The Experts below are selected from a list of 1194 Experts worldwide ranked by ideXlab platform
Yanjiang Qiao - One of the best experts on this subject based on the ideXlab platform.
-
novel antihypertensive peptides derived from adlay Coix larchryma jobi l var ma yuen stapf glutelin
Molecules, 2017Co-Authors: Liansheng Qiao, Yanling Zhang, Lingzhi Wang, Yanjiang QiaoAbstract:Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identified using nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC50 = 14.19 μmol·L−1) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensive rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and the effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from Coix glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases.
-
a novel antihypertensive derived from adlay Coix larchryma jobi l var ma yuen stapf glutelin
Molecules, 2017Co-Authors: Liansheng Qiao, Yanling Zhang, Lingzhi Wang, Yanjiang QiaoAbstract:Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identifiedusing nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC50 = 14.19 μmol·L-1) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensively rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and blood pressure-lowering effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from Coix glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases.
Liansheng Qiao - One of the best experts on this subject based on the ideXlab platform.
-
new angiotensin converting enzyme inhibitory peptide from Coix prolamin and its influence on the gene expression of renin angiotensin system in vein endothelial cells
Journal of Cereal Science, 2020Co-Authors: Peiyao Chen, Xiaoqian Huo, Liansheng Qiao, Yanling Zhang, Zujun Chen, Lingzhi WangAbstract:Abstract Coix seed, which is a traditional Chinese medicine, has been used to treat hypertension for thousands of years. It has been shown that Coix prolamin peptides display high levels of angiotensin I converting enzyme (ACE) inhibitory activity. Hence, we purified the ACE inhibitory peptides from Coix prolamin hydrolysates and evaluated the influence of the most potent peptide on the renin-angiotensin system (RAS) genes expression in human umbilical vein endothelial cells (HUVECs). In this study, Coix prolamin peptides were sequentially separated by ultrafiltration, ion exchange chromatography, gel filtration chromatography and RP-HPLC, while the peptide structure was analyzed by mass spectrometry. Next, in silico proteolysis, pharmacophore and molecular docking were further applied to screen and optimize the structure of peptides. Finally, a novel ACE inhibitory peptide VDMF was obtained, in which its influence on the gene expression of RAS signaling pathway in AngⅡ-injury HUVECs was evaluated by quantitative real-time PCR. VDMF significantly down-regulated ACE, AngII type 1 receptor (AT1R) and ACE2 mRNA expression in comparation with model group, while up-regulating Mas gene expression. Hence, we obtained a novel antihypertensive candidate that was derived from the Coix peptides, which could involve a multi-modulation mechanism that regulates blood pressure.
-
novel antihypertensive peptides derived from adlay Coix larchryma jobi l var ma yuen stapf glutelin
Molecules, 2017Co-Authors: Liansheng Qiao, Yanling Zhang, Lingzhi Wang, Yanjiang QiaoAbstract:Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identified using nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC50 = 14.19 μmol·L−1) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensive rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and the effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from Coix glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases.
-
a novel antihypertensive derived from adlay Coix larchryma jobi l var ma yuen stapf glutelin
Molecules, 2017Co-Authors: Liansheng Qiao, Yanling Zhang, Lingzhi Wang, Yanjiang QiaoAbstract:Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identifiedusing nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC50 = 14.19 μmol·L-1) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensively rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and blood pressure-lowering effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from Coix glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases.
Chun T Rim - One of the best experts on this subject based on the ideXlab platform.
-
Self-Inductance-Based Metal Object Detection With Mistuned Resonant Circuits and Nullifying Induced Voltage for Wireless EV Chargers
IEEE Transactions on Power Electronics, 2019Co-Authors: Seog Y Jeong, Van X. Thai, Jun H. Park, Chun T RimAbstract:In this paper, a metal object detection (MOD) system, a kind of foreign object detection (FOD), which is based on mistuned resonant circuits and utilizes the variation of self-inductance of a sensing pattern, is newly proposed for wireless electric vehicle (EV) chargers. The sensing pattern that consists of multiple loop coil sets is mounted on the transmitting (Tx) pad of an EV charger, where a loop coil set has two coils connected in series with the opposite polarity to cancel out the induced voltage generated by the Tx coil. Variation of self-inductance of the loop coil set is detected by a parallel-resonant circuit, driven by a current source and operating at near 1 MHz, in order to enhance the resolution of the proposed MOD system. To increase the detection sensitivity of the proposed MOD system, instead of an exact resonant frequency, a mistuned operating frequency near the –3 dB point is utilized for the parallel-resonant circuit. In this way, the proposed MOD system can detect very small metal objects regardless of their position and orientation on the Tx coil without any blind zone. Through simulations and experiments, it is found that the proposed MOD system detects not only horizontal but also standing upright metal objects. A prototype MOD system, operating at 85 kHz to satisfy the standard J2954, was fabricated to verify its feasibility. The results showed that output voltage change of the proposed MOD system becomes 22.7% for a piece of the aluminum foil of 3 × 3 cm2 and 40.9% for 100 Korean Won coin.
