaria specimens from different areas of Brazil. Reliable species-specific PCR-RFLP results were obtained for the CO I region and the A + T rich/12S region using the restriction enzymes Dra I and Ssp I. These results confirm the conservation of CO I diagnostic restriction sites previously reported and demonstrate the usefulness of the control region sequences as an efficient marker for PCR-RFLP identification of Brazilian screwworm flies. The occurrences of intraspecific polymorphic patterns are discussed based on frequencies and potential conflicts for species identification. PCR-RFLP provides a potentially useful method for identifying samples from the areas where these species are monitored.
Tatiana Teixeira Torres - One of the best experts on this subject based on the ideXlab platform.
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Development of new polymorphic microsatellite markers for the New World screw-worm Cochliomyia hominivorax (Diptera: Calliphoridae)
Molecular Ecology Notes, 2005Co-Authors: Tatiana Teixeira Torres, Ana Maria Lima De Azeredo-espinAbstract:In this report, we describe the development of seven new polymorphic microsatellite markers for Cochliomyia hominivorax , a parasitic insect pest of primary agricultural and veterinary importance throughout the Neotropics. The number of alleles found ranged from 3 to 13 per locus, with the expected heterozygosity ranging from 0.4220 to 0.9045. The across-taxa amplification of some of these new microsatellite loci was successful in four additional Calliphoridae species. In combination with the 10 polymorphic microsatellite markers previously described, the markers developed here should provide a high resolution for assessing the fine-scale genetic structure of New World screw-worms.
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isolation and characterization of microsatellite markers in the new world screw worm Cochliomyia hominivorax diptera calliphoridae
Molecular Ecology Notes, 2004Co-Authors: Tatiana Teixeira Torres, Rosana Pereira Vianello Brondani, José E. Garcia, Ana Maria Lima De AzeredoespinAbstract:The new world screw-worm, Cochliomyia hominivorax, is one of the most important parasitic insect pests. This species causes myiasis and, consequently, significant losses to livestock industries in neotropical regions. As part of an investigation into the genetic variability and population structure of the new world screw-worm, a set of 10 polymorphic microsatellite markers was isolated from an AC-enriched genomic library. Amplification of the reported loci in 30 screw-worms revealed an average of 6.9 alleles per locus with expected heterozygosities ranging from 0.3831 to 0.8022. Cross-species amplifications suggested that these loci may be useful in other Calliphoridae species.
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Isolation and characterization of microsatellite markers in the new world screw‐worm Cochliomyia hominivorax (Diptera: Calliphoridae)
Molecular Ecology Notes, 2004Co-Authors: Tatiana Teixeira Torres, Rosana Pereira Vianello Brondani, José E. Garcia, Ana Maria Lima De Azeredo-espinAbstract:The new world screw-worm, Cochliomyia hominivorax, is one of the most important parasitic insect pests. This species causes myiasis and, consequently, significant losses to livestock industries in neotropical regions. As part of an investigation into the genetic variability and population structure of the new world screw-worm, a set of 10 polymorphic microsatellite markers was isolated from an AC-enriched genomic library. Amplification of the reported loci in 30 screw-worms revealed an average of 6.9 alleles per locus with expected heterozygosities ranging from 0.3831 to 0.8022. Cross-species amplifications suggested that these loci may be useful in other Calliphoridae species.
Ana Maria Lima De Azeredoespin - One of the best experts on this subject based on the ideXlab platform.
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isolation and characterization of microsatellite markers in the new world screw worm Cochliomyia hominivorax diptera calliphoridae
Molecular Ecology Notes, 2004Co-Authors: Tatiana Teixeira Torres, Rosana Pereira Vianello Brondani, José E. Garcia, Ana Maria Lima De AzeredoespinAbstract:The new world screw-worm, Cochliomyia hominivorax, is one of the most important parasitic insect pests. This species causes myiasis and, consequently, significant losses to livestock industries in neotropical regions. As part of an investigation into the genetic variability and population structure of the new world screw-worm, a set of 10 polymorphic microsatellite markers was isolated from an AC-enriched genomic library. Amplification of the reported loci in 30 screw-worms revealed an average of 6.9 alleles per locus with expected heterozygosities ranging from 0.3831 to 0.8022. Cross-species amplifications suggested that these loci may be useful in other Calliphoridae species.
José E. Garcia - One of the best experts on this subject based on the ideXlab platform.
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isolation and characterization of microsatellite markers in the new world screw worm Cochliomyia hominivorax diptera calliphoridae
Molecular Ecology Notes, 2004Co-Authors: Tatiana Teixeira Torres, Rosana Pereira Vianello Brondani, José E. Garcia, Ana Maria Lima De AzeredoespinAbstract:The new world screw-worm, Cochliomyia hominivorax, is one of the most important parasitic insect pests. This species causes myiasis and, consequently, significant losses to livestock industries in neotropical regions. As part of an investigation into the genetic variability and population structure of the new world screw-worm, a set of 10 polymorphic microsatellite markers was isolated from an AC-enriched genomic library. Amplification of the reported loci in 30 screw-worms revealed an average of 6.9 alleles per locus with expected heterozygosities ranging from 0.3831 to 0.8022. Cross-species amplifications suggested that these loci may be useful in other Calliphoridae species.
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Isolation and characterization of microsatellite markers in the new world screw‐worm Cochliomyia hominivorax (Diptera: Calliphoridae)
Molecular Ecology Notes, 2004Co-Authors: Tatiana Teixeira Torres, Rosana Pereira Vianello Brondani, José E. Garcia, Ana Maria Lima De Azeredo-espinAbstract:The new world screw-worm, Cochliomyia hominivorax, is one of the most important parasitic insect pests. This species causes myiasis and, consequently, significant losses to livestock industries in neotropical regions. As part of an investigation into the genetic variability and population structure of the new world screw-worm, a set of 10 polymorphic microsatellite markers was isolated from an AC-enriched genomic library. Amplification of the reported loci in 30 screw-worms revealed an average of 6.9 alleles per locus with expected heterozygosities ranging from 0.3831 to 0.8022. Cross-species amplifications suggested that these loci may be useful in other Calliphoridae species.
Rosana Pereira Vianello Brondani - One of the best experts on this subject based on the ideXlab platform.
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isolation and characterization of microsatellite markers in the new world screw worm Cochliomyia hominivorax diptera calliphoridae
Molecular Ecology Notes, 2004Co-Authors: Tatiana Teixeira Torres, Rosana Pereira Vianello Brondani, José E. Garcia, Ana Maria Lima De AzeredoespinAbstract:The new world screw-worm, Cochliomyia hominivorax, is one of the most important parasitic insect pests. This species causes myiasis and, consequently, significant losses to livestock industries in neotropical regions. As part of an investigation into the genetic variability and population structure of the new world screw-worm, a set of 10 polymorphic microsatellite markers was isolated from an AC-enriched genomic library. Amplification of the reported loci in 30 screw-worms revealed an average of 6.9 alleles per locus with expected heterozygosities ranging from 0.3831 to 0.8022. Cross-species amplifications suggested that these loci may be useful in other Calliphoridae species.
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Isolation and characterization of microsatellite markers in the new world screw‐worm Cochliomyia hominivorax (Diptera: Calliphoridae)
Molecular Ecology Notes, 2004Co-Authors: Tatiana Teixeira Torres, Rosana Pereira Vianello Brondani, José E. Garcia, Ana Maria Lima De Azeredo-espinAbstract:The new world screw-worm, Cochliomyia hominivorax, is one of the most important parasitic insect pests. This species causes myiasis and, consequently, significant losses to livestock industries in neotropical regions. As part of an investigation into the genetic variability and population structure of the new world screw-worm, a set of 10 polymorphic microsatellite markers was isolated from an AC-enriched genomic library. Amplification of the reported loci in 30 screw-worms revealed an average of 6.9 alleles per locus with expected heterozygosities ranging from 0.3831 to 0.8022. Cross-species amplifications suggested that these loci may be useful in other Calliphoridae species.