-
dual purpose nonoverlapping coil sets as metal object and vehicle position detections for wireless stationary ev chargers
IEEE Transactions on Power Electronics, 2018Co-Authors: Seog Y Jeong, Hyung G Kwak, Gi C Jang, Su Y Choi, Chun T RimAbstract:For commercialization of wireless stationary electric vehicles (EV) chargers, metal object detection (MOD) on a power supply coil and detection of position (DoP) of EVs are needed. In this paper, dual-purpose nonoverlapping coil sets for both MOD and DoP, which detect a variation of magnetic flux on the power supply coil, are newly proposed, where the proposed MOD and DoP methods make no contribution to any power losses. The existence of metal objects on the power supply coil is determined by an induced voltage difference of the nonoverlapping coil sets, whereas the position of the EV is determined by an induced voltage of the nonoverlapping coil sets. A sensing circuit, which has a variable resistor that is different from the conventional overlapping coil for MOD, can make the induced voltage difference zero even when the magnetic flux distribution is distorted by moving the pick-up coil. The proposed nonoverlapping coil sets with the sensing circuit have been demonstrated by simulations and experiments. When metallic coins and aluminum sheets are located on the power supply coil, the induced voltage difference of the coil sets, which is ideally zero without metal objects, significantly increases to 62.8 and 450 mV, respectively, which is more than ten times the value without metal objects throughout experiments. In addition, when the pick-up coil approaches the power supply coil, induced voltage of each coil set increased roughly 1.6 times at 10 cm air gap.
-
metal object detection system with parallel mistuned resonant circuits and nullifying induced voltage for wireless ev chargers
2018 International Power Electronics Conference (IPEC-Niigata 2018 -ECCE Asia), 2018Co-Authors: Seog Y Jeong, Jun H. Park, Van X Thai, Chun T RimAbstract:In this paper, a metal object detection (MOD) system, a kind of foreign object detection (FOD), which is based on mistuned resonant circuits and utilizes variation of self-inductance of a sensing pattern, is newly proposed for wireless electric vehicle (EV) chargers. The sensing pattern that consists of multiple loop coil sets is mounted on the transmitting (Tx) pad of an EV charger, where a loop coil set has two coils connected in series with opposite polarity to cancel out the induced voltage generated by the Tx coil. Variation of self-inductance of the loop coil set is detected by a parallel-resonant circuit, driven by a current source and operating at near 1 MHz. To increase the detection sensitivity of the proposed MOD system, instead of an exact resonant frequency, a mistuned operating frequency near the −3dB point is utilized for the parallel-resonant circuit. Through simulations and experiments, it is found that the proposed MOD system detects not only horizontal but also standing upright metal objects. A prototype MOD system, operating at 85 kHz to satisfy the standard J2954, was fabricated to verify its feasibility. The results showed that output voltage change of the proposed MOD system becomes 22.7 % for a piece of aluminum foil of 3 × 3 cm2 and 40.9 % for 100 Korean Won coin, respectively.
Lingzhi Wang - One of the best experts on this subject based on the ideXlab platform.
-
new angiotensin converting enzyme inhibitory peptide from Coix prolamin and its influence on the gene expression of renin angiotensin system in vein endothelial cells
Journal of Cereal Science, 2020Co-Authors: Peiyao Chen, Xiaoqian Huo, Liansheng Qiao, Yanling Zhang, Zujun Chen, Lingzhi WangAbstract:Abstract Coix seed, which is a traditional Chinese medicine, has been used to treat hypertension for thousands of years. It has been shown that Coix prolamin peptides display high levels of angiotensin I converting enzyme (ACE) inhibitory activity. Hence, we purified the ACE inhibitory peptides from Coix prolamin hydrolysates and evaluated the influence of the most potent peptide on the renin-angiotensin system (RAS) genes expression in human umbilical vein endothelial cells (HUVECs). In this study, Coix prolamin peptides were sequentially separated by ultrafiltration, ion exchange chromatography, gel filtration chromatography and RP-HPLC, while the peptide structure was analyzed by mass spectrometry. Next, in silico proteolysis, pharmacophore and molecular docking were further applied to screen and optimize the structure of peptides. Finally, a novel ACE inhibitory peptide VDMF was obtained, in which its influence on the gene expression of RAS signaling pathway in AngⅡ-injury HUVECs was evaluated by quantitative real-time PCR. VDMF significantly down-regulated ACE, AngII type 1 receptor (AT1R) and ACE2 mRNA expression in comparation with model group, while up-regulating Mas gene expression. Hence, we obtained a novel antihypertensive candidate that was derived from the Coix peptides, which could involve a multi-modulation mechanism that regulates blood pressure.
-
novel antihypertensive peptides derived from adlay Coix larchryma jobi l var ma yuen stapf glutelin
Molecules, 2017Co-Authors: Liansheng Qiao, Yanling Zhang, Lingzhi Wang, Yanjiang QiaoAbstract:Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identified using nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC50 = 14.19 μmol·L−1) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensive rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and the effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from Coix glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases.
-
a novel antihypertensive derived from adlay Coix larchryma jobi l var ma yuen stapf glutelin
Molecules, 2017Co-Authors: Liansheng Qiao, Yanling Zhang, Lingzhi Wang, Yanjiang QiaoAbstract:Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identifiedusing nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC50 = 14.19 μmol·L-1) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensively rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and blood pressure-lowering effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from Coix glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases.
Yanling Zhang - One of the best experts on this subject based on the ideXlab platform.
-
new angiotensin converting enzyme inhibitory peptide from Coix prolamin and its influence on the gene expression of renin angiotensin system in vein endothelial cells
Journal of Cereal Science, 2020Co-Authors: Peiyao Chen, Xiaoqian Huo, Liansheng Qiao, Yanling Zhang, Zujun Chen, Lingzhi WangAbstract:Abstract Coix seed, which is a traditional Chinese medicine, has been used to treat hypertension for thousands of years. It has been shown that Coix prolamin peptides display high levels of angiotensin I converting enzyme (ACE) inhibitory activity. Hence, we purified the ACE inhibitory peptides from Coix prolamin hydrolysates and evaluated the influence of the most potent peptide on the renin-angiotensin system (RAS) genes expression in human umbilical vein endothelial cells (HUVECs). In this study, Coix prolamin peptides were sequentially separated by ultrafiltration, ion exchange chromatography, gel filtration chromatography and RP-HPLC, while the peptide structure was analyzed by mass spectrometry. Next, in silico proteolysis, pharmacophore and molecular docking were further applied to screen and optimize the structure of peptides. Finally, a novel ACE inhibitory peptide VDMF was obtained, in which its influence on the gene expression of RAS signaling pathway in AngⅡ-injury HUVECs was evaluated by quantitative real-time PCR. VDMF significantly down-regulated ACE, AngII type 1 receptor (AT1R) and ACE2 mRNA expression in comparation with model group, while up-regulating Mas gene expression. Hence, we obtained a novel antihypertensive candidate that was derived from the Coix peptides, which could involve a multi-modulation mechanism that regulates blood pressure.
-
novel antihypertensive peptides derived from adlay Coix larchryma jobi l var ma yuen stapf glutelin
Molecules, 2017Co-Authors: Liansheng Qiao, Yanling Zhang, Lingzhi Wang, Yanjiang QiaoAbstract:Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identified using nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC50 = 14.19 μmol·L−1) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensive rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and the effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from Coix glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases.
-
a novel antihypertensive derived from adlay Coix larchryma jobi l var ma yuen stapf glutelin
Molecules, 2017Co-Authors: Liansheng Qiao, Yanling Zhang, Lingzhi Wang, Yanjiang QiaoAbstract:Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identifiedusing nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC50 = 14.19 μmol·L-1) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensively rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and blood pressure-lowering effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from Coix glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases.